期刊
OPTICS LETTERS
卷 33, 期 24, 页码 2961-2963出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.33.002961
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- Research Grants Council of Hong Kong Special Administrative Region government [GRF2150569]
We report the transfection of human hepatocarcinoma (HepG2) cells by femtosecond (fs) laser pulses at 1554 nm. It was found that HepG2 cells could be perforated transiently to admit propidium iodide by that laser. This photoporation was safe, as the membrane resealed itself within a short time and no mitochondrial depolarization was detected. The. cells were next photoporated in the presence of DNA plasmids for the expression of green fluorescence protein, and about 80% of the exposed cells showed green fluorescence 24 h later. Thus it could be concluded that it is safe and efficient to use fs laser at 1554 ore to transfect foreign molecules into cells under a standard microscope. (C) 2008 Optical Society of America
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