Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 ( x, y, lambda). The spatial resolution is similar to 0.45 mu m with a FOV of 100 x 100 mu m(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval similar to 3.3 nm. The channel's spectral resolution is similar to 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging. (C) 2010 Optical Society of America