Article
Cell Biology
Ziwen Lu, Sirui Yuan, Lingling Ruan, Zhigang Tu, Hanqing Liu
Summary: Partitioning-defective protein 6 (Par6) family proteins play a critical role in the occurrence and development of ovarian cancer. This study revealed that PARD6A is highly expressed in advanced stages of ovarian cancer and is associated with poor differentiation and lymphatic metastases. Knockdown of PARD6A suppressed EMT and metastasis in ovarian cancer cells, while overexpression of PARD6A promoted EMT. PARD6A affected EMT through the SNAIL1 signaling pathway and modulated the expression of VIMENTIN and E-cadherin. ILK was identified as a regulator of SNAIL1 expression. This study highlights the importance of PARD6A in ovarian cancer metastasis and provides a potential therapeutic target for drug development.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
So Dam Lee, Haengdueng Jeong, Bo Ram Hwang, Byeong Min Yu, Yejin Cho, Ki Teak Nam, Hyunki Kim, Yong Chan Lee
Summary: Strains of Helicobacter pylori positive for CagA may contribute to the development of gastric cancer through the induction of CK2 activation and CK28 degradation. The downregulation of CK28 and subsequent activation of AKT and Snail signaling pathways may play a crucial role in the epithelial-to-mesenchymal transition (EMT) in gastric cancer. CK2 could be a potential molecular target for the treatment of H. pylori-infected gastric cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Liubov A. Tashireva, Olga E. Savelieva, Evgeniya S. Grigoryeva, Yuri V. Nikitin, Evgeny V. Denisov, Sergey V. Vtorushin, Marina V. Zavyalova, Nadezhda V. Cherdyntseva, Vladimir M. Perelmuter
Summary: Significant heterogeneity of CTCs in breast cancer is observed, particularly in terms of EMT plasticity. The majority of CTC phenotypes exhibit epithelial-mesenchymal plasticity, contributing to the understanding of the metastatic process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Xu Xu, Yunan Hou, Niya Long, Lishi Jiang, Zhangwei Yan, Yuan Xu, Ying Lv, Xin Xiang, Hua Yang, Jian Liu, Xiaolan Qi, Liangzhao Chu
Summary: This study found that the expression of TPPP3 is higher in glioma compared to normal brain tissue, and increases with the grade of glioma. Up-regulation of TPPP3 enhances migration, invasion, and proliferation abilities, while reducing apoptosis in glioblastoma cells. TPPP3 also affects the process of EMT by regulating the expression of Snail1 protein. Low TPPP3 expression is associated with better survival expectations in glioblastoma patients. This study provides new insights and directions for further research on the role of TPPP3 in glioblastoma.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Eloise M. Grasset, Matthew Dunworth, Gaurav Sharma, Melanie Loth, Joseph Tandurella, Ashley Cimino-Mathews, Melissa Gentz, Sydney Bracht, Meagan Haynes, Elana J. Fertig, Andrew J. Ewald
Summary: Triple-negative breast cancer (TNBC) is an aggressive subtype associated with early metastatic recurrence and worse patient outcomes. Our study demonstrates the important and complex role of epithelial-mesenchymal transition (EMT) programs during TNBC metastasis. We observed the presence of a large population of hybrid epithelial-mesenchymal cells in TNBC tumors, which express both epithelial and mesenchymal characteristics and promote invasion in vitro. The mesenchymal marker vimentin promotes invasion and represses metastatic outgrowth. We also identified distinct patterns of EMT transcription factor utilization during invasion and colony formation. Our findings suggest a sequential activation of multiple EMT molecular programs during the metastatic cascade. Longitudinal single-cell RNA analysis detected three different EMT-related molecular patterns, indicating a complex spectrum of epithelial, hybrid E/M, and mesenchymal cell states within metastases. Our results highlight the heterogeneity and multiple molecular strategies for successful distant organ colonization in TNBC.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mireia Pujals, Carla Mayans, Chiara Bellio, Olga Mendez, Emanuela Greco, Roberta Fasani, Merce Alemany-Chavarria, Esther Zamora, Laura Padilla, Francesc Mitjans, Paolo Nuciforo, Francesc Canals, Lara Nonell, Maria Abad, Cristina Saura, Josep Tabernero, Josep Villanueva
Summary: Epithelial/mesenchymal (E/M) plasticity is important in both embryogenesis and tumorigenesis. The role of receptor for advanced glycation end products (RAGE) in triple-negative breast cancer (TNBC) is unclear. This study shows that RAGE signaling maintains the mesenchymal phenotype of aggressive TNBC cells through the expression of SNAIL1 and its crosstalk with the TGF-β pathway. RAGE inhibition promotes epithelial features and reduces migration and invasion capacities. In vivo inhibition of RAGE reduces metastasis incidence and improves survival.
