Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Xin Yao, Yicun Hu, Maoqiang Lin, Kaichen Peng, Peng Wang, Yanbing Gao, Xidan Gao, Taowen Guo, Xiaobo Zhang, Haiyu Zhou
Summary: RADA16 is a peptide-based biomaterial that can quickly form a 3D structure with good biocompatibility and ease of modification, making it suitable for tissue engineering. However, its weak mechanical properties and poor hydrophilicity are limitations that can be overcome through modification.
Article
Orthopedics
Nobuhiro Okuno, Shuhei Otsuki, Jo Aoyama, Kosuke Nakagawa, Tomohiko Murakami, Kuniaki Ikeda, Yoshinobu Hirose, Hitoshi Wakama, Tomohiro Okayoshi, Yoshinori Okamoto, Yoshiaki, Masashi Neo
Summary: The study demonstrated that the peptide hydrogel scaffold KI24RGDS can promote repair and regeneration of meniscus in a rabbit model, showing significant healing effects. The hydrogel is highly biocompatible and biodegradable, mimicking the three-dimensional structure of the extracellular matrix and promoting cell adhesion and proliferation.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Polymer Science
Kanyakorn Riewruja, Alyssa M. M. Aguglia, Sophie Hines, Meagan J. J. Makarcyzk, Sittisak Honsawek, Hang Lin
Summary: This study successfully enhanced the mechanical strength of gelatin/hyaluronic acid (GH) scaffolds by infiltrating polyethylene glycol diacrylate (PEGDA) and photoinitiators. The PEG-GH hybrid scaffolds displayed good integration capacity and provide a new tool for the repair of chondral injury.
Article
Engineering, Biomedical
Pingping Yuan, Zhiye Li, Bo Shao, Tian Zeng, Xiaopeng Wu, Yinggang Wang, Yimin Zhao, Wei Wu
Summary: In this study, BMSCs pre-conditioned in a starving environment were found to enhance the anti-apoptosis potential of co-transplanted chondrocytes, and extracellular vesicles derived from starving BMSCs played essential roles in improving apoptosis and regulating autophagy of chondrocytes. In vivo studies confirmed that these EVs significantly improved the survival of chondrocyte bricks.
ACTA BIOMATERIALIA
(2022)
Article
Orthopedics
Gustavo M. Zanotto, Paul Liesbeny, Myra Barret, Hannah Zlotnick, Eliot Frank, Alan J. Grodzinsky, David D. Frisbie
Summary: The study aimed to evaluate whether microfracture augmentation combined with trypsin treatment and functionalized hydrogel could improve cartilage repair quality in an equine model. The results demonstrated that this combination treatment significantly improved functional outcome parameters and proteoglycan content in the repaired tissue.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Engineering, Biomedical
Yucong Li, Linlong Li, Ye Li, Lu Feng, Bin Wang, Ming Wang, Haixing Wang, Meiling Zhu, Yongkang Yang, Erik I. Waldorff, Nianli Zhang, Ingmar Viohl, Sien Lin, Liming Bian, Wayne Yuk-Wai Lee, Gang Li
Summary: In this study, a new tissue engineering strategy for cartilage defect treatment was proposed using an injectable self-healing adhesive hydrogel and pulsed electromagnetic field (PEMF) treatment. The results demonstrated the superior clinical potential of this strategy in promoting chondrogenesis and cartilage repair.
BIOACTIVE MATERIALS
(2023)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Materials Science, Biomaterials
Neda Dadgar, Ali Ghiaseddin, Shiva Irani, Shahram Rabbani, Seyed Hossein Ahmadi Tafti, Mina Soufizomorrod, Masoud Soleimani
Summary: The study improved cartilage lesion by transplanting chondrocytes in a scaffold, achieving a breakthrough in tissue engineering. The scaffold showed good biocompatibility and mechanical properties in in vitro and in vivo tests, with cells exhibiting good proliferation on the scaffold.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Pharmacology & Pharmacy
Asma Sepahdar, Abolfazl Nazbar, Saeed Bahadorikhalili, Ghassem Rezaei, Mohammad Ali Shokrgozar, Mohammad Mehdi Dehghan, Hamid Akbari Javar, Shahin Bonakdar
Summary: This paper presents a novel composite scaffold for the regeneration of cartilage tissue. The scaffold is made of a combination of a hydrogel and a controlled release drug delivery system. The release of kartogenin, a chondrogenic-inducing drug, from the scaffold is successfully controlled. The scaffold also demonstrates a porous structure and shows good performance in inducing chondrogenic differentiation of stem cells.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Wenhui Chu, Gaowei Hu, Lin Peng, Wei Zhang, Zhe Ma
Summary: The study demonstrated that adCDMs, derived from antler decellularized cartilage, are rich in collagen and GAGs, with good biosafety and biocompatibility. When implanted into rabbit articular cartilage defects, adCDMs showed excellent therapeutic effects in promoting cartilage regeneration. These findings highlight the potential of adCDMs as a novel source for developing cartilage-regenerating repair materials.
