Article
Biochemistry & Molecular Biology
Loretta Ferrera, Raffaella Barbieri, Cristiana Picco, Paolo Zuccolini, Alessia Remigante, Sara Bertelli, Maria Rita Fumagalli, Giovanni Zifarelli, Caterina A. M. La Porta, Paola Gavazzo, Michael Pusch
Summary: The response to oxidative stimulation in melanoma cells involves the activation of potassium currents and an increase in intracellular calcium levels, mediated by BK, KCa3.1, and TRPM2 channels. Differences in the expression levels of these channels in different cell lines may play a crucial role in melanoma progression. Targeting these ion channels pharmacologically could be a novel strategy to reduce the metastatic potential of melanoma cells alongside traditional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Akito Nakao, Yoshihiro Matsunaga, Katsumi Hayashida, Nobuaki Takahashi
Summary: Psychiatric disorders are believed to be caused by complex and diverse factors, with oxidative stress playing a crucial role in the pathogenesis of major disorders such as bipolar disorder, depression, anxiety disorder, and schizophrenia. Calcium channels are essential for neuronal functions, and the investigation of redox-sensitive TRP channels could lead to the development of new therapeutic strategies for psychiatric disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Viviane Nascimento Da Conceicao, Yuyang Sun, Karthik Ramachandran, Arun Chauhan, Amritha Raveendran, Manigandan Venkatesan, Bony DeKumar, Soumya Maity, Neelanjan Vishnu, George A. Kotsakis, Paul F. Worley, Donald L. Gill, Bibhuti B. Mishra, Muniswamy Madesh, Brij B. Singh
Summary: The transformation of naive macrophages into M1 or M2 phenotypes is regulated by distinct Ca2+ entry channels. Blocking Ca2+ entry can suppress signaling events related to cytokine production and reduce the inflammatory response of macrophages, depending on the expression levels of Orai1 and TRPC1 channels.
Review
Physiology
Francesco Moccia, Alessandra Fiorio Pla, Dmitry Lim, Francesco Lodola, Andrea Gerbino
Summary: Cytosolic Ca2+ signals play a vital role in regulating cellular functions, and they are controlled by a complex interplay of various molecular mechanisms. Recent research has uncovered unexpected roles of Ca2+ signaling in kidney water reabsorption, Alzheimer's disease-related astroglial dysfunction, cancer hallmarks, and cardiovascular system function. These findings have expanded our understanding of Ca2+ signaling and its implications in health and disease.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Maurice B. Hallett
Summary: Phagocytosis is a highly polarised cellular activity where the stimulus and response are focused at one specific part of the cell. The mechanisms for restricting signalling and achieving effective phagocytosis are still not fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Madelaine Magali Audero, Natalia Prevarskaya, Alessandra Fiorio Pla
Summary: This review presents the impact of hypoxia and acidosis in the tumor microenvironment (TME) on cancer progression, and focuses on the role of Ca2+ channels in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisandra Flores-Aldama, Daniel Bustos, Deny Cabezas-Bratesco, Wendy Gonzalez, Sebastian E. Brauchi
Summary: TRPV5 and TRPV6 are calcium-selective ion channels that play a crucial role in maintaining systemic calcium homeostasis. These channels are regulated by intracellular calcium, which induces inactivation. The inactivation of TRPV5 and TRPV6 can be divided into fast and slow phases, with the fast inactivation being unique to TRPV6. By utilizing various techniques, including structural analyses and mutagenesis, we have identified specific amino acids and interactions that govern the inactivation kinetics of mammalian TRPV5 and TRPV6 channels. Additionally, we propose that the association between the intracellular HLH domain and the TRP domain helix contributes to the faster inactivation kinetics observed in mammalian TRPV6 channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Eva Schmidt, Chamali Narangoda, Wolfgang Norenberg, Miyuki Egawa, Anna Rossig, Marion Leonhardt, Michael Schaefer, Susanna Zierler, Maria G. Kurnikova, Thomas Gudermann, Vladimir Chubanov
Summary: The study identifies a regulatory site in the TRPM7 channel where intracellular Mg2+ binds to control the channel activity. This reveals a new structure-function relationship in TRPM7 channels.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Carmen Costas-Ferreira, Lilian R. F. Faro
Summary: Pesticides from different chemical classes exert toxic effects on the nervous system by altering calcium homeostasis, leading to oxidative stress, neuroinflammation, and ultimately neuronal death. Proposed strategies to counteract these effects are also discussed in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Katharina E. M. Hellenthal, Laura Brabenec, Eric R. Gross, Nana-Maria Wagner
Summary: The transient receptor potential (TRP) cation channel superfamily consists of more than 50 channels playing crucial roles in physiological processes. Aldehydes can activate TRP channels, triggering a cellular cascade that may lead to disease pathophysiology. Studies have shown that aldehyde dehydrogenase 2 protects against aldehyde toxicity by clearing toxic aldehydes.
