Article
Biochemistry & Molecular Biology
Manuel Schmidt, Christine Poser, Christina Janster, Julia von Maltzahn
Summary: The hairpin region of WNT7A in its C-terminus is fully sufficient to induce non-canonical signaling in myogenic cells, leading to hypertrophy, expansion of satellite stem cells, and migration of myoblasts. The linker region in the C-terminus of WNT7A binds to the FZD7 receptor but does not activate non-canonical Wnt signaling. The hairpin and linker regions of WNT7A can independently activate canonical Wnt signaling.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Dymphna Margriet Ouwens, Michael Hewera, Guanzhang Li, Wang Di, Sajjad Muhammad, Daniel Haenggi, Hans-Jakob Steiger, Claudia A. Dumitru, Erol Sandalcioglu, Roland S. Croner, Wei Zhang, Or Kakhlon, Ulf D. Kahlert
Summary: Inhibiting canonical WNT signaling in GSCs functionally correlates with energy inhibition and glucose homeostasis. This correlation occurs in GSCs from different transcriptional or epigenetic transcriptional subtypes, suggesting that therapies directed against glycolysis/ATP-synthesis may be a promising strategy to overcome therapy resistance due to inter-tumoral heterogeneity and offers starting point to impair downstream signal WNT.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Cell Biology
Matthew J. Stevenson, Sabrina K. Phanor, Urvi Patel, Stephen S. Gisselbrecht, Martha L. Bulyk, Lori L. O'Brien
Summary: In this study, we found that the SIX1/2-Q177R mutation in Wilms tumors is associated with upregulation of WNT5A and other WNT pathway effectors. We demonstrated that SIX1 and SIX1-Q177R can activate the expression of WNT5A and have binding sites within the regulatory regions of WNT5A. These findings suggest that SIX1/2-Q177R may promote tumor progression and recurrence through dysregulation of the WNT signaling pathway.
DISEASE MODELS & MECHANISMS
(2023)
Article
Oncology
Wenli Xie, Naifu Liu, Xiangyu Wang, Ling Wei, Wenyan Xie, Xiugui Sheng
Summary: The study suggests that WTAP may promote chemo-resistance of EC cells to cisplatin through activation of the Wnt/beta-catenin pathway, offering novel insights into EC treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karina Lindbog Madsen, Oke Gerke, Poul F. Hoilund-Carlsen, Birgitte Brinkmann Olsen
Summary: Cancer stem cells (CSCs) are resistant to conventional therapy and responsible for cancer relapse. Auger electron emitters show promising potential in eradicating resistant lung cancer CD44(+) populations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Claudia Cantoni, Martina Serra, Erica Parisi, Bruno Azzarone, Angela Rita Sementa, Luigi Aurelio Nasto, Lorenzo Moretta, Giovanni Candiano, Cristina Bottino, Gian Marco Ghiggeri, Grazia Maria Spaggiari
Summary: The study reveals complex interactions between stromal-like Wilms tumor cells and NK cells, with activated NK cells efficiently degranulating upon contact with the cells, while the cells themselves produce inhibitory factors to limit NK cell activation and differentiation in the tumor microenvironment.
Article
Cell & Tissue Engineering
Hongwei Gao, Hui Dong, Jiachun Zheng, Xufeng Jiang, Mingzhi Gong, Le Hu, Jinshan He, Yongxiang Wang
Summary: The study identified LINC01119 as a negative regulator of osteogenic differentiation in MSCs, with its knockdown promoting calcification and osteogenic differentiation. It was revealed that LINC01119 negatively regulates osteogenesis through the Wnt pathway by targeting FZD4, with transcription factor EBF3 directly binding to LINC01119's promoter region.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Agriculture, Multidisciplinary
Cheng-Long Jin, Mao Ye, Zhi-Wen Song, Zong-Ming Zhang, Chun-Qi Gao, Hui-Chao Yan, Xiu-Qi Wang
Summary: This study revealed that lysine not only controls protein synthesis but also plays a role in regulating skeletal muscle growth. The expression of frizzled7 (FZD7) was positively correlated with lysine levels, and this was consistent with the activation of the Wnt/beta-catenin pathway. In addition, it was found that lysine binds to recombinant pig frizzled7 (rpFZD7) protein. This finding provides concrete evidence that lysine acts as a ligand for FZD7 to activate beta-catenin and stimulate muscle satellite cells (MuSCs) in promoting skeletal muscle growth.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Plant Sciences
Weijia Zhang, Chang Peng, Jiahui Yan, Pengting Chen, Cheng Jiang, Shuyi Sang, Yuemei Yuan, Yanjun Hong, Meicun Yao
Summary: This study demonstrated that the aqueous extract of Sanguisorba officinalis Linn. could inhibit the growth and metastasis of colorectal cancer cells by suppressing the Wnt pathway, providing potential for clinical application as an anti-CRC drug.
