Article
Biochemical Research Methods
Bin Yang, Po-Yi Lee, Yi Hua, Bryn Brazile, Susannah Waxman, Fengting Ji, Ziyi Zhu, Ian A. Sigal
Summary: The study introduces instant polarized light microscopy (IPOL) as a quantitative imaging technique for evaluating the microstructure of collagen fibers. Experimental results show that IPOL can achieve quantitative characterization of collagen fiber architecture at high frame rates.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Biotechnology & Applied Microbiology
Michael J. Blair, Kyle P. Quinn
Summary: Quantitative Polarized Light Imaging (QPLI) is a technique used to compute the orientation of collagen fibers. In this paper, a single shot QPLI system (ssQPLI) without moving parts is presented, which can accurately quantify the fiber orientation and quickly provide pixel-wise measurements during mechanical testing. The ssQPLI system utilizes four cameras to simultaneously collect linearly polarized light in four states.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiawei Li, Huiming Jiang, Zhongyang Lv, Ziying Sun, Chaoqun Cheng, Guihua Tan, Maochun Wang, Anlong Liu, Heng Sun, Hu Guo, Fufei Chen, Zizheng Liu, Yuxiang Fei, Yuan Liu, Rui Wu, Xingquan Xu, Wenjin Yan, Qing Jiang, Dongquan Shi
Summary: This study proposed a new strategy for cartilage regeneration by hyalinizing fibrocartilage through stabilizing the cytoskeleton. The use of docetaxel was found to inhibit cartilage fibrosis and promote the regeneration of hyaline cartilage.
Review
Engineering, Multidisciplinary
Liangbin Zhou, Peng Guo, Matteo D'Este, Wenxue Tong, Jiankun Xu, Hao Yao, Martin J. Stoddart, Gerjo J. V. M. van Osch, Kevin Ki-Wai Ho, Zhen Li, Ling Qin
Summary: Articular cartilage (AC) is a flexible connective tissue located on the bone surface in the joints. AC defects are common in physically active individuals. Functionalized hydrogels have emerged as promising substitutes for damaged cartilage in AC repair due to their favorable properties. This article introduces the composition, structure, and function of AC and its defects, and reviews the design and fabrication of functionalized hydrogels for AC repair.
Article
Optics
Po-Yi Lee, Hannah Schilpp, Nathan Naylor, Simon C. Watkins, Bin Yang, Ian Sigal
Summary: Collagen architecture plays a crucial role in determining the biomechanical properties of the eye. In this study, we introduce a variation of instant polarized light microscopy (IPOL ir) that allows for better characterization of collagen fiber orientation and retardance. IPOL ir has several advantages over IPOL, including the ability to distinguish orthogonal collagen fiber orientations, faster imaging speed, visualization of non-birefringent tissues, and reduced sensitivity to imperfectly collimated light. Overall, IPOL ir provides a higher resolution and deeper insight into ocular biomechanics and eye physiology and pathology.
OPTICS AND LASERS IN ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Jay M. Patel, Claudia Loebel, Kamiel S. Saleh, Brian C. Wise, Edward D. Bonnevie, Liane M. Miller, James L. Carey, Jason A. Burdick, Robert L. Mauck
Summary: This study demonstrates that a hyaluronic acid hydrogel system can interdigitate with and promote sealing of degenerated cartilage, restoring fluid pressurization within the tissue and preventing further fluid flow and matrix loss from the defect surface. Additionally, the hydrogel therapy can guide localized extracellular matrix deposition through presenting chemo mechanical cues, showing therapeutic potential for damaged cartilage treatment across different length scales.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Fernando Canonici, Cristiano Cocumelli, Antonella Cersini, Daniele Marcoccia, Alessia Zepparoni, Annalisa Altigeri, Daniela Caciolo, Cristina Roncoroni, Valentina Monteleone, Elisa Innocenzi, Cristian Alimonti, Paola Ghisellini, Cristina Rando, Eugenia Pechkova, Roberto Eggenhoeffner, Maria Teresa Scicluna, Katia Barbaro
Summary: Cartilage injuries in animals and humans can lead to the progression of osteoarthritis and joint deterioration. A new experimental protocol for treating osteochondral lesions involves isolating chondrocytes from equine hyaline cartilage and using them to repair equine joint cartilage injuries. The chondrocytes, taken from autologous tracheal cartilage, are grown in a laboratory and implanted arthroscopically into the injured joint. Histological and immunohistochemical evaluation of the treated cartilage after 8 and 13 months shows progressive maturation, with tissue comparable to native articular cartilage detected after 24 months.
