Article
Cell Biology
Viktor Reiswich, Carol E. Schmidt, Maximilian Lennartz, Doris Hoeflmayer, Claudia Hube-Magg, Soren Weidemann, Christoph Fraune, Franziska Buescheck, Katharina Moeller, Christian Bernreuther, Ronald Simon, Till S. Clauditz, Niclas C. Blessin, Elena Bady, Guido Sauter, Ria Uhlig, Stefan Steurer, Sarah Minner, Eike Burandt, David Dum, Andreas H. Marx, Till Krech, Patrick Lebok, Andrea Hinsch, Frank Jacobsen
Summary: GATA3 is a transcription factor involved in cell differentiation and can be used as a diagnostic marker for breast and urothelial cancer. The study analyzed samples from 131 tumor types and subtypes and 76 normal tissue types, finding GATA3 positivity in various tumor entities. In breast cancer, low GATA3 levels were associated with advanced stage, high grade, hormone receptor negativity, and reduced survival.
Article
Cell Biology
Ramesh Elango, Sameera Rashid, Radhakrishnan Vishnubalaji, Reem Al-Sarraf, Mohammed Akhtar, Khalid Ouararhni, Julie Decock, Omar M. E. Albagha, Nehad M. M. Alajez
Summary: By performing transcriptomic profiling on breast cancer patients from Qatar, we identified differential gene and miRNA expression patterns based on molecular subtypes, tumor grade, patients' age, and ethnicity. We uncovered miRNA-mRNA regulatory networks and potential therapeutic targets, and validated mRNA and miRNA-based signatures predictive of relapse free survival.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Tomas Kupec, Andreas Bleilevens, Birgit Klein, Thomas Hansen, Laila Najjari, Julia Wittenborn, Elmar Stickeler, Jochen Maurer
Summary: This study investigated the expression of miRNAs in urine and serum of ovarian cancer patients using a human miRNA microarray chip. The findings suggest that miRNA profiling in urine may be more suitable than serum for identifying biomarkers in ovarian cancer.
Article
Biology
Ching-Te Kuo, Yu-Sheng Lai, Siang-Rong Lu, Hsinyu Lee, Hsiu-Hao Chang
Summary: Personalized drug cocktails have become popular in cancer treatment due to their use of drug combinations for better efficacy. In this study, a microcrater-arrayed (mu CA) chip was developed using laser direct writing to optimize cell amount and reagent usage. The results showed that the mu CA chip had better drug prediction accuracy compared to the conventional assay. This research has important implications for high-throughput drug screening and personalized medicine.
Article
Oncology
Jing Wang, Wang Dai, Ming Zhang
Summary: This study investigated the specific role and mechanism of GATA3 in neuroblastoma (NB) progression. The results showed that GATA3 positively regulates PAR1 to facilitate NB cell growth, migration, and invasion, while inhibiting the hippo pathway and decreasing sensitivity to cisplatin.
Article
Engineering, Biomedical
Nianzuo Yu, Feiran Zhang, Xiaoduo Tang, Yongshun Liu, Junhu Zhang, Bai Yang, Quan Wang
Summary: A microfluidic spotting-screening platform (MSSP) has been developed for controlling the effect of extracellular matrix on cell development. The MSSP can precisely control the behavior of mesenchymal stem cells, such as adhesion and differentiation, and provides a feasible and high-throughput platform for hydrogel-microarray-based materials.
ACTA BIOMATERIALIA
(2023)
Article
Pathology
Henning Plage, Henrik Samtleben, Sebastian Hofbauer, Kira Kornienko, Sarah Weinberger, Paul Giacomo Bruch, Sefer Elezkurtaj, Florian Rossner, Simon Schallenberg, Martina Kluth, Maximilian Lennartz, Niclas C. Blessin, Andreas H. Marx, Margit Fisch, Michael Rink, Marcin Slojewski, Krystian Kaczmarek, Thorsten Ecke, Steffen Hallmann, Stefan Koch, Nico Adamini, Sarah Minner, Ronald Simon, Guido Sauter, Tobias Klatte, Thorsten Schlomm, David Horst, Henrik Zecha
Summary: The expression of GATA3 protein in urothelial bladder cancer is not significantly correlated with pathological parameters and patient prognosis, but it is common in noninvasive urothelial carcinoma.
