Article
Cell & Tissue Engineering
Jean-Marie Jacob, Selene E. Di Carlo, Igor Stzepourginski, Anthony Lepelletier, Papa Diogop Ndiaye, Hugo Varet, Rachel Legendre, Etienne Kornobis, Adam Benabid, Giulia Nigro, Lucie Peduto
Summary: The transcriptomic switch induced by PDGFR alpha in intestinal stromal cells is crucial for coordinating postnatal intestinal maturation and function.
Review
Biochemistry & Molecular Biology
Huda I. Atiya, Grace Gorecki, Geyon L. Garcia, Leonard G. Frisbie, Roja Baruwal, Lan Coffman
Summary: The ability of cancer cells to detach from the primary site and metastasize is the main cause of cancer-related death. The first event in the metastatic cascade is epithelial-to-mesenchymal transition (EMT), which leads to the loss of cell-cell adhesion and the acquisition of motile and stem-like phenotypes. The tumor microenvironment (TME) plays a critical role in modulating EMT in cancer cells, promoting the acquisition of a mesenchymal phenotype through interaction or changes with cancer cells.
Article
Engineering, Biomedical
Adrian Cervero-Varona, Angelo Canciello, Alessia Peserico, Arlette Alina Haidar Montes, Maria Rita Citeroni, Annunziata Mauro, Valentina Russo, Samanta Moffa, Serena Pilato, Stefano Di Giacomo, Beatrice Dufrusine, Enrico Dainese, Antonella Fontana, Barbara Barboni
Summary: The use of biomaterials in immune regenerative strategies for unresolved pathologies is gaining attention in tissue engineering. Graphene oxide (GO) has been proposed as an immune-mimetic material for stem cell-based regenerative therapies, influencing stem cell behavior and immune response. Amniotic epithelial stem cells (AECs) are also of interest due to their plasticity and immunomodulatory paracrine activities, and the effects of GO on AECs are explored in this study. GO-coating induced and accelerated the Epithelial-Mesenchymal Transition (EMT) in AECs, leading to changes in cytokine secretion and enhanced activation of macrophages. Adhesion of AECs on GO-functionalized surfaces could create a tailored microenvironment for the regenerative process.
MATERIALS TODAY BIO
(2023)
Article
Oncology
Zhiwei Liang, Jian Li, Longshuan Zhao, Yilei Deng
Summary: miR-375 is significantly downregulated in liver fibrosis, and it regulates hepatic stellate cell (HSC) activity and epithelial-mesenchymal transition (EMT) via RAC1 to exert its anti-liver fibrosis function. This study identified the miR-375/RAC1 axis as a novel regulatory mechanism in the development of liver fibrosis.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Seung Kak Shin, Sujin Ryu, Seungyoon Nam, Seung Yeon Ha, Oh Sang Kwon, Yun Soo Kim, Se-Hee Kim, Ju Hyun Kim
Summary: This study evaluated the correlations between clinicopathological characteristics and epithelial-mesenchymal transition (EMT) markers in hepatocellular carcinoma (HCC) patients who underwent surgical resection, and identified a key regulator in the EMT process. The down-expression of E-cadherin and overexpression of p21-activated kinases 1 (PAK1)/Snail in HCC tissues were significantly associated with macrovascular invasion, microvascular invasion, large tumor size, and advanced tumor stage. Patients with these EMT markers showed early recurrence and poor overall survival. In vitro studies showed that inhibition of Rac1 decreased the expression of EMT markers and suppressed the migration and invasion of HCC cells. Therefore, Rac1 may be a potential therapeutic target for inhibiting EMT process in HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Xue Yang, Qing Li, Wenting Liu, Chen Zong, Lixin Wei, Yufang Shi, Zhipeng Han
Summary: Hepatic fibrosis/cirrhosis is a complex condition with a significant global health burden. Endogenous and exogenous mesenchymal stromal cells (MSCs) play key roles in its pathogenesis and treatment. Clinical trials have shown the effectiveness of exogenous MSCs in liver cirrhosis, but there are still challenges to be addressed for their future clinical use. This review examines the role of MSCs in hepatic fibrosis/cirrhosis and discusses the standardization of parameters and promotion of MSCs' clinical application.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Agostina M. Onorato, Esteban Fiore, Juan Bayo, Cecilia Casali, Maria Fernandez-Tome, Marcelo Rodriguez, Luciana Dominguez, Josepmaria Argemi, Florencia Hidalgo, Cristian Favre, Mariana Garcia, Catalina Atorrasagasti, Guillermo D. Mazzolini
Summary: The absence of SPARC leads to altered hepatic lipid metabolism and accelerates the development of NAFLD-related HCC.
