Review
Chemistry, Analytical
Vinayak Juvekar, Hyo Won Lee, Dong Joon Lee, Hwan Myung Kim
Summary: Two-photon microscopy is an important tool for deep-tissue imaging, providing high-resolution fluorescence images beyond millimeter depths. Recent research has focused on developing innovative strategies and probes for quantitative analysis using two-photon fluorescence.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Vinayak Juvekar, Sang Jun Park, Juyoung Yoon, Hwan Myung Kim
Summary: Two-photon microscopy combined with two-photon fluorescent probes enables visualization of metal ion distribution in live tissues, facilitating the development of probes for metal ion detection. Recent progress between 2014 and 2020, including design strategies, history, and specific applications, was summarized in this review.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Conghao Wang, Qinmiao Chen, Huilan Liu, Runlong Wu, Xiong Jiang, Qiang Fu, Zhe Zhao, Ye Zhao, Yuqian Gao, Bosong Yu, Hongchen Jiao, Aimin Wang, Shumin Xiao, Lishuang Feng
Summary: Miniature two-photon microscopy is a powerful technique for investigating brain activity in freely moving animals. This study successfully addressed the challenges of probe weight and animal behavior constraints by employing metalenses, and demonstrated two-photon imaging of mouse brain structures.
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Review
Chemistry, Multidisciplinary
Luqman Khan, Rick van Lanen, Govert Hoogland, Olaf Schijns, Kim Rijkers, Dimitrios Kapsokalyvas, Marc van Zandvoort, Roel Haeren
Summary: The review highlighted the application of two-photon imaging (2PI) in epilepsy research, showcasing its ability to evaluate pathomechanisms associated with epileptic seizures, including propagation models, cellular changes, and neurovascular alterations. The findings suggest that 2PI is a valuable imaging technique in advancing our understanding of epilepsy pathophysiology.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
M. Marini, A. Nardini, R. Martinez Vazquez, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, M. T. Raimondi, G. Chirico
Summary: Non-linear excitation microscopy has advantages over conventional confocal techniques for in-vivo imaging, but tissue penetration can still be problematic. This study develops and tests fibroblast cell culture plano-convex microlenses for non-linear imaging of biological tissue. The microlenses can be used individually or in an array, and they provide magnified fluorescence images without substantially affecting the signal-to-noise ratio. These results pave the way for optical in-vivo inspection of biological processes using implanted micro-optics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemical Research Methods
Po-Yen Lin, Sheng-Ping L. Hwang, Chi-Hon Lee, Bi-Chang Chen
Summary: By incorporating axicon imaging into LSFM, the depth of field is extended, allowing for faster three-dimensional imaging and capturing dynamic changes in physiological processes.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Analytical
Sang Jun Park, Myoung Ki Cho, Dong Joon Lee, Deuk Hwa Song, Chang Su Lim, Choong-Kyun Noh, Dong Min Lee, Kyeong Sook Choi, Sung Jae Shin, Hwan Myung Kim
Summary: A two-photon probe SCX was developed to reflect the level of COX-2 in colorectal cancer patients and distinguish between cancerous and normal tissue. In vitro and cell imaging studies showed that SCX could effectively differentiate the expression levels of COX-2 in cancerous and normal cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Weijian Zong, Runlong Wu, Shiyuan Chen, Junjie Wu, Hanbin Wang, Zhe Zhao, Guoqing Chen, Rui Tu, Danlei Wu, Yanhui Hu, Yangyang Xu, Yao Wang, Zhuoli Duan, Haitao Wu, Yunfeng Zhang, Jue Zhang, Aimin Wang, Liangyi Chen, Heping Cheng
Summary: Our miniature two-photon microscope, equipped with an axial scanning mechanism and a long-working-distance miniature objective, enables multi-plane imaging within a small animal's body, aiding in deciphering neuronal mechanisms.
Editorial Material
Biochemical Research Methods
Jerome A. Lecoq, Roman Boehringer, Benjamin F. Grewe
Summary: New three-photon miniature microscopes enable the study of neuronal networks in the deep regions of the brains of behaving animals.
