Article
Biochemical Research Methods
Ryan A. Groves, Carly C. Y. Chan, Spencer D. Wildman, Daniel B. Gregson, Thomas Rydzak, Ian A. Lewis
Summary: Liquid chromatography mass spectrometry (LC-MS) is a widely used strategy for metabolomics analyses, offering the potential for both biomarker discovery and clinical diagnostics. However, clinical applications of LC-MS require higher throughput, which has been limited by chromatographic gradient lengths. To address this issue, researchers have developed a new analytical strategy called sequential quantification using isotope dilution (SQUID), which enables high-throughput quantification of target biomarkers in complex samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agronomy
Ni Zhang, Weiwen Tan, Guimei Luo, Tianyi Pu, Jinqiu Wang, Xianhu Zhang, Yuehua Song
Summary: Camellia tetracocca, a unique tea species in southwest China, is frequently attacked by the pest Empoasca onukii. The metabolic mechanisms of C. tetracocca's defense against this pest are still unclear. Through comparative analysis using GC-MS and LC-MS, differential metabolites were identified, showing that accumulation of specialized metabolites may contribute to the resistance of C. tetracocca against E. onukii. These specialized metabolites have the potential to be used as biocontrol agents against the pest.
Article
Ophthalmology
Xiaoyan Han, Lili Zhang, Lingjie Kong, Ming Tong, Zehui Shi, Xiu Miao Li, Ting Zhang, Qin Jiang, Yan Biao
Summary: Diabetic retinopathy (DR) is a common blind-causing ophthalmic disease associated with diabetes mellitus. Metabolomics analysis revealed 311 differential metabolites between diabetic retinas and non-diabetic retinas, with purine metabolism, amino acid metabolism, and glycerophospholipid metabolism being the most affected pathways. Among the purine metabolites, adenosine, guanine, and inosine showed higher sensitivity, specificity, and accuracy for predicting DR.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Joao P. P. Vieira, Filip Ottosson, Amra Jujic, Vladimir Denisov, Martin Magnusson, Olle Melander, Joao M. N. Duarte
Summary: Combining LC-MS and NMR techniques improves the discrimination of metabolic disease development, including those induced by a high-fat diet. The study confirms that metabolic pathways in mice affected by high-fat diet also impact diabetes in humans.
Article
Chemistry, Analytical
Sylvia K. Neef, Ute Hofmann, Thomas E. Muerdter, Matthias Schwab, Mathias Haag
Summary: The suitability of a capillary hydrophilic interaction chromatography (CapHILIC) -QTOF-MS system for non-targeted metabolomics was evaluated and compared with a narrow-bore hydrophilic interaction chromatography (HILIC) -QTOF-MS system. Both platforms detected a similar number of features and performed well in terms of retention time stability, analytical repeatability, and signal intensity. The use of CapHILIC increased the signal areas of all metabolites, though the signal-to-noise ratio was only improved for 50% of the metabolites. Further optimization of CapHILIC conditions improved reproducibility and signal intensity.
Article
Plant Sciences
Manamele Dannies Mashabela, Fidele Tugizimana, Paul Anton Steenkamp, Lizelle Ann Piater, Ian Augustus Dubery, Msizi Innocent Mhlongo
Summary: This study used LC-MS technology to analyze the metabolomic responses of Pst-resistant and susceptible wheat varieties to P. striiformis infection. The results showed that wheat plants undergo time-dependent metabolic reprogramming in response to the pathogen, with changes in phenylpropanoids, amino acids, lipids, benzoic acids, TCA cycle intermediates, and benzoxazinoids. The resistant cultivar Koonap accumulated defense metabolites more rapidly than the susceptible Gariep cultivar, suggesting the potential for metabolic engineering to improve plant tolerance and resistance to biotic stresses.
Article
Biotechnology & Applied Microbiology
Kanhaiya Kumar, Per Bruheim
Summary: In this study, the impact of nitrogen and phosphate limitation on the metabolite pool levels in Streptomyces coelicolor was investigated. Nitrogen limitation resulted in decreased metabolite levels and lowered respiration, while phosphate limitation led to downregulation of phosphorylated metabolite pools. Additionally, carbon isotope labeling experiments revealed simultaneous glycolysis and gluconeogenesis during the co-utilization of glucose and glutamate.
