Article
Immunology
Jun-meng Wang, Si-rui Lin, Yuan-bing Zhu, Jing Yuan, Yue-mei Wang, Qun Zhang, Lu-shuang Xie, Si-hui Li, Shu-qing Liu, Shu-guang Yu, Qiao-feng Wu
Summary: This study identified 2597 acetylation events and 1914 sites in the UC model, revealing enrichment of differentially acetylated proteins in pathways like TCA cycle, fatty acid metabolism, and protein processing in the endoplasmic reticulum. Furthermore, it showed the potential role of HSP90B1-K142ac in the pathogenesis of UC.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yongxu Zhao, Shuang Li, Yanhao Chen, Yuchen Wang, Yuda Wei, Tingting Zhou, Yuwei Zhang, Yuanyuan Yang, Lanlan Chen, Yan Liu, Cheng Hu, Ben Zhou, Qiurong Ding
Summary: The glucagon-PKA signal plays a dual role in controlling hepatic gluconeogenesis in mice. It directly stimulates histone phosphorylation for gluconeogenic gene regulation in the fasting state, and inhibits it in the fed state. The hormone signal is transmitted to chromatin for rapid and efficient activation of gluconeogenic genes.
Article
Chemistry, Multidisciplinary
Soeren Kirchgaessner, Michael B. Braun, Natascha Bartlick, Cengiz Koc, Christopher D. Reinkemeier, Edward A. Lemke, Thilo Stehle, Dirk Schwarzer
Summary: Lysine acetylation is a post-translational modification of proteins, and a 1,2,4-triazole amino acid (ApmTri) has been identified as a mimic for acetyllysine (Kac) to recruit bromodomains (Brds) of the BET family. The optimization of ApmTri substituents and side chain spacing enabled BET Brds to bind to ApmTri-containing peptides with affinities similar to native substrates. The use of genetically encoded ApmTri allowed the study of BET proteins in vitro and in vivo by providing a stable Kac mimic that reflects charge neutralization and Brd recruitment.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medical Laboratory Technology
Maria Del Carmen Navarro-Ruiz, Jaime Lopez-Alcala, Alberto Diaz-Ruiz, Sandra Diaz Del Moral, Carmen Tercero-Alcazar, Andrea Nieto-Calonge, Jose Lopez-Miranda, Francisco J. Tinahones, Maria M. Malagon, Rocio Guzman-Ruiz
Summary: Obesity is a rapidly growing pathological condition worldwide, with altered lipid accumulation in adipose tissue as one of the main causes. This study explores the molecular mechanisms of adipose tissue dysfunction in conditions of obesity and insulin resistance through the study of protein acetylation modifications. The results reveal different acetylation patterns in subcutaneous and omental adipose tissue, and the impact of acetylation on the function of FABP4, highlighting the molecular complexity of adipose tissue.
TRANSLATIONAL RESEARCH
(2022)
Article
Biochemical Research Methods
Fangjiao Lv, Yang Xu, Dean W. Gabriel, Xue Wang, Ning Zhang, Wenxing Liang
Summary: Lysine acetylation plays an important role in the sporulation process of F. oxysporum, affecting the function of various cellular proteins. The changes in Kac levels of three endoplasmic reticulum-resident molecular chaperones were studied, and their importance in proper sporulation was identified.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Krista Fleck, Seth McNutt, Feixia Chu, Victoria Jeffers
Summary: TgBDP1 is a critical regulator of gene expression in Toxoplasma gondii and loss of TgBDP1 leads to global dysregulation of gene expression. TgBDP1 interacts with other bromodomain proteins and various transcriptional regulatory complexes.
Article
Microbiology
Kristen M. Jew, Van Thi Bich Le, Kiana Amaral, Allysa Ta, Nina M. Nguyen May, Melissa Law, Nicole Adelstein, Misty L. Kuhn
Summary: Acetylation is a protein post-translational modification that can influence cellular processes. Two mechanisms of N epsilon-acetylation have been identified in bacteria - non-enzymatic/chemical acetylation and enzymatic acetylation. This study focused on determining the 3D location of lysine residues that are acetylated by E. coli enzymes and investigating their conservation in homologs. The results revealed discrepancies between linear amino acid sequences and 3D structures in assessing lysine residue conservation.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anna-Theresa Blasl, Sabrina Schulze, Chuan Qin, Leonie G. Graf, Robert Vogt, Michael Lammers
Summary: Ac(et)ylation of lysine side chains is a dynamic post-translational modification that regulates fundamental cellular processes and affects the ageing process of organisms. Sirtuins have been identified as mediators of the beneficial effects of caloric/dietary restriction on health or lifespan. The focus is on sirtuins and lysine acyltransferases as direct sensors and mediators of cellular metabolic state.
BIOLOGICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Zhiqiang Lu, Xiaomeng Xu, Dongmei Li, Na Sun, Songyi Lin
Summary: The study demonstrated that sea cucumber peptides can prevent memory impairment by increasing the acetylation level of histones and affecting memory-related pathways. This neuroprotective effect is achieved through regulating post-transcriptional hippocampal protein acetylation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Nour Fatema, Chenguang Fan
Summary: Lysine acetylation is a common post-translational modification that plays a significant role in biological pathways. Proteomic analyses have identified numerous acetylation sites, but the specific functions of individual acetylation events remain unclear. To address this issue, the genetic code expansion technique has been utilized to generate site-specifically acetylated proteins, enabling the characterization of acetylation effects with minimal interferences. In this review, we summarize the development of this technique for lysine acetylation and discuss recent studies on lysine acetylation of citrate acid cycle enzymes in bacteria, highlighting its practical application in protein acetylation studies.
MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Eunji Sung, Hyunchae Sim, Young-Chang Cho, Wonhwa Lee, Jong-Sup Bae, Minjia Tan, Sangkyu Lee
Summary: In this study, the authors identified acetylation and lactylation sites in Kupffer cells using global acylome technology. They found differences in the subcellular distributions and sequence motifs of modified proteins. Functional enrichment analyses provided insights into the roles of these modifications.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xuan Ma, Qingxiao Jia, Sheng Li, Zhengting Chen, Xin Ming, Yu Zhao, Dao-Xiu Zhou
Summary: Heterosis refers to the superior performance of a hybrid compared with its parental lines. In this study, we investigated the molecular mechanisms underlying heterosis in the elite hybrid rice Shanyou 63. We found that the superior growth of the hybrid was associated with the accumulation of growth-promoting and energy metabolism proteins, enhanced energy metabolism activities, and increased protein lysine acetylation. The findings suggest that an enhanced network of energy-producing metabolic activity and growth-promoting histone acetylation/gene expression in the hybrid may contribute to its superior growth rate.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Shivani Nanda, Marc-Antoine Jacques, Wen Wang, Chad L. Myers, L. Safak Yilmaz, Albertha J. M. Walhout
Summary: A new study reveals that three-quarters of metabolic genes in the nematode Caenorhabditis elegans are transcriptionally regulated, and generates a co-regulated subpathway map of metabolism. The researchers also develop a web application to query gene associations with metabolic pathways. This study sheds light on the ubiquity of transcriptional regulation in metabolism and provides a blueprint for similar studies in other organisms.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Review
Microbiology
Michael Lammers
Summary: Ac(et)ylation is a significant post-translational modification affecting essential cellular processes in all domains of life. It can occur at the ε-amino group of lysine side chains or at the α-amino group of proteins, and is involved in regulating protein function through various mechanisms including charge quenching, size alteration of lysine side chains, and interference with other modifications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Navjyoti Chakraborty, Priyanka Sharma, Kostya Kanyuka, Ravi Ramesh Pathak, Devapriya Choudhury, Richard Hooley, Nandula Raghuram
PLANT MOLECULAR BIOLOGY
(2015)
Article
Multidisciplinary Sciences
Navjyoti Chakraborty, Priyanka Sharma, Kostya Kanyuka, Ravi R. Pathak, Devapriya Choudhury, Richard A. Hooley, Nandula Raghuram
Review
Oncology
Ravi Pathak, Marc Philizaire, Shiraz Mujtaba
Article
Plant Sciences
Annie P. Jangam, Ravi R. Pathak, Nandula Raghuram
FRONTIERS IN PLANT SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Ravi Ramesh Pathak, Sunila Lochab
CANADIAN JOURNAL OF MICROBIOLOGY
(2010)
Review
Cell Biology
Ravi Ramesh Pathak, Vrushank Dave
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2014)
Review
Chemistry, Multidisciplinary
Vladimir N. Uversky, Vrushank Dave, Lilia M. Iakoucheva, Prerna Malaney, Steven J. Metallo, Ravi Ramesh Pathak, Andreas C. Joerger
Article
Biochemistry & Molecular Biology
Jaymin J. Kathiriya, Ravi Ramesh Pathak, Eric Clayman, Bin Xue, Vladimir N. Uversky, Vrushank Dave
MOLECULAR BIOSYSTEMS
(2014)
Article
Multidisciplinary Sciences
Prerna Malaney, Ravi Ramesh Pathak, Bin Xue, Vladimir N. Uversky, Vrushank Dave
SCIENTIFIC REPORTS
(2013)
Article
Multidisciplinary Sciences
Jaymin J. Kathiriya, Ravi Ramesh Pathak, Alexandr Bezginov, Bin Xue, Vladimir N. Uversky, Elisabeth R. M. Tillier, Vrushank Dave
Article
Biochemistry & Molecular Biology
Jaymin J. Kathiriya, Ravi Ramesh Pathak, Alexandr Bezginov, Bin Xue, Vladimir N. Uversky, Elisabeth R. M. Tillier, Vrushank Dave
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2017)
Article
Biochemistry & Molecular Biology
Suman K. Das, Ravi Ramesh Pathak, Devapriya Choudhury, Nandula Raghuram
MOLECULAR GENETICS AND GENOMICS
(2007)