Article
Anatomy & Morphology
Hannah Mantebea, Syeda Batool, Amanveer Singh, Mohammed Hammami, Farid Badar, Yang Xia
Summary: This study compared the structural and cellular features between immature and mature articular cartilage using microscopic MRI and polarized light microscopy. The results revealed differences in thickness and relaxation values, as well as the presence of a tidemark in mature cartilage. These findings are important for studying degenerative diseases such as osteoarthritis.
JOURNAL OF ANATOMY
(2022)
Article
Engineering, Biomedical
Anna Puiggali-Jou, Riccardo Rizzo, Angela Bonato, Philipp Fisch, Simone Ponta, Daniel M. Weber, Marcy Zenobi-Wong
Summary: Tissue engineering methods have potential for restoring cartilage function, but reproducing cartilage zonal architecture is still a challenge. This study demonstrates that Filamented Light biofabrication can significantly improve cartilage tissue maturity by generating constructs with zonal architecture and native-like mechanical properties.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Kai Qiao, Qi Chen, Yiguo Cao, Jie Li, Gang Xu, Jiaqing Liu, Xiaolin Cui, Kang Tian, Weiguo Zhang
Summary: Cartilage lesions and degenerative joint diseases are two leading contributors to global disability, with current cell-based therapies facing limitations such as high tumorigenicity risk and low retention rate. Non-stem cell-based therapy, particularly extracellular vesicles (EVs), have gained popularity as they have been shown to achieve therapeutic effects similar to stem cells in cartilage tissue engineering. Further research on the therapeutic role of EVs in these conditions is warranted, given their potential to offer effective treatment options in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Yang Zhao, Qianhua Ou, Yu Cai, Guangfeng Ruan, Yan Zhang, Changhai Ding
Summary: Currently available therapeutics for knee osteoarthritis (KOA) only provide symptomatic relief or surgical replacement. However, experimental intra-articular (IA) drugs are being developed and hold great potential as future disease-modifying treatments. These drugs target cartilage repair, cellular homeostasis, cellular senescence, and pain control, and have the advantage of IA administration.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2023)
Article
Orthopedics
Tony Chen, Caroline Brial, Moira McCarthy, Russell F. Warren, Suzanne A. Maher
Summary: The study found that femoral osteochondral defects cause a redistribution of contact stress during simulated gait. The results showed that polyvinyl alcohol implants combined with porous metal bases can significantly improve joint contact mechanics, especially the implants consisting of a 20% PVA disk mounted on a pTi base demonstrated significant improvements in most parameters.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Biology
Anand O. Masson, Bryce Besler, W. Brent Edwards, Roman J. Krawetz
Summary: Characterizing the biomechanical properties of mouse cartilage is crucial for understanding tissue homeostasis and degeneration. This study presents a 3D automated surface mapping system and methodology that allows for mechanical characterization with high spatial resolution. The technique comprehensively characterizes cartilage function in mouse models and has the potential to improve our understanding of tissue structure-function interplay.
