Review
Biochemistry & Molecular Biology
Ramesh Katam, Chuwei Lin, Kirstie Grant, Chaquayla S. Katam, Sixue Chen
Summary: This article reviews the recent technological advancements in metabolomics and their applications in understanding plant stress biology and different levels of spatial organization. Metabolomics plays a crucial role in bridging the gap between genotypes and phenotypes, however, the complexity of metabolites limits its advances. The applications of LC/GC-MS, NMR, and IMS-MS have greatly improved the identification and quantification of metabolites. Metabolites change in response to environmental conditions, and their spatial distribution is a trending research area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Leicheng Zhang, Tengfei Xu, Jingtao Zhang, Stephen Choong Chee Wong, Mark Ritchie, Han Wei Hou, Yulan Wang
Summary: The study proposes a novel strategy integrating spiral inertial microfluidics and ion mobility mass spectrometry for single-cell metabolite detection and identification. The technology successfully screened the metabolic profiles of different types of cancer cells, improving confidence in metabolite identification. This approach offers a simple and efficient method for single-cell lipid profiling, with additional ion mobility separation significantly enhancing the confidence toward identification of metabolites.
ANALYTICAL CHEMISTRY
(2021)
Review
Oncology
Zhiqiang Zhang, Chaohui Bao, Lu Jiang, Shan Wang, Kankan Wang, Chang Lu, Hai Fang
Summary: Resistance to drug treatment is a major problem in cancer therapy, and there is a need to explore cancer hallmarks that can be targeted to overcome this. Metabolic reprogramming has been identified as one such hallmark, and with metabolomics technology, targeting metabolic alterations in cancer cells is becoming a promising therapeutic strategy. This review discusses the latest progress in bulk, single-cell, and spatial metabolomics technologies and their applications in understanding cancer drug resistance.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
David Shorthouse, Jenna Bradley, Susan E. Critchlow, Claus Bendtsen, Benjamin A. Hall
Summary: The understanding of cellular metabolism in cancer is still in its early stages. Genetic alterations associated with cancer may lead to changes in cellular metabolism, which can provide insight into phenotypic changes, identify detectable metabolic patterns, and reveal vulnerabilities to specific drugs. By analyzing the metabolites of 173 different cancer cell lines from 11 tissues, we were able to correlate known cancer-associated mutations and gene expression programs with metabolic signatures, establishing new connections between known metabolic pathways and cancer driver genes. We also discovered that metabolic activity is correlated with drug sensitivity, allowing us to predict drug response and synergy based on metabolic activity. Additionally, we investigated the metabolic heterogeneity of cancer mutations across tissues and found a range of context-specific and more general metabolic control in genes.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Review
Spectroscopy
Rui Hu, Ying Li, Yunhuang Yang, Maili Liu
Summary: Single cell analysis is of increasing interest to the research community due to its ability to analyze cellular heterogeneity and discover new biomarkers. With advancements in technology, it is now possible to perform multiple omics at the single cell level. However, single-cell metabolomics poses unique challenges due to complex compound composition and the inability to amplify metabolites. Despite these challenges, significant progress has been made in mass spectrometry-based single-cell metabolomics.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Medicine, Research & Experimental
Abdulrahman L. Al-Malki, Ashraf Bakkar, Etimad A. Huwait, Elie K. Barbour, Kalid O. Abulnaja, Taha A. Kumosani, Said S. Moselhy