Article
Multidisciplinary Sciences
Giovanni Barbera, Rachel Jun, Yan Zhang, Bo Liang, Yun Li, Da-Ting Lin
Summary: In this work, a lightweight one-photon microscope capable of imaging at different focal depths is presented. The focal plane can be dynamically changed by modulating the pulse width of the control signal, enabling the switching of focal plane between frames. Experimental results showed an increase in the number of imaged neurons compared to single plane imaging.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Asylkhan Rakhymzhan, Andreas Acs, Anja E. Hauser, Thomas H. Winkler, Raluca A. Niesner
Summary: Two-photon microscopy is widely used for monitoring cellular dynamics and communication in complex systems, particularly in understanding the immune system's cellular interactions and pathophysiologic processes in vivo. Simultaneous multiplexed imaging is necessary to comprehend the dynamic orchestration of multiple cellular and non-cellular tissue compartments defining immune responses. By improving the similarity spectral unmixing algorithm, probes can be more accurately assigned to tissue compartments labeled by single fluorophores with similar spectral fingerprints.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Yoko Mizuta
Summary: Two-photon excitation microscopy (2PEM) allows deep imaging of living tissues with reduced autofluorescence compared to confocal microscopy, achieving higher resolution. Fluorescent proteins with long emission wavelengths, such as orange-red fluorescent proteins, are suitable for high-quality two-photon live imaging in plants.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Bowen Wei, Chenmao Wang, Zongyue Cheng, Baoling Lai, Wen-Biao Gan, Meng Cui
Summary: COMPACT allows imaging of large volumes in the brain through a periscope-like probe inside a glass capillary, enabling observation of the dynamics of neuronal structure and function in different regions. This technology provides a valuable tool for understanding the neural circuit mechanisms underlying behavior.
Article
Endocrinology & Metabolism
Tadayuki Yoshitake, Seymour Rosen, Lucas C. Cahill, Simon Lamothe, Ashley Ward, James G. Fujimoto
Summary: Nonlinear microscopy (NLM) is a promising technique for rapid evaluation of bone pathology. It enables visualization of normal bone architecture, articular cartilage, and pathological bone features, but training is required for accurate evaluation. Further studies are needed to assess diagnostic performance and address evaluation challenges with NLM.
Article
Physics, Applied
Meiqi Li, Yaning Li, Wenhui Liu, Amit Lal, Shan Jiang, Dayong Jin, Houpu Yang, Shu Wang, Karl Zhanghao, Peng Xi
APPLIED PHYSICS LETTERS
(2020)
Review
Anatomy & Morphology
Zhaoyang Wu, Xinzhu Xu, Peng Xi
Summary: STED microscopy allows high lateral and axial resolution, long term imaging in living cells. This review discusses recent technical advances in STED microscopy, emphasizing on XYZt four dimensions resolution and measurement range. Different STED technical advances and novel STED probes are explored for their respective application in biological subcellular imaging, serving as a practical guide for choosing a suitable approach to advanced STED super-resolution imaging.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Chemistry, Multidisciplinary
Zhongwei Man, Hongtu Cui, Zheng Lv, Zhenzhen Xu, Zhaoyang Wu, Yishi Wu, Qing Liao, Meihui Liu, Peng Xi, Lemin Zheng, Hongbing Fu
Summary: This study achieves ultra-low power STED nanoscopy with highly emissive nanoparticles, enabling high-resolution imaging in living cells. The method has high excitation efficiency and cell viability protection.
Article
Physics, Applied
Hongjin Li, Zihan Wu, Zhichao Yang, Karl Zhanghao, Peng Xi, Dayong Jin
Summary: A multi-focus scheme was developed to extend the Field of View (FoV) of light sheet fluorescence microscopy. Axially overlapped Gaussian light sheets significantly enlarged the FoV with improved uniformity and negligible loss in axial resolution. The multi-focus Gaussian light sheet displayed significantly improved optical sectioning ability over the full FoV when imaging cells and zebrafish compared to the conventional single-focus one.
