Review
Immunology
Pan Luo, Qiling Yuan, Xianjie Wan, Mingyi Yang, Peng Xu
Summary: The pathological manifestations of osteoarthritis (OA) involve destruction of articular cartilage, synovial sac thickening, subchondral osteosclerosis, and osteophyte formation. Various cell types, including chondrocytes, osteoblasts, osteoclasts, synovial fibroblasts, T cells, macrophages, and mesenchymal stem cells (MSCs), are involved in OA. Immune cells, especially dendritic cells and macrophages, play significant roles in the pathogenesis of OA. Additionally, adaptive immune cells such as T-cell subsets, B-cell subsets, and NK cells are important in OA pathogenesis. MSCs not only play a key role in maintaining the dynamic balance and repairing OA tissue but also have immunomodulatory properties. Therefore, MSCs have potential as a therapeutic agent for OA. This review summarizes the effects of immune cells and cytokines on different cells in OA, aiming to identify new therapeutic targets based on the immune system and cytokine activity sites to prevent OA development.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Michela Mocchi, Elia Bari, Silvia Dotti, Riccardo Villa, Priscilla Berni, Virna Conti, Maurizio Del Bue, Gian Paolo Squassino, Lorena Segale, Roberto Ramoni, Maria Luisa Torre, Sara Perteghella, Stefano Grolli
Summary: The study demonstrates the safety and potential efficacy of canine mesenchymal stromal cells-secretome in treating osteoarthritis through both in vitro experiments and clinical trials on dogs.
Article
Biochemistry & Molecular Biology
Chuncha Bao, Chengqi He
Summary: Osteoarthritis is a common chronic painful disease characterized by articular cartilage degeneration, and MSC-derived exosomes are considered as a promising alternative therapy for OA by promoting cartilage repair through delivering various bioactive molecules.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Cell & Tissue Engineering
Mengqin Yuan, Xue Hu, Lichao Yao, Yingan Jiang, Lanjuan Li
Summary: Mesenchymal stem cell (MSC) transplantation is an effective strategy for treating end-stage liver disease by regenerating tissues and self-renewing to repair the liver. However, the low homing efficiency of MSCs after systemic administration is a major limitation in its application for liver disease treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Dimitrios Kouroupis, Diego Correa
Summary: The detrimental effects of conventional 2D MSC expansion have led to the development of novel techniques, such as 3D culturing, to enhance the functionality and efficacy of MSCs. MSC 3D spheroids exhibit enhanced medicinal signaling activities and improved homing and survival capacities, demonstrating safety and efficacy in preclinical studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sree Samanvitha Kuppa, Hyung Keun Kim, Ju Yeon Kang, Seok Cheol Lee, Jong Keun Seon
Summary: Osteoarthritis is a low-grade inflammatory disorder of the joints, but there are limited treatment options currently available. This article explores the use of mesenchymal stem cells and their secreted factors for intervention in osteoarthritis, with a focus on the polarization of macrophages to reduce inflammation and symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Jing Peng, Zhiyuan Mao, Yang Liu, Yang Tian, Qinjie Leng, Jian Gu, Rui Tan
Summary: This study confirms that 12-Epi-Napelline is effective in the treatment of osteoarthritis and can induce BMSCs to secrete growth factors that promote chondrocyte repair.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Alasdair G. Kay, James M. Fox, James P. Hewitson, Andrew P. Stone, Sophie Robertson, Sally James, Xiao-nong Wang, Elizabeth Kapasa, Xuebin B. Yang, Paul G. Genever
Summary: The heterogeneity of bone marrow mesenchymal stromal cells (MSCs) poses challenges for their clinical development. A subtype of MSCs characterized by CD317 expression has been identified, and they show a constitutive interferon signature linked to human disease. CD317(pos) MSCs induce tissue damage in a skin inflammation model, while CD317(neg) MSCs have no effect. CD317(neg) MSCs can suppress the proliferation of activated human T cells, while CD317(pos) MSCs increase the polarization towards pro-inflammatory Th1 cells. CD317(neg) and CD317(pos) MSCs can suppress leukocyte recruitment, but only CD317(neg) MSCs suppress macrophage numbers.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Engineering, Biomedical
Calvin Chang, Jerry Yan, Zhicheng Yao, Chi Zhang, Xiaowei Li, Hai-Quan Mao
Summary: This review focuses on the bioactivity and secretome of mesenchymal stem cells, as well as the engineering approaches to tune the MSC secretome. It also discusses the recent advances in biomaterials-based therapeutic strategies for delivering MSCs and MSC-derived secretome in extracellular vesicles, along with their advantages and challenges.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Miriam Morente-Lopez, Rocio Mato-Basalo, Sergio Lucio-Gallego, Lucia Silva-Fernandez, Alba Gonzalez-Rodriguez, Fco Javier De Toro, Juan A. Fafian-Labora, Maria C. Arufe
Summary: This study found that extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) modified to inhibit the expression of miR-21 have a higher therapeutic potential in treating osteoarthritis (OA). These EV effectively reduced inflammation in an OA animal model compared to MSC alone, and their therapeutic effect was validated through proteomic and genomic techniques.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Meng Feng, Wenguang Liu, Jing Ding, Yusheng Qiu, Qian Chen