LIVER INTERNATIONAL
(2021)
Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Gastroenterology & Hepatology
Zhilin Sha, Yajie Yang, Ruling Liu, Haili Bao, Shaohua Song, Junfeng Dong, Meng Guo, Yuanyu Zhao, Hu Liu, Guoshan Ding
Summary: This study demonstrates the protective effect of Rac1 inhibition on hepatic IRI by reducing ROS production and oxidative stress, and uncovers an interaction between Rac1 and HIF-1 alpha signaling pathways during hepatic IRI.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2022)
Article
Environmental Sciences
Hana Najahi, Nicola Alessio, Tiziana Squillaro, Gea Oliveri Conti, Margherita Ferrante, Giovanni Di Bernardo, Umberto Galderisi, Imed Messaoudi, Sergio Minucci, Mohamed Banni
Summary: This study evaluated the effects of different size microplastics present in water bottles on human mesenchymal stromal cells. The results showed that microplastic treatments led to a decrease in cell proliferation, altered differentiation potential, and loss of osteocyte phenotype. This indicates that environmental microplastics can be bioavailable for cell uptake and may result in irreversible diseases.
ENVIRONMENTAL RESEARCH
(2022)
Article
Oncology
Subbulakshmi Karthikeyan, Patrick J. Casey, Mei Wang
Summary: This study identifies the RAB4A-RAC1 signaling axis as a key regulatory mechanism for EMT and cancer progression, suggesting a potential therapeutic approach to controlling these processes.
BREAST CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yuka Sasaki, Ryusuke Nakatsuka, Takuma Inouchi, Mitsuko Masutani, Tadashige Nozaki
Summary: Inhibition of PARG protein activity accelerates osteoblast differentiation in MC3T3-E1 cells, providing potential therapeutic benefits for metabolic bone diseases such as osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Srinivasa R. Sripathi, Ming-Wen Hu, Ravi Chakra Turaga, Rebekah Mikeasky, Ganesh Satyanarayana, Jie Cheng, Yukan Duan, Julien Maruotti, Karl J. Wahlin, Cynthia A. Berlinicke, Jiang Qian, Noriko Esumi, Donald J. Zack
Summary: Epithelial-mesenchymal transition (EMT) plays an important role in embryonic development, malignant transformation, and tumor progression, and is also implicated in various retinal diseases. The molecular mechanisms underlying EMT of the retinal pigment epithelium (RPE) are not fully understood. In this study, a small molecule inhibitor, BAY651942, was found to modulate TGF-beta/TNF-alpha-induced RPE-EMT. RNA-seq analysis revealed altered biological pathways and signaling events in BAY651942-treated hRPE monolayers. Inhibition of IKK beta restores RPE identity and may offer a promising approach for treating retinal diseases involving RPE dedifferentiation and EMT.