Article
Food Science & Technology
Asmaa M. Otify, Shahira A. ElBanna, Basma M. Eltanany, Laura Pont, Fernando Benavente, Rana M. Ibrahim
Summary: This study characterized the metabolite profiles of six lettuce varieties and established their correlation with antivirulence effects. Various metabolites were identified, and red varieties showed higher levels of chlorogenic and chicoric acids indicating antioxidant properties. Romaine longifolia exhibited enrichment in amino acids and disaccharides, while crispa, capitata, and lolla bionda had a high content of β-carboline alkaloids. All varieties demonstrated antibacterial potential against Staphylococcus aureus strains, and specific bioactives such as sesquiterpenes, β-carboline alkaloids, amino acids, and oxy-fatty acids were identified. The results highlight the significance of lettuce as a functional food source and suggest breeding programs should focus on varieties rich in antibacterial agents.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Bo-Min Kim, Hyeon-Jeong Lee, Yeong H. Song, Hyun-Jin Kim
Summary: Increasing NaCl concentration led to decreased growth and mineral imbalance in spinach plants. NaCl irrigation resulted in decreased levels of most metabolites, affecting the nutritional quality of spinach.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Article
Endocrinology & Metabolism
Tiina Jaeaeskelaeinen, Olli Kaerkkaeinen, Jenna Jokkala, Anton Klavus, Seppo Heinonen, Seppo Auriola, Marko Lehtonen, Kati Hanhineva, Hannele Laivuori
Summary: Maternal metabolism undergoes significant changes during pregnancy, yet few studies have explored these changes using metabolomics technologies. We utilized LC-MS based non-targeted metabolomics to compare the metabolic profiles of serum samples from pre-eclamptic and healthy women in early and late pregnancy. Our study found that the progression of pregnancy had a major impact on serum metabolite profiles, with distinct changes observed between early and late pregnancy samples. The metabolic signatures of pregnancy were largely similar between pre-eclamptic and healthy women, with only a few specific metabolites showing differences.
Article
Food Science & Technology
Pufan Zheng, Mei Zhang, Xin Fang, Lili Tang, Zhixue Wang, Fuchen Shi
Summary: This study identified and quantified metabolites in three pear species using a targeted LC-MS/MS approach. The results showed distinct differences in metabolite composition between cultivars, and identified metabolic pathways related to fruit quality. D-xylose, formononetin, procyanidin A1 and beta-nicotinamide mononucleotide were identified as major differentially expressed metabolites.
Article
Biochemical Research Methods
Fabio Neves dos Santos, Dilze Maria Argolo Magalhaes, Edna Dora Martins Newman Luz, Marcos Nogueira Eberlin, Ana Valeria Colnaghi Simionato
Summary: The LC-MS metabolomic screening analysis helps distinguish resistant and susceptible cacao genotypes infected with Ceratocystis cacaofunesta, aiding in identifying the best strategy to combat Ceratocystis wilt.
Article
Chemistry, Applied
Mengyu Chen, Wenjie Huang, Zhibin Yin, Wenyang Zhang, Qian Kong, Shaowen Wu, Wenyan Li, Zhao Bai, Alisdair R. Fernie, Xiaodan Huang, Shijuan Yan
Summary: The research found metabolic differences among black wolfberry fruits grown in different geographical regions, with some samples showing significantly different metabolite profiles with higher levels of beneficial components, providing a new direction for further studies on the health benefits of black wolfberry fruits.
Article
Obstetrics & Gynecology
Elham Hosseini, Naser Amirjannati, Ralf Henkel, Mahshid Bazrafkan, Hanieh Moghadasfar, Kambiz Gilany
Summary: This study investigated the metabolome of human seminal plasma (HSP) in infertile patients with teratozoospermia compared to fertile normozoospermic men. Metabolic analysis revealed significant differences in amino acid profiles between the two groups, with increased levels of glutamine and asparagine, and decreased levels of cysteine, tryptophan, glycine, and valine in teratozoospermic samples. Random forest analysis identified a set of 15 metabolites that were strong predictors of teratozoospermia. The altered amino acid metabolome in teratozoospermia may provide valuable insights into the etiology of male infertility and potential biomarkers for therapeutic targets.
REPRODUCTIVE SCIENCES
(2023)
Review
Food Science & Technology
Charlotte Floret, Anne-Flore Monnet, Valerie Micard, Stephane Walrand, Camille Michon
Summary: With the global population growing, it is important to balance the intake of animal and vegetable protein sources and limit overall protein resources. This article analyzes the protein concentration and essential amino acids of different protein sources to meet human needs. New classification indexes are proposed, considering the influence of protein gelation in food formulation, and improvements are suggested to fulfill human nutritional requirements.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Nutrition & Dietetics
Quentin Leyrolle, Renata Cserjesi, Romane Demeure, Audrey M. Neyrinck, Camille Amadieu, Julie Rodriguez, Olli Kaerkkaeinen, Kati Hanhineva, Nicolas Paquot, Miriam Cnop, Patrice D. Cani, Jean-Paul Thissen, Laure B. Bindels, Olivier Klein, Olivier Luminet, Nathalie M. Delzenne
Summary: This study evaluated the potential links between serum metabolomics and gut microbiome, and mood disturbances in obese patients. The findings revealed altered levels of microbiota-derived metabolites in obese subjects with mood disturbances, suggesting new targets for tackling mood disorders in this context.
