Article
Multidisciplinary Sciences
Amy E. Loats, Samantha Carrera, Anna F. Fleming, Abigail R. E. Roberts, Alice Sherrard, Eneda Toska, Alexander J. Moorhouse, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The study reveals that the lipidated protein BASP1 recruits cholesterol to the promoter region of target genes in the cell nucleus through a conserved interaction motif, and that BASP1's interaction with cholesterol is required for chromatin remodeling and the direction of transcription programs controlling cell differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Mehrdad Khajavi, Yi Zhou, Alex J. Schiffer, Lauren Bazinet, Amy E. Birsner, Leonard Zon, Robert J. D'Amato
Summary: Through genetic studies in inbred mouse strains, the gene BASP1 was identified as significantly associated with angiogenesis. Further research has shown that BASP1 promotes angiogenesis by upregulating the beta-catenin gene and the Dll4/Notch1 signaling pathway, providing potential therapeutic avenues for angiogenesis-dependent diseases.
Article
Oncology
Anuj Anuj, Nina Reuven, Stefan G. E. Roberts, Ari Elson
Summary: The cytokine RANKL is involved in the differentiation of monocytes/macrophages into bone-resorbing osteoclasts, and it down-regulates the expression of BASP1 which is a negative regulator of osteoclastogenesis. Knocking down or eliminating BASP1 enhances RANKL-induced osteoclastogenesis, promoting cell-cell fusion and increasing mineral degrading abilities of osteoclasts.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Oncology
Nicolas Duployez, Loic Vasseur, Rathana Kim, Laetitia Largeaud, Marie Passet, Anais L'Haridon, Pierre Lemaire, Laurene Fenwarth, Sandrine Geffroy, Nathalie Helevaut, Karine Celli-Lebras, Lionel Ades, Delphine Lebon, Celine Berthon, Alice Marceau-Renaut, Meyling Cheok, Juliette Lambert, Christian Recher, Emmanuel Raffoux, Jean-Baptiste Micol, Arnaud Pigneux, Claude Gardin, Eric Delabesse, Jean Soulier, Mathilde Hunault, Herve Dombret, Raphael Itzykson, Emmanuelle Clappier, Claude Preudhomme
Summary: Tandem duplications (TDs) of the UBTF gene are found in both pediatric and adult acute myeloid leukemia (AML), with a frequency of 3% in adult patients. Adult patients with UBTF-TD AML have distinct characteristics, such as young age, low bone marrow blast infiltration, and high rates of WT1 mutations, FLT3-ITDs, and trisomy 8. These patients also have lower complete remission rates and similar survival outcomes compared to ELN 2022 intermediate/adverse risk patients. The study highlights the importance of recognizing UBTF-TD AML as a distinct entity in adults.
Article
Chemistry, Medicinal
Redouane Tabti, Francois Lamoureux, Celine Charrier, Benjamin Ory, Dominique Heymann, Embarek Bentouhami, Laurent Desaubry
Summary: Current therapeutic approaches for osteoporosis have limitations and potential adverse effects, but some sulfonylamidines have been shown to inhibit the formation of osteoclasts, suggesting potential for the development of new drugs. Compound IN45 demonstrated significant inhibition at a low concentration, indicating promise for anti-osteoporotic drug development.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Hematology
Huan Song, Yabin Liu, Yun Tan, Yi Zhang, Wen Jin, Li Chen, Shishuang Wu, Jinsong Yan, Junmin Li, Zhu Chen, Saijuan Chen, Kankan Wang
Summary: This study reveals the important role of noncoding variants in the leukemogenesis of acute promyelocytic leukemia (APL). Mutations in active enhancers, particularly in the WT1 gene, are associated with decreased expression of WT1 and recurrent biallelic inactivation, suggesting the tumor suppressor activity of WT1 in APL.