APPLIED PHYSICS LETTERS
(2021)
Article
Anatomy & Morphology
Xinzhu Xu, Kun Zhao, Wei Ren, Zhaoyang Wu, Wentao Yu, Chendi Shao, Kebin Shi, Peng Xi
Summary: STED microscopy achieves super-resolution in biological imaging beyond the diffraction limit. This study presents a concise protocol for constructing a dual-pulse STED setup using a super-continuum laser, and introduces a flexible and dismountable Bessel modulation module for potential 2D-stack STED imaging. Experiments, notices, and discussions on important check-points, such as detector saturation, are provided, with validation of the system's functionality in the results.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Optics
Jianwei Chen, Benxi Yao, Zhichao Yang, Wei Shi, Tingdan Luo, Peng Xi, Dayong Jin, Yiming Li
Summary: In this study, a new ratiometric multicolor imaging strategy for 4Pi-SMLM was developed, achieving optimal 3D resolution and color separation.
Article
Anatomy & Morphology
Yiming Wang, Meiling Guan, Yan Zhang, Karl Zhanghao, Peng Xi
Summary: Glucose triggers the plasticity of axon initial segment (AIS) in cultured neurons. Structured illumination microscopy imaging reveals an increased spacing in the AIS lattice structure under glucose treatment. The 480-kDa AnkG plays a crucial role in AIS plasticity.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Editorial Material
Optics
Peng Xi, Xunbin Wei, Junle Qu, Valery V. Tuchin
LIGHT-SCIENCE & APPLICATIONS
(2022)
Editorial Material
Optics
Xinzhu Xu, Peng Xi
Summary: The cascade amplified depletion effect in lanthanide upconversion systems has a significant impact on their emission bands, allowing for multi-color nanoscopy with only one pair of low-power NIR CW lasers.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Editorial Material
Optics
Xinzhu Xu, Shu Jia, Peng Xi
Summary: This study introduces a new method that simplifies the conventional MINFLUX process in scanning fluorescence microscopy by using a donut excitation that moves around a single molecule. The method achieves localization precision and resolution close to those of conventional MINFLUX and plays a crucial role in reconstructing localization using maximum likelihood estimation. The proposed raster-scanning MINFLUX can inspire researchers in STED or confocal setup to quickly switch to MINFLUX and further explore its applications in bio-specimens or optics.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Optics
Chao He, Jianyu Lin, Jintao Chang, Jacopo Antonello, Ben Dai, Jingyu Wang, Jiahe Cui, Ji Qi, Min Wu, Daniel S. Elson, Peng Xi, Andrew Forbes, Martin J. Booth
Summary: Traditional approaches to polarization sensing are limited, while this study proposes a measurement paradigm that maps all polarization analyzer states into a single vectorially structured light field using a universal Poincare generator, providing a single-step sensing procedure.
Editorial Material
Optics
Xin Chen, Yiwei Hou, Peng Xi
Summary: Principal component analysis (PCA), a common dimensionality reduction method, is used in SIM to accurately identify the frequency vectors and pattern phases of the illumination pattern. It offers precise subpixel accuracy, fast speed, and noise-robustness, making it promising for real-time and long-term live-cell imaging.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Review
Optics
Xin Chen, Suyi Zhong, Yiwei Hou, Ruijie Cao, Wenyi Wang, Dong Li, Qionghai Dai, Donghyun Kim, Peng Xi
Summary: Structured illumination microscopy (SIM) has become the standard for next-generation wide-field microscopy, offering ultrahigh imaging speed, superresolution, a large field-of-view, and long-term imaging. Advanced reconstruction algorithms are necessary to unlock the full potential of SIM system hardware. This article introduces the theory and implementation modalities of two SIM algorithms, optical sectioning SIM (OS-SIM) and superresolution SIM (SR-SIM), and provides an overview of existing processing algorithms and the development of reconstruction algorithms for different SIM techniques. Comparison of off-the-shelf SIM systems and future perspectives are also discussed.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Correction
Engineering, Electrical & Electronic
Amit Lal, Chunyan Sha, Peng Xi
Summary: This article presents corrections to the paper "Structured Illumination Microscopy Image Reconstruction Algorithm".