Summary: Hedgehog (HH) signaling is critical in osteoarthritis (OA) pathogenesis, mediating the interaction between OA mesenchymal stromal cells (OA-MSCs) and chondrocytes. Sonic Hedgehog (SHH) acts as a key mediator in promoting MSC growth, aging, low-grade inflammation, replicative senescence, and inducing OA-MSC-induced chondrocyte catabolism and apoptosis through a pro-inflammatory microenvironment in human OA cartilage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Parsa Rezvanian, Aroa Alvarez-Lopez, Raquel Tabraue-Rubio, Rafael Daza, Luis Colchero, Manuel Elices, Gustavo V. Guinea, Daniel Gonzalez-Nieto, Jose Perez-Rigueiro
Summary: Titanium substrates were functionalized by binding fibronectin covalently, and the impact of this extracellular matrix protein on the material surface was evaluated using mesenchymal stem cell (MSC) cultures. This was achieved through the deposition of a thin film with a high surface density of amine groups on the material using the activation vapor silanization (AVS) technique, followed by the covalent binding of fibronectin to the amine groups. The study demonstrated that functionalized samples exhibited improved initial cell adhesion and increased cell area, indicating the potential to modulate the organism's response to implants by covalently binding extracellular matrix proteins.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Jay M. McKinney, Krishna A. Pucha, Thanh N. Doan, Lanfang Wang, Laura D. Weinstock, Benjamin T. Tignor, Kelsey L. Fowle, Rebecca D. Levit, Levi B. Wood, Nick J. Willett
Summary: The study evaluated the effects of sodium alginate microencapsulation on human MSCs in treating OA, and found that encapsulated MSCs can reduce cartilage degeneration and bone remodeling. In response to IL-1 beta stimulation, encapsulated MSCs showed a targeted secretory response, while nonencapsulated MSCs showed an indiscriminate increase in cytokines.
ACTA BIOMATERIALIA
(2022)
Review
Sport Sciences
Arco C. van der Vlist, Marinus Winters, Adam Weir, Clare L. Ardern, Nicky J. Welton, Deborah M. Caldwell, Jan A. N. Verhaar, Robert-Jan De Vos
Summary: Research on treatments for Achilles tendinopathy suggests that exercise therapy may be an effective treatment option with similar effectiveness to other treatments.
BRITISH JOURNAL OF SPORTS MEDICINE
(2021)
Article
Biochemical Research Methods
Z. Demir, S. Bahmany, C. Bethlehem, J. van Oldenrijk, P. K. Bos, B. C. P. Koch
Summary: Total hip and knee arthroplasty have successful outcomes, but a small percentage of patients may suffer from postoperative periprosthetic joint infections. Standard treatment involves staged arthroplasty exchange with antibiotic therapy playing a crucial role. A validated UPC2-MS/MS method for quantification of antibiotics in synovial fluid was described, providing important insights for future PK/PD studies in determining exposure of antibiotics at the site of PJIs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
K. Van der Heiden, H. E. Barrett, E. J. Meester, K. van Gaalen, B. J. Krenning, F. J. Beekman, E. de Blois, J. de Swart, H. J. M. Verhagen, A. van der Lugt, J. P. Norenberg, M. de Jong, M. R. Bernsen, F. J. H. Gijsen
Summary: This study found that calcification and inflammation coexist in human carotid plaques, with different degrees of inflammation associated with different levels of calcification. By combining both imaging modalities and considering plaque phenotype, high-risk unstable plaques with little or no calcification could be accurately identified for diagnostic benefit.
JOURNAL OF NUCLEAR CARDIOLOGY
(2022)
Article
Orthopedics
Ruurd J. A. Kuiper, Ralph J. B. Sakkers, Marijn van Stralen, Vahid Arbabi, Max A. Viergever, Harrie Weinans, Peter R. Seevinck
Summary: Semantic segmentation of bone from lower extremity CT scans has been shown to be beneficial for visualization, diagnosis, and surgical planning in orthopaedics. In this study, the researchers developed a cascaded deep learning approach with a multi-stage V-net, which achieved accurate and fast segmentation of lower extremity bones. The method outperformed the state-of-the-art nnU-net in terms of training time, inference time, and GPU memory requirements. Morphometric measurements performed on the automatic segmentations showed good correlation with the manual segmentations. Overall, this approach showed promise for clinical applications.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Medicine, General & Internal
Qiuke Wang, Marianne F. Mol, P. Koen Bos, Desiree M. J. Dorleijn, Marijn Vis, Jacobijn Gussekloo, Patrick J. E. Bindels, Jos Runhaar, Sita M. A. Bierma-Zeinstra
Summary: This study compared the efficacy of intramuscular (IM) glucocorticoid injection and intra-articular (IA) glucocorticoid injection in reducing knee pain for patients with knee osteoarthritis. The results showed that at 4 weeks, IM glucocorticoid injection was less effective in reducing pain compared to IA injection, but at 8 and 24 weeks, IM injection was noninferior to IA injection. There were no significant differences in all secondary outcomes between the two injection methods.
