Article
Agriculture, Dairy & Animal Science
Shifeng Pan, Lin Zhang, Zhuang Liu, Hua Xing
Summary: The study revealed that MSTN can inhibit lipid accumulation and promote lipolysis in porcine subcutaneous preadipocytes, mainly through activating the crosstalk between ERK1/2 and PKA signaling pathways.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Xiaocheng Huang, Chenchen Bian, Hong Ji, Shanghong Ji, Jian Sun
Summary: This study investigated the effects of docosahexaenoic acid (DHA) on lipid metabolism in grass carp. It was found that DHA promotes lipolysis by triggering endoplasmic reticulum stress and activating the cAMP/PKA signaling pathway. These findings provide new insights into the molecular mechanisms of DHA-induced lipolysis and its potential as a nutritional strategy for reducing lipid accumulation in freshwater fish.
Article
Biochemistry & Molecular Biology
Xiong Wang, Fan Liu, Qin An, Wenli Wang, Zhimei Cheng, Yunping Dai, Qingyong Meng, Yali Zhang
Summary: Lactoferrin (Ltf) plays a protective role in skeletal muscle by promoting the proliferation of satellite cells (SCs) and skeletal muscle regeneration. It holds promise as a novel therapeutic agent for skeletal muscle injuries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ranran Wang, Lulu Wei, Junaid Wazir, Li Li, Shiyu Song, Kai Lin, Wenyuan Pu, Chen Zhao, Zhonglan Su, Quan Zhao, Hongwei Wang
Summary: Curcumin improves adipose atrophy in cancer cachexia by inhibiting the cAMP/PKA/CREB signaling pathway. The study shows that curcumin protects mice with C26 tumors and cachexia from weight loss and adipose atrophy, reducing levels of free fatty acids and increasing triglyceride levels. Curcumin also ameliorates CL316243-induced adipose atrophy and inhibits hormone-mediated PKA and CREB activation.
Article
Biochemistry & Molecular Biology
Jang Mi Han, Hong Lae Kim, Hye Jin Jung
Summary: Ampelopsin (dihydromyricetin), a plant-derived flavonoid, significantly inhibits the proliferation of two leukemia cell lines while not affecting the viability of normal cells. Its anticancer effects include cell cycle regulation, induction of apoptosis, and downregulation of signaling pathways. Ampelopsin shows promise as an attractive chemotherapeutic agent against leukemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Emilia Przygrodzka, Xiaoying Hou, Pan Zhang, Michele R. Plewes, Rodrigo Franco, John S. Davis
Summary: Luteinizing hormone (LH) regulates ovulation and formation of the corpus luteum, while AMP-activated protein kinase (AMPK) exerts inhibitory effects on steroidogenesis in luteal cells by modulating the actions of PKA. AMPK regulates cholesterol availability for steroidogenesis by mediating phosphorylation of hormone-sensitive lipase (HSL), in contrast to the phosphorylation effects of LH/PKA on HSL.
Article
Medicine, Research & Experimental
Yunmei Zhang, Yuan Zhang, Zizhang Wang, Yufang Sun, Xinghong Jiang, Man Xue, Yafeng Yu, Jin Tao
Summary: This study reveals that gentamicin decreases the I-DR in VG neurons through JNK and PKA-mediated signaling pathways, leading to neuronal hyperexcitability. Blocking JNK signaling and I-DR can attenuate this effect.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Virology
Shilin Gong, Xianhui Hao, Yanhong Bi, Chenchen Yang, Wenjing Wang, Houfack K. Mickael, Yike Zhang, Shuangfeng Chen, Zhongyao Qian, Fen Huang, Daqiao Wei, Wenhai Yu
Summary: HEV infection significantly inhibits the cAMP-PKA-CREB and PI3K-AKT-mTOR signaling pathways, but not the Ras-Raf-MEK-ERK pathway. Elevated estrogen levels and highly activated ER alpha during pregnancy exacerbate HEV replication. This exacerbation further inhibits ER alpha expression and suppresses cAMP-PKA-CREB and PI3K-AKT-mTOR signaling pathways.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Han Tian, Yanni Gui, Yuanhui Wei, Bin Shang, Jian Sun, Shan Ma, Wenjie You, Shujuan Jiang
Summary: Z-GS has been shown to have anti-tumor effects in NSCLC cells and mouse LLC tumors, while also increasing PD-L1 expression levels through inhibition of FXR and activation of Akt and Erk1/2 signaling pathways. These findings suggest potential for synergistic combined therapies in NSCLC.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Respiratory System
Rui-Gang Zhang, Ya Niu, Ke-Wu Pan, Hao Pang, Chun-Ling Chen, Chung-Yin Yip, Wing-Hung Ko
Summary: The activation of beta(2)-adrenoceptors in human bronchial epithelial cells stimulates specific signaling pathways, leading to the release of pro-inflammatory cytokine IL-6, but not IL-8. The pathways involving PKA/Src/ERK1/2 and beta-arrestin2 play essential roles in this process.
