Article
Optics
Yueqiang Zhu, Chen Zhang, Yanyan Gong, Wei Zhao, Jintao Bai, Kaige Wang
Summary: The investigation introduces a method utilizing SSP for generating independent multifocal spots through a high-NA lens, validated through numerical simulation and experimental results indicating high uniformity and consistency.
Article
Optics
Conghao Wang, Huilan Liu, Jianrui Ma, Haodong Cui, Yijun Li, Danlei Wu, Yanhui Hu, Dakun Wu, Qiang Fu, Lintang Liang, Fei Yu, Runlong Wu, Aimin Wang, Lishuang Feng
Summary: Two-photon endomicroscopy is an optical biopsy tool that meets clinical requirements for real-time subcellular-resolution imaging, assisting in pathological biopsy diagnosis. The system utilizes a piezoelectric ceramic tube scanner, a double-cladding antiresonant fiber for light-pulse transmission, and achieves a field of view of around 200 µm with a resolution of 3.1 µm and a frame rate of 0.7 fps.
Article
Optics
Jiapu Yan, Zhichao Ye, Tingting Jiang, Shiqi Chen, Huajun Feng, Zhihai Xu, Qi Li, Yueting Chen
Summary: In this study, we proposed a field of view attention image restoration model for continuous zooming to address the optical aberrations of Alvarez lenses. By simulating degraded images and conducting restoration experiments, we demonstrated that our method outperforms compared methods in restoring degraded images across various zoom configurations, while also exhibiting strong generalization capabilities under untrained configurations.
Article
Optics
Jiapu Yan, Zhichao Ye, Tingting Jiang, Shiqi Chen, Huajun Feng, Zhihai Xu, Qi Li, Yueting Chen
Summary: We propose a field of view attention image restoration model for continuous zooming, which simulates degraded images by optimizing the polynomial of Alvarez lenses and achieves better restoration results compared to other methods.
Article
Biochemical Research Methods
Jaehun Jeon, Hyunwoo Kim, Hyunwoo Jang, Kyungmin Hwang, Kyuyoung Kim, Young-Gyun Park, Ki-Hun Jeong
Summary: A handheld confocal microscope catheter using a rapid MEMS scanning mirror is capable of real-time optical biopsy, providing high-definition and high frame rate scanning.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Optics
Dongmok Kim, Younghoon Shin, Hyuk-Sang Kwon
Summary: The MMM system suffers from ghost images caused by scattered emission photons, degrading image quality. A novel strategy based on Monte-Carlo simulation was proposed to rapidly reduce noise in the MMM system, successfully eliminating ghost images and reducing computational cost.
CURRENT OPTICS AND PHOTONICS
(2021)
Article
Optics
Siddharth Sivankutty, Andrea Bertoncini, Victor Tsvirkun, Naveen Gajendra Kumar, Gaelle Brevalle, Geraud Bouwmans, Esben Ravn Andresen, Carlo Liberale, Herve Rigneault
Summary: This study presents a high-efficiency, miniaturized coherent beam that can be combined directly on the tip of a multicore fiber bundle, allowing for two-photon imaging. The compact device footprint facilitates its incorporation into a minimally invasive ultra-thin nonlinear endoscope.
