Article
Biochemistry & Molecular Biology
Natalia Dos Santos Goncalves, Tamires Maria Silva Pereira de Mello, Cassia Suemi Mizuno, Saqlain Haider, Raquel Alves Dos Santos
Summary: The study evaluated the cytotoxicity and antiproliferative effects of cis- and trans-TMS on MCF-7 and MCF-10A cells, showing both compounds exhibited cytotoxicity, genotoxicity, and affected cell cycle progression and cell death. Notably, the antiproliferative effects were more pronounced for cis-TMS compared to trans-TMS.
GENETICS AND MOLECULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Bisan Samara, Muhammedin Deliorman, Pavithra Sukumar, Mohammad A. Qasaimeh
Summary: A method of creating cryopreservable high throughput arrays of 3D tumor models using a paper platform is presented, utilizing virtual microwells to facilitate cell aggregation and prevent mixing, allowing for extended storage and drug treatment studies.
Article
Obstetrics & Gynecology
Hye In Kim, Inha Lee, Yeon Soo Jung, Seung Joo Chon, Bo Hyon Yun, Seok Kyo Seo
Summary: Korean red ginseng extract inhibits growth and induces apoptosis of breast cells, potentially reducing the risk of breast cancer in postmenopausal women.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2021)
Article
Oncology
Noemi Monti, Simona Dinicola, Alessandro Querqui, Gianmarco Fabrizi, Valeria Fedeli, Luisa Gesualdi, Angela Catizone, Vittorio Unfer, Mariano Bizzarri
Summary: Epithelial-Mesenchymal Transition (EMT) causes the transformation of epithelial cells into a mesenchymal-like phenotype, leading to loss of cell-to-cell contact and increased motility/invasive capabilities. This process is important in inflammation and cancer progression, driven by factors such as TGF-beta 1. However, myo-inositol can reverse the EMT process induced by TGF-beta 1 and restore the epithelial phenotype.
Article
Biochemistry & Molecular Biology
Dilek Bayram, Meltem ozgocmen, Dilek Asci celik, Emine Sarman, Murat Sevimli
Summary: Breast cancer, originating in the lobules or ducts of the breast, is the second leading cause of death among women worldwide. Studies have been conducted to find the optimal treatment for breast cancer, and boric acid is considered as a potential anti-cancer agent for treating this disease.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Cell Biology
Kamila Bialkowska, Piotr Komorowski, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Maria Bryszewska, Katarzyna Milowska
Summary: Cationic dendrimers have been found to be effective carriers for delivering siRNA into cells and protecting nucleic acids. In 3D cell cultures, CBD-2 exhibits higher cytotoxicity compared to CBD-1, while the opposite is observed in 2D cultures. Attaching siRNA to a dendrimer molecule does not increase cellular toxicity. CBD-2 shows higher efficiency in transporting siRNAs compared to CBD-1.
Article
Biophysics
Sen Liu, Haojun Liang, Linwen Lv, Fan Hu, Qiuyang Liu, Yujiao Wang, Junyu Zhu, Ziteng Chen, Jiacheng Li, Zhijie Wang, Ya-nan Chang, Juan Li, Xiancai Ma, Kui Chen, Gengmei Xing
Summary: This study found that the polyhydroxylated fullerenol nanoparticle (FNP) can induce the exposure of calreticulin (CRT) on the surface of MCF-7 cells by redistributing CRT from the endoplasmic reticulum (ER). The combination of FNP and anti-CD47 monoclonal antibody (mAb) enhances macrophage-mediated phagocytosis of cancer cells. In vivo experiments also confirmed the regulatory effect of FNP on the progression of MCF-7 cancer stem-like cells (CSCs). These findings expand the application of FNP in tumor therapy with anti-CD47 mAb and suggest the use of 3D culture as a screening tool for nanomedicine.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Xu-Dong Mao, Ting -Ting Du, Qi Gu, Li Yang, Hai-Lian Shi, Ran Hong, Gui-Xin Chou
Summary: Nepetaefolin F, a compound, exhibited significant inhibition against human cancer cells with an IC50 value of 6.3 μM. The synthesis of Nepetaefolin F and its analogues were performed, and one analogue, cyclopropanecarboxylate ester 42, showed significant antitumor activity against MGC 803 cells with an IC50 value of 20.9 μM. Further studies revealed that compound 42 could regulate multiple signaling pathways related to autophagy, such as PPAR and AMPK, and induce cell apoptosis in MGC 803 cells by inhibiting cell proliferation and autophagy.