Article
Orthopedics
Joshua M. Bonsel, Lichelle Groot, Abigael Cohen, Jan A. N. Verhaar, Maaike G. J. Gademan, Anneke Spekenbrink-spooren, Gouke J. Bonsel, Max Reijman
Summary: During the COVID-19 lockdown, THA patients had slightly worse preoperative PROMs, indicating the selection of patients with greater urgency. Postoperative PROMs in both THA and TKA patients showed minimal differences, which were likely not clinically relevant overall.
Article
Surgery
B. A. T. D. van der Gronde, T. P. C. Schlosser, J. H. J. van Erp, T. E. Snijders, R. M. Castelein, H. Weinans, A. de Gast
Summary: This systematic review examined the relationship between sagittal spinopelvic characteristics and implant dislocations after primary THA. The study found that several sagittal spinopelvic patient characteristics were related to THA dislocations, and the associated risks were greater than for other patient and surgery-related factors. Further research is needed to determine which characteristics and parameters should be considered in patients undergoing primary THA.
Article
Emergency Medicine
Saskia H. Van Bergen, Esther M. M. Van Lieshout, Kiran C. Mahabier, Alexandra J. L. M. Geraerds, Suzanne Polinder, Dennis Den Hartog, Michael H. J. Verhofstad
Summary: The study aimed to determine the cost-utility and cost-effectiveness of operative versus nonoperative treatment for humeral shaft fractures. The results showed that operative treatment had higher total costs and QALYs, with an ICUR exceeding the threshold. However, the cost per clinically meaningful difference in DASH was much lower, suggesting that operative treatment for these fractures is cost-effective.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Adriana A. S. Tavares, Laura Mezzanotte, Wendy McDougald, Monique R. Bernsen, Christian Vanhove, Markus Aswendt, Giovanna D. Ielacqua, Felix Gremse, Carmel M. Moran, Geoff Warnock, Claudia Kuntner, Marc C. Huisman
Summary: This study surveyed and summarized the current status of preclinical imaging standardization through community survey and forum discussions. The results showed that there are still challenges in standardizing preclinical imaging techniques, with significant variations in data acquisition, processing, and reporting, and limited awareness of international guidelines for preclinical (imaging) research practices.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Editorial Material
Medicine, General & Internal
Monique R. Bernsen, Wendy McDougald, Laura Mezzanotte, Carmel M. Moran, Adriana Tavares, Louise van der Weerd
FRONTIERS IN MEDICINE
(2022)
Article
Orthopedics
M. C. Koper, R. W. A. Spek, M. Reijman, E. M. van Es, S. J. Baart, J. A. N. Verhaar, P. K. Bos
Summary: The study aims to determine the correlation between increasing serum cobalt and chromium levels and decreasing Harris Hip Score (HHS) and Hip disability and Osteoarthritis Outcome Score (HOOS) in patients who received the Articular Surface Replacement (ASR) hip resurfacing arthroplasty (HRA), as well as evaluate the ten-year revision rate and the influence of sex, inclination angle, and Co level.
BONE & JOINT JOURNAL
(2023)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
S. van Tiel, A. Hoefter, J. Haeck, F. Gremse, M. Bernsen
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
M. Bernsen, M. Aswendt, F. Gremse, M. C. Huisman, G. D. Ielacqua, W. A. McDougald, L. Mezzanotte, C. M. Moran, G. Warnock, C. Kuntner, A. A. S. Tavares, C. Vanhove
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Kelly Warmink, Jaqueline L. Rios, Devin R. van Valkengoed, Nicoline M. Korthagen, Harrie Weinans
Summary: This study compares joint damage in different rat strains in response to a high-fat, high-sucrose diet. The results show that both rat strains have similar metabolic changes, but only Sprague Dawley rats experience a decrease in trabecular bone volume similar to osteoporosis. Osteophyte count and local joint inflammation are higher in Sprague Dawley rats, while cartilage degeneration and systemic inflammatory markers are similar between the rat strains. This suggests that the two rat strains may represent different joint damage phenotypes, potentially reflecting different phenotypes of osteoarthritis observed in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)