Article
Public, Environmental & Occupational Health
Mohamed Yassin, Adrian Clifford, Heather Dixon, Curtis J. Donskey
Summary: The study aims to evaluate the dryness of endoscope channels after an automated reprocessing process. The results show that there is still residual moisture in the channels after routine disinfection, especially in the narrower air/water channels. An extended 10-minute air flush can effectively dry the larger biopsy/suction channel but has limited effect on the air/water channels.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Public, Environmental & Occupational Health
Megan M. Wallace, Taylor Keck, Heather Dixon, Mohamed Yassin
Summary: The value of routine visual inspections and surveillance of endoscopes was evaluated by performing borescope examinations and microbial sampling. The results showed positive microbial cultures in 28% of respiratory endoscopes, 22% of GI endoscopes, and 30% of urological endoscopes. Borescope examinations revealed multiple abnormalities and damage, leading to timely repair and discontinuation of damaged endoscopes.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Chemistry, Physical
Alexander Mikhaylov, Ryan N. Wilson, Kristen M. Parzuchowski, Michael D. Mazurek, Charles H. Camp, Martin J. Stevens, Ralph Jimenez
Summary: The research found that a signal similar to entangled two-photon absorption (E2PA) can be observed under the hot-band absorption (HBA) mechanism. Under typical conditions for E2PA measurements, contributions from the HBA process could lead to an overestimation of the quantum advantage by several orders of magnitude.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Optics
Huiying Zhang, Xu Wang, Hanwen Du, Haiyang Yu, Jinghao Wu, Yanlong Meng, Yanqing Qiu, Bangning Mao, Pengwei Zhou, Yi Li
Summary: This Letter proposes a simple and complete solution to improve the imaging quality of single fiber scanners by combining piezoelectric sensors and machine learning algorithms. The experimental results demonstrate excellent stability and accuracy of the proposed self-calibration SFS.
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Multidisciplinary Sciences
Peter R. Corridon
Summary: This study examines the microarchitecture of acellular kidney scaffolds using intravital microscopy (IVM) data. The effectiveness of decellularization and the performance of acellular nephron compartments after transplantation were evaluated. The results contribute to the broader understanding and application of this imaging technique in analyzing scaffold systems.
Article
Optics
Mareddi Bharath Kumar, Daekyung Kang, Mohammad Awwal Adeshina, Soobae Kim, Taewan Kim, Jonghoo Park
Summary: The electro-optic diffractive multifocal lens combines multiple focal points and can correct complex visual problems through electrical switching without compromising the field of view, providing new possibilities for ophthalmic applications.
OPTICS AND LASERS IN ENGINEERING
(2021)
Article
Optics
David H. Lippman, Greg R. Schmidt, Julie L. Bentley, Duncan T. Moore, Hooman Akhavan, John Paul Harmon, George M. Williams
Summary: In this work, gradient-index Alvarez lenses (GALs), a new type of freeform optical component, are surveyed for their unique properties in generating variable optical power. GALs display behavior similar to conventional surface Alvarez lenses (SALs) through a recently achievable refractive index distribution in fabrication. The authors present a first-order framework for GALs, including analytical expressions for their refractive index distribution and power variation. The study demonstrates the importance of three-dimensional higher-order refractive index terms in the optimized design of GALs. Additionally, a fabricated GAL is showcased, with power measurements closely aligning with the developed first-order theory.
Biographical-Item
Chemistry, Physical
Warren Zipfel, Chris Xu, Elliot Elson, Sudipta Maiti
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Neurosciences
Bin Sun, Mengran Wang, Anna Hoerder-Suabedissen, Chris Xu, Adam M. Packer, Francis G. Szele
Summary: We demonstrate, for the first time, three-photon microscopy imaging of the SVZ in live mice. This strategy could be useful for intravital visualization of cell dynamics, molecular, and pathological perturbation and regenerative events.
Article
Immunology
Kibaek Choe, Yusaku Hontani, Tianyu Wang, Eric Hebert, Dimitre G. Ouzounov, Kristine Lai, Ankur Singh, Wendy Beguelin, Ari M. Melnick, Chris Xu
Summary: Intravital three-photon microscopy allows for the visualization of immune cell behavior in mouse lymph nodes at depths of 600-900 μm. This technique enables measurement of migration ability in different depths and the observation of inflammation effects on T cell motility.
