Article
Multidisciplinary Sciences
Daniel U. Campos-Delgado, Omar Gutierrez-Navarro, Ricardo Salinas-Martinez, Elvis Duran, Aldo R. Mejia-Rodriguez, Miguel J. Velazquez-Duran, Javier A. Jo
Summary: The deconvolution process is crucial for quantitative evaluation of FLIM samples, separating the FluoIRs of the sample from the InstR. Two BDE algorithms are introduced based on linear combinations of multi-exponential functions to model each FluoIR with assumed InstR structure. The local and global perspectives of the methodology show consistent convergence responses with least squares formulation and ALS methodology iteratively solving the estimation problems for FluoIRs and InstR.
Article
Biochemical Research Methods
P. Wang, F. Hecht, G. Ossato, S. Tille, S. E. Fraser, J. A. Junge
Summary: Fluorescence lifetime imaging microscopy (FLIM) with phasor analysis allows for easy visualization and analysis of fluorophores' lifetimes, but typically suffers from low photon budgets leading to poor signal to noise ratios. Traditional median filters used in phasor analysis degrade high spatial frequency FLIM information, especially edges and puncta. A new filtering strategy using complex wavelet filtering and Anscombe transform preserves fine structures and accurate lifetimes in photon starved FLIM imaging, outperforming median filters and enabling FLIM imaging with lower laser power and faster imaging speed.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Engineering, Environmental
Adrian Monteleone, Folker Wenzel, Heinz Langhals, Daniel Dietrich
Summary: The FLIM method was tested for identification and characterization of six types of plastics, showing significant differentiation in 94.55% of the comparisons. Results suggest that FLIM has the potential for sub-micrometer plastic characterization and allows for visual 3D-sectioning of samples, which could be important for identifying and characterizing plastics in tissue and environmental samples.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
Gilad Yahav, Shweta Pawar, Yitzchak Weber, Bar Atuar, Hamootal Duadi, Dror Fixler
Summary: Wide-field measurements of time-resolved fluorescence anisotropy (TR-FA) provide valuable information about the rotational mobility of fluorophores and have potential applications in cellular imaging and biochemical sensing. This study extends the existing fluorescence lifetime imaging microscopy (FLIM) to include TR-FA imaging (TR-FAIM), and applies it to the study of CD-gold nano conjugates. The results show significant changes in FLT, r, and. due to the attachment of gold nanoparticles to CDs.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Engineering, Electrical & Electronic
Daniel Belega, Dario Petri
Summary: This article analyzes the Cramer-Rao lower bounds for unbiased estimators of sampled real-valued sine-wave parameters, showing that existing relationships may have significant errors under certain conditions. Approximate yet accurate and simple expressions for the CRLBs are proposed and verified through numerical results or computer simulations.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Biochemical Research Methods
Rupsa Datta, Amani Gillette, Matthew Stefely, Melissa C. Skala
Summary: Fluorescence lifetime imaging microscopy (FLIM) is a powerful molecular imaging technique that measures the decay rate of fluorophores to provide insights into molecular interactions. Major advances in FLIM instrumentation, analysis, and biological and clinical applications over the last year have contributed to the growing interest in FLIM and continuous technological improvements in biomedical research.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Jie Cui, Hailiang Nie, Shunping Zang, Sa Su, Mengxu Gao, Jing Jing, Xiaoling Zhang
Summary: The study introduced a new fluorescent molecular rotor, BODIPY-P, for monitoring viscosity fluctuations within lysosomes during autophagy and apoptosis. By utilizing this rotor, specific mapping and quantifying of lysosomal viscosity fluctuations were successfully achieved through dual mode imaging. BODIPY-P demonstrated high sensitivity and selectivity to viscosity, specific lysosome targeting, quick washing free imaging, and excellent intracellular photostability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Optics
Lukas Klein, Arne S. Kristoffersen, Jan Tous, Karel Zidek
Summary: This article introduces a versatile microscope based on single-pixel imaging that can measure hyperspectral information and fluorescence lifetime imaging simultaneously. The microscope has potential applications in material characterization studies at both macro- and micro-levels.
Article
Chemistry, Physical
Huaxin Zhang, Honglin Wang, Hongmei Yang, Dan Zhou, Qinghua Xia
Summary: Water-soluble amino-terminated SiQDs were successfully synthesized without extra reductants or catalysts, showing excellent fluorescence properties and stability. SiQDs could stably bind with human serum albumin and have potential biomedical applications.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Martin Priessner, Peter A. Summers, Benjamin W. Lewis, Magdalena Sastre, Liming Ying, Marina K. Kuimova, Ramon Vilar
Summary: A new optical probe based on BODIPY has been developed, which exhibits fluorescence intensity switch-on upon binding to copper(I) and can be used to visualize copper(I) pools in lysosomes of live cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Medicine, Research & Experimental
Liping Chen, Guihui Qin, Yuhong Liu, Moxin Li, Yue Li, Lun-Zhang Guo, Lidong Du, Weiming Zheng, Pei-Chun Wu, Yueh-Hsun Chuang, Xiaoyan Wang, Tzung-Dau Wang, Ja-An Annie Ho, Tzu-Ming Liu
Summary: The study identifies distinguishable features of prediabetic adipose tissues through metabolic imaging, providing a potential diagnostic method for prediabetes. In prediabetic adipose tissues, only adipocytes exhibit a low redox ratio of metabolic fluorescence and high free NAD(P)H fraction, which could be a risk indicator of prediabetic insulin resistance.
Article
Environmental Sciences
Fang Zhou, Xin Wang, Guangxin Wang, Yanxia Zuo
Summary: With the increasing use and release of plastic products, microplastics have become a significant concern as they accumulate in the environment. This study proposes a fluorescence lifetime imaging technology for the rapid and accurate detection of microplastics, utilizing phasor analysis to obtain unique phasor fingerprints. The method allows for quick and intuitive identification of different microplastics and their distribution in mixed samples. The results show the potential applications of this method in identifying and analyzing microplastics in complex environments.
Article
Engineering, Biomedical
Ali Taimori, Duncan Humphries, Gareth Williams, Kevin Dhaliwal, Neil Finlayson, James Hopgood
Summary: The paper presents a robust and computationally efficient framework for recovering fluorescence decay signals from photon-count histograms, improving bias by 28% compared to competing methods in low photon-count regimes and processing data around 63 times faster than the gold standard least squares fit on real images.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Vivek Kumar Verma, Prachi Srivastava, Shivesh Sabbarwal, Mamata Singh, Biplob Koch, Manoj Kumar
Summary: White light-emitting gadolinium oxide nanoclusters were synthesized via a one-pot technique, exhibiting tunable emission wavelength, high cell viability, and stability, making them suitable for cell imaging.
