Article
Biochemistry & Molecular Biology
Takeshi Sugimoto, Tomohiro Nakamura, Sho Yokoyama, Toshia Fujisato, Satoshi Konishi, Takeshi Hashimoto
Summary: This study investigated the effects of electrical pulse stimulation (EPS)-evoked muscle contraction on irisin and CTSB secretion in 3D-engineered muscle (3D-EM). The results showed a significant increase in irisin secretion following EPS, while no significant difference was observed in CTSB secretion between the two groups. Irisin may be a contractile muscle-derived myokine, but CTSB is not secreted by EPS-evoked muscle contractile stimulation in 3D-EM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dongyun Kim, Hanjun Hwangbo, GeunHyung Kim
Summary: By utilizing a submerged bioprinting process, aligned cell-laden filaments with high viability and gene expression levels were successfully fabricated, showing potential for obtaining bioengineered constructs for various tissues.
Article
Critical Care Medicine
Debora R. Campos, Thatiana B. C. Bueno, Jackeline S. G. G. Anjos, Daniel Zoppi, Bruno G. Dantas, Rik Gosselink, Rinaldo R. J. Guirro, Marcos C. Borges
Summary: This study evaluated the impact of additional use of early neuromuscular electrical stimulation (NMES) on an early mobilization (EM) protocol. The results showed that patients in the EM+NMES group had better functional status outcomes on the first day awake and at ICU and hospital discharge. They also took fewer days to stand up, had shorter hospital length of stay, lower frequency of ICU-acquired weakness, and better muscle strength.
CRITICAL CARE MEDICINE
(2022)
Article
Cell Biology
Jacqueline M. Bliley, Mathilde C. S. C. Vermeer, Rebecca M. Duffy, Ivan Batalov, Duco Kramer, Joshua W. Tashman, Daniel J. Shiwarski, Andrew Lee, Alexander S. Teplenin, Linda Volkers, Brian Coffin, Martijn F. Hoes, Anna Kalmykov, Rachelle N. Palchesko, Yan Sun, Jan D. H. Jongbloed, Nils Bomer, Rudolf A. de Boer, Albert J. H. Suurmeijer, Daniel A. Pijnappels, Maria C. Bolling, Peter van der Meer, Adam W. Feinberg
Summary: The role of mechanical forces in shaping the heart is crucial but challenging to study in patients. A new dynamic EHT model was developed, allowing for improved function of wild-type EHTs with dynamic loading. Disease modeling using this dynamic loading strategy was essential for inducing specific disease phenotypes.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Physiology
Thierry Paillard
Summary: Percutaneous electrical stimulation is a method used to improve functional capabilities in older individuals. The specific physiological needs of the individual determine whether neuromuscular electrical stimulation (NMES) or sensory electrical stimulation (SES) is most appropriate. NMES enhances muscle strength and motor output, while SES facilitates sensorimotor activity.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Clinical Neurology
Annabella Kurz, Gerd Fabian Volk, Dirk Arnold, Berit Schneider-Stickler, Winfried Mayr, Orlando Guntinas-Lichius
Summary: This article discusses the potential clinical value of surface electrical stimulation in the acute phase of denervation after facial nerve or recurrent laryngeal nerve paralysis, highlighting similarities between the two nerve lesions and the importance of directly stimulating denervated muscles. Early electromuscular stimulation may improve functional outcomes, but further research is needed to confirm its efficacy in larger controlled trials.
FRONTIERS IN NEUROLOGY
(2022)
Article
Engineering, Biomedical
Gongkai Ye, Pirashanth Theventhiran, Kei Masani
Summary: This study investigated the fatigue reducing ability of spatially distributed sequential stimulation (SDSS) in functional electrical stimulation (FES)-rowing. The results showed that SDSS significantly reduced fatigue and lengthened the rowing time by approximately 30%. The study demonstrated that the application of SDSS could enhance the effectiveness of FES-rowing as a rehabilitative exercise.
IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING
(2022)
Article
Engineering, Biomedical
Nethika R. Ariyasinghe, Jeffrey W. Santoso, Divya Gupta, Mark J. Pincus, Paul R. August, Megan L. McCain
Summary: Recent developments in muscle tissue engineering include rapid prototyping of 3D muscle bundle templates, engineering techniques, and imaging methods to overcome limitations of existing models systems. Tissue clearing may cause some tissue degradation.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Erin Rousseau, Ritu Raman, Tigist Tamir, Angel Bu, Shriya Srinivasan, Naomi Lynch, Robert Langer, Forest M. White, Michael J. Cima
Summary: Damage to skeletal muscle tissue can have a severe impact on health and mobility. The study shows that targeted activation of implanted muscle grafts through non-invasive light stimulation restores motor function to levels similar to healthy mice. Analysis also reveals that repeated muscle contraction alters signaling pathways involved in muscle contractility, injury adaptation, neurite growth, neuromuscular synapse formation, angiogenesis, and cytoskeletal remodeling.
