Article
Chemistry, Organic
Min Zhang, Dumei Ma, Jianxi Ying, Yufen Zhao
Summary: Pyrophosphates with P(O)-O-P(O) linkages are of interest in the field of biologically active substances. In this study, we developed a method utilizing acetate ion-assisted P-O-P bond formation using H-Phosphonates to obtain tetraalkyl pyrophosphates. NMR experiments revealed that the acetate ion plays a role in the formation of the P-O-P bond.
TETRAHEDRON LETTERS
(2023)
Article
Chemistry, Physical
Like Huang, Liu Wu, Qihao Sun, Chenxing Jin, Jiahao Wang, Shiqiang Fu, Zhangting Wu, Xiaohui Liu, Ziyang Hu, Jing Zhang, Jia Sun, Xiaojian Zhu, Yuejin Zhu
Summary: A multifunctional device based on halide perovskite p-n heterojunction diode is reported, enabling integration of photovoltaic, photodetection, and photosynaptic functions with high efficiency and stability.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Monica Martinez-Estevez, Soledad Garcia-Fontan, Saray Argibay-Otero, Inmaculada Prieto, Ezequiel M. Vazquez-Lopez
Summary: In this study, the reaction between [Ru2Cl2(mu-Cl)(2)(eta(6)-p-cymene)(2)] and two thiosemicarbazones was investigated. The resulting cationic complexes were isolated and characterized. The study revealed the presence of two isomers in the triflate salts and only one isomer in the chloride. X-ray crystallography confirmed this finding. The coordination of the thiosemicarbazone ligand to the ruthenium center was also demonstrated.
Article
Electrochemistry
Dmitry A. Dulov, Anna S. Rumyantseva, Konstantin A. Lyssenko, Ksenia A. Paseshnichenko, Yuri K. Grishin, Tatiana V. Magdesieva
Summary: The cathodic generation of the halogenating agent combined with the anodic oxidation of diarylamines provides an efficient and selective method for the direct conversion of 2,4'-disubstituted diarylamines to N,N'-diarylbenzidines with 5,5'-chlorination in the biphenyl fragment. The proposed protocol is applicable to starting compounds, yielding the chlorinated benzidines in good yields. Additionally, the method shows regioselectivity differences between CCl4 and CBr4, due to distinct reaction routes and steric reasons.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Gabriele Hierlmeier, Robert Wolf
Summary: The reactivity of di-tert-butyldiphosphatetrahedrane was studied, showing selective activation by N-heterocyclic carbene nickel(I) and nickel(0) complexes to form novel complexes. Furthermore, release of the tetrahedral framework was achieved by addition of CO gas.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Plant Sciences
Wendy J. Lyzenga, Zhigang Liu, Toluwase Olukayode, Yang Zhao, Leon V. Kochian, Byung-Kook Ham
Summary: Enhancing nutrient use efficiency in agriculture requires understanding the molecular features of nutrient uptake by roots, root system architecture, and crop nutrient use efficiency. The main source of essential mineral nutrients for plants is the soil, which plays a crucial role in plant biomolecule synthesis, cell processes, and enzymatic reactions. The roots serve as the main entry point for mineral nutrients, and various transport systems, sensors, and signaling proteins facilitate their uptake. Chemical fertilizers containing nitrogen, phosphorus, and potassium are commonly used to maximize crop yields. However, optimizing nutrient uptake and utilization is essential for sustainable agriculture. Therefore, this review focuses on the molecular basis of N, P, and K nutrient uptake, the relationship between root system architecture and nutrient distribution, and the coordination of nutrient uptake with photosynthesis through the xylem and phloem. The size, structure, and activity of the root system, along with nutrient transporters, determine the rate of nutrient acquisition. The connections between N, P, and K uptake and signaling are also discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Multidisciplinary
Marie-Helene Tremblay, Kelly Schutt, Yadong Zhang, Stephen Barlow, Henry J. Snaith, Seth R. Marder
Summary: A newly developed norbornene homopolymer with hole-transporting side chains was compared to spiro-OMeTAD as a hole-transport material in perovskite solar cells. The study showed that PSCs fabricated with the polymer achieved a comparable efficiency to those with spiro-OMeTAD, while demonstrating improved light and heat stability.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Ai-Ran Liu, Lei Zhang, Jiao Li, Abudureheman Wusiman
Summary: A straightforward one-pot, multicomponent approach was developed to synthesize di- and tri-substituted N-sulfonyl formamidines from sulfonyl chlorides, NaN3, ethyl propiolate, and primary/secondary amines under mild conditions without catalysts or additives. Structural analysis showed the formation of E-syn/anti isomeric form for di-substituted sulfonyl formamidines, while tri-substituted analogues only formed E-isomers.
