Article
Cell Biology
Jyotirmaya Behera, Kimberly E. Kelly, Neetu Tyagi
Summary: The study found that hydrogen sulfide can protect against ethanol-induced endothelial permeability by affecting miR-218, thus reducing damage to endothelial cells under the influence of alcohol.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Hao Ning, Lu Zhang, Baicheng Zhu, Xinxin Zhou, Tianyuan Zhang, Teng Ma
Summary: Breakdown of the blood-brain barrier (BBB) is an important pathological marker in the development of Alzheimer's disease. Beta-amyloid deposition disrupts tight junction proteins formed by endothelial cells, leading to increased BBB permeability. Through regulation of various genes and molecules, the TARBP2/SNHG7/miR-17-5p/NFATC3 pathway may provide insights into the changes in the BBB during the progression of Alzheimer's disease.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Matthew Dyer, Quy Xiao Xuan Lin, Sofiia Shapoval, Denis Thieffry, Touati Benoukraf
Summary: MethMotif is a publicly available database that provides a comprehensive repository of transcription factor-binding profiles with DNA methylation patterns. The latest release includes over 700 position weight matrices, segregated based on their cofactors and DNA methylation status. The database also offers precomputed GO annotations for human TFs and TF-co-TF complexes, allowing for a comprehensive analysis of TF functions in their context with cofactors. Furthermore, MethMotif has been expanded to include data for two additional species, increasing its applicability and value to the scientific community.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Yanqing Wu, Peng Duan, Yujiao Wen, Jin Zhang, Xiaoli Wang, Juan Dong, Qiang Zhao, Shenglei Feng, Chunyu Lv, Yang Guo, Satoshi H. Namekawa, Shuiqiao Yuan
Summary: This study reveals the importance of UHRF1 in the epigenetic regulation of Sertoli cells during spermatogenesis. UHRF1 regulates the expression of extracellular matrix-related genes and establishes the crosstalk between Sertoli cells and germ cells.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Haiyang Yang, Xueyan Li, Gang Li, Huating Huang, Wenning Yang, Xiaoquan Jiang, Muli Sen, Jingjing Liu, Yang Liu, Yanli Pan, Guopeng Wang
Summary: This study emphasizes the importance of accurate quantitative determination of affinity and binding kinetics for tight binding inhibition. Recommendations on experimental design and mathematical calculation approaches are provided to evaluate affinity and BK parameters for inhibitors against xanthine oxidase. Significant differences in results and the influence of different data analytical methods on enzyme engagement in human are discussed.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
L. Liu, Y. Li, Y. He, Z. Wang, H. Zhao, X. Jin, D. Shi, X. Wang
Summary: The study demonstrated that HDRsEf1 could protect IPEC-J2 cells against LPS-induced downregulation of ZO-1 expression by inhibiting the TLR2/4-mediated JNK-AP-1 signaling cascade and TNF-alpha production. This provides a theoretical basis for probiotics to regulate the expression of intestinal tight junction proteins and offers technical support for probiotics in preventing and treating animal intestinal infectious diseases.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Cell Biology
Diana Schuhmacher, Jean-Marie Sontag, Estelle Sontag
Summary: The study reveals the crucial roles of LCMT1, PME1, and PP2A methylation/demethylation in modulating TJ assembly and functional integrity, positioning PP2A at the interface of one-carbon metabolism and the regulation of key TJ and polarity proteins that are deregulated in many human diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinmyeong Kim, Seung Woo Noh, Chulhwan Park, Jin-Ho Lee, Hyeon-Yeol Cho, Junhong Min, Taek Lee
Summary: This study presents a label-free electrochemical biosensor for the effective detection of TNF-alpha in human serum, offering a good dynamic range and sensitivity.
BIOELECTROCHEMISTRY
(2021)
Article
Toxicology
Enkai Li, Chuang Li, Nathan Horn, Kolapo M. Ajuwon
Summary: The role of peroxisome proliferator activated receptor gamma (PPAR gamma) in the regulation of adipocyte differentiation has been well characterized. This study aimed to understand the role of PPAR gamma activation in the regulation of intestinal barrier function. The results showed that PPAR gamma activation increased the protein expression of tight junction proteins, claudin-3 and 4, and improved intestinal barrier function. Therefore, PPAR gamma agonists could be effective in preventing DON-induced gut barrier dysfunction.
TOXICOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Diego Hernandez-Saavedra, Laura Moody, Xinyu Tang, Zachary J. Goldberg, Alex P. Wang, Hong Chen, Yuan-Xiang Pan
Summary: Chronic caloric restriction (CR) was found to significantly reduce muscle inflammation and TNF levels, while affecting cells' methylation status and regulating transcriptional and post-transcriptional regulation. This suggests that chronic CR can regulate muscle inflammation through multiple pathways.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Hematology
Shelby L. Sloan, Fiona Brown, Mackenzie Long, Christoph Weigel, Shirsha Koirala, Ji-Hyun Chung, Betsy Pray, Lynda Villagomez, Claire Hinterschied, Anuvrat Sircar, Jobeth Helmig-Mason, Alexander Prouty, Eric Brooks, Youssef Youssef, Walter Hanel, Samir Parekh, Wing Keung Chan, Zhengming Chen, Rosa Lapalombella, Lalit Sehgal, Kris Vaddi, Peggy Scherle, Selina Chen-Kiang, Maurizio Di Liberto, Olivier Elemento, Cem Meydan, Jonathan Foox, Daniel Butler, Christopher E. Mason, Robert A. Baiocchi, Lapo Alinari
Summary: Inhibition of PRMT5 in mantle cell lymphoma demonstrated antitumor activity and restored regulatory activity of the cell cycle, apoptotic cell death, and negative regulators of the B-cell receptor-PI3K/AKT signaling pathway. The selective targeting of PRMT5 shows promise as a potential therapy for patients with relapsed/refractory MCL.
Review
Oncology
Jiaxin Liu, Jia-nan Li, Hongyu Wu, Panpan Liu
Summary: Epigenetic regulation plays a crucial role in lymphoma development and is closely associated with various malignancies. Understanding these epigenetic mechanisms can guide the development of clinical treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Physical
Felipe S. Stefanello, Yuri G. Kappenberg, Alex Ketzer, Steffany Z. Franceschini, Paulo R. S. Salbego, Thiago Acunha, Pablo A. Nogara, Joao B. T. Rocha, Marcos A. P. Martins, Nilo Zanatta, Bernardo A. Iglesias, Helio G. Bonacorso
Summary: This study synthesized and characterized a series of new SCTz derivatives, investigated their binding properties to DNA and HSA, and found strong binding forces to CT-DNA. UV-Vis and X-ray diffraction analyses revealed a static interaction between the derivatives and DNA, as well as the formation of stable complexes with DNA and HSA.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Clinical Neurology
Qin Ma, Jorge R. Oksenberg, Alessandro Didonna
Summary: This study found that ATXN1 undergoes hypo-methylation at four distinct regions exclusively in B cells of multiple sclerosis patients, and these differentially methylated sites overlap with other regulatory epigenetic marks and MS risk variants. Luciferase assays showed that the loss of methylation leads to an increase in ATXN1 expression. The findings provide insights into ataxin-1 regulation in the immune system and the molecular mechanisms underlying MS risk.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2022)
Article
Oncology
Zhiding Wang, Wei Guan, Mengzhen Wang, Jinghong Chen, Linlin Zhang, Yang Xiao, Lixin Wang, Yonghui Li, Li Yu
Summary: This study reveals a novel mechanism by which AML1-ETO can inhibit AML immune escape by regulating CD48 expression, providing new insights into the role of AML1-ETO and CD48 in AML. The findings suggest that AML patients with AML1-ETO oncogene infusion may have better clinical outcomes.
LEUKEMIA & LYMPHOMA
(2021)
Article
Medicine, Research & Experimental
Caixia Han, Shujiao He, Ruiqi Wang, Xuefeng Gao, Hong Wang, Jingqiao Qiao, Xiangyu Meng, Yonghui Li, Li Yu
Summary: The study revealed that overexpression of ARHGAP9 is associated with poor overall survival in AML patients and can serve as a prognostic biomarker. AML patients with ARHGAP9 overexpression may benefit from autologous or allogeneic hematopoietic stem cell transplantation rather than chemotherapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Hong Wang, Yan Li, Qingyu Xu, Wei Zhou, Chengliang Yin, Ruiqi Wang, Mengzhen Wang, Yuanyuan Xu, Yonghui Li, Li Yu
Summary: The study suggests that upfront transplantation and pretransplant cytoreduction yield similar outcomes for patients with advanced MDS, but achieving remission before transplantation leads to better survival. This highlights the importance of timely transplantation and appropriate pretransplant therapy during the donor search.
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2021)
Article
Oncology
Mengzhen Wang, Ruiqi Wang, Hong Wang, Chongjian Chen, Jiayue Qin, Xiaoning Gao, Li Yu
Summary: This study analyzed the genetic mutation profile of previously untreated acute myeloid leukemia (AML) patients and compared the gene mutation spectrum of refractory/relapsed (R/R) AML patients to newly diagnosed ones. The findings show increased frequencies of tumor suppressor mutations in relapsed AML, and decreased mutation frequencies in specific genes. FLT3-ITD mutation predicted poorer outcomes in terms of complete remission and overall survival in refractory AML and relapsed patients.
LEUKEMIA & LYMPHOMA
(2021)
Article
Biochemistry & Molecular Biology
Ze Chen, Yang-Liu Shao, Li-Li Wang, Ji Lin, Ji-Bin Zhang, Yi Ding, Bin-bin Gao, Dai-Hong Liu, Xiao-Ning Gao
Summary: YTHDF2 is highly expressed in t(8;21) AML patients, associated with a higher risk of relapse and inferior relapse-free survival. Knockdown of YTHDF2 impairs cell proliferation rate, possibly by modulating global m(6)A methylation to promote growth of t(8;21) AML cells.
