Article
Biochemistry & Molecular Biology
Kun Zhang, Yan Liang, Wancong Zhang, Ning Zeng, Shijie Tang, Ruoxi Tian
Summary: This study identified that upregulated expression of KRT81 in melanoma tissues and cell lines. Knockdown of KRT81 inhibited proliferation, colony formation, migration, invasion, and promoted apoptosis of melanoma cells, as well as increased chemosensitivity. Mechanistically, KRT81 knockdown downregulated IL-8, suggesting KRT81 as a potential therapeutic target for melanoma.
DNA AND CELL BIOLOGY
(2021)
Article
Oncology
Xinxin Zhou, Jiayuan He, Qingyuan Wang, Teng Ma
Summary: The study revealed that miR-128-3p acts as a tumor suppressor in malignant melanoma cells by inhibiting proliferation, migration, invasion, and inducing apoptosis. The oncogene neurotrophin receptor 3 (NTRK3) was found to be up-regulated in MM cells, enhancing their malignant behaviors. The interaction between miR-128-3p and NTRK3 genes modulate the malignant behavior of MM, suggesting potential new therapeutic targets.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Mathilde Kerherve, Sara Rosinska, Kilian Trillet, Alya Zeinaty, Magalie Feyeux, Steven Nedellec, Julie Gavard
Summary: Glioblastoma multiforme (GBM) is a rare but devastating brain tumor in adults, with current treatments being generally ineffective. This study focused on a population of self-sustaining transformed cells with stem-like properties (GSCs) within GBM, which play a crucial role in tumor initiation, growth, invasion, and recurrence. An ex vivo model was established to explore the invasive behavior of GSCs, and it was found that the glycoprotein Neuropilin-1 (NRP1) contributes to their spreading and invasion. This study proposes a reliable approach to studying invasive properties of patient glioma cells and identifies NRP1 as a mediator in this malignant process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Yang Yang, Yizhe Wang, Xiaofang Che, Kezuo Hou, Jie Wu, Chunlei Zheng, Yang Cheng, Yunpeng Liu, Xuejun Hu, Jingdong Zhang
Summary: The study revealed that the integrin alpha 5/FAK/STAT3/AKT signaling pathway promotes icotinib resistance and malignancy in NSCLC cells. Targeting this pathway may provide a promising strategy to overcome drug resistance.
Article
Biochemistry & Molecular Biology
Anpei Huang, Ruizhi Wang, Ji Cui, Ying Gao, Zheng Yin, Lianzhou Chen, Meifang He, Wen Li
Summary: This study found that GOLPH3 is overexpressed in colorectal cancer tissues and cells, and it promotes the metastasis and progression of colorectal cancer by facilitating STAT3 activation and regulating the expression of EMT transcription factor ZEB1 and integrin alpha 3.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna Maria Luciano, Ana B. Perez-Oliva, Victoriano Mulero, Donatella Del Bufalo
Summary: Apoptosis is crucial for eliminating damaged cells in multicellular organisms. Bcl-xL, an anti-apoptotic protein, plays significant roles in cancer, including melanoma, by regulating cell survival, migration, invasion, and angiogenesis. Targeting Bcl-xL may offer potential benefits for cancer patients, but further studies are needed to develop safe and effective inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Lijun Zhong, Lin Tang, Xiaoxia He
Summary: It has been discovered that ANGPTL3 plays an oncogenic role in several types of human malignancies. This study found that ANGPTL3 is highly expressed in cervical cancer cells and silencing ANGPTL3 can inhibit cell proliferation, migration, invasion, and angiogenesis. The inhibitory effect of ANGPTL3 can be offset by upregulating the expression of alpha v beta 3, which also promotes blood vessel formation and the secretion of VEGF and VEGFR2 in cervical cancer cells.
