Article
Cell Biology
Neha Gupta, Cristina Soriano-Ubeda, Paula Stein, Virginia Savy, Brian N. Papas, Goli Ardestani, Ingrid Carvacho, Dominique Alfandari, Carmen J. Williams, Rafael A. Fissore
Summary: TRPM7 is a crucial chanzyme for embryo development. It maintains magnesium (Mg2+) homeostasis, prevents oxidative stress, and promotes gene expression necessary for developmental progression and cell-lineage specification.
Article
Biochemistry & Molecular Biology
Asfree Gwanyanya, Inga Andriule, Bogdan M. Istrate, Farjana Easmin, Kanigula Mubagwa, Regina Macianskiene
Summary: TRPM6 and TRPM7 proteins in the heart may be the molecular candidates for native cardiac Mg2+-sensitive channels, potentially influencing the duration of action potentials and having implications in arrhythmogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kirill D. Nadezhdin, Leonor Correia, Chamali Narangoda, Dhilon S. Patel, Arthur Neuberger, Thomas Gudermann, Maria G. Kurnikova, Vladimir Chubanov, Alexander I. Sobolevsky
Summary: The transient receptor potential channel TRPM7 is a crucial regulator of divalent cation balance in the body, impacting various biological processes such as embryonic development, immune responses, and cell functionalities. It is linked to neuronal and cardiovascular disorders as well as tumor progression, highlighting its potential as a drug target. Using cryo-EM, functional analysis, and molecular dynamics simulations, researchers have unraveled two distinct structural mechanisms behind TRPM7 activation through gain-of-function mutations and the agonist naltriben. These mechanisms exhibit different conformational dynamics and involve different domains. Additionally, the study identifies a binding site for potent and selective inhibitors that stabilize the closed state of TRPM7, paving the way for the development of drugs targeting TRPM7-related channelopathies.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Xuebo Wei, Zhiyong Liao, Liangliang Yang, Fangfang Wu, Shaodong Chen, Chuxiao Shao, Xin Wang, Keshen Xiao, Jian Xiao, Ke Xu, Da Sun, Hongyu Zhang
Summary: The study found that phosphorus magnesium fiber (PMF), as a novel nanoscale functional material, can accelerate wound healing. PMF releases magnesium ions into wounds, which promote wound closure and regulate macrophage polarization. This research provides insights for the potential use of PMF in wound healing and tissue regeneration.
APPLIED MATERIALS TODAY
(2023)
Article
Biology
Tomohiro Numata, Kaori Sato-Numata, Meredith C. Hermosura, Yasuo Mori, Yasunobu Okada
Summary: Animal cells can regulate their volume after swelling through the regulatory volume decrease (RVD) mechanism, in which TRPM7 plays a crucial role in enhancing VSOR activity and LRRC8A expression. TRPM7 facilitates VSOR activity by increasing the expression of LRRC8A mRNA and stabilizing the plasmalemmal expression of LRRC8A protein.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kristof Kadar, Viktoria Juhasz, Anna Foldes, Robert Racz, Yan Zhang, Heike Lochli, Erzsebet Kato, Laszlo Koles, Martin C. Steward, Pamela DenBesten, Gabor Varga, Akos Zsembery
Summary: TRPM7 channels are crucial for cellular Ca2+, Zn2+, and Mg2+ homeostasis, particularly in the mineralization of dental enamel. They act as potential modulators of Orai-dependent Ca2+ uptake and pH-sensitive independent Ca2+ entry pathways during amelogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Rikki Chokshi, Orville Bennett, Tetyana Zhelay, J. Ashot Kozak
Summary: NSAIDs are commonly used for pain relief and inflammation, primarily by inhibiting COX enzymes. They have also been found to affect TRPM7 channel activity, leading to intracellular acidification and reduced cell viability.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Francisco J. Rios, Zhi-Guo Zou, Adam P. Harvey, Katie Y. Harvey, Livia L. Camargo, Karla B. Neves, Sarah E. F. Nichol, Rheure Alves-Lopes, Alexius Cheah, Maram Zahraa, Alexey G. Ryazanov, Lillia Ryazanova, Thomas Gudermann, Vladimir Chubanov, Augusto C. Montezano, Rhian M. Touyz
Summary: TRPM7 deficiency may be an important mechanism underlying cardiovascular and renal diseases caused by hyperaldosteronism, and it is associated with hypomagnesemia.