Article
Polymer Science
Simeng Xiang, Bin Tang, Jiao Feng, Xiang Lin, Feng Liu, Hongyu Yang, Xiaming Feng, Chaojun Wan
Summary: A supramolecular flame retardant (ATPFR) was facilely created using cheap and accessible raw materials in an ecologically benign aqueous solvent, and applied to improve the fire safety of epoxy resins (EP). With only 5 wt% ATPFR addition, EP achieved a limiting oxygen index of 28.5% and a UL-94 V-0 rating with a significant blow-out effect. EP composites with the small amount of ATPFR exhibited slight decrease in mechanical properties while maintaining good performance.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Philipp Royla, Kai Schwedtmann, Zeyu Han, Jannis Fidelius, Derek P. Gates, Rosa M. Gomila, Antonio Frontera, Jan J. Weigand
Summary: Cationic imidazoliumyl(phosphonio)-phosphanides were obtained via the nucleophilic fragmentation of tetracationic tetraphosphetane with tertiary phosphanes. They can act as [LC-P]+ transfer reagents in phospha-Wittig-type reactions, yielding cationic phosphaalkenes and phosphanides. The versatility of [LC-P]+ as a valuable P1 building block was demonstrated in substitution reactions, providing a convenient access to difficult-to-synthesize compounds.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Energy & Fuels
Jinhan Teng, Xin Tang, Manqin Tang, Qian Wu, Jing Li
Summary: The N/P ratio of a lithium-ion battery is crucial for stabilizing and adjusting battery performance. Research shows that a low N/P ratio is beneficial for high energy density battery design and use. Adjusting the cut-off voltage and negative electrode potential can effectively prevent lithium metal deposition, increasing battery life and capacity retention.
JOURNAL OF ENERGY STORAGE
(2022)
Article
Biochemistry & Molecular Biology
Atieh Rezvanian, Behnoosh Khodadadi, Sepideh Tafreshi, Pezhman Shiri
Summary: Here, highly efficient one-pot protocols for the synthesis of structurally diverse fused N-containing heterocycles containing 2-chloroquinoline have been reported. The use of water as a green medium provides sustainability by avoiding the use of toxic solvents. These findings propose a sustainable and straightforward strategy for generating interesting N-containing heterocyclic compounds.
MOLECULAR DIVERSITY
(2023)
Article
Engineering, Electrical & Electronic
Junhui Chen, Yanqing Gao, Jie Yang, Qiangfei Wang, Yupeng Cao, Kai Jiang, Yawei Li, Jinzhong Zhang, Liangqing Zhu, Liyan Shang, Zhigao Hu, Junhao Chu
Summary: Two-dimensional transition metal dichalcogenides (TMDCs) have been extensively studied due to their atomic thin structure and unique optoelectronic properties. In this work, vertical MoSe2/WSe2 p-n heterojunctions were successfully synthesized, showing excellent rectification characteristics, photoresponse, and optical response time, suggesting their potential as a candidate material for high-performance optoelectronic devices.
IEEE ELECTRON DEVICE LETTERS
(2022)
Article
Chemistry, Organic
Han Hao, Thibault Bagnol, Mathieu Pucheault, Laurel L. Schafer
Summary: By utilizing a specific titanium precatalyst, the hydroamination of alkynylphosphines to prepare 2-amino-phosphines in high yield and on gram scale has been achieved. The broad substrate scope allows for the rapid assembly of a variety of ligands in one pot, making preparation of metal P,N-complexes for catalysis or small molecule activation easy and efficient.
