期刊
ONCOLOGY REPORTS
卷 29, 期 6, 页码 2297-2302出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2400
关键词
endometrial endometrioid carcinoma; miR-205; ESRRG; proliferation; invasion
类别
资金
- National Natural Science Foundation of China [30872760, 81072139]
Increasing evidence suggests that miR-205 is frequently dysregulated in many types of human cancers, suggesting its important roles in the initiation and progression of cancer. However, the functions of miR-205 in human endometrial endometrioid carcinoma (EEC) are still unknown. In this study, we investigated the expression of miR-205 in both normal endometrium and EEC tissues using TaqMan PCR. Compared to normal tissues, miR-205 was significantly upregulated in EEC (P<0.001). After transfection of miR-205 inhibitors into Ishikawa cells (or transfection of miR-205 mimics into AN3CA cells), we demonstrated that the cellular proliferation, migration and invasion properties were negatively regulated by miR-205. Moreover, by combination of microRNA target prediction algorithms and luciferase reporter system, we identified estrogen-related receptor-gamma (ESRRG) as a target of miR-205. In conclusion, we demonstrated frequent upregulation of miR-205 in EEC. In gain-of-function and loss-of-function assays, inhibition of miR-205 reduced cellular proliferation, migration and invasion; vice versa, increased levels of miR-205 led to upregulated cellular proliferation, migration and invasion. Nonetheless, we identified the ESRRG gene to be a novel target, which could be helpful to elucidate mechanisms underlying the tumorigenesis of EEC.
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