Article
Biochemical Research Methods
Najva Akbari, Mihailo R. Rebec, Fei Xia, Chris Xu
Summary: Multiphoton fluorescence microscopy uses long excitation wavelengths and nonlinear excitation to increase penetration depth and suppress out-of-focus fluorescence for deep in vivo imaging. However, the imaging depth is limited by tissue scattering and absorption. Three-photon fluorescence microscopy has been developed to overcome this limitation and achieve unprecedented deep in vivo imaging. Further technological development is needed to fully exploit the potential of three-photon microscopy.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Aron T. Mok, Jamien Shea, Chunyan Wu, Fei Xia, Rose Tatarsky, Nilay Yapici, Chris Xu
Summary: We developed a simple and flexible setup to measure tissue ballistic and total transmission. The measurements were performed on biological samples ex vivo, and spatially resolved transmission maps were obtained to reveal transmission heterogeneity. The method can be applied to measure transmission of other samples and has valuable implications for multiphoton microscopy.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biology
Max Jameson Aragon, Aaron T. Mok, Jamien Shea, Mengran Wang, Haein Kim, Nathan Barkdull, Chris Xu, Nilay Yapici
Summary: A multiphoton imaging method was developed to capture neural structure and activity in behaving flies through the intact cuticle, revealing high transmission at wavelengths >900nm. By compressing or removing air sacs, multiphoton imaging of the fly brain through the intact cuticle was achieved with superior results using 3-photon imaging in deeper regions. This through-cuticle imaging method extends the time limits of in vivo imaging in flies and offers new ways to study neural structure and activity in the fly brain.
Article
Multidisciplinary Sciences
Yusaku Hontani, Najva Akbari, Kristine E. Kolkman, Chunyan Wu, Fei Xia, Kibaek Choe, Grace Zhang Wang, Mariya Sokolova, Joseph R. Fetcho, Chris Xu
Summary: Multiphoton microscopy techniques, such as two-photon microscopy (2PM) and three-photon microscopy (3PM), are powerful tools for deep-tissue in vivo imaging with subcellular resolution. Three-photon microscopy has advantages over two-photon microscopy in terms of longer attenuation length and less background fluorescence generation. Commercial laser sources and microscopes are available for 3PM, making it readily accessible for biology research laboratories.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Neurosciences
David Sinefeld, Fei Xia, Mengran Wang, Tianyu Wang, Chunyan Wu, Xusan Yang, Hari P. Paudel, Dimitre G. Ouzounov, Thomas G. Bifano, Chris Xu
Summary: This study presents an AO system for 3PM in vivo mouse brain imaging, which achieves deeper imaging using a femtosecond source and MEMS SLM for phase correction.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Neurosciences
Ahmed S. Abdelfattah, Sapna Ahuja, Taner Akkin, Srinivasa Rao Allu, Joshua Brake, David A. Boas, Erin M. Buckley, Robert E. Campbell, Anderson Chen, Xiaojun Cheng, Tomas Cizmar, Irene Costantini, Massimo De Vittorio, Anna Devor, Patrick R. Doran, Mirna El Khatib, Valentina Emiliani, Natalie Fomin-Thunemann, Yeshaiahu Fainman, Tomas Fernandez-Alfonso, Christopher G. L. Ferri, Ariel Gilad, Xue Han, Andrew Harris, Elizabeth M. C. Hillman, Ute Hochgeschwender, Matthew G. Holt, Na Ji, Kivilcim Kilic, Evelyn M. R. Lake, Lei Li, Tianqi Li, Philipp Machler, Evan W. Miller, Rickson C. Mesquita, K. M. Naga Srinivas Nadella, U. Valentin Nagerl, Yusuke Nasu, Axel Nimmerjahn, Petra Ondrackova, Francesco S. Pavone, Citlali Perez Campos, Darcy S. Peterka, Filippo Pisano, Ferruccio Pisanello, Francesca Puppo, Bernardo L. Sabatini, Sanaz Sadegh, Sava Sakadzic, Shy Shoham, Sanaya N. Shroff, R. Angus Silver, Ruth R. Sims, Spencer L. Smith, Vivek J. Srinivasan, Martin Thunemann, Lei Tian, Lin Tian, Thomas Troxler, Antoine Valera, Alipasha Vaziri, Sergei A. Vinogradov, Flavia Vitale, Lihong Wang, Hana Uhlirova, Chris Xu, Changhuei Yang, Mu-Han Yang, Gary Yellen, Ofer Yizhar, Yongxin Zhao
Summary: This article reviews a diverse toolkit of novel methods for exploring brain function that have emerged from the BRAIN Initiative and related large-scale efforts, with a focus on neurophotonic tools applicable to animal studies. It provides an outlook for future directions in the field.
