期刊
OPTICS EXPRESS
卷 18, 期 10, 页码 10627-10641出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.18.010627
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资金
- Alexander von Humboldt Foundation
- Biomedical Research Council in Singapore [BMRC 07/1/21/19/488, R-143-000-351-305]
- Singapore MIT Alliance
The life sciences require new highly sensitive imaging tools, which allow the quantitative measurement of molecular parameters within a physiological three-dimensional (3D) environment. Therefore, we combined single plane illumination microscopy (SPIM) with camera based fluorescence correlation spectroscopy (FCS). SPIM-FCS provides contiguous particle number and diffusion coefficient images with a high spatial resolution in homo-and heterogeneous 3D specimens and live zebrafish embryos. Our SPIM-FCS recorded up to 4096 spectra within 56 seconds at a laser power of 60 mu W without damaging the embryo. This new FCS modality provides more measurements per time and more, less photo-toxic measurements per sample than confocal based methods. In essence, SPIM-FCS offers new opportunities to observe biomolecular interactions quantitatively and functions in a highly multiplexed manner within a physiologically relevant 3D environment. (C) 2010 Optical Society of America
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