Article
Biochemistry & Molecular Biology
Ambrin Farizah Babu, Ville Mikael Koistinen, Soile Turunen, Gloria Solano-Aguilar, Joseph F. Urban Jr, Iman Zarei, Kati Hanhineva
Summary: In this study, a sensitive and simple qualitative LC-MS/MS method was developed for the identification and distribution of sterols, bile acids, and acylcarnitines in biological samples. The method allowed the simultaneous detection and annotation of these metabolites from reference standards and different biological samples, providing a valuable tool for screening these metabolites in routine scientific research.
Review
Endocrinology & Metabolism
Retu Haikonen, Olli Karkkainen, Ville Koistinen, Kati Hanhineva
Summary: 5-Aminovaleric acid betaine (5-AVAB) is a gut microbiota-associated compound that accumulates in metabolically active tissues. It has been found in higher concentrations in the heart, muscle, and brown adipose tissue. It has positive health effects on fetal brain development, insulin secretion, and reduced cancer risk, but it is also associated with negative health outcomes such as cardiovascular disease and fatty liver disease.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Multidisciplinary Sciences
Elise Nordin, Stine K. Steffensen, Bente B. Laursen, Sven-Olof Andersson, Jan-Erik Johansson, Per Aman, Goran Hallmans, Michael Borre, Dan Staerk, Kati Hanhineva, Inge S. Fomsgaard, Rikard Landberg
Summary: In this study, a quantitative method for analysis of benzoxazinoids (BXs) was established, and the plasma levels of BXs after consumption of whole grain/bran rye or refined wheat were investigated in men with prostate cancer. The results showed that BX metabolites were significantly higher after rye consumption compared to refined wheat. Additionally, four BX-related metabolites were inversely associated with PSA levels in plasma. These findings suggest that rye may have potential preventive effects against prostate cancer.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Ambrin Farizah Babu, Susanne Csader, Ville Mannisto, Milla-Maria Tauriainen, Heikki Pentikainen, Kai Savonen, Anton Klavus, Ville Koistinen, Kati Hanhineva, Ursula Schwab
Summary: This study investigated the metabolic changes associated with non-alcoholic fatty liver disease (NAFLD) in response to high-intensity interval training (HIIT) intervention. The results showed that HIIT significantly improved clinical parameters of NAFLD patients, including fasting plasma glucose concentration and waist circumference. Metabolomics analysis revealed sample-type-specific changes in metabolite levels, with amino acids and their derivatives accumulating in adipose tissue and plasma, while decreasing in urine and stool. The study highlights the role of adipose tissue in responding to HIIT and suggests that altered amino acid metabolism in adipose tissue may play a critical role in improving fasting plasma glucose concentration.
SCIENTIFIC REPORTS
(2022)
Article
Nutrition & Dietetics
Stefania Noerman, Jyrki K. Virtanen, Marko Lehtonen, Carl Brunius, Kati Hanhineva
Summary: The study aimed to identify fasting serum metabolites associated with whole grain intake in a free-living population. The findings suggested strong associations between whole grain intake and several metabolites, which remained significant even after adjusting for potential confounders. These metabolites have the potential to serve as robust biomarkers for whole grain consumption.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Environmental Sciences
T. Schillemans, I. A. Bergdahl, K. Hanhineva, L. Shi, C. Donat-Vargas, J. Koponen, H. Kiviranta, R. Landberg, A. akesson, C. Brunius
Summary: The widespread environmental pollutants PFAS have been repeatedly linked to elevated serum cholesterol in humans, but the underlying mechanisms are still unclear. In this study, we investigated the associations between PFAS-related metabolite features and plasma cholesterol and triglyceride concentrations. The results indicated that longer chain PFAS may affect glycerophospholipid metabolism, leading to changes in triglyceride levels.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Valeria Iannone, Johnson Lok, Ambrin Farizah Babu, Carlos Gomez-Gallego, Roosa Maria Willman, Ville Mikael Koistinen, Anton Klavus, Mikko I. Kettunen, Anna Karlund, Ursula Schwab, Kati Hanhineva, Marjukka Kolehmainen, Hani El-Nezami
Summary: This study aimed to elucidate the pathogenesis of NAFLD and its relationship with metabolic alterations. ALIOS diet-fed mice showed higher hepatic steatosis, body weight, energy consumption, and liver mass compared to control mice. The ALIOS diet also altered the expression of genes related to inflammation and lipid metabolism. Metabolomics analysis revealed changes in lipid and peptide profiles, as well as correlations between different metabolites and inflammation, lipid uptake, and synthesis. The reduction of antioxidant metabolites and gut microbiota-derived metabolites contributed to NAFLD development. Non-targeted metabolomics combined with gene expression can help identify potential therapeutic targets for NAFLD.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Editorial Material
Endocrinology & Metabolism
Ville Koistinen, Olli Kaerkkaeinen, Pekka Keski-Rahkonen, Hiroshi Tsugawa, Augustin Scalbert, Masanori Arita, David Wishart, Kati Hanhineva
Summary: The metabolomics literature needs improved nomenclature for individual metabolites to ensure consistency and avoid misinterpretations. This Comment proposes recommendations for metabolite annotations to encourage the scientific community and publishers to adopt a more standardized approach.