Article
Cell Biology
Qian Lan, Jiadong Cao, Xueting Bi, Xin Xiao, Dongsheng Li, Yilong Ai
Summary: This study suggests that using Cur-PDLSC-EV as a curcumin carrier can promote osteogenic differentiation of periodontal ligament stem cells, providing potential therapeutic value for periodontal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Shiki Fujino, Norikatsu Miyoshi, Aya Ito, Rie Hayashi, Masayoshi Yasui, Chu Matsuda, Masayuki Ohue, Masafumi Horie, Shinichi Yachida, Jun Koseki, Teppei Shimamura, Tsuyoshi Hata, Takayuki Ogino, Hidekazu Takahashi, Mamoru Uemura, Tsunekazu Mizushima, Yuichiro Doki, Hidetoshi Eguchi
Summary: High expression of POU5F1 is associated with colorectal cancer recurrence and liver metastasis. POU5F1-expressing cells in two-dimensional culture have cancer stem cell abilities and can form liver metastases in vivo. Single-cell RNA sequencing identifies heterogeneous populations derived from POU5F1-expressing cells and the Wnt signaling pathway is enriched in these cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oncology
Daniel J. Benedetti, Carly R. Varela, Lindsay A. Renfro, Brett Tornwall, David B. Dix, Peter F. Ehrlich, Richard D. Glick, John Kalapurakal, Elizabeth Perlman, Eric Gratias, Nita L. Seibel, James I. Geller, Geetika Khanna, Marcio Malogolowkin, Paul Grundy, Conrad V. Fernandez, Jeffrey S. Dome, Elizabeth A. Mullen
Summary: Pooled outcomes across three studies of patients with favorable histology Wilms tumor and extrapulmonary metastases revealed 4-year event-free survival of 77.3% and overall survival of 88.9%, with no statistical differences seen between patients on AREN0533 compared with those on NWTS-5. Missing details on local management of metastatic sites informs a critical need for better data capture in future studies to optimize local control strategies and chemotherapy regimens for this higher-risk favorable histology Wilms tumor patient group.
Review
Biochemistry & Molecular Biology
Jiajia Wang, Xichen Wan, Qihua Le
Summary: SOX9, a member of the SRY-related HMG-box transcription factors, plays a critical role in fetal development and stem cell homeostasis. The interactions between SOX9 and the canonical Wnt signalling pathway are involved in various physiological and pathological processes of stem cells, including organ development, stem cell proliferation, differentiation, and tumorigenesis. This review summarizes the molecular mechanism of cross-interactions between SOX9 and the canonical Wnt signalling pathway, outlines its regulatory effects on homeostasis in different types of stem cells, and explores its potential in translational stem cell therapy.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Angela Patricia Moreno-Londono, Maria Cristina Castaneda-Patlan, Miguel Angel Sarabia-Sanchez, Marina Macias-Silva, Martha Robles-Flores
Summary: The presence of cancer stem cells (CSCs) is associated with drug resistance and disease recurrence. This study aimed to investigate the role of the canonical Wnt/beta-catenin pathway in CSCs resistance to 5FU treatment. Using tumor spheroids as a model, the researchers found that activating the canonical Wnt pathway decreased 5FU-induced cell death, while inhibiting the pathway compromised the clonogenic capacity and stem cell markers expression. The combined treatment induced the survival of a small cell subpopulation that could recover and re-grow after treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)