Article
Biology
Takahiro Takano, Amanda M. Wahl, Kai-Ting Huang, Takanori Narita, John Rugis, James Sneyd, David Yule
Summary: In this study, researchers found that the Ca2+ signals in salivary secretion process in live mice initiate in the apical endoplasmic reticulum, forming highly localized trains of transient that fail to fully propagate, leading to large apical-basal gradients following optimal stimuli for secretion. A new mathematical model incorporating these findings will help to understand how salivary secretion can be optimally stimulated by apical Ca2+ signals.
Review
Cell Biology
Sung-Min Hwang, Ji Yeon Lee, Chul-Kyu Park, Yong Ho Kim
Summary: Neurodegenerative diseases (NDs) primarily affect neurons and glial cells in the brain, with risk factors and pathophysiological mechanisms not fully understood. Homeostasis of intracellular Ca2+ concentration and pH is crucial for cell function, with TRP channels and PMCA playing vital roles in modulating ionic homeostasis. Dysfunctions of TRP channels and PMCA disrupt neuronal homeostasis under pathological conditions, resulting in various NDs. This review highlights their potential as novel targets for treating NDs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yaping Wang, Jian Shi, Xiaoyong Tong
Summary: Mechanosensitive ion channels play a crucial role in the cardiovascular system by translating mechanical forces into biological signals, particularly in regulating calcium ion channels involved in cardiovascular physiological activity. Moreover, their interactions with Ca2+ regulatory proteins are important for maintaining calcium homeostasis and cardiovascular health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Dorina Zoephel, Chantal Hof, Annette Lis
Summary: Aging is an unstoppable process that affects every cell of the organism, especially the changes in Ca2+ signaling in immune cells which may profoundly impact the development of age-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xinhe Yang, Shuai Chen, Shuo Zhang, Sai Shi, Rui Zong, Yiting Gao, Bingcai Guan, Nikita Gamper, Haixia Gao
Summary: Zinc serves as a cofactor for numerous enzymes, transcription factors, receptors, and other proteins. It acts as an important signaling molecule and can activate Kv7 channels, protecting neurons from overactivity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
D. Duzgun Ergun, S. Dursun, N. Pastaci Ozsobaci, M. Naziroglu, D. Ozcelik
Summary: This study elucidated the relationship between hypercapnia and the TRPM2 channel, as well as the effects of zinc, selenium, and glutathione. Experimental results showed that hypercapnia increased oxidative stress, but zinc, selenium, and glutathione were found to protect cells from damage caused by oxidative stress.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yusuf Cagdas Kumbul, Mustafa Naziroglu
Summary: This study investigates the effects of paclitaxel (PAX) and melatonin (MLT) on human laryngeal squamous cancer cells (Hep2) and the underlying mechanisms. The results show that PAX and MLT can induce cell death and apoptosis in Hep2 cells by affecting intracellular Ca2+ and Zn2+ levels. Furthermore, MLT treatment enhances the anticancer effects of PAX and reduces resistance to PAX. The findings also suggest that TRPM2 plays a critical role in this process.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Correction
Biochemistry & Molecular Biology
Zine Kechrid, Malika Hamdiken, Mustafa Naziroglu, Manuel Flores-Arce
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Endocrinology & Metabolism
Mustafa Naziroglu
Summary: Oxidative stress-induced activation of TRPM2 and TRPV4 channels can cause neuronal and cellular injury. This study discovered the potential antagonist properties of carvacrol (CARV) against TRPM2 and TRPV4 activations induced by oxidative stress, suggesting a protective role of CARV in neurodegenerative diseases associated with oxidative stress.
METABOLIC BRAIN DISEASE
(2022)
Article
Ophthalmology
Hatice Daldal, Mustafa Nazirogluz
Summary: This study investigated the modulatory effect of carvacrol on cell apoptosis and retinal oxidative damage induced by high blood glucose. The results showed that carvacrol alleviated cell death and oxidative stress caused by high blood glucose by inhibiting the activity of the TRPM2 channel.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Endocrinology & Metabolism
Ahmi Oz, Ramazan Cinar, Mustafa Naziroglu
Summary: This study investigated the cytosolic localization of TRPV1 and its relationship with oxidative neurotoxicity in glial cells. The results showed that TRPV1 was expressed in the perinuclear area of glial cells and not in the cell membrane. In DBTRG cells, capsaicin stimulation activated TRPV1 channels, leading to an increase in intracellular free reactive oxygen species and cell apoptosis. These effects were diminished by the treatment of a TRPV1 blocker.