Article
Engineering, Biomedical
Robert J. Elkington, Richard M. Hall, Andrew R. Beadling, Hemant Pandit, Michael G. Bryant
Summary: To preserve the function of synovial joint counter-face during osteochondral defect repairs, it is important to develop surfaces optimized for healthy cartilage function. In this study, PEEK surfaces were modified with a hydrophilic coating (SPMK-g-PEEK) to provide a low friction and compliant sliding interface. The results showed that SPMK-g-PEEK maintained low friction and minimized forces on the cartilage interface, providing an enhanced cartilage counter-surface.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Chao Jia, Feng Jiang, Piao Hu, Yudi Kuang, Shuaiming He, Tian Li, Chaoji Chen, Alan Murphy, Chunpeng Yang, Yonggang Yao, Jiaqi Dai, Christopher B. Raub, Xiaolong Luo, Liangbing Hu
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Biochemical Research Methods
Van K. Lam, Thanh Nguyen, Thuc Phan, Byung-Min Chung, George Nehmetallah, Christopher B. Raub
Article
Biochemical Research Methods
R. N. Huynh, B. Pesante, G. Nehmetallah, C. B. Raub
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Biotechnology & Applied Microbiology
Ruby N. Huynh, Manal Yousof, Khanh L. Ly, Farai C. Gombedza, Xiaolong Luo, Bidhan C. Bandyopadhyay, Christopher B. Raub
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biochemical Research Methods
Van K. Lam, Pooja Sharma, Thanh Nguyen, Georges Nehmetallah, Christopher B. Raub, Byung Min Chung
Article
Engineering, Electrical & Electronic
Santiago O. Correa, Xiaolong Luo, Christopher B. Raub
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2020)
Article
Engineering, Biomedical
Ali Mohammadabadi, Ruby N. Huynh, Aniket S. Wadajkar, Rena G. Lapidus, Anthony J. Kim, Christopher B. Raub, Victor Frenkel
PHYSICS IN MEDICINE AND BIOLOGY
(2020)
Article
Optics
Brad Bazow, Thuc Phan, Thanh Nguyen, Christopher Raub, George Nehmetallah
Summary: Research in digital holography has significantly expanded in recent years, with potential applications in various fields including bioengineering. This study demonstrates a high-fidelity simulation of holographic recording, using numerical methods to accurately predict optical interference and aberrations. The long-term goal is to utilize simulated holograms for training machine-learning algorithms to classify relevant objects recorded through different holographic setups.
Article
Biochemical Research Methods
Ruby N. Huynh, George Nehmetallah, Christopher B. Raub
Summary: Articular cartilage birefringence is related to the zonal architecture primarily consisting of type II collagen, and Mueller matrix imaging can be used for assessing cartilage repair and degeneration. Polar decomposition is applied to extract optical parameters from experimental Mueller matrices, with performance confirmed by simulations.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Optics
Brad Bazow, Thuc Phan, Christopher B. Raub, George Nehmetallah
Summary: This study utilized deep learning convolutional neural networks for computational phase synthesis, achieving high-speed simultaneous phase estimates on different wavelengths and single-shot estimates of the integral refractive index without increasing the complexity of the experiment.
Article
Biochemical Research Methods
Van K. Lam, Thuc Phan, Khanh Ly, Xiaolong Luo, George Nehmetallah, Christopher B. Raub
Summary: In this study, a dual-modality quantitative phase and polarization microscope was designed to study the interaction of cells with biomaterials. The results from cell-seeded collagen hydrogels and electrofabricated chitosan membranes demonstrated the accuracy and feasibility of this imaging system.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Engineering, Biomedical
Khanh L. Ly, Xiaolong Luo, Christopher B. Raub
Summary: Oral mucositis is a common complication in patients with head and neck cancer undergoing chemotherapy and radiation treatment. This study presents a microfluidic model of oral mucositis that mimics the induction and resolution mechanisms of the condition.
Article
Materials Science, Multidisciplinary
Khanh L. Ly, Christopher B. Raub, Xiaolong Luo
MATERIALS ADVANCES
(2020)
Article
Materials Science, Biomaterials
Piao Hu, Christopher B. Raub, John S. Choy, Xiaolong Luo
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Biochemical Research Methods
Van K. Lam, Thanh C. Nguyen, Byung M. Chung, George Nehmetallah, Christopher B. Raub