Article
Engineering, Biomedical
Yu Xia, Guoyi Tang, Yi Chen, Changbing Wang, Min Guo, Tiantian Xu, Mingqi Zhao, Yongjian Zhou
Summary: RNA interference (RNAi) is a promising method for treating malignant tumors, and in this study, RGDfC-SeNPs were successfully synthesized as a tumor-targeted carrier for delivering siSox2 to HepG2 liver cancer cells. The results showed that RGDfC-Se@siSox2 effectively silenced Sox2, inhibited tumor cell proliferation, migration, and invasion, induced apoptosis through reactive oxygen species production and mitochondrial membrane potential disruption, and significantly inhibited tumor growth in mice bearing HepG2 tumors. These findings suggest that RGDfC-SeNPs could be a valuable gene carrier for specific gene-targeted therapy in HCC.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Francesca Perrone, Emanuela Fabiola Craparo, Maja Cemazar, Urska Kamensek, Salvatore Emanuele Drago, Barbara Dapas, Bruna Scaggiante, Fabrizio Zanconati, Debora Bonazza, Mario Grassi, Nhung Truong, Gabriele Pozzato, Rossella Farra, Gennara Cavallaro, Gabriele Grassi
Summary: The study demonstrates the valuable potential of PDPG for specific delivery of siRNAs targeting HCC-related genes, leading to reduced HCC cell vitality and downregulation of target gene expression.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Pharmacology & Pharmacy
Esinam E. Agbosu, Scott Ledger, Anthony D. Kelleher, Jing Wen, Chantelle L. Ahlenstiel
Summary: Our understanding of HIV infection has improved since its discovery in 1983, but a cure still remains elusive. The presence of a latent viral reservoir is a major barrier, and different strategies are being explored to eliminate or suppress it. RNA therapeutics, including siRNA and CRISPR gRNA, are potential options for achieving a functional cure. However, effective delivery into host cells remains a challenge for advancing RNA therapeutics to the clinic.
Review
Biochemistry & Molecular Biology
Sharad Khare, Tripti Khare, Raghu Ramanathan, Jamal A. Ibdah
Summary: This review discusses the role and involvement of microRNAs (miRs) in the heterogeneity and metastasis of hepatocellular carcinoma, with a special emphasis on their potential as diagnostic and prognostic tools in the risk prediction, early detection, and treatment of hepatocellular carcinoma.
Article
Chemistry, Multidisciplinary
Haohao Yin, Liping Sun, Yinying Pu, Jifeng Yu, Wei Feng, Caihong Dong, Bangguo Zhou, Dou Du, Yan Zhang, Yu Chen, Huixiong Xu
Summary: This study identified the activation of NFE2L2 during SDT, which hindered the treatment efficacy. A novel HMME @ Lip-Cas9 system was designed to enhance therapeutic efficiency, laying the groundwork for new approaches in HCC management.
ACS CENTRAL SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Minhee Kim, Su-Bin Kim, Jaehi Kim, Keun-Sik Kim, Dong-Eun Kim
Summary: This study examined the efficacy of combination therapy for hepatocellular carcinoma (HCC) using two types of hepatoma-targeting ligand-based liposomes to co-deliver curcumin and pituitary tumor modifying gene-1 (PTTG1) siRNA. The results showed that the combination treatment effectively inhibited tumor growth by suppressing the expression of Bcl-2 gene and increasing the expression of Caspase-3 gene in HCC. In the nude mouse model, intravenous injection of these liver-targeted liposome nano-pharmaceuticals effectively inhibited tumor growth. These results demonstrate the high potential of this combination therapy for HCC treatment.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Xuexiang Han, Ningqiang Gong, Lulu Xue, Margaret M. Billingsley, Rakan El-Mayta, Sarah J. Shepherd, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
Summary: By synthesizing AA-lipidoids with high delivery efficiency and selectivity, and validating in a preclinical model of liver fibrosis, the potential of AA-lipidoids for targeted RNA delivery to activated fibroblasts has been demonstrated.
