期刊
NUCLEIC ACIDS RESEARCH
卷 43, 期 2, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku1094
关键词
-
资金
- European Community [241955]
- SYSCILIA
- International Cancer Genome Sequencing (ICGC) MMML Seq project [01KU1002A, 01KU1002B, 01KU1002C, 01KU1002D, 01KU1002E, 01KU1002F, 01KU1002G, 01KU1002H, 01KU1002I, 01KU1002J]
- DFG (Deutscheforschung gemeinschaft) - German Science Ministry as part of the Cell Networks Excellence Initiative
Systematic interrogation of mutation or protein modification data is important to identify sites with functional consequences and to deduce global consequences from large data sets. Mechismo (mechismo.russellab.org) enables simultaneous consideration of thousands of 3D structures and biomolecular interactions to predict rapidly mechanistic consequences for mutations and modifications. As useful functional information often only comes from homologous proteins, we benchmarked the accuracy of predictions as a function of protein/structure sequence similarity, which permits the use of relatively weak sequence similarities with an appropriate confidence measure. For protein-protein, protein-nucleic acid and a subset of protein-chemical interactions, we also developed and benchmarked a measure of whether modifications are likely to enhance or diminish the interactions, which can assist the detection of modifications with specific effects. Analysis of high-throughput sequencing data shows that the approach can identify interesting differences between cancers, and application to proteomics data finds potential mechanistic insights for how post-translational modifications can alter biomolecular interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据