Article
Multidisciplinary Sciences
Xiaowen Wang, Hong Zhang, Russell Sapio, Jun Yang, Justin Wong, Xin Zhang, Jessie Y. Guo, Sharon Pine, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian, Dimitri G. Pestov, X. F. Steven Zheng
Summary: The study reveals a crucial role of nuclear SOD1 in ribosome biogenesis and proliferation in KRAS-driven lung cancer.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Danica Tasic, Milos Opacic, Sanja Kovacevic, Aleksandra Nikolic Kokic, Milena Dimitrijevic, Dusan Nikolic, Danijela Vojnovic Milutinovic, Dusko Blagojevic, Ana Djordjevic, Jelena Brkljacic
Summary: The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are still understudied. This study found that fructose, independent of stress, decreased renal copper level and modulated renal copper metabolism to preserve vital cellular function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Achinta Sannigrahi, Sourav Chowdhury, Bidisha Das, Amrita Banerjee, Animesh Halder, Amaresh Kumar, Mohammed Saleem, Athi N. Naganathan, Sanat Karmakar, Krishnananda Chattopadhyay
Summary: Research shows that the stability and aggregation behavior of SOD1 in membrane environment are affected by Zn, not Cu. Two loop regions play a role in membrane attachment of SOD1 and aggregation driven by lipid-induced conformational changes.
Article
Chemistry, Medicinal
Courtney E. Chandler, Francisco G. Hernandez, Marissa Totten, Natalie G. Robinett, Sabrina S. Schatzman, Sean X. Zhang, Valeria C. Culotta
Summary: This study discovered a Cu-only superoxide dismutase (CaurSOD4) secreted by Candida auris and demonstrated its important role in removing superoxide ions in the host environment. CaurSOD4 selectively binds to copper and is susceptible to inhibition by specific drugs, making it a potential antifungal target.
ACS INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Tania M. Manieri, Stefano L. Sensi, Rosanna Squitti, Giselle Cerchiaro
Summary: The study altered the activity of SOD1 in AD patients by modifying its zinc complexation capacity, leading to decreased enzymatic activity even when complexed with copper. The mutant SOD1 presented instability in zinc and copper coordination, making it a valuable in vitro model for studying SOD1 behavior in AD patients.
Article
Chemistry, Physical
Norbert Lihi, Zoltan Balogh, Robert Dioszegi, Attila Forgacs, Krisztian Moldovan, Nora May, Petra Herman, Istvan Fabian, Jozsef Kalmar
Summary: The copper(II) complexes of cyclen and cyclam were covalently immobilized in mesoporous silica aerogels. The modified precursors were characterized using mass spectrometry and NMR spectroscopy. The aerogels were characterized using LV-SEM, N-2-sorption porosimetry, FTIR, EPR, and SANS. The immobilization resulted in enhanced SOD activity and the aerogels have the potential to be used as antioxidant pharmaceutical agents.
APPLIED SURFACE SCIENCE
(2023)
Review
Cell Biology
Xinyue Chen, Qi Cai, Ruikai Liang, Deju Zhang, Xiao Liu, Meiying Zhang, Yan Xiong, Minxuan Xu, Qi Liu, Pengyang Li, Peng Yu, Ao Shi
Summary: Copper is a vital mineral for normal physiological processes, including cardiovascular systems. However, copper-induced cell death has been recognized as an important factor in the pathogenesis and progression of cardiovascular disease. Understanding the mechanisms of copper-induced cell death can help improve cardiovascular disease management. This review explores the relationship between copper homeostasis and pathways related to copper-induced cell death in cardiovascular disease, as well as therapeutic strategies.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Angelique N. Besold, Vinit Shanbhag, Michael J. Petris, Valeria C. Culotta
Summary: Copper is essential for all organisms, with ceruloplasmin being the most abundant extracellular copper-binding molecule in serum. Studies suggest that ceruloplasmin may be a preferred source of copper for fungal pathogens.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Li-Qiang Wang, Yeyang Ma, Han-Ye Yuan, Kun Zhao, Mu-Ya Zhang, Qiang Wang, Xi Huang, Wen-Chang Xu, Bin Dai, Jie Chen, Dan Li, Delin Zhang, Zhengzhi Wang, Liangyu Zou, Ping Yin, Cong Liu, Yi Liang
Summary: The authors determined the cryo-EM structure of SOD1 fibrils, providing insights into the conversion of SOD1 during the pathogenesis of ALS.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Paolo Pastorino, Elisabetta Pizzul, Damia Barcelo, Maria Cesarina Abete, Gabriele Magara, Paola Brizio, Rosa Avolio, Marco Bertoli, Alessandro Dondo, Marino Prearo, Antonia Concetta Elia
Summary: This study investigated the oxidative stress ecology in Danube barbel from the Barbucina creek, analyzing the levels of antioxidant biomarkers and trace elements in the fish. Results indicated a significant association between certain trace elements and enzyme activity, suggesting their role as stressors in the aquatic environment. The study provides insights into the biochemical defensive response of fish to environmental disturbances, advancing our understanding of the effects of environmental factors on oxidative stress in aquatic ecosystems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Physiology
Micaela K. Roy, Francesca Cendali, Gabrielle Ooyama, Fabia Gamboni, Holmes Morton, Angelo D'Alessandro
Summary: In the study on PKD patients, metabolomics analyses revealed metabolic disruptions including reduced late glycolysis, accumulation of PPP metabolites, oxidant stress, elevated hypoxia markers, and membrane lipid oxidation and remodeling. This study highlights the importance of basic science research in investigating pathologies in underrepresented minorities and aims to target treatments to health disparities more effectively.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Enrico Luchinat, Matteo Cremonini, Lucia Banci
Summary: Understanding the complex processes that keep cells and organisms alive is essential for comprehending diseases and developing new drugs. Solution NMR is a key technique to investigate the structure and dynamic behavior of biological macromolecules within living cells at high resolution.
Review
Chemistry, Multidisciplinary
Enrico Luchinat, Matteo Cremonini, Lucia Banci
Summary: A detailed understanding of the complex processes that contribute to the functionality of cells and organisms is crucial for disease comprehension and drug development. Among the available structural techniques, solution NMR is the only method capable of investigating macromolecular structures and dynamic behaviors directly within living cells. Over the past 20 years, in-cell NMR has emerged as a powerful tool, allowing researchers to study cell samples, isotopic labeling, and bioreactor devices, thus enhancing the lifetime of cells for real-time monitoring of intracellular metabolites and proteins.
Review
Chemistry, Multidisciplinary
Benjamin G. Trist, James B. Hilton, Dominic J. Hare, Peter J. Crouch, Kay L. Double
Summary: Cu/Zn superoxide dismutase (SOD1) is a crucial antioxidant enzyme in eukaryotic life, with mitochondrial aerobic respiration increasing cellular superoxide production and reliance on SOD1. Diseases of the central nervous system often involve disruptions in SOD1 biology, revealing complex molecular characteristics that differentiate healthy from disease-contributing SOD1 proteins. This review highlights current knowledge of SOD1 biology, from genetics to protein function and stability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Liangzhong Lim, Jian Kang, Jianxing Song
Summary: This study reveals the role of cations in the initial folding of hSOD1. Most cations, including Zn2+ mimics, showed negligible binding or induction for folding of nascent hSOD1. Cu2+ exhibited extensive binding to the unfolded state but led to severe aggregation. Surprisingly, Fe2+ was found to have Zn2+-like folding-inducing capacity.
SCIENTIFIC REPORTS
(2023)