METABOLIC BRAIN DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Bunyamin Aydin, Mustafa Naziroglu
Summary: This study investigated the effects of selenium and curcumin on STZ-induced neuropathic pain in mice by modulating TRPM7 activity. The results showed that STZ activation of TRPM7 led to neuropathic pain, oxidative neurotoxicity, and apoptosis in the mice. However, treatment with selenium and curcumin reduced these effects by downregulating TRPM7 activity.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yener Akyuva, Mustafa Nazirog
Summary: In this study, we investigated the effects of silver nanoparticles (AgNPs) and cisplatin (CiSP) exposure on glioblastoma cells through the activation of TRPM2 cation channels. We found that the cytotoxic and apoptotic actions of CiSP were enhanced by the treatment of AgNPs, which activated TRPM2. The oxidant actions of AgNPs were mediated by various cellular processes involving mitochondrial membrane depolarization, lipid peroxidation, and ROS generation. The combination of AgNPs and CiSP showed a synergistic action in the treatment of glioblastoma tumor cells through the stimulation of TRPM2.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Muge Mavioglu Kaya, Inan Kaya, Mustafa Naziroglu
Summary: The activation of TRPA1, TRPM2, and TRPV1 channels increases apoptotic and oxidant actions in colon cancer cells. Sambucus ebulus L (SEB) treatment inhibits the actions of these channels, thereby reducing colon tumor growth. Hence, TRPA1, TRPM2, and TRPV1 activations could be potential targets for colon tumor treatment.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pharmacology & Pharmacy
Kemal Ertilav, Mustafa Naziroglu
Summary: The study found that melittin (MLT) treatment can enhance the cytotoxic effects of cisplatin (CSP) on tumor cells by modulating Ca2+ channels. This enhancement is achieved through the activation of TRPM2 channels, which leads to the accumulation of mitochondrial reactive oxygen species (MitSOX) induced by mitochondrial Ca2+ and Zn2+. The study aimed to investigate the effects of CSP and MLT combination on DBTRG-05MG cells, including cell viability, cell death, apoptosis, and MitSOX levels.
Article
Parasitology
Mehmet Aykur, Cansu Caliskan Kurt, Derya Dirim Erdogan, Cigir Biray Avci, Rukiye Vardar, Sohret Aydemir, Nogay Girginkardesler, Cumhur Gunduz, Hande Dagci
Summary: This study examined the distribution and genetic diversity of Blastocystis sp. in stool samples from patients with gastrointestinal complaints in Izmir, Turkey. The prevalence of Blastocystis sp. was found to be 16.6%, with three different subtypes identified. Diarrhea was the most prominent clinical symptom in Blastocystis sp. positive patients.
ACTA PARASITOLOGICA
(2023)
Article
Neurosciences
Kenan Yildizhan, Mustafa Naziroglu
Summary: TRPM2 channel is activated by excessive generation of mitochondrial and cytosolic ROS and influx of intracellular free Ca2+. The inhibitions of NMDA receptor and TRPM2 channel decrease ROS and apoptosis in neurons. The modulator role of memantine and NMDA via modulation of TRPM2 on oxidative neurodegeneration and apoptosis in SH-SY5Y cells was investigated. Inhibition of NMDA receptor via memantine treatment modulated HYP-mediated ROS, apoptosis, and TRPM2-induced excessive Ca2+ and may provide a strategy for protecting HYP-mediated neurodegenerative diseases.
Article
Obstetrics & Gynecology
Yusuf Dal, Mustafa Naziroglu, Mehmet Okan Ozkaya
Summary: This study aimed to investigate the effects of low molecular weight heparin (LMWH) on calcium signaling, oxidative toxicity, and apoptosis in the thrombocytes of recurrent pregnancy loss (RPL) patients via modulation of TRPM2 and TRPV1 channels. The results showed that the concentrations of [Ca2+] (i), cytROS, mitochondrial membrane potential, apoptosis, caspase-3, and caspase-9 were high in the plasma and thrombocytes of RPL patients, but were diminished by the treatments of LMWH, TRPM2, and TRPV1 channel blockers.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2023)
Article
Parasitology
Mehmet Aykur, Hande Dagci
Summary: In this study, soil samples were collected from Izmir, Turkey, and a qPCR assay was used to determine the quantitative concentration of pathogenic free-living amoeba (Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri) in the soil. The results showed that these organisms were present in different soil samples at high concentrations. Therefore, people should be aware of the potential infection risks associated with contact with soil, especially in garden and potting soil samples.
Article
Pharmacology & Pharmacy
Mehmet Aykur, Nazan Goksen Tosun, Ozlem Kaplan, Aykut Ozgur
Summary: Acanthamoeba is a common protozoan that can cause eye infections and granulomatous amoebic encephalitis. There is an urgent need to develop new treatments for Acanthamoeba. Green nanotechnology synthesis approaches, such as using the crude extract of Allium tuncelianum, may be a good strategy. This study used microwave-assisted methods to prepare AgNPs, CuNPs, and NiNPs and evaluated their anti-Acanthamoeba activity.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)