Editorial Material
Pharmacology & Pharmacy
Marco P. Licciardello, Paul Workman
Summary: Casein kinase 2, a potential therapeutic target in cancer due to its high expression, did not exhibit broad antiproliferative activity in cancer cells when targeted by the new inhibitor SGC-CK2-1, developed by Wells and colleagues.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Li-Fen Lin, Yi-Ting Li, Hui Han, Shun-Guo Lin
Summary: Our study investigated the biological role of miR-205 in breast cancer, demonstrating that its overexpression could inhibit cell proliferation and chemoresistance by targeting the HOXD9-Snail1 axis. This research provides novel insight for therapeutic strategies of breast cancer through targeting miR-205/HOXD9/Snail1.
Article
Oncology
Rongkun Li, Lili Zhu, Yangxizi Peng, Xiaoxin Zhang, Chunhua Dai, Dejun Liu
Summary: TRIM50 is identified as a tumor suppressor in pancreatic cancer, which inhibits cancer progression and reverses EMT by degrading Snail1.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Guidantonio Malagoli Tagliazucchi, Anna J. Wiecek, Eloise Withnell, Maria Secrier
Summary: The authors develop a method to evaluate EMT transformation and trajectories in cancer transcriptomics data, characterising EMT macro-states, including a hybrid state, and EMT hallmarks. The study explores the spatial heterogeneity of EMT transformation and its interaction patterns with cytotoxic, NK cells and fibroblasts in the tumor microenvironment. It also provides a catalogue of genomic events underlying distinct evolutionary constraints on EMT transformation.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Huanhuan Deng, Leihong Deng, Haichao Chao, Zhaojun Yu, Jianbiao Huang, Zhen Song, Lifen Peng, Tao Zeng
Summary: Bladder cancer (BLCA) is a common cancer with high mortality ranking 9th. The relationship between autophagy and epithelial to mesenchymal transition (EMT) in BLCA is poorly understood. This study identified the autophagy-related gene RAB14 and demonstrated its promotion of EMT, migration, and invasion in bladder cancer cells through the Akt-associated autophagic pathway. Knocking down RAB14 inhibited EMT and autophagy in T24 cells, and RAB14 levels were positively correlated with key autophagy genes LC3B and Beclin1. These findings suggest the potential role of RAB14 in BLCA progression.
CELL DEATH DISCOVERY
(2023)
Article
Environmental Sciences
Rong Li, Runze Shang, Shunle Li, Yifan Ren, Lin Shen, Longbao Yang, Shuo Chen, Xi Chen, Junhui Li, Meng Xu
Summary: The study found that high expression of LOXL3 in HCC was significantly correlated with tumor size and clinical stage, predicting a poor prognosis. Experimental results demonstrated that LOXL3 promoted HCC EMT process through the Snail1/USP4-mediated circulation loop and Wnt/β-catenin signaling pathway.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Oncology
Namhee Kim, Chae Young Hwang, Taeyoung Kim, Hyunjin Kim, Kwang-Hyun Cho
Summary: The process of epithelial-to-mesenchymal transition (EMT) in cancer cells, which contributes to metastasis and drug resistance, can be reversed through a systems biology approach. By targeting a core-regulatory circuit involving SMAD4 and ERK signaling inhibition, along with p53 activation, complete EMT reversal (rEMT) can be achieved without hybrid EMT properties. This reprogramming strategy restores chemosensitivity, offering potential therapeutic targets to eradicate metastatic potential by subverting EMT while avoiding hybrid states.