Review
Immunology
Zhiding Wang, Jinghong Chen, Mengzhen Wang, Linlin Zhang, Li Yu
Summary: Tim-3 serves as an immune checkpoint in AML, playing a critical role in immune responses and elimination of leukemia stem cells. Its dual functionality as a potential target for curing AML sheds new light on AML immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Caixia Han, Xuefeng Gao, Yonghui Li, Juan Zhang, Erna Yang, Li Zhang, Li Yu
Summary: Gene mutations, especially in genes encoding cohesin subunits, play a significant role in the pathogenesis and progression of AML. Despite ongoing research on cohesin mutations in AML, their precise role in clonal evolution and leukemogenesis remains unclear.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Wei Guan, Lei Zhou, Yan Li, Erna Yang, Yangyang Liu, Na Lv, Lin Fu, Yi Ding, Nan Wang, Nan Fang, Qian Liu, Binan Wang, Fuwei Li, Juan Zhang, Maoquan Wang, Lili Wang, Yu Jing, Yonghui Li, Li Yu
Summary: The study found that FLT3-ITD is a poor prognostic factor in AML patients, while the prognostic significance of FLT3-TKD is controversial. Patients with FLT3-ITD may have concurrent mutations in NPM1 and DNMT3A, while CEBPA mutations are less common. MLL and NUP98 are common fusion genes in FLT3-ITD patients, while AML1-ETO and MLL are common fusion genes in FLT3-TKD patients.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2021)
Letter
Oncology
Erna Yang, Desheng Gong, Wei Guan, Jieying Li, Xuefeng Gao, Yonghui Li, Li Yu
Summary: The DNA methylation level significantly increases when the percentage of AML blasts reaches >= 40%, indicating that an accurate DNA methylation level in cancer cells can be obtained when the bone marrow samples of AML patients have more than 40% myeloblasts.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2021)
Review
Oncology
Qingyu Xu, Shujiao He, Li Yu
Summary: FLT3 inhibitors showed improved complete remission in untreated and refractory and relapsed FLT3(+) AML, followed by enhanced survival. Allogeneic hematopoietic stem cell transplantation improved survival in FLT3(+) AML, further prolonged by FLT3 inhibitors after transplantation. Gilteritinib demonstrated the highest probability for improved prognosis in network meta-analysis.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
Juan Zhang, Xuefeng Gao, Li Yu
Summary: Accurate orchestration of gene expression is critical for normal hematopoiesis, with epigenetic aberration being a major cause of AML. HDACs play pivotal roles in chromatin remodeling, regulating stemness maintenance, cell fate determination, proliferation, and differentiation, with potential implications in carcinogenesis. Targeting the interactions between HDACs and oncogenic proteins could provide insights for AML treatment strategies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Erna Yang, Wei Guan, Desheng Gong, Jieying Li, Caixia Han, Juan Zhang, Hong Wang, Synat Kang, Xuefeng Gao, Yonghui Li, Li Yu
Summary: The RUNX1-RUNX1T1 fusion protein in t(8;21) acute myeloid leukemia inhibits a tumor suppressor protein by recruiting DNA-binding proteins to introduce chemical modifications to the UBXN8 gene. Blocking these modifications with drugs can arrest tumor cell division. Increasing UBXN8 expression may offer a promising therapeutic approach for t(8;21) AML.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Review
Oncology
Hong Wang, Yan Li, Wei Zhou, Ruiqi Wang, Yonghui Li, Li Yu
Summary: For patients with MDS, timely transplantation is crucial, and pre-transplant cytoreductive therapy can be used during the search for donors to improve transplant outcomes.
Article
Oncology
Yang Xiao, Jinghong Chen, Jia Wang, Wei Guan, Mengzhen Wang, Linlin Zhang, Zhiding Wang, Lixin Wang, Li Yu
Summary: In patients with AML, the expression of ICAM-1 is silenced, but the hypomethylating agent can upregulate its expression, facilitating NK cells to kill AML cells. High expression of ICAM-1 can reverse AML immune evasion and activate NK cell function, suggesting a new strategy for AML treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Hematology
Li Zhang, Guangyi Jin, Ziren Chen, Changhua Yu, Yonghui Li, Yisheng Li, Jinghong Chen, Li Yu
Summary: Using WT1 CAR-T cells, we demonstrated that LEN enhances CAR-T cell function in a concentration-dependent manner. Our data showed that LEN increased the infiltration of tumors with CD3(+) and CD8(+) T cells, thereby improving the anti-tumor activity of CAR-T cells in vivo. Proteomics studies supported that LEN enhanced the efficacy of CAR-T cells, including T-cell activation, mitochondrial activity, and immune synapse formation.