Article
Multidisciplinary Sciences
Alexander J. Moorhouse, Amy E. Loats, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The transcriptional corepressor BASP1 requires N-terminal myristoylation for its activity and functions through interactions with nuclear lipids. Removal of active histone modifications H3K9ac and H3K4me3 by BASP1 requires N-terminal myristoylation, while placement of the repressive histone modification H3K27me3 does not require lipidation. BASP1 regulates activity of multiple transcription factors and induces extensive changes in chromatin accessibility, with about 50% of BASP1 target genes showing lipidation-dependent chromatin compaction and transcriptional repression.
Article
Biochemistry & Molecular Biology
Zhongyou Que, Zhiwei Zhou, Sheng Liu, Wenhua Zheng, Bingxi Lei
Summary: The mechanism of dihydroartemisinin(DHA) inhibiting glioma migration and invasion in a ROS-DSB-dependent manner has been revealed. Extrachromosomal DNAs (ecDNAs) have potential in glioma treatment. EcDNA-BASP1 expression is found in glioma tissues and is positively correlated with patient prognosis. Knockdown of ecDNA-BASP1 promotes glioma proliferation, migration, and invasion. DHA treatment increases ecDNA-BASP1 content in nude mouse transplant tumors, exerting a better suppressive effect on glioma.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Louis N. Manganas, Irene Dura, Sivan Osenberg, Faith Semerci, Mehmet Tosun, Rachana Mishra, Luke Parkitny, Juan M. Encinas, Mirjana Maletic-Savatic
Summary: This study identified the epitope for the NSC-6 antibody as the signaling protein BASP1, which plays a key role in neurite outgrowth and plasticity. Western blot analysis revealed multiple BASP1 isoforms with varying expression levels, correlating with distinct developmental stages in mouse and human brains. The NSC-6 antibody could be a useful marker for neural stem cells in various developmental stages.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hao Liu, Hualin Fan, Pengcheng He, Haixia Zhuang, Xiao Liu, Meiting Chen, Wenwei Zhong, Yi Zhang, Cien Zhen, Yanling Li, Huilin Jiang, Tian Meng, Yiming Xu, Guojun Zhao, Du Feng
Summary: This study reveals the critical role of the inner mitochondrial membrane protein PHB1 in regulating permeability and integrity of mitochondria. Loss of PHB1 leads to alterations in mitochondrial function and increased release of mtDNA, resulting in enhanced inflammatory responses. Inflammatory stresses downregulate PHB1 expression, leading to increased mtDNA release and inflammation.
Article
Endocrinology & Metabolism
Karin M. Kirschner, Anna Foryst-Ludwig, Sabrina Gohlke, Chen Li, Roberto E. Flores, Ulrich Kintscher, Michael Schupp, Tim J. Schulz, Holger Scholz
Summary: The study shows that WT1 has a role as a white adipocyte determination factor in intra-abdominal WAT by suppressing thermogenic genes. Reducing the expression of WT1 in these fat depots may represent a novel treatment strategy for metabolic diseases.
Article
Oncology
Jiahui Zhang, Yanan Yin, Jiahui Wang, Jingjing Zhang, Hua Liu, Weiwei Feng, Wen Yang, Bruce Zetter, Yingjie Xu
Summary: This study investigates the characteristics of tumor cell proliferation, the role of the PI3K-Akt-mTOR axis, and the interaction between FKBP8 and PHB1, suggesting that PHB1 may act as a potential regulator of FKBP8.
FRONTIERS OF MEDICINE
(2021)
Article
Oncology
Alifiani Bonita Hartono, Hong-Jun Kang, Lawrence Shi, Whitney Phipps, Nathan Ungerleider, Alexandra Giardina, WeiPing Chen, Lee Spraggon, Romel Somwar, Krzysztof Moroz, David H. Drewry, Matthew E. Burow, Erik Flemington, Marc Ladanyi, Sean Bong Lee
Summary: Desmoplastic Small Round Cell Tumor (DSRCT) is a rare and aggressive malignant cancer caused by a chromosomal translocation. The study identified SIK1 as a direct target of the oncogenic transcription factor EWSR1-WT1, and depletion of SIK1 inhibited tumor cell growth and DNA replication. Combined inhibition of SIK1 and CHEK1 showed enhanced cytotoxicity in DSRCT cells.