Article
Cell Biology
Zhigang Yang, Tao Xu, Tao Xie, Liangliang Yang, Guiping Wang, Yang Gao, Gangming Xi, Xiaobiao Zhang
Summary: This study investigated the role of CDC42EP3 in glioma development and its potential downstream mechanism. It was found that CDC42EP3 overexpression was positively correlated with pathological grading and increased the risk of recurrence. Knocking down CDC42EP3 inhibited cell proliferation and migration while promoting apoptosis. The study also suggested that CDC42EP3 might regulate the expression of CCND1. Overall, CDC42EP3 was found to promote glioma progression through targeting CCND1.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Bo Chen, Zhongyan Hua, Xiuni Qin, Zhijie Li
Summary: This study identified candidate genes and pathways related to neuroblastomas by analyzing NB miRNA and mRNA data. Through constructing PPI and miRNA-mRNA networks, three hub miRNAs were identified, with their target genes significantly enriched in multiple cancer-related signaling pathways.
NEUROCHEMICAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Sergey Lapa, Alexey Kuzmin, Larisa Hernousova, Vladimir Mikhailovich
Summary: The aim of this study was to develop a rapid PCR-based method for spoligotyping of Mycobacteria in the microarray format and compare it to conventional spoligotyping by hybridization. The method employs the on-Chip PCR technique with primers specific for 43 spacers in the DR region of mycobacterial DNA. The results showed that the on-Chip PCR method produced spoligotyping results that were in full concordance with the conventional reverse hybridization approach.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Silvia Uccella, Carla Facco, Anna Maria Chiaravalli, Fabiana Pettenon, Stefano La Rosa, Mario Turri-Zanoni, Paolo Castelnuovo, Michele Cerati, Fausto Sessa
Summary: In this study, the expression of SATB2 and GATA3 was investigated in sinonasal neuroendocrine neoplasms (SN-NENs). The results showed that SATB2 and GATA3 were features of Hyams' grades 1-3 olfactory neuroblastomas (ONBs), while they were negative in grade 4 ONBs and neuroendocrine carcinomas (NECs). This study expands the spectrum of SATB2 and GATA3-positive neoplasms and suggests biological differences between high grade and low graded ONBs.
ENDOCRINE PATHOLOGY
(2022)
Article
Chemistry, Analytical
Giorgio Ventimiglia, Massimiliano Pesaturo, Alastair Malcolm, Salvatore Petralia
Summary: A silicon lab-on-chip, integrating PCR and hybridization microarray, has been developed for nucleic acid detection. The lab-on-chip shows remarkable sensitivity, multiplexing analysis, and specificity for the detection of target sequences.
Article
Immunology
Ken-Ichi Suehiro, Akira Suto, Kensuke Suga, Hiroki Furuya, Arifumi Iwata, Taro Iwamoto, Shigeru Tanaka, Takahiro Kageyama, Kotaro Suzuki, Koichi Hirose, Veronique Lefebvre, Hiroshi Nakajima
Summary: Sox12 suppresses Th2 cell differentiation by accelerating Fbw7-mediated GATA3 degradation and attenuates HDM-induced allergic inflammation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Pathology
Sambit K. Mohanty, Preeti Diwaker, Sourav K. Mishra, Shilpy Jha, Anandi Lobo, Saroj P. Panda, Shivani Sharma, Mohit Kumar, Samriti Arora, Vipra Mallik, Deepika Jain, Ekta Jain, Indranil Chakrabarti, Juhi Varshney, Arshi Beg, Mallika Dixit, Manas R. Baisakh, Subhasini Naik, Subrat K. Sahoo, Mahmut Akgul, Bonnie L. Balzer, Mahul B. Amin, Anil V. Parwani
Summary: GATA3 and ISL1 expression were evaluated in pediatric small round blue cell tumors, and they were found to be useful in the diagnosis of neuroblastoma and differentiation from other tumors.