Article
Optics
Sylvain Gigan, Ori Katz, Hilton B. de Aguiar, Esben Ravn Andresen, Alexandre Aubry, Jacopo Bertolotti, Emmanuel Bossy, Dorian Bouchet, Joshua Brake, Sophie Brasselet, Yaron Bromberg, Hui Cao, Thomas Chaigne, Zhongtao Cheng, Wonshik Choi, Tomas Cizmar, Meng Cui, Vincent R. Curtis, Hugo Defienne, Matthias Hofer, Ryoichi Horisaki, Roarke Horstmeyer, Na Ji, Aaron K. LaViolette, Jerome Mertz, Christophe Moser, Allard P. Mosk, Nicolas C. Pegard, Rafael Piestun, Sebastien Popoff, David B. Phillips, Demetri Psaltis, Babak Rahmani, Herve Rigneault, Stefan Rotter, Lei Tian, Ivo M. Vellekoop, Laura Waller, Lihong Wang, Timothy Weber, Sheng Xiao, Chris Xu, Alexey Yamilov, Changhuei Yang, Hasan Yilmaz
Summary: In the last decade, various tools such as wavefront shaping and computational methods have been developed to understand and control the propagation of light in complex mediums. This field has revolutionized the possibility of diffraction-limited imaging at depth in tissues, and a vibrant community is actively working on it.
JOURNAL OF PHYSICS-PHOTONICS
(2022)
Editorial Material
Optics
Chris Xu
Summary: The research group has developed a skull-clearing technique that enables stable, high resolution optical imaging of the mouse brain through a transparent window for several weeks.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Najva Akbari, Rose L. Tatarsky, Kristine E. Kolkman, Joseph R. Fetcho, Andrew H. Bass, Chris Xu
Summary: Danionella, a neuroscience model, allows scientists to study the entire depth and rostral-caudal extent of the adult vertebrate brain using two- and three-photon microscopy.
Article
Biochemical Research Methods
Aaron K. Laviolette, Dimitre G. Ouzounov, Chris Xu
Summary: Measurements of three-photon action cross-sections for fluorescein in water with pH -11.5 were conducted using a femtosecond wavelength tunable non-collinear optical parametric amplifier. The excitation wavelength range was from 1154 to 1500 nm in 50 nm steps. The results confirmed the cube-law power dependance and revealed differences in the three-photon excitation spectrum compared to the one- and two-photon excitation spectra. The observed significant difference in three-photon action cross-sections at different wavelengths suggests the presence of resonance enhancement.
BIOMEDICAL OPTICS EXPRESS
(2023)
Proceedings Paper
Optics
Fei Xia, Monique Gevers, Andreas Fognini, Aaron T. Mok, Bo Li, Najva Akabri, Iman E. Zadeh, Jessie Qin-Dregely, Chris Xu
Summary: By utilizing the advantages of short-wave infrared wavelength, we have demonstrated one-photon fluorescence confocal microscopy of adult mouse brains with penetration depths up to 1.7mm, achieved by labeling quantum dots with 1300 nm excitation and 1700 nm emission and detecting them with a single-photon superconducting nanowire detector.
ADVANCED PHOTON COUNTING TECHNIQUES XVI
(2022)
Proceedings Paper
Engineering, Biomedical
Chris Xu
Summary: Multiphoton microscopy is a crucial technique for high-resolution, deep imaging in scattering biological tissue. An adaptive excitation source has been developed to transform a conventional multiphoton microscope into a random-access microscope, enabling more efficient imaging by exciting only the regions of interest.
HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY VII
(2022)