Summary: This study evaluated the anti-proliferative and pro-apoptotic effects of strigol/albumin/chitosan nanoparticles (S/A/CNP) on HepG2 cell line, finding that it can modulate energetic and epigenetic metabolites, making it a potential therapeutic agent for hepatocellular carcinoma.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Food Science & Technology
Xin Pang, Zitao Guo, Long Ao, Yuliang Yang, Chunyu Liu, Zhenghua Gu, Yu Xin, Moying Li, Liang Zhang
Summary: In this study, cell metabolomics and serum metabolomics were used to explore the potential mechanism of Inonotus hispidus in the treatment of hyperuricemia. The results suggest that Inonotus hispidus may show efficacy by regulating the synthesis of uric acid and lipid disturbances caused by hyperuricemia. This research elucidated potential biomarkers and mechanisms of action in the hypouricemic effect of Inonotus hispidus from the perspective of endogenous metabolites, providing a scientific basis for its further development and application.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Chemistry, Analytical
Shaojie Qin, Daiyu Miao, Xue Zhang, Yi Zhang, Yu Bai
Summary: Metabolomics at the single-cell level using mass spectrometry has great importance in revealing phenotypic heterogeneity and contributing to precision diagnosis of diseases. This review categorizes and summarizes the developments of mass spectrometry-based single-cell metabolomics according to different ionization methods, systematically introducing key parameters such as cell throughput, metabolite coverage, and imaging capability. Challenges and perspectives for further development of techniques, data integration, and biological applications in mass spectrometry-based single-cell metabolomics are proposed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jinhui Zhao, Xinshu Zhao, Jiaying Yu, Siqi Gao, Mingjia Zhang, Tongshu Yang, Liyan Liu
Summary: This study identified potential biomarkers for early screening of esophageal squamous cell carcinoma (ESCC) and characterized the underlying metabolic disturbances using multi-platform metabolomics analysis. A diagnostic panel consisting of five metabolites showed high accuracy in distinguishing ESCC patients from healthy controls.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biochemical Research Methods
Congrou Zhang, Sylvia E. Le Devedec, Ahmed Ali, Thomas Hankemeier
Summary: Single-cell metabolomics (SCMs) is a powerful tool for studying cellular heterogeneity by providing insight into the differences between individual cells. With the development of a set of promising SCMs pipelines, this maturing technology is expected to be widely used in biomedical research. However, before SCMs is ready for primetime, there are some challenges to overcome. In this review, we summarize the trends and challenges in the development of SCMs and highlight the latest methodologies, applications, and integration perspectives with other omics and imaging approaches.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Raphaela Fritsche-Guenther, Yoann Gloaguen, Alina Eisenberger, Jennifer A. Kirwan
Summary: This study optimized the detection and analysis of metabolites in adherent cell lysates using the AbsoluteIDQ p400 HR kit. The kit, validated for human plasma and serum, can also be used for relative quantification analysis of cell lysates from adherent cells.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Jian Ji, Jiadi Sun, Yinzhi Zhang, Xiulan Sun
Summary: This study used a metabolomics approach to investigate the effects of gold nanorods (GNRs) on human hepatoma cells. The shape, dose, and exposure time of GNRs were found to affect their cytotoxicity, with short GNRs causing disruption of cell metabolism. High concentrations of GNRs resulted in more significant toxicity. The main cytotoxicity of GNRs was attributed to mitochondrial toxicity, which disrupted the metabolism of several amino acids. However, the efflux function of cells allowed for the extracellular exclusion of GNRs within 24 hours, reducing mitochondrial metabolic toxicity and allowing for recovery of metabolic disorders.