JOURNAL OF BIOLOGICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Jie Xu, Huan Fang, Ya Su, Yue Kang, Deli Xu, Yuen Yee Cheng, Yi Nie, Hong Wang, Tianqing Liu, Kedong Song
Summary: Finding a bioink suitable for 3D bioprinting that can mimic the microenvironment of native skin and prevent bacterial infection remains a major challenge in skin tissue engineering. In this study, a tissue-specific extracellular matrix-based bioink was prepared, and a novel scaffold was obtained using an extrusion 3D bioprinting technology and dynamic hydrogen bonding method. The hybrid scaffold exhibited excellent properties including antibacterial activity, hemostatic effect, and biocompatibility.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Pinxue Li, Liwei Fu, Zhiyao Liao, Yu Peng, Chao Ning, Cangjian Gao, Daxu Zhang, Xiang Sui, Yunfeng Lin, Shuyun Liu, Chunxiang Hao, Quanyi Guo
Summary: This study developed a promising cartilage regenerative system using a chitosan/3D-printed PCL hybrid scaffold containing SMSCs and recruiting TFNA, which greatly improved cartilage defect repair through enhanced cell proliferation and chondrogenesis.
Article
Materials Science, Biomaterials
Min Gong, Jiachen Sun, Guoming Liu, Lang Li, Shuang Wu, Zhou Xiang
Summary: The study introduced a graphene oxide-modified 3D acellular cartilage extracellular matrix scaffold for cartilage repair, which improved the biomechanical performance of the scaffold and promoted cell proliferation and differentiation. In vivo experiments in rats and rabbits demonstrated excellent biocompatibility and osteogenic response of the composite scaffold, showcasing its potential in cartilage tissue engineering and injury treatment.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Engineering, Biomedical
Fangang Meng, Zibo Yang, Dianbo Long, Minghui Gu, Mei Shang, Anyu Zeng, Xingzhao Wen, Yueran Xue, Xiaoyi Zhao, Aishan He
Summary: The molecular weight of hyaluronan affects the chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) seeded on TCP-COL-HA scaffolds, with higher molecular weight showing better chondrogenic induction.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Editorial Material
Orthopedics
A. M. Malfait, R. E. Miller
OSTEOARTHRITIS AND CARTILAGE
(2020)
Review
Orthopedics
R. J. Miller, A-M Malfait, R. E. Miller
OSTEOARTHRITIS AND CARTILAGE
(2020)
Article
Orthopedics
R. E. Miller, P. B. Tran, S. Ishihara, D. Syx, D. Ren, R. J. Miller, A. M. Valdes, A. M. Malfait
OSTEOARTHRITIS AND CARTILAGE
(2020)
Article
Anesthesiology
Delfien Syx, Rachel E. Miller, Alia M. Obeidat, Phuong B. Tran, Robin Vroman, Zoe Malfait, Richard J. Miller, Fransiska Malfait, Anne-Marie Malfait
Review
Pharmacology & Pharmacy
Anne-Marie Malfait, Rachel E. Miller, Joel A. Block
PHARMACOLOGY & THERAPEUTICS
(2020)
Article
Rheumatology
Anne-Marie Malfait, Rachel E. Miller, Richard J. Miller
Summary: Osteoarthritis is a major source of chronic pain worldwide, but current pharmacological interventions do not meet patients' needs. The neurotrophin NGF plays a key role in the pathogenic processes of osteoarthritis, with neutralizing antibodies against NGF currently in clinical trials. Neuronal pathways are modified by interaction with degenerating joint tissues, providing a substrate for identifying potential interventions.
