Article
Biochemistry & Molecular Biology
Regina F. Fernandez, Emily S. Wilson, Victoria Diaz, Jonatan Martinez-Gardeazabal, Rachel Foguth, Jason R. Cannon, Shelley N. Jackson, Brian P. Hermann, Jeffrey B. Eells, Jessica M. Ellis
Summary: Deleting the 6th isoform of long-chain acyl-CoA synthetase (ACSL6) in mice alters dopamine metabolism and regulation of light entrainment, suggesting that DHA metabolism mediated by ACSL6 plays a crucial role in dopamine neuron biology.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Hiroki Hashizume, Tatsuki Fukami, Kanji Mishima, Hiroshi Arakawa, Kenji Mishiro, Yongjie Zhang, Masataka Nakano, Miki Nakajima
Summary: This study aimed to clarify the human ACS isoforms responsible for CoA-conjugation of NSAIDs by considering the hepatic expression levels of ACS isoforms. Among the 10 types of NSAIDs studied, propionic acid-class NSAIDs were found to be conjugated with CoA in the human liver, while NSAIDs in other classes did not exhibit this reaction. ACSL1 was identified as the most highly expressed ACS isoform in the human liver, and could catalyze the CoA conjugation of propionic acid-class NSAIDs, which may lead to toxicity through protein adduct formation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Sport Sciences
Harrison D. Stierwalt, Sarah E. Ehrlicher, Matthew M. Robinson, Sean A. Newsom
Summary: This study found that most ACSL protein isoforms can be detected in human skeletal muscle, with minimal changes in abundance after acute exercise. ACSL1 and ACSL6 are possible determinants of fat oxidation and fat storage within skeletal muscle, confirming findings from model systems.
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
(2021)
Article
Biology
Yu Meng, Cheryl Ingram-Smith, Oly Ahmed, Kerry Smith
Summary: By investigating the roles of active site residues in CoA binding in acetyl-CoA synthetase (Acs) and a medium-chain acyl-CoA synthetase (Macs), it was found that certain residues play a critical role in CoA binding and catalysis, revealing the active site architecture of this important enzyme superfamily.
Article
Biochemistry & Molecular Biology
Yuki Tomitsuka, Hiroki Imaeda, Haruka Ito, Isaki Asou, Masayuki Ohbayashi, Fumihiro Ishikawa, Hiroshi Kuwata, Shuntaro Hara
Summary: Long-chain acyl-CoA synthetase 4 (ACSL4) converts polyunsaturated fatty acids (PUFAs) into their acyl-CoAs and plays a crucial role in maintaining PUFA-containing membrane phospholipids. In this study, ACSL4 deficiency was found to attenuate pulmonary toxic chemical-induced lung injury and reduce mortality in mice. The results also showed that ACSL4 deficiency suppressed inflammation and neutrophil migration, as well as lipid peroxidation in the lung.
Review
Plant Sciences
Huayan Zhao, Dylan K. Kosma, Shiyou Lu
Summary: Fatty acids play crucial roles in plants as components of lipid membranes, sources of energy, and signaling molecules. Changes in LACS activity can lead to pleiotropic phenotypes. This review provides a comprehensive analysis of LACS family enzymes and highlights areas for future research.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Olivia A. Maguire, Sarah E. Ackerman, Sarah K. Szwed, Aarthi V. Maganti, Francois Marchildon, Xiaojing Huang, Daniel J. Kramer, Adriana Rosas-Villegas, Rebecca G. Gelfer, Lauren E. Turner, Victor Ceballos, Asal Hejazi, Bozena Samborska, Janane F. Rahbani, Christien B. Dykstra, Matthew G. Annis, Ji-Dung Luo, Thomas S. Carroll, Caroline S. Jiang, Andrew J. Dannenberg, Peter M. Siegel, Sarah A. Tersey, Raghavendra G. Mirmira, Lawrence Kazak, Paul Cohen
Summary: The study identified the key role of crosstalk between mammary adipocytes and neoplastic cells in promoting obesity-driven breast cancer progression. Glycine amidinotransferase (Gatm) in adipocytes and Acsbg1 in cancer cells were found to be crucial for this process.
Review
Pharmacology & Pharmacy
Jun Hou, Changqing Jiang, Xudong Wen, Chengming Li, Shiqiang Xiong, Tian Yue, Pan Long, Jianyou Shi, Zhen Zhang
Summary: Cancer is a major global health problem, and ACSL4 has different roles in different cancer cells, both inhibiting cancer cell progression and potentially being associated with tumor cell proliferation, migration, and invasion.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Romain Marteau, Severine Ravez, Darius Mazhari Dorooee, Hind Bouchaoui, Karine Porte, Jean-Christophe Devedjian, Patricia Melnyk, David Devos, Raphael Frederick, Jamal El Bakali
Summary: Ferroptosis is an iron-dependent regulated cell death characterized by the accumulation of lipid peroxides, and recent studies have shown that ACSL4 inhibitors are emerging as attractive agents for anti-ferroptosis. Screening FDA-approved drug library has identified new inhibitors with micromolar-range activities against ACSL4, with sertaconazole being the most potent inhibitor with an IC50 of 280 nM against hACSL4.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Holly Dykstra, Chelsea Fisk, Cassi LaRose, Althea Waldhart, Xing Meng, Gongpu Zhao, Ning Wu
Summary: This study characterized the mammalian ACSL1 protein and demonstrated its activity as a monomer through enzymatic assays, mutational analysis, and cryo-electron microscopy.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Runyi Huang, Wenli Yu, Rongzhen Zhang, Yan Xu
Summary: This study developed an efficient method for producing malonyl-CoA by screening the ACS gene from Streptomyces sp. The newly characterized ACS enzyme showed high conversion rate and yield of malonyl-CoA under optimized conditions.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hiroshi Kuwata, Yuki Tomitsuka, Emiko Yoda, Shuntaro Hara
Summary: In this study, ACSL4 knockdown was found to decrease the levels of PUFA-derived acyl-CoA, particularly leading to a decrease in DHA-containing phospholipids. This inhibition further suppressed cell death induced by ferroptosis.