Article
Biochemistry & Molecular Biology
Hyo-Shim Han, Ilavenil Soundharrajan, Mariadhas Valan Arasu, Dahye Kim, Ki-Choon Choi
Summary: This study investigated the effects of Leuconostoc citreum (LSC) cell-free metabolites on adipogenesis, lipogenesis, and lipolysis in 3T3-L1 adipocytes. The results showed that LSC treatment reduced lipid droplet accumulation and the expression of key adipogenic and lipogenic markers while increasing lipolysis. Additionally, LSC treatment elevated adiponectin levels, suggesting its potential as a novel probiotic strain for treating obesity and metabolic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zeinab Babaei, Mojtaba Panjehpour, Seyedeh Sara Ghorbanhosseini, Hadi Parsian, Mahsa Khademi, Mahmoud Aghaei
Summary: This study demonstrated the signaling pathways of VEGFR3 in ovarian cancer cells through upregulated expression of miR-1236. Inhibition of VEGFR3 using miR-1236 reduced cell proliferation, migration, and invasion ability, and increased apoptosis rate in SKOV3 and OVCAR3 cells. Additionally, VEGFR3 stimulation increased ERK1/2 and AKT phosphorylation, while VEGFR3 suppression resulted in decreased phosphorylation of ERK1/2 and AKT. These findings suggest that the miR-1236/VEGFR3 axis regulates ovarian cancer cell proliferation by modulating ERK1/2 and AKT signaling, providing a potential therapeutic strategy against ovarian cancer.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xiao Liu, Yunhe Fu, Jingjing Wang, Di Wu, Shuangqiu Li, Chaoqun Wang, Zhengtao Yang, Ershun Zhou
Summary: Beta-conglycinin is a key thermostable anti-nutritional factor in soybean that can induce allergic reactions in young animals. This study found that beta-conglycinin can induce the formation of neutrophil extracellular traps (NETs) in a manner dependent on ROS-derived NADPH oxidase, ERK1/2, p38, Rac, and PAD4 signaling pathways, shedding light on the underlying mechanisms and potential clinical applications.
Article
Biology
Lili Fan, Zhenqiang Li, Linlin Gao, Nan Zhang, Wenxiao Chang
Summary: Chronic periodontitis is an inflammatory disease characterized by gum tissue inflammation. This study found that isoimperatorin reduced viability of human periodontal membrane fibroblasts and alleviated oxidative stress in these cells. Isoimperatorin also reduced proinflammatory factor secretion and osteoclast differentiation in periodontal membrane cells. Furthermore, isoimperatorin inhibited the activation of ERK1/2 and nuclear factor-kappa B pathways. Isoimperatorin could therefore be a promising drug for the treatment of chronic periodontitis.
OPEN LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Stephanie S. Kim, Ina Kycia, Michael Karski, Rosanna K. Ma, Evan A. Bordt, Julian Kwan, Anju Karki, Elle Winter, Ranan G. Aktas, Yuxuan Wu, Andrew Emili, Daniel E. Bauer, Praveen Sethupathy, Khashayar Vakili
Summary: Fibrolamellar carcinoma (FLC) is a primary liver cancer with a poor prognosis. The study engineered the DP fusion gene in HEK293T cells to investigate its cellular effects. The results showed alterations in mitochondrial dynamics and increased LINC00473 expression in HEK-DP cells, suggesting potential roles of DP in mitochondrial dynamics and LINC00473 activity.
