Article
Biochemistry & Molecular Biology
Mi-Jin Oh, Hye-Bin Lee, Guijae Yoo, Miri Park, Chang-Hyun Lee, Inwook Choi, Ho-Young Park
Summary: The study evaluated the anti-obesity effect of water-soluble red pepper leaf extract on adipocytes and HFD-fed mice. The findings showed that the extract reduced fat accumulation and improved intestinal health, suggesting its potential in preventing obesity.
Article
Biochemistry & Molecular Biology
Yuankuan Li, Sung Ho Lee, Meiyu Piao, Hyung Sik Kim, Kwang Youl Lee
Summary: This study found that Metallothionein 3 (MT3) plays an important role in the differentiation of 3T3-L1 cells into adipocytes. Overexpression of MT3 inhibited adipocyte differentiation and reduced the levels of adipogenic transcription factors C/EBPa and PPAR?. Further analysis revealed that MT3 suppressed the transcriptional activity of PPAR?, and this effect was not mediated by a direct interaction between MT3 and PPAR?. Additionally, MT3 overexpression decreased reactive oxygen species (ROS) levels during early adipocyte differentiation, while treatment with antimycin A, which induces ROS generation, restored the ROS levels.
Article
Engineering, Environmental
Yaqiang Cao, Yuanyuan Chen, Ke Miao, Shuyi Zhang, Fuchang Deng, Mu Zhu, Chao Wang, Wen Gu, Yixuan Huang, Zijin Shao, Xiaoyan Dong, Yufeng Gong, Hui Peng, Hui Yang, Yi Wan, Xudong Jia, Song Tang
Summary: Mounting evidence shows the close association between ambient PM2.5 exposure and obesity development, with adipose tissue being an important target. This study employed a preadipocyte differentiation model to comprehensively explore the adipogenic potential of PM2.5. PM2.5 exposure significantly increased fatty acid uptake, lipid accumulation, and adipogenic differentiation in a concentration-dependent manner. Transcriptome and lipidome analyses revealed disruption of transcriptional and lipid profiling at 10 μg/mL PM2.5. Activation of the peroxisome proliferator-activated receptor (PPAR) pathway and metabolism of glycerophospholipids, glycerolipids, and sphingolipids were most affected during adipocyte differentiation. The study demonstrated that PM2.5 promoted adipogenesis by activating PPAR. through reporter gene assays. The findings provide insights into PM2.5-induced adipogenesis and potential biomolecular targets for preventing PM2.5-induced obesity and related metabolic diseases.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Plant Sciences
Masao Yamasaki, Yusei Kiue, Kento Fujii, Moe Sushida, Yumi Yamasaki, Kazuhiro Sugamoto, Yosuke Suzuki, Yasuko Koga, Hisato Kunitake, Hisahiro Kai, Kenjiro Ogawa, Kazuo Nishiyama, Yo Goto, Takayuki Nakayama
Summary: Blueberry leaf extract (BLEx) has been found to suppress lipid accumulation and uric acid production in adipocytes, mainly due to the highly polymerized proanthocyanidin-rich fraction. Additionally, BLEx can inhibit uric acid production in adipocytes under hypoxic conditions.
Article
Food Science & Technology
Gavirangappa Hithamani, Ponesakki Ganesan
Summary: This study investigated the anti-adipogenic effect of polyphenols in cereal grains using 3T3-L1 adipocytes. Extracts from different cereal grains were analyzed for their polyphenolic content and composition. The results showed that polyphenols from the grains had the potential to suppress lipid accumulation, triglyceride content, and the expression of adipogenic markers in the cells. The study suggests that cereal grain polyphenols have the ability to counteract adipogenesis.
Article
Biochemistry & Molecular Biology
Hee Jung Kim, Yoon Seo Jang, Ji Won Ha, Moon-Jin Ra, Sang-Mi Jung, Jeong-Nam Yu, Kyunga Kim, Ki Hyun Kim, Sung Hee Um
Summary: The compounds isolated from Salix pseudolasiogyne twigs, oregonin and 2'-O-acetylsalicortin, were found to exhibit anti-adipogenic effects, with the latter significantly suppressing lipid accumulation at a concentration of 50 μM. These compounds have the potential to be used as therapeutic agents to effectively control adipocyte differentiation, a critical stage in the progression of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Sakan Warinhomhoun, Hnin Ei Ei Khine, Boonchoo Sritularak, Kittisak Likhitwitayawuid, Tomofumi Miyamoto, Chiaki Tanaka, Chuchard Punsawad, Yanyong Punpreuk, Rungroch Sungthong, Chatchai Chaotham
Summary: This study isolated six compounds from an orchid species and identified one compound (compound 3) with potential anti-obesity effects. Compound 3 reduced lipid storage in adipocytes and suppressed the expression of key transcription factors involved in adipocyte differentiation, as well as proteins in the MAPK pathway.