BIOSCIENCE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Anthony H. Futerman, Jiyoon L. Kim, Beatriz Mestre, Sergey Malitsky, Maxim Itkin, Meital Kupervaser
Summary: This study reveals the interaction between the FATP family and ceramide synthases, highlighting the importance of manipulating fatty acyl-CoA generation pathway in lipid metabolism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Mengmeng Zheng, Jun Zhang, Wan Zhang, Lu Yang, Xiaoli Yan, Wenya Tian, Zhihao Liu, Zhi Lin, Zixin Deng, Xudong Qu
Summary: In this study, an ACS enzyme was improved through protein engineering, leading to enhanced activity in synthesizing acyl-CoAs. By combining it with carboxylases, several novel antimycin analogues were successfully produced through a modified biosynthetic pathway. This study expands the catalytic mode of ACSs and provides an important tool for the biosynthesis of acyl-CoA-derived natural products.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Chiaki Fujiwara, Yukiko Muramatsu, Megumi Nishii, Kazuhiro Tokunaka, Hidetoshi Tahara, Masaru Ueno, Takao Yamori, Yoshikazu Sugimoto, Hiroyuki Seimiya
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Tomohisa Hatta, Shun-ichiro Lemura, Tomokazu Ohishi, Hiroshi Nakayama, Hiroyuki Seimiya, Takao Yasuda, Katsumi Iizuka, Mitsunori Fukuda, Jun Takeda, Tohru Natsume, Yukio Horikawa
SCIENTIFIC REPORTS
(2018)
Review
Cell Biology
Keiji Okamoto, Hiroyuki Seimiya
Editorial Material
Biochemistry & Molecular Biology
Keiji Okamoto, Hiroyuki Seimiya
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Anna Mizutani, Hiroyuki Seimiya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Oncology
Ryuhei Kawakami, Tetsuo Mashima, Naomi Kawata, Koshi Kumagai, Toshiro Migita, Takeshi Sano, Nobuyuki Mizunuma, Kensei Yamaguchi, Hiroyuki Seimiya
Article
Oncology
Myung-Kyu Jang, Tetsuo Mashima, Hiroyuki Seimiya
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Chuya Nakanishi, Hiroyuki Seimiya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Fumiya Tomizawa, Myung-Kyu Jang, Tetsuo Mashima, Hiroyuki Seimiya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Review
Oncology
Hiroyuki Seimiya
Article
Biochemistry & Molecular Biology
Kunikazu Tanji, Fumiaki Mori, Fumiyuki Shirai, Takehiro Fukami, Hiroyuki Seimiya, Jun Utsumi, Akiyoshi Kakita, Koichi Wakabayashi
Summary: Research has shown that tankyrase inhibitors can suppress the formation of TDP-43 protein aggregates and reduce the levels of tankyrase protein in neuronal cytoplasmic inclusions, potentially protecting against TDP-43 toxicity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Kyoko Matsumoto, Keiji Okamoto, Sachiko Okabe, Risa Fujii, Koji Ueda, Kenichi Ohashi, Hiroyuki Seimiya
Summary: G-quadruplex (G4) can suppress ISG expression in cancer cells by binding to splicing factor 3B subunit 2 (SF3B2), providing a new mode for gene regulation.
Review
Biotechnology & Applied Microbiology
Hiroyuki Seimiya, Kazuo Nagasawa, Kazuo Shin-ya
Summary: G-quadruplexes (G4s) are higher-order structures formed by guanine-rich sequences of nucleic acids, regulating biological events and associated with diseases like cancer. Telomestatin, a natural compound, stabilizes G4 structures and inhibits telomerase, potentially leading to anticancer effects. Synthetic derivatives have been developed to overcome the limitations of the natural compound and show promise in preclinical cancer models. Further research on G4-stabilizing compounds as potential cancer therapeutics and the identification of predictive biomarkers for drug efficacy are critical for future clinical studies.
JOURNAL OF ANTIBIOTICS
(2021)
Review
Oncology
Caroline Moyret-Lalle, Melanie K. Prodhomme, Delphine Burlet, Ayaka Kashiwagi, Virginie Petrilli, Alain Puisieux, Hiroyuki Seimiya, Agnes Tissier
Summary: EMT is involved in tumor development and DNA repair. Understanding the role of EMT in DNA repair can lead to new treatments targeting tumors and improving efficacy.
Article
Chemistry, Multidisciplinary
Mizuho Yasuda, Yue Ma, Sachiko Okabe, Yuki Wakabayashi, Dongdong Su, Young-Tae Chang, Hiroyuki Seimiya, Masayuki Tera, Kazuo Nagasawa
CHEMICAL COMMUNICATIONS
(2020)