Article
Biochemistry & Molecular Biology
Ali Doryab, Otmar Schmid
Summary: Chronic lung diseases are a leading cause of death worldwide, and lung transplantation is currently the only treatment option but limited by donor availability. Lung tissue engineering offers a promising approach to regenerate damaged lung tissue. Researchers have developed an extracellular matrix-integrated scaffold that closely mimics the microenvironment of lung cells and shows potential for lung tissue engineering applications.
Article
Biotechnology & Applied Microbiology
Yuanyuan Shi, Yingxin Wang, Zhaochen Shan, Zhenhua Gao
Summary: Decellularized submandibular gland extracellular matrix (DSMG) was found to have similar structure and composition to dental pulp. DSMG could serve as an alternative scaffold for dental pulp regeneration, supporting adhesion and proliferation of dental pulp stem cells. Implanted cell-seeded DSMG formed a vascularized dental pulp-like tissue expressing markers involved in dentinogenesis and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Eun young Park, Jin hee Park, Nhu Thi Quynh Mai, Byoung-San Moon, Jung Kyu Choi
Summary: This study aimed to derive mature oocytes from murine preantral follicles cultured in a biomimetic ovary with a porcine scaffold using decellularization technology. The DNA content in the decell ovarian tissues was significantly lower than that in native tissues, but the integrity of the extracellular matrix (ECM) components was maintained. Hydrogels derived from decell porcine ovaries showed a complex fiber network and porous structure. Preantral follicles cultured in a biomimetic ovary with a decell porcine scaffold showed higher rates of antral follicle and mature oocytes development compared to other biomaterials.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Physical
Yolik Ramirez-Marin, David Eduardo Abad-Contreras, Martha Ustarroz-Cano, Norma S. Perez-Gallardo, Lorena Villafuerte-Garcia, Dulce Maria Puente-Guzman, Jorge Luna del Villar-Velasco, Leonardo Alejandro Rodriguez-Lopez, Gonzalo Torres-Villalobos, Miguel Angel Mercado, Jesus Tapia-Jurado, Francisco Drusso Martinez-Garcia, Martin Conrad Harmsen, M. Cristina Pina-Barba, David M. Giraldo-Gomez
Summary: Reconstruction of damaged bile ducts remains a challenging medical problem, and this study presents a method to obtain an extracellular matrix scaffold from porcine extrahepatic bile ducts. Following a standardized decellularization process, the scaffold showed no cytotoxicity, adequate cytocompatibility, and preserved hierarchical ultrastructure.
Article
Engineering, Multidisciplinary
Te-An Chen, Dhavan Sharma, Wenkai Jia, Donggi Ha, Kun Man, Jianhua Zhang, Yong Yang, Yuxiao Zhou, Timothy J. Kamp, Feng Zhao
Summary: Cell-derived extracellular matrix (ECM) is increasingly used in tissue engineering due to its ability to provide tailored signals for cellular responses. This study compared two detergent-based decellularization methods and found that EDTA + SDS treatment was more efficient in preserving the structure and bioactive substances of the ECM scaffold.
Article
Engineering, Biomedical
Yi Li, Qiong Wu, Li Li, Fei Chen, Ji Bao, Weimin Li
Summary: Utilizing a cocktail of dextrose, SLES, and Triton X-100 proved to be the most effective method for decellularizing porcine whole lung, preserving ECM composition while minimizing residual cellular components and xenoantigens. Evaluation through staining and immunofluorescence confirmed the noticeable preservation of ECM ultrastructure in the SLES-treated groups. The developed protocol holds promise for generating clinical-scale bioengineered lung scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Materials Science, Biomaterials
Min-Ah Koo, HaKyeong Jeong, Seung Hee Hong, Gyeung Mi Seon, Mi Hee Lee, Jong-Chul Park
Summary: This study successfully achieved complete decellularization of human dermal tissue through a combination of chemical and physical methods, while preserving its structural and functional characteristics. This method can be used to fabricate bioscaffolds with complex structures.
REGENERATIVE BIOMATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Aylin Acun, Ruben Oganesyan, Korkut Uygun, Heidi Yeh, Martin L. Yarmush, Basak E. Uygun
Summary: The extracellular matrix of the liver changes with age, affecting cell function, and donor age should be considered an important factor for bioengineering liver substitutes.
Article
Pathology
Shumei Mineta, Shunji Endo, Tomio Ueno
Summary: This study characterized the extraction and decellularization of human small intestinal submucosa. The results showed that the method by Abraham was most successful in achieving efficient tissue decellularization and viable cell survival and proliferation. It also met the criteria for residual DNA and growth factors in decellularized tissue.
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2023)
Article
Engineering, Biomedical
Evan T. Hoffman, Franziska E. Uhl, Loredana Asarian, Bin Deng, Chloe Becker, Juan J. Uriarte, Isaac Downs, Brad Young, Daniel J. Weiss
Summary: Chronic lung diseases, such as COPD and IPF, exhibit region-specific changes in extracellular matrix (ECM) composition, particularly in collagen and basement-membrane associated proteins. This study compares the ECM composition of whole decellularized lungs and specific anatomical lung regions in non-diseased, COPD, and IPF human lungs. The findings demonstrate distinct ECM signatures in different regions and reveal alterations in ECM composition specific to COPD and IPF, including enrichment of type-III collagen and fibulin in IPF aECM. This study provides methodology for future research and a dataset for identifying novel ECM targets for therapeutics.