Article
Materials Science, Multidisciplinary
Yi Wei, Zining Li, Jiajing Feng, Yu Chen, Jianqi Zhang, Yawen Li, Wei Jiang, Tianrui Zhai, Yuze Lin, Zhixiang Wei, Zhaohui Wang, Ningning Liang
Summary: Multifunctional integration of optoelectronic devices in a single photodiode is vital for future on-chip multifunctional integration and Internet of Things applications. In this study, a p-i-n heterojunction organic photodiode with optimized vertical phase distribution and solid-state packing was fabricated using a layer-by-layer deposition method. The photodiode exhibited suppressed trap state densities and reduced energy disorder, resulting in improved performance in both self-powered photodetector and organic photovoltaic modes. This research provides important insights into trap density and energy disorder suppression in solution-processed multifunctional integrated photoelectric conversion diodes.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Robbert Q. Kim, Mohit Misra, Alexis Gonzalez, Ines Tomaskovic, Donghyuk Shin, Hermann Schindelin, Dmitri V. Filippov, Huib Ovaa, Ivan Dikic, Gerbrand J. Van der Heden van Noort
Summary: Legionnaires' disease is caused by Legionella pneumophila, which uses an unconventional ubiquitination process to replicate within host cells. This study synthesized analogues to investigate the mechanism and potential for new treatments. Synthetic Ub(ADPr) analogues were effective in pulling down overexpressed DupA from cell lysate.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Joanna Liwocha, David T. Krist, Gerbrand J. van der Heden van Noort, Fynn M. Hansen, Vinh H. Truong, Ozge Karayel, Nicholas Purser, Daniel Houston, Nicole Burton, Mark J. Bostock, Michael Sattler, Matthias Mann, Joseph S. Harrison, Gary Kleiger, Huib Ovaa, Brenda A. Schulman
Summary: The study reveals that the aliphatic side chain specifying reactive amine geometry is a determinant of the ubiquitin code, affecting the assembly of ubiquitin chains by replacing the acceptor site.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guido Janssen, Susan Zhang, Remco Merkx, Christa Schiesswohl, Champak Chatterjee, K. Heran Darwin, Paul P. Geurink, Gerbrand J. van der Heden van Noort, Huib Ovaa
Summary: Tuberculosis is a global health problem caused by Mycobacterium tuberculosis infection. New drugs targeting Mtb are urgently needed due to the existence of drug resistant strains. The Pup proteasome system has been identified as a potential target for drug development, with inhibitors of Dop and PafA showing promising results in inhibiting Mtb activity.
Article
Biochemistry & Molecular Biology
Rukmini Mukherjee, Anshu Bhattacharya, Denisa Bojkova, Ahmad Reza Mehdipour, Donghyuk Shin, Khadija Shahed Khan, Hayley Hei-Yin Cheung, Kam-Bo Wong, Wai-Lung Ng, Jindrich Cinatl, Paul P. Geurink, Gerbrand J. van der Heden van Noort, Krishnaraj Rajalingam, Sandra Ciesek, Gerhard Hummer, Ivan Dikic
Summary: Famotidine has no effect on viral replication but can modulate specific signaling pathways, reducing TLR3-dependent signaling processes and subsequent activation of IRF3 and the NF-kappa B pathway, leading to control of antiviral and inflammatory responses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Cell Biology
Yara Huppelschoten, Gerbrand J. van der Heden van Noort
Summary: Protein ubiquitination is a crucial post-translational modification that regulates cellular processes and prevents diseases. Understanding the molecular mechanisms of ubiquitination is important for deciphering this process. Chemical preparation of ubiquitin conjugates offers advantages in terms of purity and chemo-selectivity, aiding in the decoding of ubiquitin signaling.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Tom J. Harryvan, Marten Visser, Linda de Bruin, Leonie Plug, Lisa Griffioen, Arend Mulder, Peter A. van Veelen, Gerbrand J. van Der Heden van Noort, Marlieke Lm Jongsma, Miranda H. Meeuwsen, Emmanuel Jhj Wiertz, Saskia J. Santegoets, James Ch Hardwick, Thorbald Van Hall, Jacques Neefjes, Sjoerd H. Van der Burg, Lukas J. A. C. Hawinkels, Els M. E. Verdegaal
Summary: This study found that human CRC-derived CAFs have an enhanced ability to cross-present antigens, which involves the participation of lysosomal protease cathepsin S. The interaction between cross-presenting CAFs and CD8+ T cells suppresses T cell function.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Dharjath S. Hameed, Huib Ovaa, Gerbrand J. van der Heden J. van Noort, Aysegul Sapmaz
Summary: This study presents the design and development of peptides that preferentially inhibit UCH-L5 and demonstrate their ability to permeate cells and accumulate polyubiquitinated substrates, making UCH-L5 an attractive target for therapeutic purposes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yara Huppelschoten, Angela F. Elhebieshy, Dharjath S. Hameed, Aysegul Sapmaz, Jens Buchardt, Thomas E. Nielsen, Huib Ovaa, Gerbrand J. van der Heden van Noort
Summary: Chemical protein synthesis is a powerful tool to obtain uniformly modified proteins. This study successfully synthesized a 123 amino-acid nanobody against GFP using a chemical ligation-desulfurization strategy. The synthetic nanobody was functionalized with a propargyl moiety and further modified with biotin or a sulfo-cyanine5 dye. These modifications allowed for in vitro validation and microscopy imaging.
