Article
Optics
Ignacio Iglesias
Summary: This non-interferometric technique offers a simple way to achieve quantitative phase microscopy by rotating a mask to detect wavefront gradient. The method also includes calibrating the sensor and processing signals for phase extraction. Three-dimensional images of polystyrene microspheres immersed in index-matching liquid were successfully obtained using this technique.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Sunil Bhatt, Ankit Butola, Sheetal Raosaheb Kanade, Anand Kumar, Dalip Singh Mehta
Summary: The study introduces a method using filtered white light and deep neural network for single-shot phase-shifting interferometry, enabling accurate phase measurement. By training the network on two different samples, high-resolution phase recovery for various biomedical applications can be achieved in the future.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Optics
Sunil Bhatt, Ankit Butola, Anand Kumar, Pramila Thapa, Akshay Joshi, Suyog Jadhav, Neetu Singh, Dilip K. Prasad, Krishna Agarwal, Dalip Singh Mehta
Summary: Multispectral quantitative phase imaging (MS-QPI) is achieved by using a highly spatially sensitive digital holographic microscope assisted by a deep neural network to extract spectral dependent quantitative information in single-shot. Three different wavelengths (532, 633, and 808 nm) are used, and interferometric data is acquired for each wavelength. A generative adversarial network is trained to generate multispectral (MS) quantitative phase maps from a single input interferogram. The validation of the approach is done by comparing the predicted MS phase maps with numerically reconstructed phase maps using different image quality assessment metrics.
Article
Optics
Patrick Ledwig, Francisco E. Robles
Summary: This study demonstrates a 3D refractive index tomography with a large field of view in opaque, arbitrarily thick scattering samples, overcoming the limitations of traditional methods. The approach is theoretically analyzed, simulated, and experimentally validated, showing significant potential for biomedical applications.
Article
Multidisciplinary Sciences
Manoj Kumar, Lavlesh Pensia, Raj Kumar
Summary: A new configuration of a digital holographic system is proposed to double the camera field-of-view (FOV) of the existing setup. This is achieved by multiplexing the object beam with two different spatial frequencies using a Fresnel bi-prism. The feasibility of the proposed system is demonstrated experimentally, and its limitations and a method to overcome them are discussed. The system has practical applications in microscopy and optical metrology.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Wenwen Chen, Yingying Xie, Yuang Chang, Yuhai Xu, Mengqian Zhao, Pengwei Deng, Jianhua Qin, Hongjing Li
Summary: A new strategy utilizing chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow was proposed for isolating circulating exosomes in a portable device, achieving a recovery rate of over 80% within 20 minutes. The method outperforms traditional ultracentrifugation and allows for in situ lysis and further analysis of RNA and proteins from the exosomes isolated from samples.
Article
Biochemical Research Methods
Xiang Dai, Shiqi Xu, Xi Yang, Kevin C. Zhou, Carolyn Glass, Pavan Chandra Konda, Roarke Horstmeyer
Summary: This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier ptychography, which is a synthetic aperture-based imaging approach to improve resolution with phaseless measurements, to additionally account for the vectorial nature of light.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Optics
Chen-Yang Li, Jin-Bao Long, Ming-Qi Huang, Bin Chen, Yu-Meng Yang, Xiao Jiang, Chuan-Fang Xiang, Zhao-Li Ma, De-Qiang He, Luo-Kan Chen, Shuai Chen
Summary: The study introduces a portable and robust home-built atom gravimeter (USTC-AG11) that operated continuously in a seismic station in Zhaotong, Yunnan for over five months. Using matter-wave interference principle, the atom gravimeter achieved high sensitivity to local gravity with micro-Gal level precision. The implementation of vibrational compensation technique significantly reduced noise levels and enabled long-term continuous operation of the atom gravimeter.
Article
Pharmacology & Pharmacy
Jennifer Cauzzo, Nikhil Jayakumar, Balpreet Singh Ahluwalia, Azeem Ahmad, Natasa Skalko-Basnet
Summary: The study identifies limitations of traditional methods, introduces QPM as a promising complementary technique, and successfully locates and tracks liposomes.
Article
Materials Science, Multidisciplinary
Carlos Cairos, Ricardo Oliva-Garcia, Gabriela Siverio, Juan Manuel Trujillo-Sevilla, Jose Manuel Rodriguez-Ramos, Angel Acebes
Summary: The refractive index (RI) of biological tissues is an important optical property that can provide valuable information about tissue composition. This study reviews available methods to measure RI in biological tissues, with a focus on quantitative phase imaging (QPI) and its ability to obtain RI maps and three-dimensional projections of transparent samples. A simple QPI method is proposed to estimate the RI of complex materials without prior knowledge of sample thickness, using the linear relationship between optical path difference (OPD) and RI of the medium. The findings of this study categorize a set of immersion media, identify error sources, and demonstrate the applicability of the QPI method in heterogeneous biological tissues.