Article
Oncology
Estefania Carrasco-Garcia, Lidia Lopez, Veronica Moncho-Amor, Fernando Carazo, Paula Aldaz, Manuel Collado, Donald Bell, Ayman Gaafar, Eva Karamitopoulou, Alexandar Tzankov, Manuel Hidalgo, Angel Rubio, Manuel Serrano, Charles H. Lawrie, Robin Lovell-Badge, Ander Matheu
Summary: The study shows that SOX9 plays a crucial role in the progression and metastasis of pancreatic cancer by regulating stemness and EMT processes. High levels of SOX9 are associated with metastases in clinical samples.
Article
Biochemistry & Molecular Biology
Ji-Yun Yoon, Seung-Un Seo, Seon-Min Woo, Taeg-Kyu Kwon
Summary: Ubiquitination is a post-translational modification that leads to protein degradation. Deubiquitinases, like USP41, remove ubiquitin chains and stabilize substrates. USP41 was found to be overexpressed in breast cancer cells and its knockdown inhibited cell migration and growth. It was also observed that USP41 interacts with and inhibits ubiquitination of Snail, leading to its stabilization and increased migration of breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Astronomy & Astrophysics
Daniel Maschmann, Anaelle Halle, Anne-Laure Melchior, Francoise Combes, Igor V. Chilingarian
Summary: In this paper, synthetic SDSS spectroscopic observations and simulations are used to explore the origin of emission lines with a double-peak shape in galaxies. It is found that the double-peak signature is connected to the central part of the rotation curve and can be created by bars and minor mergers. Major mergers, on the other hand, do not match the observations. Understanding the origin of double-peaks can provide important tools for studying galaxy mass growth in future high redshift surveys.
ASTRONOMY & ASTROPHYSICS
(2023)
Article
Multidisciplinary Sciences
Sebastien Durand, Marion Bruelle, Fleur Bourdelais, Bigitha Bennychen, Juliana Blin-Gonthier, Caroline Isaac, Aurelia Huyghe, Sylvie Martel, Antoine Seyve, Christophe Vanbelle, Annie Adrait, Yohann Coute, David Meyronet, Frederic Catez, Jean-Jacques Diaz, Fabrice Lavial, Emiliano P. Ricci, Francois Ducray, Mathieu Gabut
Summary: The study reveals that RSL24D1 is highly expressed in both mouse and human pluripotent stem cells and is involved in the biogenesis of 60S subunits. Depletion of RSL24D1 impairs global translation, particularly of pluripotency factors and components from the Polycomb Repressive Complex 2 (PRC2), leading to altered self-renewal and lineage commitment choices in embryonic stem cells.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
David da Silva Barreira, Julie Laurent, Jessica Lourenco, Julia Novion Ducassou, Yohann Coute, Jean Guzzo, Aurelie Rieu
Summary: Biofilms are a major concern in the food industry and healthcare. Probiotic membrane vesicles (MVs) have shown strong antibiofilm activity against Salmonella enterica serovar Enteritidis (S. Enteritidis) without affecting bacterial growth. The antibiofilm properties of the MVs were found to be associated with specific proteins, particularly peptidoglycan hydrolases (PGHs).
SCIENTIFIC REPORTS
(2023)
Editorial Material
Clinical Neurology
Juliette Svahn, Laurent Coudert, Nathalie Streichenberger, Alexandra Kraut, Alice Gravier-Dumonceau-Mazelier, Ludivine Rotard, Laurence Calemard-Michel, Rita Menassa, Elisabeth Errazuriz-Cerda, Lara Chalabreysse, Alexis Osseni, Christophe Vial, Laurentiu Jomir, Francois Tronc, Do Le Duy, Emilien Bernard, Vincent Gache, Yohann Coute, Vincent Jacquemond, Laurent Schaeffer, Pascal Leblanc
Summary: This study reports a case of acquired autoimmune rippling muscle disease (iRMD) associated with thymoma and AchR antibody negativity. The patient was found to have circulating MURC/Cavin-4 autoantibodies, and the histological features of the patient's muscles were similar to those commonly reported in caveolinopathies. After tumor resection and immunotherapy, the levels of autoantibodies significantly decreased, the rippling phenotype disappeared completely, and the patient achieved full remission. Therefore, MURC/Cavin-4 autoantibodies may play a pathogenic role in the development of paraneoplastic iRMD associated with thymoma.
NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION
(2023)
Article
Biology
Melanie Bodnar-Wachte, Anne-Laure Huber, Julie Gorry, Sabine Hacot, Delphine Burlet, Laetitia Gerossier, Baptiste Guey, Nadege Goutagny, Birke Bartosch, Elise Ballot, Julie Lecuelle, Caroline Truntzer, Francois Ghiringhelli, Benedicte F. Py, Yohann Coute, Annabelle Ballesta, Sylvie Lantuejoul, Janet Hall, Agnes Tissier, Virginie Petrilli
Summary: NLRP3 interacts with ATM kinase to control the activation of the DNA damage response, independent of its inflammasome activity. Down-regulated expression of NLRP3 in lung and breast cancers is associated with better patient overall survival. This discovery reveals a novel non-immune function for NLRP3 in maintaining genome integrity.
LIFE SCIENCE ALLIANCE
(2023)
Article
Astronomy & Astrophysics
L. K. Hunt, F. Belfiore, F. Lelli, B. T. Draine, A. Marasco, S. Garcia-Burillo, G. Venturi, F. Combes, A. Weiss, C. Henkel, K. M. Menten, F. Annibali, V. Casasola, M. Cignoni, A. McLeod, M. Tosi, M. Beltran, A. Concas, G. Cresci, M. Ginolfi, N. Kumari, F. Mannucci
Summary: In this paper, a dust-based method and observational data were used to quantify the relationship between CO emission and molecular hydrogen column density in three nearby metal-poor starburst galaxies. The results showed that the relationship between CO emission and molecular hydrogen column density is diverse, depending not only on metallicity but also on the CO brightness temperature and beam size.
ASTRONOMY & ASTROPHYSICS
(2023)
Article
Astronomy & Astrophysics
F. Combes, N. Gupta, S. Muller, S. Balashev, P. P. Deka, K. L. Emig, H. -R. Klockner, D. Klutse, K. Knowles, A. Mohapatra, E. Momjian, P. Noterdaeme, P. Petitjean, P. Salas, R. Srianand, J. D. Wagenveld
Summary: The BL Lac object PKS 1413+135 was observed by MALS, detecting H I and OH absorption lines at z = 0.24671. The H I absorption line has a high signal-to-noise ratio, a narrow central component, and a red wing, while the OH 1720 MHz line is clearly detected in emission.
ASTRONOMY & ASTROPHYSICS
(2023)
Article
Astronomy & Astrophysics
S. Muller, I. Marti-Vidal, F. Combes, M. Gerin, A. Beelen, C. Horellou, M. Guelin, S. Aalto, J. H. Black, E. van Kampen
Summary: This article studies the time variations of the PKS 1830-211 system to obtain information about both the activity of the quasar and small-scale structures in the absorber. The results show that the system's variability is related to the quasar's intrinsic activity, and significant variations are observed in the absorption profile of the foreground absorber.
ASTRONOMY & ASTROPHYSICS
(2023)
Article
Astronomy & Astrophysics
Gerrit Schellenberger, Ewan O'Sullivan, Simona Giacintucci, Jan Vrtilek, Laurence P. David, Francoise Combes, Laura Birzan, Hsi-An Pan, Lihwai Lin
Summary: The galaxy group NGC 6338 is undergoing a violent merger with rush of central dominant galaxies, leading to gas heating and shock fronts. However, the gas in the central galaxies remains cool and there are indications of feedback from active galactic nuclei. Radio data shows the presence of large, old lobes in the southern central galaxy, coinciding with X-ray cavities, and a smaller, younger set of lobes perpendicular to the larger lobes. Spectral analysis confirms two feedback cycles. This merger presents a prominent case where the direction of jet-mode feedback between two cycles changed dramatically.
