4.6 Article

Spatial light interference tomography (SLIT)

期刊

OPTICS EXPRESS
卷 19, 期 21, 页码 19907-19918

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OPTICAL SOC AMER
DOI: 10.1364/OE.19.019907

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  1. National Science Foundation [08-46660, CBET-1040462, 0843604]
  2. Grainger Foundation
  3. National Institute of Mental Health [R21 MH085220]
  4. National Institute of Child Health and Human Development [HD007333]
  5. Div Of Molecular and Cellular Bioscience
  6. Direct For Biological Sciences [0843604] Funding Source: National Science Foundation

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We present spatial light interference tomography (SLIT), a label-free method for 3D imaging of transparent structures such as live cells. SLIT uses the principle of interferometric imaging with broadband fields and combines the optical gating due to the micron-scale coherence length with that of the high numerical aperture objective lens. Measuring the phase shift map associated with the object as it is translated through focus provides full information about the 3D distribution associated with the refractive index. Using a reconstruction algorithm based on the Born approximation, we show that the sample structure may be recovered via a 3D, complex field deconvolution. We illustrate the method with reconstructed tomographic refractive index distributions of microspheres, photonic crystals, and unstained living cells. (C) 2011 Optical Society of America

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