Review
Chemistry, Analytical
Chunfeng Duan, Jiamin Li, Yuanhao Zhang, Kun Ding, Xuhui Geng, Yafeng Guan
Summary: On-site analysis provides qualitative and/or quantitative information of samples at their location, and portable instruments have been widely used for this purpose. Sample preparation plays an important role in most applications due to the limitations of instrument detection and selectivity. This review summarizes the recent advances in on-site sample preparation devices, on-site GC/GC-MS, and on-site optical instruments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
X. Chen, C. Caradeuc, A. Montagne, V. Baud, G. Bertho, C. Lucas-Torres, N. Giraud
Summary: Accurate quantification of metabolites by NMR is crucial for understanding metabolic pathways and addressing diseases. A new analytical protocol using pure shift library of calibration reference spectra has been developed to determine metabolite concentrations. This method, validated through model mixtures, provides quantitative and high-resolution vision of metabolic processes in a time compatible with metabolomics studies. Furthermore, it has been successfully applied to determine absolute metabolite concentrations in a lymphoma extracellular medium.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Hsiao-Ning Weng, Chu-Yu Huang
Summary: The proposed common-path off-axis interferometric system eliminates zero-order artifacts and increases the phase image quality by compounding multi-angle holograms. The high-quality quantitative phase imaging maintains imaging frame rate while revealing more detailed cell structures.
RESULTS IN PHYSICS
(2021)
Review
Chemistry, Analytical
Stylianos Vasileios Kontomaris, Andreas Stylianou, Georgios Chliveros, Anna Malamou
Summary: Measuring the mechanical properties of biological samples at the nanoscale using AFM has opened up new possibilities in studying and detecting various diseases at early stages. It is believed that AFM techniques will play a central role in disease diagnosis and modeling in the future, using rigorous mathematical criteria for automated and user-independent diagnosis.
Article
Optics
Gaston A. Ayubi, M. Fernandez Lakatos, Nicolas Casaballe, Erna Frins
Summary: A new quantitative approach for phase retrieval is presented, which is derived from analyzing the light intensity propagation through a phase object and a sinusoidal intensity mask. Unlike the Transport of Intensity Equation (TIE), this new algorithm is simpler as it does not involve the Laplacian of the phase, and it can be easily implemented in practical applications.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Biochemical Research Methods
Noga Nissim, Matan Dudaie, Itay Barnea, Natan T. Shaked
Summary: A method for real-time visualization and automatic processing for detection and classification of untreated cancer cells in blood was presented using digital holographic microscopy and machine learning. The study achieved high accuracy in automatically classifying primary and metastatic cancer cells, as well as blood cells, providing a foundation for further automatic enrichment and cancer cell grading.
Article
Optics
Simcha K. Mirsky, Natan T. Shaked
Summary: Six-pack holography is used to reject out-of-focus objects in dynamic samples by illuminating the sample from six different angles and pixel-wise averaging the reconstructed images. This significantly reduces the contribution of out-of-focus objects by up to 83%, improving system sectioning capabilities.
Article
Biochemical Research Methods
Rongli Guo, Itay Barnea, Natan T. Shaked
Summary: The proposed multimodal imaging technique combines tomographic phase microscopy with limited angular projection range and two-channel spinning-disk confocal scanning fluorescence microscopy. It allows high-accuracy 3D refractive index profiling of live cells, providing both cellular morphology and molecular specificity. The additional 3D morphological details act as constraints to improve the accuracy of RI reconstruction, demonstrating the superiority of the technique in various samples.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Physics, Multidisciplinary
Rongli Guo, Itay Barnea, Natan T. Shaked
Summary: This paper introduces a wide-field interferometric imaging module that utilizes shearing interferometry with constant off-axis angle and a low-coherence light source to provide quantitative phase profiles with lower spatial coherent noise. The module allows full control of the shearing distance between interfering beams and can be used as an add-on unit to a conventional bright-field microscope for biomedical and metrological measurements.
FRONTIERS IN PHYSICS
(2021)
Article
Physics, Multidisciplinary
Lidor Karako, Rongli Guo, Itay Barnea, Natan T. Shaked
Summary: This study presents a new imaging platform for stain-free quantitative imaging of biological cells, successfully addressing phase ambiguities. The method not only allows for rapid data acquisition, but also is suitable for observing dynamic processes.
FRONTIERS IN PHYSICS
(2021)
Article
Optics
Simcha K. Mirsky, Natan T. Shaked
Summary: A new six-pack holography (6PH) system is presented in this paper, which can acquire six off-axis holograms simultaneously from two fields of view and three wavelengths in a single exposure. Compared with previous systems, the system is based on an external module, providing a more elegant solution. It is also the first time that six-pack holography is utilized for multi-wavelength imaging, and volumetric data of flowing erythrocytes with twice the field of view of previous techniques are acquired using the new system.