Article
Cell & Tissue Engineering
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: The study identifies HSPB7 as a regulator of osteogenic and adipogenic differentiation in bone marrow-derived mesenchymal stromal cells (BMSCs), with a positive role in osteoblastic differentiation and maintaining the balance between osteogenesis and adipogenesis. HSPB7 shows potential as a therapeutic target for bone metabolic diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Wen-Ting Hsieh, Yi-Shiuan Liu, Yi-hsuan Lee, Marilyn G. Rimando, Keng-hui Lin, Oscar K. Lee
ACTA BIOMATERIALIA
(2016)
Article
Multidisciplinary Sciences
Yin-Quan Chen, Yi-Shiuan Liu, Yu-An Liu, Yi-Chang Wu, Juan C. del Alamo, Arthur Chiou, Oscar K. Lee
SCIENTIFIC REPORTS
(2016)
Article
Cell & Tissue Engineering
Meng-Hua Yen, Yuan-Yi Wu, Yi-Shiuan Liu, Marilyn Rimando, Jennifer Hui-Chun Ho, Oscar Kuang-Sheng Lee
STEM CELL RESEARCH & THERAPY
(2016)
Article
Biochemistry & Molecular Biology
Shu-Wen Kuo, Marilyn G. Rimando, Yi-Shiuan Liu, Oscar K. Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Yu-Tzu Tsao, Yi-Jeng Huang, Hao-Hsiang Wu, Yu-An Liu, Yi-Shiuan Liu, Oscar K. Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Cell & Tissue Engineering
Yu-Tzu Tsao, Ya-Yi Shih, Yu-An Liu, Yi-Shiuan Liu, Oscar K. Lee
STEM CELL RESEARCH & THERAPY
(2017)
Article
Multidisciplinary Sciences
Yin-Ping Lo, Yi-Shiuan Liu, Marilyn G. Rimando, Jennifer Hui-Chun Ho, Keng-hui Lin, Oscar K. Lee
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Marilyn G. Rimando, Hao-Hsiang Wu, Yu-An Liu, Chien-Wei Lee, Shu-Wen Kuo, Yin-Ping Lo, Kuo-Fung Tseng, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Meng-Hua Yen, Yu-Han Chen, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Yi-Shiuan Liu, Chih-Yu Yang, Ping-Fang Chiu, Hui-Chu Lin, Chung-Chuan Lo, Alan Szu-Han Lai, Chia-Chu Chang, Oscar Kuang-Sheng Lee
Summary: The study aimed to develop machine learning algorithms to predict intradialytic adverse events during hemodialysis. By collecting dialysis and physiological time-series data, extracting features through linear and differential analyses, building models, and cross-validating them, the results showed that the algorithm achieved high AUC in quasi-real time prediction of adverse events during hemodialysis.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Ching-Hui Chien, Yi-Shiuan Liu, Yen-Ting Chang, Yunn-Hwa Ma
Summary: The study demonstrates that Epigallocatechin gallate (EGCG) significantly enhances the uptake of magnetic nanoparticles (MNPs) by mesenchymal stem cells (MSCs). After treatment with EGCG in a magnetic field for 2 hours, the cell-associated MNPs increased 7.1 and 3.4 times in mouse and human MSCs, respectively, without affecting cell morphology. Furthermore, the study shows that the stemness and differentiation capacity of MSCs are not affected by the MNP uptake.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2022)
Review
Physiology
Ting-Wei Kao, Yi-Shiuan Liu, Chih-Yu Yang, Oscar Kuang-Sheng Lee
Summary: This review discusses the role of mechanical input in the differentiation of mesenchymal stem cells (MSCs) and the underlying mechanisms. Optimal cultural parameters, such as spatial boundary conditions and types of forces, can influence MSCs commitment. The concept of mechanical memory is introduced to understand how MSCs store information from previous culture environments. The cardiovascular system and immune system may share similar mechanisms of mechanosensing and mechanotransduction. The review summarizes the current evidence and highlights future perspectives in cardiovascular bioengineering and regenerative medicine.
CHINESE JOURNAL OF PHYSIOLOGY
(2023)
Meeting Abstract
Behavioral Sciences
Sven-Eric Jordt, Michael A. Ha, J. Smith, Joseph A. Cichocki, Shrilatha Balakrishna, Lu Fan, Yi-Shiuan Liu, Ana I. Caceres, Marina R. Picciotto, John B. Morris