Article
Cell Biology
Justyna Mazurkiewicz, Aleksandra Simiczyjew, Ewelina Dratkiewicz, Magdalena Kot, Katarzyna Pietraszek-Gremplewicz, Dominika Wilk, Marcin Zietek, Rafal Matkowski, Dorota Nowak
Summary: This study provides a more in-depth understanding of the role of keratinocytes in the melanoma microenvironment, highlighting their unique secretome and mediators of invasive abilities, which could be utilized by cancer cells to support their invasion of surrounding tissues.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Medical Laboratory Technology
Yang Yu, Yang Gao
Summary: The study demonstrates that high expression of SSRP1 is associated with poor prognosis in malignant melanoma patients. Inhibiting SSRP1 can suppress melanoma cell proliferation, migration, and invasion by affecting the MAPK signaling pathway. These findings suggest that SSRP1 may be a potential target for clinical treatment of melanoma.
ANNALS OF CLINICAL AND LABORATORY SCIENCE
(2021)
Article
Dermatology
Li Ni, Pin Li, Mingming Li, Shuhong Huang, Ningning Dang
Summary: This study characterized the function and interaction of ITGAX, SERPINB8, and furin in BRAF V600E mutant melanoma. It was found that SERPINB8 and furin regulate the expression of ITGAX, which significantly promotes the proliferation and invasion of melanoma cells.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ewa Brzozowska, Sameer Deshmukh
Summary: Integrins are crucial for cell adhesion, migration, and positioning. Studies have shown that the expression levels of Integrin αVβ6 are closely related to malignant diseases and patient prognosis. It plays a significant role in cancer progression and the activation of transforming growth factor β(TGF-β). While essential for normal function in healthy individuals, it can also be a target for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Satoko Yamamoto, Kei Kaneko, Yuki Ohkawa, Pu Zhang, Kazunori Hamamura, Nai-Kong V. Cheung, Norihiro Kotani, Koichi Honke, Tetsuya Okajima, Mariko Kambe, Orie Tajima, Keiko Furukawa, Koichi Furukawa
Summary: GD2 and integrin beta 1 physically associate in the microdomain of the cell membrane, leading to enhanced malignant phenotypes of melanoma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Guixi Zheng, Hakim Bouamar, Matyas Cserhati, Carla R. Zeballos, Isha Mehta, Habil Zare, Larry Broome, Ruolei Hu, Zhao Lai, Yidong Chen, Francis E. Sharkey, Meenakshi Rani, Glenn A. Halff, Francisco G. Cigarroa, Lu-Zhe Sun
Summary: The study found that ITGA6 is upregulated in HCC tumors and has a malignant promoting role in HCC cells through integrin alpha 6 beta 4 complex. Thus, integrin alpha 6 beta 4 may be a therapeutic target for treating HCC patients.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Cell Biology
Yujing Gao, Yujie Fang, Yongli Huang, Rui Ma, Xixi Chen, Fang Wang, Xiuying Pei, Yuanqi Gao, Xuehua Chen, Xinrui Liu, Jingxuan Shan, Pu Li
Summary: MIIP has been identified as a favorable indicator of prognosis in triple-negative breast cancer. It exerts its tumor-suppressive effects by inhibiting angiogenesis, proliferation, and metastasis of cancer cells. MIIP interacts with ITGB3 and suppresses its downstream signaling, resulting in downregulated VEGFA production and epithelial mesenchymal transition. The RGD motif of MIIP is essential for its binding with ITGB3 and efficient tumor suppression.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Patrick Meylan, Christine Pich, Carine Winkler, Stefanie Ginster, Lionel Mury, Marie Sgandurra, Rene Dreos, Dennie Tompers Frederick, Marc Hammond, Genevieve Marie Boland, Liliane Michalik
Summary: TXNIP, regulated by PPARγ in melanoma cells, is associated with tumor regression, cell cycle arrest, and benign melanocytic lesions. Reduced expression of TXNIP is linked to advanced melanoma and disease progression.