APPLIED PHYSICS B-LASERS AND OPTICS
(2022)
Article
Biochemical Research Methods
Almog Taieb, Garry Berkovic, Miki Haifler, Ori Cheshnovsky, Natan T. Shaked
Summary: We propose a multimodal label-free optical measurement approach for analyzing sliced tissue biopsies using quantitative phase imaging and localized Raman spectroscopy. The approach involves performing label-free quantitative phase imaging of the tissue slice and then using kernelled structural support vector machine to segment the tissue layers. The segmented areas are used to guide localized Raman spectroscopy measurements for classification of tissue types. The results show that the prior segmentation using quantitative phase imaging improves the classification accuracy of Raman spectra.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Nanoscience & Nanotechnology
Simcha K. Mirsky, Itay Barnea, Natan T. Shaked
Summary: The article presents a dynamic scan-free optical tomography approach for stain-free 3D imaging of biological cells. The technique uses a new double six-pack tomography technique to capture 12 off-axis holograms in a single camera exposure without sacrificing resolution or field of view. Experimental results show that the proposed technique achieves a RI accuracy of 98.5% for tomograms.
Article
Cell Biology
Mayssam Nassir, Mattan Levi, Gili Dardikman-Yoffe, Simcha K. Mirsky, Natan T. Shaked
Summary: We present a multidisciplinary approach for predicting the 3D swimming of sperm cells with various morphologies, and establish a numerical mechanical model for analysis. This method is significant for sperm analysis, male-infertility diagnosis, and sperm selection in fertility treatments.
Biographical-Item
Optics
Natan T. Shaked, YongKeun Park, Stephen A. Boppart, Adam Wax, Peter T. C. So
Summary: Gabriel Popescu will be remembered as a creative leader in biophotonics, making pioneering contributions to quantitative phase imaging and spectroscopy. He was also known as an engaging collaborator and a dear friend.
Article
Biochemical Research Methods
Lioz Noy, Itay Barnea, Simcha K. Mirsky, Dotan Kamber, Mattan Levi, Natan T. Shaked
Summary: In this study, a new method using unstained imaging data and deep learning techniques was proposed to predict DNA fragmentation in sperm cells. By comparing with cells imaged using fluorescence microscopy, we obtained the accuracy of the prediction model and found a low mean absolute error. This method has the potential to improve cell selection during ICSI.
Article
Cell Biology
Mayssam Nassir, Mattan Levi, Natan T. Shaked
Summary: This study created a 3D mechanical model to simulate the swimming behavior of human sperm cells in the female genital tract. The study found that the chances of abnormal sperm cells reaching the oocyte site were influenced by the complex geometry and dynamics of the uterus.
Article
Automation & Control Systems
Lioz Noy, Itay Barnea, Matan Dudaie, Dotan Kamber, Mattan Levi, Natan T. Shaked
Summary: This paper proposes a new model based on a multilayer long short-term memory (LSTM) network and linear extrapolation calculation to predict the future location of individual sperm cells based on their previous paths. The model is trained with a unique loss function constructed from the predicted location and trajectory, resulting in low mean location error predictions. This model can provide faster and more accurate sperm motility predictions, better tracking, and aid automatic sperm capturing technologies.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Automation & Control Systems
Anat Cohen, Matan Dudaie, Itay Barnea, Francesca Borrelli, Jaromir Behal, Lisa Miccio, Pasquale Memmolo, Vittorio Bianco, Pietro Ferraro, Natan T. Shaked
Summary: A new label-free imaging flow cytometry method is proposed for noninvasive and automated biological cell classification. By acquiring off-axis holograms of each cell from multiple viewpoints and using a modified ResNet-18 deep convolutional neural network, highly discriminating features are extracted for cell detection, classification, and differentiation. The method shows improved accuracy in classifying different types of cells and reduces the number of cell examples needed for training.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Automation & Control Systems
Keren Ben-Yehuda, Simcha K. Mirsky, Mattan Levi, Itay Barnea, Inbal Meshulach, Sapir Kontente, Daniel Benvaish, Rachel Cur-Cycowicz, Yoav N. Nygate, Natan T. Shaked
Summary: A new technique using quantitative stain-free interferometric imaging for sperm analysis can evaluate DNA fragmentation, morphology, and motility on the same live cell. Traditional clinical practice requires different staining protocols for morphological and DNA analysis, which leads to inconsistencies in fertility evaluation. The proposed method, incorporating deep learning, can accurately evaluate sperm cells, potentially reducing uncertainty in infertility diagnosis and increasing treatment success rates.
ADVANCED INTELLIGENT SYSTEMS
(2022)