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tong Sun, Yu-jia Xu, Shuo-yi Jiang, Zhuan Xu, Bi-yin Cao, Gautam Sethi, Yuan-ying Zeng, Yan Kong, Xin-liang Mao
Summary: Recent studies have shown that the expression of CCND1 and the dysregulation of USP10 play crucial roles in glioma progression. Interactions between CCND1 and USP10 affect cell cycle control and apoptosis in GBM cells, highlighting their potential as therapeutic targets for GBM patients. The natural product acevaltrate (AVT) derived from Valeriana jatamansi Jones has shown promising effects in inducing GBM cell apoptosis by suppressing USP10-mediated deubiquitination on CCND1.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Hematology
Ellie Karampini, Petra E. Burgisser, Jenny Olins, Aat A. Mulder, Carolina R. Jost, Dirk Geerts, Jan Voorberg, Ruben Bierings
Summary: This study identified longin-SNARE Sec22b as a novel determinant of WPB size and VWF trafficking, depletion of which resulted in loss of elongated WPB morphology, Golgi disintegration, and VWF accumulation.
Article
Oncology
Lauren L. Fonseca, Won Seok Yang, Dirk Geerts, James Turkson, Junfang Ji, Joe W. Ramos
Summary: The study indicates that RasGRP1 is significantly elevated in human cSCC and can induce morphological changes and autophagy by increasing Ras activity. RasGRP1 is also shown to induce transformation in skin cells under certain conditions.
TRANSLATIONAL ONCOLOGY
(2021)
Review
Oncology
Kaylee M. Keller, Sonja Krausert, Apurva Gopisetty, Dan Luedtke, Jan Koster, Nil A. Schubert, Ana Rodriguez, Sander R. van Hooff, Damian Stichel, M. Emmy M. Dolman, Gilles Vassal, Stefan M. Pfister, Hubert N. Caron, Louis F. Stancato, Jan J. Molenaar, Natalie Jaeger, Marcel Kool
Summary: This study systematically reviewed the targeting of replication stress in pediatric tumors with a focus on DNA repair pathways and cell cycle checkpoint control. Various targets in these pathways have been studied in different diseases, with ATR, CHK1, PARP or WEE1 being the most promising targets in neuroblastoma, osteosarcoma, high-grade glioma or medulloblastoma.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Hematology
Marije Kat, Ellie Karampini, Arie J. Hoogendijk, Petra E. Burgisser, Aat A. Mulder, Floris P. J. Van Alphen, Jenny Olins, Dirk Geerts, Maartje Van den Biggelaar, Coert Margadant, Jan Voorberg, Ruben Bierings
Summary: In this study, the researchers identified Syntaxin 5 (STX5) as a novel regulator of Weibel-Palade body (WPB) biogenesis, showing that its depletion leads to decreased WPB length and Golgi fragmentation.
Article
Pharmacology & Pharmacy
Hong Im Kim, Chad R. Schultz, Gadisetti V. R. Chandramouli, Dirk Geerts, John Risinger, Andre S. Bachmann
Summary: Endometrial cancer (EC) is a common and deadly cancer in women, and the combination therapy of DFMO and GC7 shows potential in inhibiting tumor growth and reducing polyamine levels in EC cells.
JOURNAL OF DRUG TARGETING
(2022)
Article
Infectious Diseases
Tania Martins Garcia, Manon van Roest, Jacqueline L. M. Vermeulen, Sander Meisner, Jan Koster, Manon E. Wildenberg, Ruurd M. van Elburg, Vanesa Muncan, Ingrid B. Renes
Summary: This study investigated whether the effects of early life antibiotic use on the gut persist into adulthood and whether there are long-term consequences for gut functioning. The results showed that the majority of the antibiotic-induced gut effects did not persist into adulthood, but early life antibiotic use did impact later life gut functioning.