NATURE COMMUNICATIONS
(2023)
Review
Oncology
Zhe Fan, Pengcheng Zhou, Binghui Jin, Guangyao Li, Lu Feng, Chengjun Zhuang, Shuang Wang
Summary: Hepatocellular carcinoma is a malignant tumor of hepatocytes that often has hidden presentation and rapid progression. Early stage HCC has no obvious symptoms and is usually diagnosed at an advanced stage, making it difficult to treat with only supportive and symptomatic treatment. Recent advancements in medical technology have led to the development of comprehensive treatment methods based on surgery.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Shubaash Anthiya, Suleyman Can Ozturk, Hamdullah Yanik, Ece Tavukcuoglu, Adem Sahin, Dhrubajyoti Datta, Klaus Charisse, David Moreira Alvarez, Maria Isabel Loza, Alfonso Calvo, Einar Sulheim, Simon Loevenich, Geir Klinkenberg, Ruth Schmid, Muthiah Manoharan, Gunes Esendagli, Maria Jose Alonso
Summary: The aim of this study was to develop a new therapeutic strategy for anti-KRAS siRNA through functionalized lipid nanoparticles (LNPs). A potent anti-KRAS siRNA sequence was chosen and tested for transfection efficacy and anti-proliferative effects. The selected siRNA was loaded into targeted and non-targeted LNPs, and their biodistribution and antitumoral efficacy were evaluated in a pancreatic cancer murine model. The results showed enhanced tumor accumulation and significant reduction in tumor growth with the targeted LNPs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Runze Li, Xiaohui Wu, Jing Li, Xuehe Lu, Robert Chunhua Zhao, Jianbing Liu, Baoquan Ding
Summary: This study presents a nanoplatform based on branched DNA aptamer clusters for efficient targeted drug delivery. The covalently conjugated branched DNA aptamer cluster-modified DNA tetrahedron demonstrates highly targeted cellular uptake, resulting in selective inhibition of tumor cell proliferation after loading the chemotherapeutic drug.
Article
Medicine, Research & Experimental
Tomasz Adamus, Chia-Yang Hung, Chunsong Yu, Elaine Kang, Mohamed Hammad, Linda Flores, Sergey Nechaev, Qifang Zhang, Joanna Marie Gonzaga, Kokilah Muthaiyah, Piotr Swiderski, Karen S. Aboody, Marcin Kortylewski
Summary: Neural stem cells (NSCs) can effectively transport antisense oligonucleotides (ASOs) targeting STAT3 protein to glioma microenvironment. NSC-derived exosomes loaded with CpG-STAT3ASO stimulate immune activity and inhibit tumor growth.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Review
Chemistry, Medicinal
Song Zhang, Zhujun Cheng, Yanan Wang, Tianyu Han
Summary: The progress of miRNA drugs in clinical trials is slow due to the characteristic of targeting multiple genes, leading to inevitable adverse effects.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Review
Immunology
Sihang Yu, Lei Zhou, Jiaying Fu, Long Xu, Buhan Liu, Yuanxin Zhao, Jian Wang, Xiaoyu Yan, Jing Su
Summary: There is increasing evidence showing the crucial role of the immune microenvironment, particularly the polarization state and function of macrophages, in the development of hepatocellular carcinoma. Tumor-derived exosomes in hepatocellular carcinoma act as information carriers and regulate the physiological state of cells in the microenvironment, thus controlling cancer progression. This review focuses on the role of exosome content in disease outcomes at different stages of hepatitis B virus/hepatitis C virus-induced hepatocellular carcinoma, and explores the mechanism by which macrophages contribute to hepatocellular carcinoma formation and the regulation of macrophage functions by the heterogeneity of exosome loading. The application prospects of exosome-based targeted drug delivery in immunotherapy research on hepatocellular carcinoma are also summarized.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yao Chen, Dong Zhao, Feng Xiao, Xuanyu Li, Jia'an Li, Zhenwei Su, Xingyu Jiang
Summary: In this study, a microfluidic chip was used to sequentially assemble multi-component nanoparticles, including therapeutic drugs and siRNA, with high encapsulation efficiencies and efficient tumor treatment effects.
