期刊
TALANTA
卷 132, 期 -, 页码 809-813出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2014.10.037
关键词
Self-cleaved DNAzyme; Enzymatic cascade; L-Histidine; Exponential amplified analysis
资金
- National Science & Technology Support Program of China [2012BAC17B01]
- National Natural Science Foundation of China [21105005, 21275022, 21075011]
- Science Foundation of Hunan Provincial Education Department [12B002]
- Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation [2013CL01]
- program for new century excellent talents in university, Ministry of Education of the People's Republic of China [NCET-10-0138]
A novel strategy of cleaved DNAzyme substrate induced enzymatic cascade has been devised for the exponential amplified detection of L-histidine. The enzyme strand carries out hydrolytic cleavage of the substrate strand in the presence of L-histidine. The cleaved DNAzyme substrates introduce the polymerase/endonuclease reaction cycles as primers. The L-histidine acts as the activator for enzymatic cascade amplification generating a distinguishable fluorescence enhancement. A good nonlinear correlation (R=0.9994) between fluorescence intensity and the logarithm of the L-histidine concentration is obtained over the range from 50 nM to 1.0 mM. The detection limit was estimated as 30 nM. This efficient amplification of the fluorescence signal is attributed to the L-histidine induced cooperation of Klenow Fragment polymerase (exo(-)) and Nb.BbvCI endonuclease reaction. The activation of such enzymatic cascades through analyte-DNAzyme interactions has a substantial impact on the development of exponential amplified DNAzyme sensors. (C) 2014 Elsevier B.V. All rights reserved.
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