Article
Immunology
Ali Bashiri Dezfouli, Mina Yazdi, Mohamed-Reda Benmebarek, Melissa Schwab, Stefanos Michaelides, Arianna Micciche, Dirk Geerts, Stefan Stangl, Sarah Klapproth, Ernst Wagner, Sebastian Kobold, Gabriele Multhoff
Summary: This study presents a strategy to activate cellular immune response using heat shock protein 70 (Hsp70)-specific antibodies. By using the specific tumor surface antigen mHsp70, NK cells and T cells can be activated to exert cytotoxic effects against tumors. By constructing T cells with a specific anti-Hsp70 CAR, cell lifespan can be prolonged while maintaining their ability to recognize tumor cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kathleen Schoofs, Annouck Philippron, Francisco Avila Cobos, Jan Koster, Steve Lefever, Jasper Anckaert, Danny De Looze, Jo Vandesompele, Piet Pattyn, Katleen De Preter
Summary: This study conducted comprehensive RNA analysis of samples from esophageal adenocarcinoma patients, providing a unique resource for studying biomarkers and disease mechanisms. The dataset allows for comparison of tissue and liquid biopsy profiles, integration of coding and non-coding RNA patterns, and can serve as a validation dataset in other RNA landscaping studies.
Review
Biochemistry & Molecular Biology
Magali Belpaire, Arnaud Taminiau, Dirk Geerts, Rene Rezsohazy
Summary: This article summarizes the molecular data on the role of HOXA1 in breast cancer development over the past 25 years. Through large-scale patient cohort studies, the relationship between breast cancer subtypes and HOXA1 expression has been further clarified. These findings are of great significance for the understanding of breast cancer etiology and the evaluation of treatment opportunities.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Biochemistry & Molecular Biology
Jan Willem Bruggeman, Jan Koster, Ans M. M. van Pelt, Dave Speijer, Geert Hamer
Summary: This article proposes a hypothesis that activating the germline program promotes cancer cell malignancy. The four hallmark processes of the germline, including meiosis, epigenetic plasticity, migration, and metabolic plasticity, enable germ cells and cancer cells to have replicative immortality. Especially, meiotic genes are frequently expressed in cancer, suggesting their potential role in oncogenesis.
Article
Oncology
Sima Ardalan Khales, Sina Mozaffari-Jovin, Dirk Geerts, Mohammad Reza Abbaszadegan
Summary: This study reveals the crucial role of TWIST1 in the establishment of a cancer stem cell-like phenotype, EMT progression, and inhibition of apoptosis in esophageal squamous cell carcinoma (ESCC). These findings provide evidence for TWIST1 as a therapeutic target for suppressing CSC generation and ESCC malignancy.
Article
Chemistry, Medicinal
Catherine M. Mills, Jonathan Turner, Ivett C. Pina, Kathleen A. Garrabrant, Dirk Geerts, Andre S. Bachmann, Yuri K. Peterson, Patrick M. Woster
Summary: LSD1, an epigenetic regulator, is an oncogenic accomplice in MYCN-expressing neuroblastoma. We report 3 novel scaffolds for reversible LSD1 inhibition and found one of them to be highly potent and able to increase an important modification mark in cells. Combination treatment with bortezomib also showed a synergistic effect.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ton Schoenmaker, Joy Zwaak, Bruno G. Loos, Richard Volckmann, Jan Koster, E. Marelise W. Eekhoff, Teun J. de Vries
Summary: Fibrodysplasia Ossificans Progressiva (FOP) is a rare genetic disease characterized by progressive heterotopic ossification that leads to immobility and premature death. It is caused by a mutation in the ACVR1 gene, resulting in altered response to Activin-A. Activin-A induces changes in osteoclastogenesis at the gene expression level, regardless of the presence of the mutated ACVR1 receptor. RNA sequencing analysis revealed differentially expressed genes and enriched pathways associated with osteoclast differentiation, cell fusion, and inflammation. These findings suggest that Activin-A plays a significant role in gene expression during osteoclast formation in FOP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Chad R. Schultz, Ryan D. Sheldon, Huirong Xie, Elena Y. Demireva, Katie L. Uhl, Dalen W. Agnew, Dirk Geerts, Andre S. Bachmann
Summary: Researchers have generated new genome-edited mouse models using CRISPR-Cas9 to study the important proteins eIF5A1 and eIF5A2, which are involved in various physiological and pathophysiological processes. These models have a mutation that prevents the formation of a key lysine derivative called hypusine, leading to altered metabolite landscape and potential implications for diseases and therapies.
Meeting Abstract
Oncology
Sai Preethi Nakkina, Sarah B. Gitto, Veethika Pandey, Jignesh Parikh, Dirk Geerts, Carlo Maurer, Kenneth P. Olive, Otto Phanstiel, Deborah A. Altomare