Article
Plant Sciences
Ichen Tsai, Chia-Lin Chung, Shiou-Ruei Lin, Ting-Hsuan Hung, Tang-Long Shen, Chih-Yi Hu, Wael N. Hozzein, Hiran A. Ariyawansa
Summary: This study identified and characterized the causative agents of tea gray blight disease in Taiwan, revealing seven well-classified taxa and seven tentative clades within three genera, including one novel species and five newly recorded species. Pathogenicity assessments showed that all assayed isolates were pathogenic on tea leaves, with Pseudopestalotiopsis chinensis and Pseudopestalotiopsis camelliae-sinensis identified as the major pathogens associated with gray blight disease of tea in Taiwan. This study is the first to focus on the diversity, pathogenicity, and characterization of pestalotiopsis-like fungi causing tea gray blight disease in Taiwan.
Article
Biotechnology & Applied Microbiology
Yu-Chen Cheng, Shih-Chung Chang
Summary: The study prepared two monoclonal antibodies, 7H6C and YC8, with subtype specificity for H5N8 and H5Nx avian influenza viruses, which can be used for specific detection of HA proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jui-Chung Chiang, Wei-Min Chen, Kuan-Hung Lin, Kai Hsia, Ya-Hsuan Ho, Yueh-Chien Lin, Tang-Long Shen, Jen-Her Lu, Shih-Kuo Chen, Chao-Ling Yao, Benjamin P. C. Chen, Hsinyu Lee
Summary: This study reveals that lysophosphatidic acid (LPA) has opposing regulatory effects on erythrocyte differentiation through activation of LPA receptors 2 and 3. The expression of LPA(2) and LPA(3) in murine myeloid cells may determine the direction of myeloid differentiation, with LPA(2) inhibiting early myeloid differentiation and LPA(3) promoting erythrocyte production. Activation of LPA(3) is essential for the restoration of acute hemolytic anemia and correlates with erythropoiesis impairment in a premature aging model.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Chemistry, Applied
Bao-Hong Lee, She-Ching Wu, Tang-Long Shen, Yi-Yun Hsu, Chia-Hsiu Chen, Wei-Hsuan Hsu
Summary: The study showed that extracellular vesicles (EVs) isolated from Lactobacillus plantarum can effectively inhibit oxidation reactions and bacteria growth in tuna fish during storage, indicating their potential application in food storage technology.
Article
Biotechnology & Applied Microbiology
Yi-Wei Chiang, Chia-Jung Li, Heng-Yi Su, Kai-Ting Hsieh, Chia-Wei Weng, Hui-Wen Chen, Shih-Chung Chang
Summary: The avian influenza A(H7N9) virus has caused numerous cases of human infection since 2013, with a high mortality rate. Monoclonal antibodies F3-2 and 1C6B were successfully generated to specifically detect H7N9 HA, which can be utilized for the detection of H7N9 virus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tzu-Heng Wu, Ching-Hsu Yang, Chia-Chen Chang, Hui-Wen Liu, Chia-Yu Yang, Tang-Long Shen, Chii-Wann Lin, Aurelien Bruyant
Summary: Phase-sensitive surface plasmon resonance biosensors are highly sensitive, but measuring the phase sensorgram at a fixed angle set-up may result in low reproducibility. One potential solution is to map the phase sensorgram into refractive index unit using sensor calibration data. However, basic fitting functions poorly portray the asymmetric phase curve.
Article
Biotechnology & Applied Microbiology
Chia-Jung Li, Ping-Han Huang, Hui-Wen Chen, Shih-Chung Chang
Summary: The study developed four Zika virus envelope protein-specific monoclonal antibodies, with mAb 9E-1 showing the strongest affinity to ZIKV E protein. However, mAbs A1, C1, and 9E-1 do not cross-react with dengue virus, and mAbs B1 and 9E-1 have been developed for specific detection of ZIKV E protein and virions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Siou-Ru Shen, Wei-Jen Chen, Hui-Fang Chu, Shiuan-Huei Wu, Yu-Ru Wang, Tang-Long Shen
Summary: Studies have shown that S. thermophilus ST4 derived from raw milk can effectively improve 5-FU-induced intestinal mucositis, including alleviating symptoms such as weight loss, loss of appetite, and diarrhea, reducing intestinal inflammation, and increasing acetic acid expression, demonstrating good mucoprotective effects.