Article
Engineering, Biomedical
Bingchuan Lu, Min Ye, Jingjing Xia, Zhenrui Zhang, Zhuo Xiong, Ting Zhang
Summary: The formation of multiscale vascular networks is essential for the construction of large-scale biomimetic cardiac tissues/organs in vitro. Electrical stimulation is applied to promote the elongation and connection of endothelial cells in engineered cardiac tissue (ECT) and achieve vascularization. The results show that electrical stimulation significantly enhances the elongation, migration, and interconnection of endothelial cells in ECT and increases the expression of related genes. Electrical stimulation also enhances the secretion of signal factors between cardiomyocytes and endothelial cells and contributes to the contractility of cardiac tissue.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Tyler Johnson, Dawn Taylor
Summary: This study investigates how modulating cocontraction of antagonist muscles during functional electrical stimulation (FES) can impact reaching performance and energy usage. The results suggest that dynamically modulating limb stiffness can significantly improve energy usage and movement performance. Therefore, brain-controlled FES becomes a more viable option for restoration of reaching after paralysis.
JOURNAL OF NEURAL ENGINEERING
(2021)
Review
Chemistry, Analytical
Thomas Aout, Mickael Begon, Baptiste Jegou, Nicolas Peyrot, Teddy Caderby
Summary: This systematic review examines the effects of functional electrical stimulation (FES) on gait characteristics in healthy individuals. The review identified 15 studies and found that FES can modify lower-limb joint kinematics and kinetics parameters, resulting in changes in spatiotemporal gait parameters.
Article
Biotechnology & Applied Microbiology
Tomohiro Nakamura, Shunya Takagi, Daisuke Okuzaki, Seika Matsui, Toshia Fujisato
Summary: High altitude environments can lead to a decrease in oxygen partial pressure in the body, causing metabolic changes and anorectic effects. Experimental results suggest that the elevation in plasma CCK levels following acute exercise or at high altitude may be partly attributed to myogenic cells.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Chemistry, Analytical
Qiang Zhang, Ashwin Iyer, Krysten Lambeth, Kang Kim, Nitin Sharma
Summary: Functional electrical stimulation (FES) is a potential neurorehabilitative intervention for enabling functional movements in individuals with mobility impairments. This study proposes the use of ultrasound imaging-derived echogenicity signal as an indicator of FES-induced muscle fatigue. The experimental results show a strong relationship between the ultrasound-derived signal and muscle fatigue under isometric and dynamic muscle contraction conditions.
Article
Geriatrics & Gerontology
Le Li, Chengpeng Hu, Kenry W. C. Leung, Raymond K. Y. Tong
Summary: This study aimed to investigate the immediate electrical impedance property changes in the lower extremity muscles of chronic stroke survivors after functional electrical stimulation (FES)-assisted cycling training. The results showed that after training, the reactance and phase angle values of the muscles significantly increased, and there was a significant correlation with clinical assessment scales. These findings contribute to understanding the mechanism of electrical stimulation-assisted exercise in improving muscle function in stroke survivors.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Editorial Material
Pharmacology & Pharmacy
Torie Broer, Alastair Khodabukus, Nenad Bursac
EXPERT OPINION ON DRUG DISCOVERY
(2020)
Article
Cell & Tissue Engineering
Megan E. Kondash, Anandita Ananthakumar, Alastair Khodabukus, Nenad Bursac, George A. Truskey
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Toxicology
Alastair Khodabukus, Amulya Kaza, Jason Wang, Neel Prabhu, Richard Goldstein, Vishal S. Vaidya, Nenad Bursac
TOXICOLOGICAL SCIENCES
(2020)
Article
Geriatrics & Gerontology
Henning T. Langer, Agata A. Mossakowski, Brandon J. Willis, Kristin N. Grimsrud, Joshua A. Wood, Kevin C. K. Lloyd, Hermann Zbinden-Foncea, Keith Baar
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2020)
Review
Physiology
Alastair Khodabukus
Summary: Skeletal muscle is highly plastic and small animal models have been important in understanding adaptation and plasticity, although they do not accurately model human muscle diseases. In vitro three-dimensional tissue-engineered skeletal muscle models show potential in studying muscle function, plasticity, and disease, including patient-specific modeling of diseases like Duchenne muscular dystrophy (DMD) and volumetric muscle loss. Further development is needed for these models to become more mature, biomimetic, and widely used for studying muscle physiology, disease, and clinical applications.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Jason Wang, Chris J. Zhou, Alastair Khodabukus, Sabrina Tran, Sang-Oh Han, Aaron L. Carlson, Lauran Madden, Priya S. Kishnani, Dwight D. Koeberl, Nenad Bursac
Summary: The study developed a 3D in vitro model of human skeletal muscle that recapitulates the pathophysiology of Pompe disease. They identified a Pompe disease-specific transcriptional signature and observed a partial reversal of the signature upon in vitro treatment of myobundles with rhGAA.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Alec M. Avey, Keith Baar