Article
Chemistry, Multidisciplinary
Xu-Dong Mao, Ting-Ting Du, Qi Gu, Li Yang, Hai-Lian Shi, Ran Hong, Gui-Xin Chou
Summary: Nepetaefolin F is a natural compound that has shown significant inhibitory activity against human cancer cells, making it a promising candidate for cancer treatment.
Article
Oncology
Yongqiang Chen, Ruobing Wang, Shujun Huang, Elizabeth S. Henson, Jayce Bi, Spencer B. Gibson
Summary: ERBB2 overexpression in cancer cells leads to drug resistance, with autophagy being one of the mechanisms involved. ERBB2 promotes autophagy by upregulating the expression of the autophagy gene ATG12, contributing to resistance to chemotherapy drugs or ERBB2-targeted antibody treatments. ATG12 expression in ERBB2-positive breast tumors correlates with a worse patient survival outcome. Lapatinib, an inhibitor for both EGFR and ERBB2 tyrosine kinases, has differential effects on autophagy in cells expressing only EGFR versus only ERBB2.
Article
Biology
Hareth Y. ShujaaEdin, Nagi A. AL-Haj, Abdullah Rasedee, Noorjahan Banu Alitheen, Arifah Abdul Kadir, Chee Wun How, Heshu Sulaiman Rahman, Al-Shwyeh Hussah Abdullah
Summary: The study found that rHuEPO had a greater effect on the viability of MCF-7 cells in 3D culture, significantly reducing cell viability and down-regulating caspase activity. Cell cycle analysis showed that rHuEPO caused the MCF-7 cells to enter the subG0/G1 phase.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Natalia Zareba, Klaudia Wieclawik, Rene Kizek, Bozena Hosnedlova, Marta Kepinska
Summary: This study synthesized C-60-DOX complexes and investigated their effects on the concentrations of MT and SOD, as well as the activity of SOD in MCF-10A cells. The results showed that the C-60-DOX complexes exhibited protective effects against DOX-induced toxicity in MCF-10A cells.
Article
Oncology
Qilong Ma, Dinghao Zhuo, Feng Guan, Xiang Li, Xiaomin Yang, Zengqi Tan
Summary: The study found that GM1-enriched sEVs derived from breast cancer cells can induce the epithelial-mesenchymal transition (EMT) process in recipient cells. Proteomic analysis revealed differentially expressed proteins related to sEV secretion and identified oncogenic differentially expressed proteins presented in sEVs.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Xiaoyang Liu, Sandhya Chipurupalli, Peijia Jiang, Mahtab Tavasoli, Byong Hoon Yoo, Michael McPhee, Sina Mazinani, Giulio Francia, Robert S. Kerbel, Kirill Rosen
Summary: We discovered a novel mechanism of ErbB2-driven 3D breast tumor growth mediated by ErbB2-dependent BLNK downregulation. In addition, we found that upregulating BLNK can inhibit tumor growth, and increased mRNA levels of BLNK are associated with increased relapse-free survival.