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Jing Sun, Xichuan Ge, Boya Jin, Shiyi Li, Yiwei Hou, Suyi Zhong, Zikang Yang, Peng Xi, Meiqi Li, Baoxiang Gao
Summary: Mitochondrial probe SiRPFA with high lipophilicity and mitochondrial membrane potential independent properties allows for clear visualization of changes in mitochondrial cristae morphology during autophagy induced by starvation or apoptosis using stimulated emission depletion microscopy.
CHEMICAL COMMUNICATIONS
(2023)
Article
Optics
Hang Dong, Zhixin Sun, Jingyi Li, Yahui Li, Wei Zhang, Guangyong Jin
Summary: This paper calculates thermal stresses and adsorption forces to determine laser cleaning conditions and establishes relevant models. Experimental results show that the removal effect is better with increasing nanosecond pulse delay, with the best effect achieved at 600 milliseconds pulse delay. Based on the findings, the mechanisms of oxide film removal involve thermal stress against adsorption and plasma shock wave breaking the oxide layer.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Junjie Zhang, Wenjun Li, Bingtao Li, Zheng-Da Hu, Jicheng Wang, Feng Zhang, Lei Wang
Summary: A multilayer thin film device structure based on Tamm plasmons is proposed for high-performance near-infrared hot electron photodetectors. By optimizing the device structure parameters, high responsivity detection can be achieved.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Hong Huang, Zhiguang Han
Summary: This paper proposes a new ghost imaging reconstruction method using ordered orthogonal Hadamard derived speckle as the illumination speckle series, and introduces the alternating direction multiplier method to improve the imaging performance. The evaluation results show that the method can achieve high-quality reconstructed images under low sampling conditions.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Moritsugu Sakamoto, Yuki Ono, Kohei Noda, Tomoyuki Sasaki, Nobuhiro Kawatsuki, Masayuki Tanaka, Hiroshi Ono
Summary: The effect of wavelength and polarization of illuminating light in polarization imaging for birefringent objects placed behind a scattering structure was experimentally investigated. The result shows that the spatial distribution of the birefringent object was more clearly visualized in the longer wavelength combined with circularly polarized light illumination. This finding indicates the potential of using polarization imaging with circularly polarized light illumination in the near-infrared range for visualizing birefringent objects with scattering.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Peihui Du, Hongfang Wang, Pengwei Li, Rukeyemuhan Abadula, Hmbat Batelbek, Min Gao
Summary: In this study, we theoretically demonstrate the strong coupling between Tamm plasmons and exciton polaritons in metal Al/DBR-molecular structures, extending the operating wavelength to the deep ultraviolet region. The coupling strength can be effectively manipulated by adjusting the structure parameters, offering potential benefits for the development of new-style optical filters.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Priyanka Chaudhary, Akhilesh Kumar Mishra
Summary: We design and numerically investigate the switching dynamics between two outer waveguides in a parity-time (PT)-symmetric adiabatically coupled three waveguides nonlinear directional coupler (NLDC) system. The study shows that the device can provide switching even when the middle waveguide is nonlinear and the outer waveguides are linear. Furthermore, the effect of loss to gain ratio on critical switching power and the impact of launched light power and gain (loss) value on transmitted power are also studied.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Wei Feng, Yongcong Yi, Shuyang Li, Zhi Xiong, Boya Xie, Zhen Zeng
Summary: Traditional imaging techniques are ineffective in achieving clear underwater imaging due to the presence of scattering media. Single-pixel imaging (SPI) system based on Unet++ offers a solution for reconstructing high-quality images in highly turbid water environments.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Xiaorui Qu, Jufeng Zhao, Haijun Tian, Junjie Zhu, Guangmang Cui