Article
Biochemistry & Molecular Biology
Li-Chun Chang, Han-Mo Chiu, Ming-Shiang Wu, Tang-Long Shen
Summary: Colorectal cancer (CRC) is a common cancer worldwide, and there is a need for more acceptable screening methods. Blood tests have been suggested as a first-line approach, and the identification of blood biomarkers for CRC detection is urgently needed. Recent studies have shown that small extracellular vesicles (sEVs) are effective in detecting CRC, but there is limited research on their diagnostic performance in clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Guan-Chun Lai, Tai-Ling Chao, Shiau-Yu Lin, Han-Chieh Kao, Ya-Min Tsai, De-Chao Lu, Yi-Wei Chiang, Sui-Yuan Chang, Shih-Chung Chang
Summary: Two mouse monoclonal antibodies, 7 Eb-4G and 1Ba-3H, were identified to specifically recognize the receptor-binding domain (RBD) of SARS-CoV-2 spike protein. Only 1Ba-3H exhibited neutralizing activity and interfered with the binding between RBD and ACE2. Epitope mapping experiments revealed specific residues essential for 1Ba-3H binding. Interestingly, 1Ba-3H showed neutralizing activity against several SARS-CoV-2 variants but enhanced cellular infection of the Gamma variant.
ANTIVIRAL RESEARCH
(2022)
Article
Microbiology
Chia-Jung Li, Tai-Ling Chao, Ting-Yu Chang, Chia-Chun Hsiao, De-Chao Lu, Yi-Wei Chiang, Guan-Chun Lai, Ya-Min Tsai, Jun-Tung Fang, Siman Ieong, Jann-Tay Wang, Sui-Yuan Chang, Shih-Chung Chang
Summary: This study identified four S2-specific neutralizing antibodies (nAbs) that inhibit SARS-CoV-2 infection by blocking S protein-mediated membrane fusion. These nAbs exhibited broad neutralizing activity against different variants of SARS-CoV-2. The discovery of these nAbs and their unique antigenic epitopes provides new opportunities for the development of COVID-19 therapeutics and vaccines.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Dairy & Animal Science
Fang-Yu Lai, Yu-Chen Lin, Shih-Torng Ding, Chi-Sheng Chang, Wi-Lin Chao, Pei-Hwa Wang
Summary: This study isolated novel microsatellite markers for monitoring the genetic structure of domestic dog populations in Taiwan, providing a powerful tool for genetic monitoring.
Article
Biochemistry & Molecular Biology
Anjali Yadav, Thamil Selvee Ramasamy, Sheng-Che Lin, Szu-Han Chen, Jean Lu, Ya-Hsin Liu, Fu- Lu, Yuan-Yu Hsueh, Shau-Ping Lin, Chia-Ching Wu
Summary: The failure of peripheral nerve regeneration may be attributed to the lack of a permissive environment for nerve repair. The use of autologous platelet-rich plasma (PRP) or its derivative platelet-rich growth factors (PRGF) has been shown to improve peripheral nerve regeneration by modulating macrophage polarization. In this study, PRGF was found to reduce inflammation and promote the shift of macrophages from a pro-inflammatory M1 phenotype to an anti-inflammatory M2 phenotype. This led to improved nerve regeneration in a rat sciatic nerve transection model. The findings suggest that autologous PRGF has the potential to regulate inflammatory microenvironments and promote nerve repair.
Article
Agriculture, Dairy & Animal Science
Fang-Yu Lai, Chung-Ying Yin, Shih-Torng Ding, Po-An Tu, Pei-Hwa Wang
Summary: This study analyzed the genetic structure of Taiwan black (TB) goats using 15 microsatellite markers, and successfully clustered the goat populations in Taiwan. The results contribute to the preservation and utilization of TB goats.
ANIMAL BIOTECHNOLOGY
(2022)
Review
Immunology
Chia-Jung Li, Shih-Chung Chang
Summary: Since the onset of COVID-19, numerous NAbs against SARS-CoV-2 have been developed, but the emergence of immune escape variants threatens their effectiveness. However, NAbs targeting the S2 subunit of the S protein may offer broader neutralizing potency. This review discusses the binding specificity and functional features of S2-specific NAbs, providing insights for antibody cocktail therapy and next-generation vaccines.
EMERGING MICROBES & INFECTIONS
(2023)