Pseudorhizobium pelagicum gen. nov., sp nov isolated from a pelagic Mediterranean zone

Two novel Alphaproteobacteria strains, R1-20084(T) and R2-400B4, were isolated from the Mediterranean Sea off the coast of Alicante in Spain. The phylogenetic analysis of the 16S rRNA gene showed that they are related to members of Family Rhizobiaceae. The 16S rRNA gene sequence of strain R1-200B4(T) presents 97.0% and 95.6% similarity with respect to the type strains of the type species from genera Neorhizobium and Rhizobium. Neorhizobium galegae HAMBI 540(T) and Rhizobium leguminosarum USDA 2370(T), respectively. The remaining genera of family Rhizobiaceae showed similarities lower than 95%. The recA and atpD gene sequences of strain R1-2008B4(T) showed, respectively, 90% and 88.6% similarity with respect to N. galegae HAMBI 540(T) and 87% and 86% with respect to R. leguminosarum USDA 2370(T). The calculated ANI values between the genomes of the strain R1-200B4(T) and those of N. galegae HAMBI 540(T) and R. leguminosarum 3841 are 75.9% and 74.0%, respectively. The major fatty acids are those from summed feature 8 (C-18:1 omega 6c/C-18:1 omega 7c) and the C-16:0. Catalase and oxidase were positive. Nitrate reduction and aesculin hydrolysis were positive. Production of beta-galactosidase and urease was positive. The production of indol, arginine dehydrolase or gelatinase was negative. Growth was observed in presence of 7% NaCI. Therefore, based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, we propose to classify the strains isolated in this study in a new genus named Pseudorhizobium gen. nov. and a new species named Pseudorhizobium pelagicum sp. nov. with the type strain R1-200B4(T) (= LMG 28314(T) =CECT 8629(T)). (C) 2015 Elsevier GmbH. All rights reserved.