SCIENTIFIC REPORTS
(2021)
Article
Physics, Applied
Christian Weisenstein, Merle Richter, Anna Katharina Wigger, Anja Bosserhoff, Peter Haring Bolivar
Summary: This article proposes an approach to use metamaterials for biomolecule detection in water by introducing substrate-integrated microfluidics. The approach allows highly sensitive detection in strongly absorbing liquids without affecting the metamaterial structure design.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Markus V. Heppt, Anja Wessely, Eva Hornig, Claudia Kammerbauer, Saskia A. Graf, Robert Besch, Lars E. French, Alexander Matthies, Silke Kuphal, Melanie Kappelmann-Fenzl, Anja K. Bosserhoff, Carola Berking
Summary: The neural crest transcription factor BRN3A is essential for melanoma cell proliferation and survival. The mechanism underlying its aberrant expression is unknown. This study revealed that the epigenetic regulator HDAC2 plays a key role in the expression of BRN3A in melanocytes and melanoma cells. Inhibition of HDAC2 increased BRN3A expression, highlighting the importance of epigenetic mechanisms in regulating melanoma oncogenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ines Boehme-Schaefer, Sandra Loerentz, Anja Katrin Bosserhoff
Summary: This study reveals that the amino acid transporter SNAT1 is overexpressed in malignant melanoma and plays a crucial role in the proliferation, migration, invasion, and senescence of melanoma cells. Therefore, SNAT1 may be a potential target for melanoma therapy.
Article
Cell Biology
Tom Zimmermann, Michaela Pommer, Viola Kluge, Chafia Chiheb, Susanne Muehlich, Anja-Katrin Bosserhoff
Summary: Detection and quantification of senescent cells is challenging. It is accepted to use a combination of markers and cellular characteristics to define senescent cells in vitro. This study provides a comprehensive comparison of biomarkers and cellular characteristics used to detect senescence in melanocytic systems, aiming to support the development of standardized senescence detection and quantification.
Article
Biochemistry & Molecular Biology
Lisa Linck-Paulus, Tina Meissgeier, Katharina Pieger, Anselm H. C. Horn, Alexander Matthies, Stefan Fischer, Gunter Meister, Heinrich Sticht, Melanie Kappelmann-Fenzl, Anja K. Bosserhoff
Summary: A novel splice variant of Argonaut 2, AGO2-ex1/3, is found to play an important role in melanoma cells. Expression of AGO2-ex1/3 provides a survival advantage for melanoma cells, while knockdown leads to reduced proliferation and increased apoptosis, affecting miRNA function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Nicole Rachinger, Nora Mittag, Ines Boehme-Schaefer, Wei Xiang, Silke Kuphal, Anja K. Bosserhoff
Summary: This study reveals a novel physiological role of alpha-Synuclein (aSyn) in melanocytic morphology and its unknown function in pigment secretion in melanocytes.
Article
Chemistry, Analytical
Christian Weisenstein, Merle Richter, Anna Katharina Wigger, Anja K. Bosserhoff, Peter Haring Bolivar
Summary: THz frequency range is considered suitable for developing new bio-analytical methods due to the presence of THz-excited vibrational modes in biomacromolecules. In this study, a scalable THz metamaterial-based biosensor was used to investigate DNA samples at multiple frequencies. The results showed that the resonance frequency shift of the metamaterial depended on the presence of DNA and varied with frequency. The experiments demonstrated a significant difference in the power function between dsDNA and ssDNA, indicating an advantage for dsDNA detection and increased sensitivity at higher frequencies.