Article
Engineering, Biomedical
Maryam Khajavi, Abdolmajid Hajimoradloo, Mojgan Zandi, Mohamad Pezeshki-Modaress, Shahin Bonakdar, Abbas Zamani
Summary: The study successfully engineered a cartilage-like scaffold using ECM from sturgeon fish cartilage to stimulate cartilaginous matrix synthesis in human adipose stem cells. The porous scaffold derived from fish cartilage ECM showed excellent potential for cartilage tissue engineering, supporting cell adhesion, proliferation, and chondrogenic differentiation. The scaffold was demonstrated to facilitate the synthesis of specific cartilage proteins, such as collagen type II and aggrecan, making it a promising option for tissue regeneration.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Nanoscience & Nanotechnology
My Thi Ngoc Nguyen, Ha Le Bao Tran
Summary: The study reported the heparinization of bovine pericardial scaffolds using the Layer-by-Layer self-assembly technique, which significantly improved the anticoagulant properties of the scaffolds, showing potential applications in the cardiovascular field.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
Article
Chemistry, Multidisciplinary
Alireza Shahin-Shamsabadi, John Cappuccitti
Summary: Biofabricated in vitro models have the potential to accelerate drug discovery and reduce new drug costs, but current models are oversimplified. An innovative platform is introduced using 2D culture systems and scaffold-free cell sheets to create self-assembling 3D tissue constructs, providing more physiological relevance and precise control over cellular behavior.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Huimin Xiao, Xin Chen, Xuanzhe Liu, Gen Wen, Yaling Yu
Summary: The skin is an important organ that protects the body and interacts with the external environment. Decellularized biomaterials derived from the extracellular matrix have shown potential in promoting wound healing and skin regeneration. This article reviews the wound healing process, the role of extracellular matrix constituents in wound healing, and the various types of decellularized materials used in clinical practice. The challenges and future research directions in utilizing decellularized biomaterials for wound treatment are also discussed.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Lijun Zhang, Qi Xing, Zichen Qian, Mitchell Tahtinen, Zhaoqiang Zhang, Emily Shearier, Shaohai Qi, Feng Zhao
ADVANCED HEALTHCARE MATERIALS
(2016)
Article
Cell & Tissue Engineering
Emily Shearier, Qi Xing, Zichen Qian, Feng Zhao
TISSUE ENGINEERING PART C-METHODS
(2016)
Article
Chemistry, Multidisciplinary
Emily Shearier, Peifu Cheng, Zhuan Zhu, Jiming Bao, Yun Hang Hu, Feng Zhao
Review
Engineering, Biomedical
Patrick K. Bowen, Emily R. Shearier, Shan Zhao, Roger J. Guillory, Feng Zhao, Jeremy Goldman, Jaroslaw W. Drelich
ADVANCED HEALTHCARE MATERIALS
(2016)
Article
Materials Science, Biomaterials
Emily R. Shearier, Patrick K. Bowen, Weilue He, Adam Drench, Jaroslaw Drelich, Jeremy Goldman, Feng Zhao
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2016)
Article
Materials Science, Biomaterials
Roger J. Guillory, Patrick K. Bowen, Sean P. Hopkins, Emily R. Shearier, Elisha J. Earley, Amani A. Gillette, Eli Aghion, Martin Bocks, Jaroslaw W. Drelich, Jeremy Goldman
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2016)
Review
Materials Science, Biomaterials
Qi Xing, Zichen Qian, Wenkai Jia, Avik Ghosh, Mitchell Tahtinen, Feng Zhao
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Materials Science, Biomaterials
Emily R. Shearier, Patrick K. Bowen, Weilue He, Adam Drench, Jaroslaw Drelich, Jeremy Goldman, Feng Zhao
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2016)
Review
Materials Science, Biomaterials
Qi Xing, Zichen Qian, Wenkai Jia, Avik Ghosh, Mitchell Tahtinen, Feng Zhao
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Medicine, Research & Experimental
Lei Chen, Qi Xing, Qiyi Zhai, Mitchell Tahtinen, Fei Zhou, Lili Chen, Yingbin Xu, Shaohai Qi, Feng Zhao
Article
Engineering, Biomedical
Qi Xing, Zichen Qian, Mitchell Tahtinen, Ai Hui Yap, Keegan Yates, Feng Zhao
ADVANCED HEALTHCARE MATERIALS
(2017)
Article
Pediatrics
Charan Kumar Devarakonda, Emily R. Shearier, Chaoran Hu, James Grady, Jeremy L. Balsbaugh, John H. Makari, Fernando A. Ferrer, Linda H. Shapiro
JOURNAL OF PEDIATRIC UROLOGY
(2020)
Article
Cell & Tissue Engineering
Wenkai Jia, Dhavan Sharma, Weilue He, Qi Xing, Feng Zhao
Summary: The study aimed to investigate the effects of differentiation cues on the microvascular structure, angiogenic factor secretion, and immunogenic responses of prevascularized hMSC sheets. Results showed that coculturing with ECs enabled hMSC sheets to form a microvascular network while maintaining their multi-lineage differentiation capability. Osteogenic and adipogenic induction damaged the preformed microvascular structures and compromised the angiogenic factor secretion ability of hMSCs, although this effect could be mitigated by adjusting the concentration of differentiation factors. Subcutaneous transplantation in an immunocompetent rat model demonstrated that osteogenic differentiated prevascularized hMSC sheets preserved their microvascular structure and immunomodulatory properties comparable to undifferentiated prevascularized hMSC sheets.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Zichen Qian, David Ross, Wenkai Jia, Qi Xing, Feng Zhao
BIOACTIVE MATERIALS
(2018)