RHEUMATIC DISEASE CLINICS OF NORTH AMERICA
(2021)
Article
Anesthesiology
Dale George, Sandra Hackelberg, Nirupa Jayaraj, Dongjun Ren, Seby Edaserry, Craig Rathwell, Rachel Miller, Jeffery Savas, Richard Miller, Daniela Maria Menichella, Anne-Marie Malfait
Summary: This research identified elevated mitochondrial fission proteins and increased calcium signaling in dorsal root ganglion (DRG) neurons in a mouse model of painful diabetic neuropathy (PDN). By selectively deleting the mitochondrial calcium uniporter, the study demonstrated the restoration of normal mitochondrial morphology, prevention of axonal degeneration, and reversal of mechanical allodynia in the PDN mouse model. These findings indicate the potential of targeting calcium entry into nociceptor mitochondria as an effective therapeutic approach for PDN.
Review
Genetics & Heredity
Fransiska Malfait, Marlies Colman, Robin Vroman, Inge De Wandele, Lies Rombaut, Rachel E. Miller, Anne-Marie Malfait, Delfien Syx
Summary: Chronic pain is a common complaint in Ehlers-Danlos syndromes patients, but poorly studied. Current treatment strategies do not provide adequate pain relief, highlighting an important unmet medical need in EDS.
AMERICAN JOURNAL OF MEDICAL GENETICS PART C-SEMINARS IN MEDICAL GENETICS
(2021)
Editorial Material
Orthopedics
R. E. Miller, A-M Malfait
OSTEOARTHRITIS AND CARTILAGE
(2021)
Review
Genetics & Heredity
Robin Vroman, Anne-Marie Malfait, Rachel E. Miller, Fransiska Malfait, Delfien Syx
Summary: The Ehlers-Danlos syndromes are a group of heritable connective tissue disorders characterized by skin hyperextensibility, joint hypermobility, and tissue fragility. Animal models have been instrumental in studying the pathophysiological aspects associated with EDS, identifying potential therapeutic targets, and providing opportunities for preclinical interventions. These models show similarities to their human counterparts in terms of clinical hallmarks, biomechanics, and tissue structure.
FRONTIERS IN GENETICS
(2021)
Article
Geriatrics & Gerontology
Matthew J. Wood, Rachel E. Miller, Anne-Marie Malfait
Summary: Approximately 20% of adults in the United States suffer from chronic pain, with the musculoskeletal system being one of the main sources, specifically osteoarthritis as the leading cause.
CLINICS IN GERIATRIC MEDICINE
(2022)
Article
Multidisciplinary Sciences
Alia M. Obeidat, Matthew J. Wood, Natalie S. Adamczyk, Shingo Ishihara, Jun Li, Lai Wang, Dongjun Ren, David A. Bennett, Richard J. Miller, Anne-Marie Malfait, Rachel E. Miller
Summary: Non-opioid targets are needed for addressing mechanical osteoarthritis pain. This study showed that nerve growth factor-mediated sensitization of joint nociceptors, critical for osteoarthritic pain, is dependent on Piezo2. Knocking out Piezo2 protected mice from various forms of joint pain.
NATURE COMMUNICATIONS
(2023)
Article
Rheumatology
Terese Geraghty, Alia M. Obeidat, Shingo Ishihara, Matthew J. Wood, Jun Li, Erika Barboza Prado Lopes, Carla R. Scanzello, Timothy M. Griffin, Anne-Marie Malfait, Rachel E. Miller
Summary: This study investigates the relationship between age and knee osteoarthritis (OA) and OA-related pain in mice. The findings show that aging in mice is associated with cartilage degeneration, pain-related behaviors, and changes in immune cell populations in the dorsal root ganglion. These findings provide potential new avenues for the development of OA therapies.
ARTHRITIS & RHEUMATOLOGY
(2023)
Article
Rheumatology
Lai Wang, Shingo Ishihara, Jun Li, Rachel E. Miller, Anne-Marie Malfait
Summary: This study aimed to explore the activation of the Notch signaling pathway in knee-innervating lumbar dorsal root ganglia (DRG) during experimental osteoarthritis (OA) in mice, and its role in knee hyperalgesia. The findings suggest that Notch signaling synergizes with TLR4 to promote CCL2 production and mediate knee hyperalgesia. Therefore, this pathway may be targeted for alleviating OA pain.
ARTHRITIS RESEARCH & THERAPY
(2023)
Article
Rheumatology
Shingo Ishihara, Alia M. Obeidat, David L. Wokosin, Dongjun Ren, Richard J. Miller, Anne-Marie Malfait, Rachel E. Miller
Summary: The study showed that CCL2-CCR2 signaling within the joint contributes to knee hyperalgesia in experimental osteoarthritis, partly mediated through direct stimulation of CCR2 expressed by intra-articular sensory afferents.
ARTHRITIS RESEARCH & THERAPY
(2021)