ADVANCED MATERIALS
(2023)
Article
Oncology
Pengbo Cao, Qian Jin, Lan Feng, Haibei Li, Geng Qin, Gangqiao Zhou
Summary: The article introduces the roles of noncoding RNA (ncRNA) in hepatocellular carcinoma (HCC) and their potential clinical applications in cancer diagnosis and treatment, highlighting ncRNAs as potential novel biomarkers for cancer.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Cell Biology
Mayra Dominguez-Perez, Arturo Simoni-Nieves, Patricia Rosales, Natalia Nuno-Lambarri, Monica Rosas-Lemus, Veronica Souza, Roxana U. Miranda, Leticia Bucio, Salvador Uribe Carvajal, Jens U. Marquardt, Daekwan Seo, Luis E. Gomez-Quiroz, Maria Concepcion Gutierrez-Ruiz
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Review
Oncology
Stefania Recalcati, Margherita Correnti, Elena Gammella, Chiara Raggi, Pietro Invernizzi, Gaetano Cairo
FRONTIERS IN ONCOLOGY
(2019)
Article
Cell Biology
Oscar Bello-Monroy, Dulce Mata-Espinosa, Cristina Enriquez-Cortina, Veronica Souza, Roxana U. Miranda, Leticia Bucio, Jorge Barrios-Payan, Brenda Marquina-Castillo, Ignacio Rodriguez-Ochoa, Patricia Rosales, Maria Concepcion Gutierrez-Ruiz, Rogelio Hernandez-Pando, Luis Enrique Gomez-Quiroz
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Cell Biology
Pedro Posadas-Rodriguez, Natalia Esmeralda Posadas-Rodriguz, Viridiana Yazmin Gonzalez-Puertos, Rafael Toledo-Perez, Jose Luis Ventura-Gallegos, Alejandro Zentella, Luis Enrique Gomez-Quiroz, Mina Konigsberg, Armando Luna-Lopez
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2020)
Article
Oncology
Arturo Simoni-Nieves, Soraya Salas-Silva, Lisette Chavez-Rodriguez, Alejandro Escobedo-Calvario, Matthis Desoteux, Leticia Bucio, Veronica Souza, Roxana U. Miranda-Labra, Linda E. Munoz-Espinosa, Cedric Coulouarn, Maria Concepcion Gutierrez-Ruiz, Jens U. Marquardt, Luis E. Gomez-Quiroz
Summary: The study provides evidence that cholesterol is related to the development and aggressiveness of hepatocellular carcinoma, especially in western countries where high cholesterol diets are common. Cholesterol and high-fat diets are major causes of non-alcoholic fatty liver disease and NASH progression, which may also contribute to the development of HCC.
Review
Medicine, General & Internal
Carolin Czauderna, Martha M. Kirstein, Hauke C. Tews, Arndt Vogel, Jens U. Marquardt
Summary: Cholangiocarcinomas (CCAs) are a highly heterogeneous group of liver tumors with distinct prognostic and therapeutic traits. Specific treatment modalities are required for different subclasses, depending on anatomical localization and disease stage, to improve patient outcomes. Comprehensive molecular characterization has paved the way for precision oncological approaches in the treatment of CCAs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Oyku Gonul Geyik, Giulia Anichini, Engin Ulukaya, Fabio Marra, Chiara Raggi
Summary: Cholangiocarcinoma is a difficult to treat cancer with a rising incidence. Understanding its biology has been a hurdle to identify novel targets and effective treatments. Alterations in DNA damage response-related genes open up possibilities for DDR-targeting strategies. However, using DDR inhibitors alone may not be sufficient for clinical use, leading to consideration of combining them with DNA-damaging regimens and targeted drugs.
Article
Biochemistry & Molecular Biology
Monserrat Gerardo-Ramirez, Friederike L. Keggenhoff, Vanessa Giam, Diana Becker, Marco Groth, Nils Hartmann, Beate K. Straub, Helen Morrison, Peter R. Galle, Jens U. Marquardt, Peter Herrlich, Monika Hartmann
Summary: Osteosarcoma is the most common type of pediatric bone tumor. Research shows that CD44 plays an important role in drug resistance of osteosarcoma cells, and abnormal expression of CD44 leads to molecular alterations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Carlos Martinez-Armenta, Carlos Suarez-Ahedo, Anell Olivos-Meza, Maria C. Camacho-Rea, Laura E. Martinez-Gomez, Guadalupe Elizabeth Jimenez-Gutierrez, Gabriela A. Martinez-Nava, Luis E. Gomez-Quiroz, Carlos Pineda, Alberto Lopez-Reyes
Summary: In this study, a scaffold-free 3D culture system was developed to generate spheroids from human chondrocytes. The results showed that spheroids developed in a hypoxic environment exhibited better chondrogenesis characteristics compared to those developed under normoxic conditions. This novel culture system could be a valuable tool for cartilage biology research.