Article
Biochemical Research Methods
Jianhua Cao, Xingkang Wu, Xuemei Qin, Zhenyu Li
Summary: Increasing cell passage number negatively affects HT29 cells, leading to decreased proliferation and migration ability, as well as distinct metabolomic differences among cells with different passage numbers, with arginine playing a key role in two key metabolic pathways, and exhibiting a synergistic effect with cisplatin.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Michael J. Taylor, Andrey Liyu, Akos Vertes, Christopher R. Anderton
Summary: LAESI-MS is an emerging method with the potential to revolutionize metabolomics, offering the advantages of minimal sample preparation and in situ analysis with high spatial resolution for cells and tissues.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Engineering, Environmental
Xiaoyao Song, Jianhui Liu, Ningbo Geng, Yichu Shan, Baoqin Zhang, Baofeng Zhao, Yuwen Ni, Zhen Liang, Jiping Chen, Lihua Zhang, Yukui Zhang
Summary: This study integrated cytotoxicity, component profiling, metabolomics, and proteomics data to understand the mechanisms of PM2.5-induced cytotoxicity in human bronchial epithelial cells. PM2.5 was found to cause cell cycle arrest, calcium influx, cell damage, and apoptosis, with heavy metals and PAHs in PM2.5 correlating with intracellular ROS levels. Metabolomics and proteomics analysis revealed significant alterations in metabolic processes, while PM2.5 was shown to inhibit the integrin signaling pathway, affecting cell cycle progression, metabolism, and apoptosis.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Dan-na Huang, Fang-fang Wu, Ai-hua Zhang, Hui Sun, Xi-jun Wang
Summary: The study recaps the potential therapeutic effects and mechanisms of action of berberine in treating rheumatoid arthritis, as well as discusses its modulation of gut microbiota during RA. Berberine may be a promising lead drug with multi-functional activities for the therapeutic strategy of RA.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Taiping Li, Mengli Zhang, Zhien Tan, Jianhua Miao, Yanmei He, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: A rapid and robust method was established for the comprehensive analysis of the chemical composition of Jigucao capsule for the first time in this study, providing important reference for the quality control and pharmacodynamic material basis research of Jigucao capsule.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Hui-Yu Li, Hui Sun, Ai-Hua Zhang, Lu-Wen He, Shi Qiu, Jun-Ru Xue, Fangfang Wu, Xi-Jun Wang
Summary: Through urine metabolomics analysis, SMYAT was found to have significant antithrombotic and anti-inflammatory effects on thromboangiitis obliterans. It can regulate the metabolic profile of TAO rats, and the therapeutic effect is closely related to tyrosine metabolism, vitamin B6 metabolism, and cysteine and methionine metabolism.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yanmei He, Mengli Zhang, Taiping Li, Zhien Tan, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: This study investigated the mechanism of Jigucao capsule (JGCC) in the treatment of Dampness-heat Jaundice Syndrome (DHJS) using urine metabolomics. The results showed that JGCC significantly improved clinical biochemical indexes and histopathological changes, and adjusted disturbed biomarkers, indicating its remarkable effect on the treatment of DHJS.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Oncology
Jing Li, Ai-hua Zhang, Fang-fang Wu, Xi-jun Wang
Summary: Dysbiosis of gut bacteria is associated with colorectal cancer (CRC), and the association analysis of metabolomics and gut microbiome will provide novel strategies for the prevention, diagnosis, and therapy of CRC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Microbiology
Changhong Ding, Dan Yu, Yiyang Chen, Junyi Zhu, Junkai Wu, Xiaowei Du, Xijun Wang
Summary: This study sequenced and analyzed the genome of endophytic fungus Fusarium sp. C39 and found key enzyme genes involved in saponin synthesis. The proposed mechanism of saponin production in Fusarium sp. C39 provides a theoretical basis for efficient and low-cost production of saponins.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Taiping Li, Yanmei He, Mengli Zhang, Zhien Tan, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: This study explored the potential mechanism of the Chinese patent medicine Jigucao capsule in treating the serum metabolic profile of rats with Yanghuang syndrome. The results showed that Jigucao capsule reduced inflammatory cells, inhibited the proliferation of bile duct epithelial cells and hepatocyte necrosis. It also significantly reversed abnormal levels of certain metabolites related to lipid and carbohydrate metabolism. In conclusion, Jigucao capsule has a therapeutic effect on rats with Yanghuang syndrome, and this study provides basic data for further pharmacodynamic studies.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Xing-Hua Li, Feng-Ting Yin, Xiao-Hang Zhou, Ai-Hua Zhang, Hui Sun, Guang-Li Yan, Xi-Jun Wang
Summary: This study summarizes the key pathways and targets of natural compounds from traditional Chinese medicine in the treatment of ischemic stroke by reviewing relevant literature over the past five years. This provides a valuable information base for the future development of effective treatments for ischemic stroke.