Article
Biology
Min Chen, Fangfang Dong, Min Chen, Zhiming Shen, Haowei Wu, Changhuo Cen, Xiuhong Cui, Shilai Bao, Fei Gao
Summary: PRMT5 plays a crucial role in follicle development by regulating WT1 expression and facilitating the translation of Wt1 mRNA through methylation of HnRNPA1. It is essential for granulosa cell lineage maintenance, follicle structure, and steroidogenesis-related gene expression.
Article
Biology
Yaqin Yan, Jintian Tang, Qinfeng Yuan, Caiyun Liu, Xiaolin Chen, Hao Liu, Junbin Huang, Chonglai Bao, Tom Hsiang, Lu Zheng
Summary: Prohibitins play important roles in fungal virulence by regulating mitophagy, affecting the pathogenicity of Colletotrichum higginsianum.
COMMUNICATIONS BIOLOGY
(2022)
Correction
Oncology
N. Vasan, E. Toska, M. Scaltriti
ANNALS OF ONCOLOGY
(2020)
Article
Genetics & Heredity
Guotai Xu, Sagar Chhangawala, Emiliano Cocco, Pedram Razavi, Yanyan Cai, Jordan E. Otto, Lorenzo Ferrando, Pier Selenica, Erik Ladewig, Carmen Chan, Arnaud Da Cruz Paula, Matthew Witkin, Yuanming Cheng, Jane Park, Cristian Serna-Tamayo, HuiYong Zhao, Fan Wu, Mirna Sallaku, Xuan Qu, Alison Zhao, Clayton K. Collings, Andrew R. D'Avino, Komal Jhaveri, Richard Koche, Ross L. Levine, Jorge S. Reis-Filho, Cigall Kadoch, Maurizio Scaltriti, Christina S. Leslie, Jose Baselga, Eneda Toska
Article
Endocrinology & Metabolism
Zachary C. Ahart, Laura E. Martin, Bailey R. Kemp, Debarghya Dutta Banik, Stefan G. E. Roberts, Ann-Marie Torregrossa, Kathryn F. Medler
Article
Oncology
Patrizia Mondello, Saber Tadros, Matt Teater, Lorena Fontan, Aaron Y. Chang, Neeraj Jain, Haopeng Yang, Shailbala Singh, Hsia-Yuan Ying, Chi-Shuen Chu, Man Chun John Ma, Eneda Toska, Stefan Alig, Matthew Durant, Elisa de Stanchina, Sreejoyee Ghosh, Anja Mottok, Loretta Nastoupil, Sattva S. Neelapu, Oliver Weigert, Giorgio Inghirami, Jose Baselga, Anas Younes, Cassian Yee, Ahmet Dogan, David A. Scheinberg, Robert G. Roeder, Ari M. Melnick, Michael R. Green
Article
Genetics & Heredity
Debarghya Dutta Banik, Eric D. Benfey, Laura E. Martin, Kristen E. Kay, Gregory C. Loney, Amy R. Nelson, Zachary C. Ahart, Barrett T. Kemp, Bailey R. Kemp, Ann-Marie Torregrossa, Kathryn F. Medler
Article
Oncology
Amaia Arruabarrena-Aristorena, Jesper L. V. Maag, Srushti Kittane, Yanyan Cai, Wouter R. Karthaus, Erik Ladewig, Jane Park, Srinivasaraghavan Kannan, Lorenzo Ferrando, Emiliano Cocco, Sik Y. Ho, Daisylyn S. Tan, Mirna Sallaku, Fan Wu, Barbara Acevedo, Pier Selenica, Dara S. Ross, Matthew Witkin, Charles L. Sawyers, Jorge S. Reis-Filho, Chandra S. Verma, Ralf Jauch, Richard Koche, Jose Baselga, Pedram Razavi, Eneda Toska, Maurizio Scaltriti
Article
Biochemistry & Molecular Biology
Tareg Belali, Chigeru Wodi, Bethany Clark, Man-Kim Cheung, Tim J. Craig, Gabrielle Wheway, Nicole Wagner, Kay-Dietrich Wagner, Stefan Roberts, Sean Porazinski, Michael Ladomery
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2020)
Article
Multidisciplinary Sciences