Article
Biology
Aleksi Husso, Tiina Pessa-Morikawa, Ville Mikael Koistinen, Olli Karkkainen, Hyuk Nam Kwon, Leo Lahti, Antti Iivanainen, Kati Hanhineva, Mikael Niku
Summary: Maternal microbiota has a significant impact on fetal development, with gene expression differences in the fetal intestines, brains, and placenta associated with microbial metabolite concentrations. Male fetuses may be more susceptible to microbial modulation, affecting genes important for immune system, neurophysiology, translation, and energy metabolism before birth. Several microbial metabolites have been identified in this context, with many important metabolites yet to be discovered.
Article
Nutrition & Dietetics
Mari Naatanen, Anna Karlund, Santtu Mikkonen, Anton Klavus, Otto Savolainen, Marko Lehtonen, Leila Karhunen, Kati Hanhineva, Marjukka Kolehmainen
Summary: This study investigated the effect of two isocaloric 24-week weight maintenance diets on metabolism and identified metabolite features associated with successful weight loss maintenance. It was found that the dietary fiber, protein, and fat content of the diet influenced amino acid and lipid metabolism, and increased abundance of certain phospholipids and free fatty acids was associated with better weight loss maintenance.
CLINICAL NUTRITION
(2023)
Article
Cell Biology
Mueez U-Din, Vanessa D. de Mello, Marjo Tuomainen, Juho Raiko, Tarja Niemi, Tobias Fromme, Anton Klavus, Nadine Gautier, Kimmo Haimilahti, Marko Lehtonen, Karsten Kristiansen, John W. Newman, Kirsi H. Pietilaeinen, Jussi Pihlajamaeki, Ez-Zoubir Amri, Martin Klingenspor, Pirjo Nuutila, Eija Pirinen, Kati Hanhineva, Kirsi A. Virtanen
Summary: Cold stimulates NAD+ metabolism in humans and may be regulated by multiple tissues.
Article
Endocrinology & Metabolism
Valeria Iannone, Ambrin Farizah Babu, Johnson Lok, Carlos Gomez-Gallego, Giuseppe D'Auria, Ruben Vazquez-Uribe, Troels Holger Vaaben, Mareike Bongers, Santtu Mikkonen, Maija Vaittinen, Ida Tikkanen, Mikko Kettunen, Anton Klavus, Ratika Sehgal, Dorota Kaminska, Jussi Pihlajamaki, Kati Hanhineva, Hani El-Nezami, Morten Otto Alexander Sommer, Marjukka Kolehmainen
Summary: This study found that Escherichia coli Nissle 1917 expressing aldafermin, combined with dietary changes, can alleviate symptoms of MASLD, including reducing body weight, liver steatosis, plasma aspartate aminotransferase, and plasma cholesterol levels. RNA sequencing and metabolomics analysis revealed the downregulation of amino acid metabolism and related receptor signaling pathways, which may be implicated in the reduction of hepatic steatosis and insulin resistance.
MOLECULAR METABOLISM
(2023)
Review
Biochemistry & Molecular Biology
Maaria Kortesniemi, Stefania Noerman, Anna Karlund, Jasmin Raita, Topi Meuronen, Ville Koistinen, Rikard Landberg, Kati Hanhineva
Summary: Metabolomics has become a crucial method in nutrition research, allowing for the discovery and validation of dietary biomarkers and playing an important role in understanding the activity and functionality of gut microbiota. The field has also seen emerging developments in precision nutrition.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)