Article
Oncology
Felix Seefried, Lucia Haller, Shinji Fukuda, Aranya Thongmao, Nadja Schneider, Jochen Utikal, Shigeki Higashiyama, Anja Katrin Bosserhoff, Silke Kuphal
Summary: AREG plays multiple roles in melanoma, acting as a ligand for EGFR and an intracellular signaling molecule. Nuclear AREG appears to affect the accumulation of key proteins in the nucleus and increase resistance to therapy in melanoma cells.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Tatjana Seitz, Nora John, Judith Sommer, Peter Dietrich, Wolfgang E. Thasler, Arndt Hartmann, Katja Evert, Sven A. Lang, Anja Bosserhoff, Claus Hellerbrand
Summary: Hepatic metastasis is a critical factor for cancer-related mortality. It has been found that fibroblast growth factor 9 (FGF9) released by hepatic stellate cells (HSCs) promotes the proliferation of uveal melanoma (UM) cells, indicating FGF9 as a potential therapeutic target for hepatic metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sebastian Staebler, Adrian Lichtblau, Slavyana Gurbiel, Thomas Schubert, Alexander Riechers, Ulrike Rottensteiner-Brandl, Anja Bosserhoff
Summary: MIA/CD-RAP is a protein secreted by chondrocytes and cartilaginous tissues. MIA/CD-RAP-deficient mice develop milder osteoarthritis. This study demonstrates that MIA/CD-RAP regulates MMP13 expression and activity, suggesting its potential as a new target for osteoarthritis therapy.
Article
Cell Biology
Agnes Soederberg, Tina Meissgeier, Anja Katrin Bosserhoff, Lisa Linck-Paulus
Summary: This study reveals high expression of MAGOH and MAGOHB proteins in cutaneous melanoma cell lines and patient derived tissue samples. Knockdown of MAGOH not only inhibits melanoma cell proliferation, but also induces apoptosis. Simultaneous knockdown of MAGOH and MAGOHB enhances this effect.
Article
Oncology
Tom Zimmermann, Sebastian Staebler, R. Verena Taudte, Sumeyya Uenuevar, Sabine Groesch, Stephanie Arndt, Sigrid Karrer, Martin F. Fromm, Anja-Katrin Bosserhoff
Summary: The exact mechanisms of action of cold atmospheric plasma (CAP) treatment for cancer are still unclear. This study found that CAP treatment caused a reduction in intracellular ceramide levels and a strong activation of the unfolded protein response (UPR) in melanoma cells. Additionally, pharmacological reduction in ceramides enhanced the effects of CAP treatment.
Article
Multidisciplinary Sciences
Isabel Stuefchen, Felix Beyer, Sebastian Staebler, Stefan Fischer, Melanie Kappelmann, Ruth Beckervordersandforth, Anja K. Bosserhoff
Summary: This study investigates the role of Sox9 in the melanocytic differentiation of adult Nestin* stem cells and finds that Sox9 is crucial for determining the fate of these cells. It reveals that Sox9 acts as a fate determinant that determines whether the cells differentiate into melanocytes or glial cells.
Article
Biochemistry & Molecular Biology
Vanessa Kersten, Tatjana Seitz, Judith Sommer, Wolfgang E. E. Thasler, Anja Bosserhoff, Claus Hellerbrand
Summary: Activated hepatic stellate cells (HSCs) are involved in hepatic fibrosis and hepatocarcinogenesis. This study shows that BMP13, expressed by HSCs, promotes the progression and growth of hepatocellular carcinoma (HCC). BMP13 acts through BMP type I receptors on HCC cells and targeting this growth factor could be a potential therapeutic strategy for HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Judith Sommer, Hanna Ehnis, Tatjana Seitz, Julia Schneider, Andreas B. Wild, Sandra Moceri, Christa Buechler, Aline Bozec, Georg F. Weber, Susanne Merkel, Ruth Beckervordersandforth, Alexander Steinkasserer, Roland Schuele, Jonel Trebicka, Arndt Hartmann, Anja Bosserhoff, Stephan von Hoersten, Peter Dietrich, Claus Hellerbrand
Summary: This study finds that obesity leads to inflammation in visceral adipose tissue, with macrophages playing a significant role. FHL2 expression is enhanced in VAT of obese individuals, and it is involved in the interaction between macrophages and adipocytes, contributing to the development of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)