Summary: In organisms, the initial formation of a functional tendon relies on chemical signals from muscles, and further study is needed on how myokines affect tendon maturation, maintenance, and regeneration with age. An experimental framework is proposed to investigate the muscle-tendon crosstalk, aiming to develop new interventions for tendon development and function improvement.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Cell Biology
Zachary Fralish, Ethan M. Lotz, Taylor Chavez, Alastair Khodabukus, Nenad Bursac
Summary: The neuromuscular junction (NMJ) is a crucial interface between motor neurons and skeletal muscle fibers that is essential for motor function. This review discusses the development of in vitro models of human NMJs derived from induced pluripotent stem cells (hiPSCs) and compares the efficacy of modeling neuromuscular diseases (NMDs) in animals and cell culture systems. Further research is needed to develop effective personalized NMD platforms using hiPSC-derived NMJ models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Jason Wang, Torie Broer, Taylor Chavez, Chris J. Zhou, Sabrina Tran, Yu Xiang, Alastair Khodabukus, Yarui Diao, Nenad Bursac
Summary: This study optimized the methods for deactivating in vitro expanded human myoblasts within a 3D culture of engineered human skeletal muscle tissues. The results showed that a fraction of myoblasts within the culture adopted a quiescent phenotype similar to native satellite cells, and could be reactivated. Single cell RNA-sequencing revealed the existence of two subpopulations of these quiescent and activated cells, and characterized their gene expression changes. This study provides a platform for further research on human muscle regeneration and disease-associated satellite cell dysfunction.
Article
Biochemistry & Molecular Biology
Danielle Steffen, Michael J. Mienaltowski, Keith Baar
Summary: The effect of mechanical load on tendinopathic tissue varies depending on the type of load. Dynamic loading increases fibrocartilage markers, while long isometric loads stimulate markers of tendon regeneration.
Article
Cell Biology
Suraj J. Pathak, Zeyu Zhou, Danielle Steffen, Tommy Tran, Yael Ad, Jon J. Ramsey, Jennifer M. Rutkowsky, Keith Baar
Summary: A 2-month isocaloric ketogenic diet in middle-aged female mice has been found to enhance cognitive behavior, including anxiety, memory, and willingness to explore. This improvement may be associated with the interaction between muscle and brain.
Review
Cell Biology
Alastair Khodabukus, Tyler Guyer, Axel C. Moore, Molly M. Stevens, Robert E. Guldberg, Nenad Bursac
Summary: Musculoskeletal injuries and disorders are the main cause of physical disability globally, posing a significant socioeconomic burden. The lack of effective treatments has led to the development of novel bioengineering approaches. This review discusses the self-repair capacity of musculoskeletal tissues, the causes of musculoskeletal dysfunction, and the advancements in biomaterial, immunomodulatory, cellular, and gene therapies for treating musculoskeletal disorders.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Physiology
Christopher G. Vann, Xin Zhang, Alastair Khodabukus, Melissa C. C. Orenduff, Yu-Hsiu Chen, David L. L. Corcoran, George A. A. Truskey, Nenad Bursac, Virginia B. B. Kraus
Summary: Exercise-mimetic contractile activity of human engineered muscle affects the expression of miRs and the number of secreted EVs, providing new insights into the role of exercise in organ-organ interactions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
David E. Lee, Lauren K. McKay, Akshay Bareja, Yongwu Li, Alastair Khodabukus, Nenad Bursac, Gregory A. Taylor, Gurpreet S. Baht, James P. White
Summary: Pathologies associated with sarcopenia include decline in muscular strength, lean mass and regenerative capacity. This study investigates the therapeutic potential of METRNL to improve aged muscle through improving muscle regeneration and countering age-related loss in muscle resilience by triggering apoptosis of fibro/adipogenic progenitor cells.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Danielle Steffen, Alec Avey, Michael J. J. Mienaltowski, Keith Baar
Summary: This study investigated the morphological, mechanical, and transcriptional changes in the rat Achilles and patellar tendon during post-natal development. The tendons showed similar increases in collagen content and mechanical strength. However, the patellar tendon tended to have a higher tensile load at maturity, possibly due to its increased cross-sectional area. Transcriptional analysis revealed that the tendons became distinct at maturity, with the patellar tendon showing lower STAT signaling. Treating engineered human ligaments with a STAT inhibitor increased collagen content and tensile load. These findings suggest that post-natal tendon development involves a shift towards extracellular matrix development and provide potential targets for improving tendon function.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)