Amy E. Loats, Samantha Carrera, Anna F. Fleming, Abigail R. E. Roberts, Alice Sherrard, Eneda Toska, Alexander J. Moorhouse, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The study reveals that the lipidated protein BASP1 recruits cholesterol to the promoter region of target genes in the cell nucleus through a conserved interaction motif, and that BASP1's interaction with cholesterol is required for chromatin remodeling and the direction of transcription programs controlling cell differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Andrew L. Wolfe, Qingwen Zhou, Eneda Toska, Jacqueline Galeas, Angel A. Ku, Richard P. Koche, Sourav Bandyopadhyay, Maurizio Scaltriti, Carlito B. Lebrilla, Frank McCormick, Sung Eun Kim
Summary: The enzyme UGP2, which synthesizes UDP-glucose, plays a crucial role in maintaining pancreatic ductal adenocarcinoma (PDAC) growth. Its transcription is directly regulated by the YAP-TEAD complex, and loss of UGP2 leads to decreased glycogen levels and defects in glycosylation of important targets for PDAC survival.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Letter
Hematology
Megan Wagstaff, Olga Tsaponina, Gilian Caalim, Hayley Greenfield, Leanne Milton-Harris, Erika J. Mancini, Allison Blair, Kate J. Heesom, Alex Tonks, Richard L. Darley, Stefan G. Roberts, Rhys G. Morgan
Article
Multidisciplinary Sciences
Alexander J. Moorhouse, Amy E. Loats, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The transcriptional corepressor BASP1 requires N-terminal myristoylation for its activity and functions through interactions with nuclear lipids. Removal of active histone modifications H3K9ac and H3K4me3 by BASP1 requires N-terminal myristoylation, while placement of the repressive histone modification H3K27me3 does not require lipidation. BASP1 regulates activity of multiple transcription factors and induces extensive changes in chromatin accessibility, with about 50% of BASP1 target genes showing lipidation-dependent chromatin compaction and transcriptional repression.
Editorial Material
Oncology
Ryan Blawski, Eneda Toska
Summary: In this study, the authors investigated the mechanisms of differential therapy response in invasive lobular carcinomas (ILC) compared to invasive ductal carcinomas (IDC). They found an alternative chromatin accessibility state in ILC driven by the pioneer factor FOXA1, which promotes the transcription of genes associated with tumor progression and tamoxifen resistance. Targeting the FOXA1 enhancer region blocks this transcriptional program and inhibits ILC proliferation.
Article
Oncology
Anuj Anuj, Nina Reuven, Stefan G. E. Roberts, Ari Elson
Summary: The cytokine RANKL is involved in the differentiation of monocytes/macrophages into bone-resorbing osteoclasts, and it down-regulates the expression of BASP1 which is a negative regulator of osteoclastogenesis. Knocking down or eliminating BASP1 enhances RANKL-induced osteoclastogenesis, promoting cell-cell fusion and increasing mineral degrading abilities of osteoclasts.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Physiology
Debarghya Dutta Banik, Kathryn F. Medler
Summary: The taste system uses distinct signaling pathways to detect chemicals in potential food items, with bitter, sweet and umami stimuli activating GPCR pathways while salt and sour are detected by ionotropic receptors. Recent studies suggest that the signaling processes in taste cells are more complex than previously appreciated.
CURRENT OPINION IN PHYSIOLOGY
(2021)