期刊
ONCOGENE
卷 31, 期 15, 页码 1963-1974出版社
SPRINGERNATURE
DOI: 10.1038/onc.2011.373
关键词
EMT; DNA hypermethylation; WNT7A; WNT10A; oral squamous cell carcinoma
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Ministry of Health, Labour and Welfare, Japan
- Core Research for Evolutional Science and Technology of the Japan Science and Technology Corporation
- New Energy and Industrial Technology Development Organization
- Grants-in-Aid for Scientific Research [23650406, 22240090, 23390325, 24659888, 21592551, 22134002, 23390077] Funding Source: KAKEN
The epithelial-mesenchymal transition (EMT) has a crucial role in normal and disease processes including tumor progression. In this study, we first classified epithelial-like and mesenchymal-like oral squamous cell carcinoma (OSCC) cell lines based on expression profiles of typical EMT-related genes using a panel of 18 OSCC cell lines. Then, we performed methylation-based and expression-based analyses of components of the Wnt signaling pathway, and identified WNT7A and WNT10A as genes silenced by mesenchymal-specific DNA hypermethylation in OSCCs. A significant association was revealed between some clinicopathological findings and the DNA methylation status of WNT7A (normal vs tumor, P = 0.007; T1-2 vs T3-4, P = 0.040; I-III vs IV, P = 0.016) and WNT10A (N0-N1 vs N2-N3, P = 0.046) in the advanced stages of OSCC. Moreover, we found that E-cadherin expression in cancer cells may be positively regulated by WNT7A, whose expression is negatively regulated by mesenchymal-specific DNA hypermethylation or ZEB1 in mesenchymal-like OSCC cells. Our findings indicate that epithelial-specific gene silencing through mesenchymal-specific DNA hypermethylation may stabilize the phenotypic plasticity of cancer cells during EMT/MET. Oncogene (2012) 31, 1963-1974; doi:10.1038/onc.2011.373; published online 29 August 2011
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