Article
Biochemistry & Molecular Biology
Mayrel Palestino-Dominguez, Alejandro Escobedo-Calvario, Soraya Salas-Silva, Moises Vergara-Mendoza, Veronica Souza-Arroyo, Roberto Lazzarini, Roxana Miranda-Labra, Leticia Bucio-Ortiz, Maria Concepcion Gutierrez-Ruiz, Luis E. Gomez-Quiroz
Summary: Alcohol-induced damage to the pancreas is a major risk factor for the development of pancreatitis. This study focused on the protective effect of hepatocyte growth factor (HGF) on pancreatic cells treated with ethanol and acetaldehyde. HGF reduced reactive oxygen species and lipid peroxidation, improved cell viability, and restored insulin expression impaired by alcohol metabolites.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Cell Biology
Ruediger Braun, Olha Lapshyna, Jessica Watzelt, Maren Drenckhan, Axel Kuenstner, Benedikt Faerber, Ahmed Ahmed Mohammed Hael, Louisa Bolm, Kim Christin Honselmann, Bjoern Konukiewitz, Darko Castven, Malte Spielmann, Sivahari Prasad Gorantla, Hauke Busch, Jens-Uwe Marquardt, Tobias Keck, Ulrich Friedrich Wellner, Hendrik Ungefroren
Summary: The prognosis of pancreatic ductal adenocarcinoma (PDAC) is very poor, and surgical resection is the only curative treatment option. A study established two new PDAC cell lines, which exhibit distinct molecular phenotypes, providing potential models for researching new treatment strategies and developing personalized therapy regimens.
Article
Oncology
Hendrik Ungefroren, Bjorn Konukiewitz, Rudiger Braun, Ulrich Friedrich Wellner, Hendrik Lehnert, Jens-Uwe Marquardt
Summary: Transcriptionally active p73 (TAp73) inhibits tumor progression in pancreatic ductal adenocarcinoma (PDAC) by promoting canonical TGF-beta/Smad signaling and preventing non-canonical ERK1/2-mediated TGF-beta signaling. TAp73 suppresses epithelial-mesenchymal transition and upregulates the expression of SMAD4, which mediates the inhibition of ERK activation and cell motility. Measuring the levels of TAp73 and/or SMAD4 could help predict the TGF-beta pathway preference in PDAC patients.
Review
Oncology
Benjamin Perez-Aguilar, Jens U. Marquardt, Encarnacion Munoz-Delgado, Rosa Maria Lopez-Duran, Maria Concepcion Gutierrez-Ruiz, Luis E. Gomez-Quiroz, Jose Luis Gomez-Olivares
Summary: The cholinergic system, specifically acetylcholine receptors (AChR) and acetylcholine (ACh), has been found to play a role in cancer development. Acetylcholinesterase (AChE), which regulates AChR by hydrolyzing ACh, has been observed to have a relevant role in cancer. However, there is controversy regarding AChE's participation in cancer, as its enzymatic activity can either increase or decrease in different tumors. This review focuses on analyzing the involvement of AChE in cancer progression and proposes AChE as a central regulator in the initiation and progression of cancer via the cholinergic system. Modulating ACh levels with AChE can regulate AChRs differentially, leading to diverse cancer events.
Review
Oncology
Jaqueline Quevedo-Ocampo, Alejandro Escobedo-Calvario, Veronica Souza-Arroyo, Roxana U. Miranda-Labra, Leticia Bucio-Ortiz, Maria C. Gutierrez-Ruiz, Lisette Chavez-Rodriguez, Luis E. Gomez-Quiroz
Summary: Cancer cells adapt their metabolic pathways to meet energy demands through accelerated proliferation. The folate cycle is crucial in providing the necessary metabolites for nucleotide synthesis, methylation, and reduction power. Its study is important in understanding cancer. Multiple enzymes in this cycle have been implicated in different types of cancer, confirming the adaptability of cancer cells under pathological conditions. Nonalcoholic fatty liver disease has emerged as a leading cause of hepatocellular carcinoma, particularly due to cholesterol accumulation. This review discusses the contribution of folate metabolism to cancer cells and tumor microenvironment, and the connection between cholesterol and the key enzyme methylenetetrahydrofolate dehydrogenase 1-like in the mitochondrial compartment.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2022)