Summary: This paper studies the structural similarity between RGB and spectral images and proposes a non-iterative Images Structure Similarity (ISS) method for fast reconstruction of spectral images. Additionally, the input of the Deep Image Prior (DIP) method is optimized for the first time by using the initial spectral data reconstructed by ISS, leading to an improved starting value for the iteration. The experimental results show that the proposed method can enhance the reconstruction quality in both spectral and spatial resolutions, while significantly reducing the reconstruction time compared to other DIP-based methods.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Donghe Sheng, Zhe Han, Zanyang Qiao, Tianpei Dong, Chenxi Wang, Huiping Tian
Summary: In this study, a distributed multi-parameter sensor based on an etched few-mode multi-core fiber is proposed, allowing simultaneous sensing of temperature, strain, and sample refractive index. By combining space division multiplexing and stimulated Brillouin scattering, the sensor achieves high sensitivity in detecting these parameters.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Dehao Chen, Zhenwu Mo, Zehong Liang, Junjie Jiang, Huilin Tang, Yidan Sun, Ziyu Wang, Quanfeng Wei, Yanru Chen, Dongmei Deng
Summary: In this study, a novel family of elliptical Airyprime vortex beams (EAPVBs) is introduced, which inherits the excellent self-focusing properties of the circular Airyprime vortex beam (CAPVB). The asymmetric focusing of EAPVB leads to some novel properties, such as the splitting of high-order optical vortex and the formation of two foci. By taking advantage of these properties, EAPVB is constructed as a tunable optical bottle for particle capture.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Xiao Ma, Qiongchan Shao, Jian-Jun He
Summary: In this study, an SHS chip based on Su8 waveguide was designed and fabricated. By physically adjusting the metal electrodes and compensating for transmissivity fluctuations, the generation of side ripples was successfully suppressed.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Hongbin Zhang, Jiansen Du, Zongtao Chi, Hailin Cong, Bin Wang
Summary: In this paper, a novel type of dual-wavelength confocal metalens is proposed to solve the spatial crosstalk between two wavelengths. The metalens can greatly reduce the spatial crosstalk and achieve high precision and efficiency in confocal imaging. It can also focus light in specific wavelength ranges, making it suitable for imaging, microscopy, and optical fiber communication.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Rui Qiu, Guanmao Zhang, Shaokai Du, Jie Liu, Hongyu Jib, Kaiyun Bi, Bochuan Xing, Guangchao Diao
Summary: Recent research has developed an achromatic metalens that shows potential for replacing traditional lenses. This study focuses on a continuously variable focus height broadband achromatic metalens for long-wavelength infrared applications. By optimizing materials and parameters, chromatic aberration is effectively corrected, making it suitable for high-resolution LWIR imaging and spectroscopy systems.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Marcos Troncoso-Costas, Gaurav Jain, Yiming Li, Mohammed Patel, Lakshmi Narayanan Venkatasubramani, Sean O'Duill, Frank Smyth, Andrew Ellis, Francisco Diaz-Otero, Colm Browning, Liam Barry
Summary: In this work, a fast-switching tuneable laser capable of wide wavelength coverage, low noise and linewidth levels suitable for high-order modulation formats is demonstrated. The laser is characterized to cover a wavelength range of 35 nm in the C-band with nanosecond switching time. It is used to successfully demonstrate 480 Gbit/s 16QAM transmission over 25 km of single-mode fiber for a wavelength range of 19 nm.
OPTICS COMMUNICATIONS
(2024)
Article
Optics
Takeshi Moriyasu, Masahiko Tani, Hideaki Kitahara, Takashi Furuya, Jessica Afalla, Toshiro Kohmoto, Daishiro Koide, Hiroki Sato, Mitsutaka Kumakura
Summary: Optical pump-terahertz probe spectroscopy was used to study the photocarrier dynamics and optical characteristics of semiconductor Si. The results showed that the thickness of Si influenced the transmitted terahertz field amplitude and peak delay time, indicating differences in photocarrier dynamics between different Si materials.
OPTICS COMMUNICATIONS
(2024)