Article
Integrative & Complementary Medicine
Eunbi Koh, Boram Kim, Kyungoh Choi
Summary: The study found that Torreya nucifera seed oil (TNSO) can promote adipocyte differentiation, increase intracellular lipid accumulation, and improve adipocyte function by activating adipogenic gene expression and PPAR gamma, STAT3 pathways.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2021)
Article
Pharmacology & Pharmacy
Chaiwat Monmai, Jin-Suk Kim, Hyun Bo Sim, Doh-Won Yun, Sung-Dug Oh, Eui-Shik Rha, Jong-Jin Kim, So-Hyeon Baek
Summary: This study evaluated the effects of extracts from transgenic rice on inhibiting adipogenesis and anti-inflammatory activity. The results showed that the extracts significantly reduced lipid accumulation in cells and inhibited the expression of adipogenesis-related mRNA and inflammation-related factors. Therefore, transgenic rice may have potential as an obesity-reducing and/or -preventing agent.
Article
Biochemistry & Molecular Biology
Hee Jung Kim, Da Eun Lee, Eon Chung Park, Moon-Jin Ra, Sang-Mi Jung, Jeong-Nam Yu, Sung Hee Um, Ki Hyun Kim
Summary: Salix pseudolasiogyne, commonly known as weeping willow, has been used in traditional Korean medicine for its medicinal properties. This study isolated and identified two salicin derivatives, salicortinol and salicortin, from the twigs of S. pseudolasiogyne. Both compounds were found to significantly inhibit adipocyte differentiation in 3T3-L1 cells, suggesting their potential therapeutic use in metabolic disorders such as obesity.
Article
Toxicology
Yasukazu Takanezawa, Ryosuke Nakamura, Yuka Ohshiro, Shimpei Uraguchi, Masako Kiyono
Summary: This study investigated the potential cytotoxicity of two gadolinium-based contrast agents (GBCAs) in 12 different cell lines, especially the 3T3-L1 adipocyte cell line. The results demonstrated that these two GBCAs significantly increased intracellular gadolinium levels and suppressed adipocyte differentiation in 3T3-L1 cells. These findings contribute to the understanding of the potential toxic effects of GBCA exposure.
TOXICOLOGY LETTERS
(2023)
Article
Biology
Catarina Teixeira, Andre P. Sousa, Ines Santos, Ana Catarina Rocha, Ines Alencastre, Ana Claudia Pereira, Daniela Martins-Mendes, Pedro Barata, Pilar Baylina, Ruben Fernandes
Summary: This study demonstrates that pioglitazone enhances adipocyte differentiation and secretome production, making it an excellent model for studying adiposity. The study also proposes an improved method for adipocyte secretome production, which can be used for experimental biology studies to understand the molecular mechanisms underlying the influence of adiposity on other cells.
Article
Toxicology
Qing Li, Yan Qin Tan, Kam Kuen Cheung, Leo C. Chiu-Leung, Lai K. Leung
Summary: Recent research has indicated that exposure to environmental contaminants can lead to obesity and pose a health risk to the public. This study investigated the interaction between TRPs and specific food pollutants, finding that zeranol could enhance fat cell differentiation by antagonizing TRP-V1 and -C6 activities, leading to reduced calcium inflow and potentially promoting adipogenesis.
TOXICOLOGY LETTERS
(2021)
Article
Food Science & Technology
Na Gyeong Geum, Ho Jun Son, Joo Ho Yeo, Ju Hyeong Yu, Min Yeong Choi, Jae Won Lee, Jueng Kyu Baek, Jin Boo Jeong
Summary: HMR inhibited lipid accumulation in 3T3-L1 cells by suppressing the expression of proteins related to lipid accumulation and inducing proteasomal degradation of CEBP alpha via JNK and GSK3 beta activation.
FOOD SCIENCE & NUTRITION
(2021)
Article
Nutrition & Dietetics
Elisabetta Melloni, Silvia Grassilli, Arianna Romani, Erika Rimondi, Annalisa Marcuzzi, Enrico Zauli, Paola Secchiero, Guglielmo Paganetto, Alessandra Guerrini, Gianni Sacchetti, Massimo Tacchini
Summary: Convolvulus pluricaulis is a commonly used Indian herb in Ayurvedic medicine known for its neuroprotective and neuroinflammatory action. Recent studies have shown its involvement in lipidic and glucidic metabolism, particularly in the control of hyperlipidaemia and diabetic conditions. It has been found to promote adipocyte differentiation and regulate glucose metabolism through the modulation of key regulators such as PPAR gamma and GLUT-4. Additionally, it exhibits anti-inflammatory effects by downregulating cytokines associated with insulin resistance and glucose intolerance. These findings suggest that Convolvulus pluricaulis could have potential benefits in glycemia and may be used as an adjunctive treatment for diabetes or for better glucose control.
