期刊
THROMBOSIS AND HAEMOSTASIS
卷 114, 期 5, 页码 969-981出版社
SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN
DOI: 10.1160/TH14-09-0727
关键词
MEG-01 cells; raptor; rictor; mTOR; rapamycin; torin1; apoptosis; calcium homeostasis
资金
- MEC [BFU2013-45564C2-1-p]
- Junta de Extremadura-FEDER [GR10010, PRIBS10020]
- NIH Carlos III Health Program [FI10/00573]
- University of Extremadura Posdoc-Research Contract [D-01]
The function of the mammalian target of rapamycin (mTOR) is up-regulated in response to cell stimulation with growing and differentiating factors. Active mTOR controls cell proliferation, differentiation and death. Since mTOR associates with different proteins to form two functional macromolecular complexes, we aimed to investigate the role of the mTOR1 and mTOR2 complexes in MEG-01 cell physiology in response to thrombopoietin (TPO). By using mTOR antagonists and overexpressing FKBP38, we have explored the role of both mTOR complexes in proliferation, apoptosis, maturation-like mechanisms, endo-plasmic reticulum-stress and the intracellular location of both active mTOR complexes during MEG-01 cell stimulation with TPO. The results demonstrate that mTOR1 and mTOR2 complexes play different roles in the physiology of MEG-01 cells and in the maturation-like mechanisms; hence, these findings might help to understand the mechanism underlying generation of platelets.
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