Article
Biochemistry & Molecular Biology
Qingyu Zhang, Aihua Zhang, Fangfang Wu, Xijun Wang
Summary: This study used metabonomics technology to reveal the regulatory effect of Yunnan Baiyao on small molecular metabolites in promoting blood circulation and removing blood stasis, and identified 28 urine biomarkers. The results demonstrated that Yunnan Baiyao improved the pathological indexes in rats with blood stasis syndrome and clarified the relevant metabolic pathways involved in its treatment.
Article
Chemistry, Analytical
Nannan Huang, Dan Yu, Jinhai Huo, Junkai Wu, Yiyang Chen, Xiaowei Du, Xijun Wang
Summary: This study conducted solid fermentation of Dioscorea nipponica using endophytic fungi C39 and found that it could effectively transform the saponin components, leading to a significant increase in diosgenin concentration. Furthermore, 11 new metabolites were identified during the biotransformation process of D. nipponica, and the transformation pathways were inferred based on the variation trend of saponins.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Wen-feng Wei, Hui Sun, Shao-bo Liu, Sheng-wen Lu, Ai-hua Zhang, Wan-ying Wang, Wen-jun Chai, Fang-fang Wu, Guang-li Yan, Yu Guan, Xi-jun Wang
Summary: By studying animal models and utilizing metabonomics tools, the effective components of ZhiziBaipi Decoction in treating Damp-heat jaundice syndrome and their correlation with biomarkers were identified, providing new insights into its therapeutic mechanism.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Taiping Li, Fangfang Wu, Aihua Zhang, Hui Dong, Ihsan Ullah, Hao Lin, Jianhua Miao, Hui Sun, Ying Han, Yanmei He, Xijun Wang
Summary: By combining untargeted metabolomics and serum pharmacochemistry of traditional Chinese medicine, this study revealed the metabolic mechanisms and key pharmacodynamic components of Wutou decoction in rheumatoid arthritis rats, providing a theoretical basis for quality control investigation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ling Kong, Ye Sun, Hui Sun, Ai-hua Zhang, Bo Zhang, Nan Ge, Xi-jun Wang
Summary: This study applied the chinmedomics strategy to discover the effective constituents of KLX in the treatment of diabetic retinopathy (DR), and revealed some of the mechanisms involved.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Endocrinology & Metabolism
Ye Sun, Ling Kong, Ai-Hua Zhang, Ying Han, Hui Sun, Guang-Li Yan, Xi-Jun Wang
Summary: Diabetic retinopathy, a serious complication of diabetes, is a leading cause of blindness worldwide. The irreversibility of the condition makes current treatment methods unsatisfactory. Early detection and intervention are important in reducing the damage caused by diabetic retinopathy. Metabolomics, a branch of systems biology, can identify characteristic metabolic changes associated with the progression of diabetic retinopathy and discover potential biomarkers for clinical diagnosis and treatment. This review summarizes the known metabolomics biomarkers of diabetic retinopathy and discusses the possibility of the arginine-creatine metabolic pathway as a therapeutic strategy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Feng-ting Yin, Xiao-hang Zhou, Shu-yu Kang, Xing-hua Li, Jing Li, Ihsan Ullah, Ai-hua Zhang, Hui Sun, Xi-jun Wang
Summary: This study explored the main constituents and potential targets of Dachengqi Decoction (DCQ) in the treatment of colorectal cancer (CRC) using serum pharmacochemistry and network pharmacology. The results showed that flavonoids and anthraquinones were the main active substances of DCQ, which may exert anti-tumor effects by regulating targets such as MAPK, IL-6, and VEGF, as well as pathways including PI3K-AKT signaling pathway, AGE-RAGE signaling pathway, and IL-17 signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)