期刊
OPTICS LETTERS
卷 37, 期 13, 页码 2565-2567出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.37.002565
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资金
- National Science Foundation [CBET 07-54122]
- National Institutes of Health [R01 GM086382]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [754122] Funding Source: National Science Foundation
A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 mu m x 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications. (C) 2012 Optical Society of America
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