Article
Chemistry, Multidisciplinary
Jim Voorneveld, Max S. Kloet, Sven Wijngaarden, Robbert Q. . Kim, Angeliki Moutsiopoulou, Marnix Verdegaal, Mohit Misra, Ivan Dikic, Gijsbert A. . van der Marel, Herman S. Overkleeft, Dmitri V. Filippov, Gerbrand J. van der Heden van Noort
Summary: This article describes the development and optimization of a methodology for preparing peptides and proteins modified on the arginine residue with an ADPr group. The authors successfully obtained four regioisomers of ADP-ribosylated ubiquitin using this method and investigated the activity and selectivity of Legionella pneumophila ligase and hydrolase enzymes through biochemical experiments.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Yara Huppelschoten, Rishov Mukhopadhyay, Jens Buchardt, Thomas E. E. Nielsen, Alfred C. O. Vertegaal, Huib Ovaa, Gerbrand J. van Der Heden J. van Noort
Summary: Researchers synthesized a large number of SUMO-based fluorescence polarization reagents to study enzyme preferences. Screening of five SENPs revealed differences in deconjugation activity and substrate preferences. The findings provide important evidence for understanding the mechanism of SENP-specific binding to SUMO.
Article
Biochemistry & Molecular Biology
Ana G. Pedrosa, Tania Francisco, Tony A. Rodrigues, Maria J. Ferreira, Gerbrand J. van der Heden van Noort, Jorge E. Azevedo
Summary: The study investigated the extraction mechanism of PEX5 using a cell-free in vitro system and various engineered molecules. It was found that ubiquitinated PEX5 can be extracted, and the N- and C-terminus of PEX5 are not crucial for the extraction process. The study also revealed that the PEX5-linked monoubiquitin is unfolded prior to extraction, and intramolecularly cross-linked ubiquitin at residue 11 of PEX5 inhibits extraction.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Bianca D. M. van Tol, Bjorn R. van Doodewaerd, Guinevere S. M. Lageveen-Kammeijer, Bas C. Jansen, Cami M. P. Talavera Ormeno, Paul J. M. Hekking, Aysegul Sapmaz, Robbert Q. Kim, Angeliki Moutsiopoulou, David Komander, Manfred Wuhrer, Gerbrand J. van der Heden van Noort, Huib Ovaa, Paul P. Geurink
Summary: In this study, the authors develop a mass spectrometric assay to probe the activity of deubiquitinases on all diubiquitins simultaneously under substrate competition conditions, providing insights into their linkage selectivity.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yara Huppelschoten, Jens Buchardt, Thomas E. Nielsen, Aysegul Sapmaz, Gerbrand J. van der Heden van Noort
Summary: Autophagy is a cellular process that degrades materials within cells through the formation of autophagosomes. Autophagy-related proteins, including LC3A and LC3B, play a key role in autophagosome formation. The conjugation of LC3 to a phospholipid allows for the elongation of the autophagosome and facilitates the delivery of proteins for degradation. Dysregulation of autophagy has been linked to various diseases, making components of this pathway potential therapeutic targets. This study describes the synthesis of LC3 activity-based probes using native chemical ligation, which can provide insights into the mechanistic and structural details of autophagy.
Article
Biochemistry & Molecular Biology
Gabrielle B. A. van Tilburg, Andrea G. Murachelli, Alexander Fish, Gerbrand J. van der Heden van Noort, Huib Ovaa, Titia K. Sixma
Summary: This study investigates the response of the deubiquitinase UCHL3 to lysine 27-linked ubiquitin chains using synthetic ubiquitin tools, revealing that (K27)Ub(2) serves as a natural inhibitor of UCHL3. By establishing a quantitative kinetic model of the inhibitory mechanism, it is shown that (K27)Ub(2) can inhibit UCHL3 covalently and allosterically.
CELL CHEMICAL BIOLOGY
(2021)