CELL DEATH & DISEASE
(2022)
Article
Plant Sciences
Bayram Dilek, Ozgocmen Meltem
Summary: Breast cancer, the second leading cause of death and the most common type of cancer in women worldwide, can potentially be treated with the flavonoid quercetin, which induces apoptosis and inhibits proliferation of breast cancer cells.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Yasuhiro Saito, Ridhdhi R. Desai, Senthil K. Muthuswamy
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2018)
Editorial Material
Cell & Tissue Engineering
Senthil K. Muthuswamy
Article
Biophysics
Robert J. Natividad, Mark L. Lalli, Senthil K. Muthuswamy, Anand R. Asthagiri
BIOPHYSICAL JOURNAL
(2018)
Article
Multidisciplinary Sciences
Yasuhiro Saito, Lewyn Li, Etienne Coyaud, Augustin Luna, Chris Sander, Brian Raught, John M. Asara, Myles Brown, Senthil K. Muthuswamy
Article
Cell Biology
SungHee Park, Mattia Brugiolo, Martin Akerman, Shipra Das, Laura Urbanski, Adam Geier, Anil K. Kesarwani, Martin Fan, Nathan Leclair, Kuan-Ting Lin, Leo Hu, Ian Hua, Joshy George, Senthil K. Muthuswamy, Adrian R. Krainer, Olga Anczukow
Article
Oncology
Spas Dimitrov-Markov, Javier Perales-Paton, Bruno Bockorny, Ana Dopazo, Manuel Munoz, Natalia Banos, Victoria Bonilla, Camino Menendez, Yolanda Duran, Ling Huang, Sofia Perea, Senthil K. Muthuswamy, Fatima Al-Shahrour, Pedro P. Lopez-Casas, Manuel Hidalgo
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Medicine, Research & Experimental
Ling Huang, Bruno Bockorny, Indranil Paul, Dipikaa Akshinthala, Pierre-Oliver Frappart, Omar Gandarilla, Arindam Bose, Veronica Sanchez-Gonzalez, Emily E. Rouse, Sylvain D. Lehoux, Nicole Pandell, Christine M. Lim, John G. Clohessy, Joseph Grossman, Raul Gonzalez, Sofia Perea Del Pino, George Daaboul, Mandeep S. Sawhney, Steven D. Freedman, Alexander Kleger, Richard D. Cummings, Andrew Emili, Lakshmi B. Muthuswamy, Manuel Hidalgo, Senthil K. Muthuswamy
Article
Multidisciplinary Sciences
Tulin Dadali, Anne R. Diers, Shiva Kazerounian, Senthil K. Muthuswamy, Pallavi Awate, Ryan Ng, Saie Mogre, Carrie Spencer, Katerina Krumova, Hannah E. Rockwell, Justice McDaniel, Emily Y. Chen, Fei Gao, Karl T. Diedrich, Vijetha Vemulapalli, Leonardo O. Rodrigues, Viatcheslav R. Akmaev, Khampaseuth Thapa, Manuel Hidalgo, Arindam Bose, Vivek K. Vishnudas, A. James Moser, Elder Granger, Michael A. Kiebish, Stephane Gesta, Niven R. Narain, Rangaprasad Sarangarajan
Summary: Reactive oxygen species (ROS) play a role in triggering cell signaling events to promote and maintain tumorigenicity. Chemotherapy and radiation can induce ROS to elicit cell death, making it possible to target ROS pathways for effective anti-cancer therapeutics. Coenzyme Q(10) is shown to increase ROS generation when delivered to increase mitochondrial Q-pool, resulting in anti-cancer effects in a pancreatic cancer model.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Oncology
Senthil K. Muthuswamy
Summary: Pathologists classify tumors based on histological features and determine the site of origin for metastasis. The mechanisms behind how tumors organize themselves and recreate their histological structure during metastasis remain unknown. The concept of "histostasis" is discussed as a potential explanation for tumors possessing a histological memory and its implications for metastasis.
Article
Cell & Tissue Engineering
Ling Huang, Ridhdhi Desai, Daniel N. Conrad, Nayara C. Leite, Dipikaa Akshinthala, Christine Maria Lim, Raul Gonzalez, Lakshmi B. Muthuswamy, Zev Gartner, Senthil K. Muthuswamy
Summary: Induction of human pluripotent stem cells towards pancreatic ductal and acinar organoids has provided a renewable source for modeling exocrine development and diseases. The study demonstrated the lineage tropism and plasticity for oncogene action in the human pancreas, with specific oncogenes like GNAS(R201C) and KRAS(G12D) showing differential effects on ductal and acinar organoids both in culture and in vivo.