ASTROPHYSICAL JOURNAL
(2023)
Article
Astronomy & Astrophysics
Kimberly L. L. Emig, Neeraj Gupta, Pedro Salas, Sebastien Mueller, Sergei A. A. Balashev, Francoise Combes, Emmanuel Momjian, Yiqing Song, Preshanth Jagannathan, Partha P. P. Deka, Gyula I. G. Joezsa, Hans-Rainer Kloeckner, Abhisek Mohapatra, Pasquier Noterdaeme, Patrick Petitjean, Raghunathan Srianand, Jonah D. D. Wagenveld
Summary: We detected stimulated hydrogen radio recombination line (RRL) emission from ionized gas in a z = 0.89 galaxy. This is the second detection of RRLs outside of the local Universe and the first associated with hydrogen. The RRL emission contains two velocity components and is coincident with H I 21 cm and OH 18 cm absorption.
ASTROPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Karen Coste, Shaam Bruet, Caroline Chollat-Namy, Odile Filhol, Claude Cochet, Denis Gallot, Geoffroy Marceau, Loic Blanchon, Vincent Sapin, Corinne Belville
Summary: Fetal membranes (FM) play a crucial role at the feto-maternal interface. Rupture of FM at term involves sterile inflammation mechanisms, including the activation of the RAGE pathway. The protein kinase CK2 is also implicated in inflammation, and this study aims to characterize the expressions of RAGE and CK2 and their potential regulatory relationship.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Astronomy & Astrophysics
Mahmoud Hashim, Amr A. El-Zant, Jonathan Freundlich, Justin Read, Francoise Combes
Summary: The cold dark matter (CDM) structure formation faces challenges on small scales, particularly the 'cusp-core' problem. Interactions with baryons, such as feedback or orbiting clumps of gas or stars, can drive CDM out of Galactic Centres. Core formation in a hydrodynamic simulation is interpreted using a theoretical formulation, which characterizes the effects of complex hydrodynamics and 'subgrid physics'. The power spectrum of gaseous fluctuations follows a power law over a range of scales, suitable for a fully turbulent compressible medium.
MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY
(2023)
Review
Biochemical Research Methods
Subina Mehta, Matthias Bernt, Matthew Chambers, Matthias Fahrner, Melanie Christine Foell, Bjoern Gruening, Carlos Horro, James E. Johnson, Valentin Loux, Andrew T. Rajczewski, Oliver Schilling, Yves Vandenbrouck, Ove Johan Ragnar Gustafsson, W. C. Mike Thang, Cameron Hyde, Gareth Price, Pratik D. Jagtap, Timothy J. Griffin
Summary: The Galaxy ecosystem offers a range of open-source tools for MS-based proteomics analyses, providing an adaptable, scalable, and accessible computing environment. This community-supported resource is crucial for basic biological and clinical studies, and ongoing developments are underway to meet emerging challenges in MS-based proteomic informatics.
EXPERT REVIEW OF PROTEOMICS
(2023)
Article
Virology
Federico Marziali, Yuxin Song, Xuan-Nhi Nguyen, Lucid Belmudes, Julien Burlaud-Gaillard, Philippe Roingeard, Yohann Coute, Andrea Cimarelli
Summary: This study identified NDFIP2 protein adaptor as an important regulator of IFITM3 levels and showed that NDFIP2 recruits IFITM3 and NEDD4 and mediates their distribution in lysosomal vesicles, thereby regulating the accumulation of IFITM3. The study reveals a novel and potentially dynamic layer of regulation of IFITM3.
Article
Multidisciplinary Sciences
Domenico Iuso, Isabel Garcia-Saez, Yohann Coute, Yoshiki Yamaryo-Botte, Elisabetta Boeri Erba, Annie Adrait, Nour Zeaiter, Malgorzata Tokarska-Schlattner, Zuzana Macek Jilkova, Faycal Boussouar, Sophie Barral, Luca Signor, Karine Couturier, Azadeh Hajmirza, Florent Chuffart, Ekaterina Bourova-Flin, Anne-Laure Vitte, Lisa Bargier, Denis Puthier, Thomas Decaens, Sophie Rousseaux, Cyrille Botte, Uwe Schlattner, Carlo Petosa, Saadi Khochbin
Summary: Nucleoside diphosphate kinases (NME1/2) regulate nucleotide homeostasis and bind to CoA. NME2 mediates gene transcription response in liver cells under high-fat diet, repressing fatty acid accumulation and activating protective gene expression program.
