期刊
ONCOLOGY REPORTS
卷 32, 期 3, 页码 1037-1042出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2014.3312
关键词
hepatocellular carcinoma; Ligustrum lucidum fruit; apoptosis; cell senescence; p21
类别
资金
- National Natural Science Foundation of China [81273726]
- Three-year Action Program of Shanghai Municipality for Traditional Chinese Medicine [ZYSNXD-CC-ZDYJ017]
- Xing-Lin Scholars Program
- Long-Yi Scholars and Research Team Program from the State Clinical Research Center of TCM in Longhua Hospital [LYTD-04]
- Shanghai Shen Kang Platform Grant [SHDC12007206]
Nu-zhen-zi, the fruit of Ligustrum lucidum Ait., is one of the most frequently used liver Yin tonifying Chinese herbs for the treatment of liver cancer. However, the effect of Ligustrum lucidum fruit on hepatocarcinoma cells remains unknown. In the present study, we evaluated the effects of a Ligustrum lucidum fruit extract (LLFE) on human hepatocellular carcinoma Bel-7402 cells. The results showed that LLFE inhibited the proliferation of the Bel-7402 cells in a dose- and time-dependent manner. LLFE induced apoptosis in Bel-7402 cells accompanied by activation of caspase-3, -8 and -9. LLFE-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. LLFE treatment also caused a large and fiat morphologic cellular change, positive SA-beta-gal staining, and G(0)/G(1) phase cell cycle arrest in the Bel-7402 cells, accompanied by upregulation of p21 and downregulation of RB phosphorylation. Specific knockdown of p21 expression by RNA interference partially abrogated LLFE-induced apoptosis, and significantly abrogated LLFE-induced cell senescence. These observations suggest that Nu-zhen-zi is a potential anticancer herb and support the traditional use of NU-zhen-zi for hepatocarcinoma treatment.
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