Article
Biochemistry & Molecular Biology
Kazuhiro Koikawa, Shin Kibe, Futoshi Suizu, Nobufumi Sekino, Nami Kim, Theresa D. Manz, Benika J. Pinch, Dipikaa Akshinthala, Ana Verma, Giorgio Gaglia, Yutaka Nezu, Shizhong Ke, Chenxi Qiu, Kenoki Ohuchida, Yoshinao Oda, Tae Ho Lee, Babara Wegiel, John G. Clohessy, Nir London, Sandro Santagata, Gerburg M. Wulf, Manuel Hidalgo, Senthil K. Muthuswamy, Masafumi Nakamura, Nathanael S. Gray, Xiao Zhen Zhou, Kun Ping Lu
Summary: Upregulation of Pin1 in PDAC contributes to the desmoplastic and immunosuppressive TME, but targeting Pin1 with available drugs can synergize with immunotherapy and gemcitabine to induce complete elimination of PDAC.
Article
Multidisciplinary Sciences
Shawn C. Chafe, Frederick S. Vizeacoumar, Geetha Venkateswaran, Oksana Nemirovsky, Shannon Awrey, Wells S. Brown, Paul C. McDonald, Fabrizio Carta, Andrew Metcalfe, Joanna M. Karasinska, Ling Huang, Senthil K. Muthuswamy, David F. Schaeffer, Daniel J. Renouf, Claudiu T. Supuran, Franco J. Vizeacoumar, Shoukat Dedhar
Summary: The study identified a redox homeostasis network involving the iron-sulfur cluster enzyme NFS1 in the survival mechanisms governed by the tumor hypoxia-induced pH regulator CAIX. Targeting CAIX while depleting NFS1 or blocking cyst(e)ine availability enhanced ferroptosis and inhibited tumor growth significantly. Altering intracellular pH and iron homeostasis through inhibiting CAIX activity may lead to the development of innovative therapeutic strategies for solid tumors to overcome hypoxia- and acidosis-mediated tumor progression and therapeutic resistance.
Article
Oncology
Joseph E. Grossman, Lakshmi Muthuswamy, Ling Huang, Dipikaa Akshinthala, Sofia Perea, Raul S. Gonzalez, Leo L. Tsai, Jonah Cohen, Bruno Bockorny, Andrea J. Bullock, Benjamin Schlechter, Mary Linton B. Peters, Catherine Conahan, Supraja Narasimhan, Christine Lim, Roger B. Davis, Robert Besaw, Mandeep S. Sawhney, Douglas Pleskow, Tyler M. Berzin, Martin Smith, Tara S. Kent, Mark Callery, Senthil K. Muthuswamy, Manuel Hidalgo
Summary: This pilot feasibility trial investigated the use of patient-derived organoids (PDOs) to guide personalized therapy for pancreatic ductal adenocarcinoma (PDAC). The study found that the drug sensitivity of PDOs can predict the clinical outcome of patients and is correlated with treatment responses.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Qingda Meng, Shanshan Xie, G. Kenneth Gray, Mohammad H. Dezfulian, Weilin Li, Ling Huang, Dipikaa Akshinthala, Elizabeth Ferrer, Catherine Conahan, Sofia Perea Del Pino, Joseph Grossman, Stephen J. Elledge, Manuel Hidalgo, Senthil K. Muthuswamy
Summary: This study reports a platform for expanding tumor-targeting T cells from the peripheral blood of patients with pancreatic cancer, and identifies the NKG2A-HLA-E axis as an important checkpoint for CD8+ T cells in pancreatic cancer. Empirical identification of tumor-targeting TCRs suitable for TCR therapy was demonstrated.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Endocrinology & Metabolism
Sevim Kahraman, Ercument Dirice, Giorgio Basile, Danielle Diegisser, Jahedul Alam, Bente B. Johansson, Manoj K. Gupta, Jiang Hu, Ling Huang, Chew-Li Soh, Danwei Huangfu, Senthil K. Muthuswamy, Helge Raeder, Anders Molven, Rohit N. Kulkarni
Summary: The interaction between exocrine and endocrine cells through the transfer of mutant CEL protein from pancreatic acinar cells to beta-cells causes dysfunction and loss of beta-cells, leading to the development of MODY8.