COMMUNICATIONS BIOLOGY
(2022)
Article
Plant Sciences
Xinmin Lv, Shilian Huang, Jing Wang, Dongmei Han, Jianguang Li, Dongliang Guo, Haifeng Zhu
Summary: This study analyzed and located the members of the magnesium transporter (MGT) family in longan fruit. The results showed that DlMGTs family members are distributed in the cell membrane, chloroplast, and nucleus. The study also found an interaction between DlMGT1 and the transcription factor DlGATA16, suggesting a potential role of DlGATA16 in regulating the transcriptional activity of DlMGT1.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Hari Krishna Sadhanala, Saurav Aryal, Kusha Sharma, Ziv Orpaz, Shulamit Michaeli, Aharon Gedanken
Summary: In this study, photoluminescent N-doped carbon dots were synthesized and used for the selective detection and imaging of intracellular magnesium ions. This is the first report of using carbon dots for sensing and imaging of magnesium ions in cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Cardiac & Cardiovascular Systems
Qing Liu, Suyao Li, Yuran Qiu, Jiayu Zhang, Francisco J. Rios, Zhiguo Zou, Rhian M. Touyz
Summary: Receptor tyrosine kinases are membrane spanning receptors that play important roles in cancer development. Targeted therapies against these receptors have been widely used in cancer treatment, but they can also cause cardiovascular toxicity. This review discusses the implications of tyrosine kinase inhibitors in cardiovascular toxicities and explores the potential role of TRPM7/Mg2+ in mediating these effects.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Inga Andriule, Dalia Pangonyte, Mante Almanaityte, Vaiva Patamsyte, Milda Kupryte, Dainius Karciauskas, Kanigula Mubagwa, Regina Macianskiene
Summary: The study demonstrates the presence and co-expression of TRPM6 and TRPM7 in cardiomyocytes from all chamber walls of the human heart, with increased expression in tissues from patients with ischemic heart disease. This suggests that these channels-enzymes may play a role in the pathophysiology of the disease.
SCIENTIFIC REPORTS
(2021)
Article
Spectroscopy
Xianfa Zhang, Shuaibing Yu, Xuliang Pang, Xiaochen Ren, Bo Zhang, Jinming Kong, Lianzhi Li
Summary: A new Schiff base multiple correspondence fluorescent probe, (E)-2-(2-hydroxybenzylidene) hydrazine-1-carboxamid (L), was synthesized for selective sensing of Cu2+ and Mg2+ ions. L showed excellent selectivity and high sensitivity toward Cu2+ in turn off mode with a detection limit of 40.5 nM in 10 mM, pH = 7.0 PBS buffer. On the other hand, L exhibited highly selective and sensitive fluorescence sensing ability for Mg2+ in turn on mode with a detection limit of 9.5 nM when acetonitrile was used as the solvent. The binding modes of L with Cu2+ and Mg2+ were investigated, and satisfactory results were obtained when L was used to detect Cu2+ and Mg2+ in real water samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Materials Science, Multidisciplinary
Hari Krishna Sadhanala, Sudhakar Pagidi, Aharon Gedanken
Summary: The study successfully synthesized highly fluorescent boron-doped carbon dots (BCDs) using a simple method and reported their application in the highly selective and sensitive detection of Mg2+ ions for the first time. BCDs exhibited a high quantum yield and could be effectively reused, showing potential for simple and cost-effective detection of intracellular Mg2+ ions.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Neurosciences
Jie Xu, Yan-Tian Lv, Xiao-Yun Zhao, Jing-Jing Wang, Zhong-Shan Shen, Jian Li, Fei-Fei Zhang, Jing Liu, Xiao-Hui Wang, Yun Xu, Qi Geng, Yi-Tong Ding, Jing-Jing Xu, Meng-Jiao Tan, Zhi-Xiao Li, Ran Wang, Jian Chen, Wen Sun, Meng Cui, Diomedes E. Logothetis, Jun-li Cao, Qiong-Yao Tang, Zhe Zhang
Summary: In this study, two potential sodium-sensitive sites were identified in the C-terminal domain of the rat Slack channel, and R379 was determined to be a chloride interaction site. These findings contribute to a better understanding of the sodium gating mechanism and the regulation of Slack channel function.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Aleksandra S. Anisimova, Mark B. Meerson, Maxim Gerashchenko, Ivan Kulakovskiy, Sergey E. Dmitriev, Vadim N. Gladyshev
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Biochemistry & Molecular Biology
Ivan I. Sorokin, Konstantin S. Vassilenko, Ilya M. Terenin, Natalia O. Kalinina, Vadim I. Agol, Sergey E. Dmitriev
Summary: Viruses utilize various strategies to compete for the translation machinery within infected cells, such as bypassing the need for m(7)G-cap, utilizing internal ribosome entry sites, and perturbing conventional ribosomal scanning. Understanding these unconventional mechanisms of viral mRNA translation initiation is crucial for developing new approaches to antiviral therapy.
BIOCHEMISTRY-MOSCOW
(2021)
Article
Biochemistry & Molecular Biology
Elena M. Maksimova, Alexey P. Korepanov, Olesya V. Kravchenko, Timur N. Baymukhametov, Alexander G. Myasnikov, Konstantin S. Vassilenko, Zhanna A. Afonina, Elena A. Stolboushkina
Summary: Ribosome biogenesis is a complex and coordinated process, with cryo-electron microscopy revealing the role of the RbfA factor in the assembly of 30S ribosomal subunits, particularly in the formation of the central pseudoknot and positioning of helix 44.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kseniya A. Lashkevich, Sergey E. Dmitriev
Summary: The spatial organization of protein biosynthesis in eukaryotic cells is influenced by various factors, including the endoplasmic reticulum, mitochondria, and other organelles. Localized translation can be determined by signals in the synthesized protein, mRNA sequences, and RNA-binding proteins. Viral infections can also impact protein synthesis regulation and localization.
Article
Biochemistry & Molecular Biology
Asya G. Davidian, Alexander G. Dyomin, Svetlana A. Galkina, Nadezhda E. Makarova, Sergey E. Dmitriev, Elena R. Gaginskaya
Summary: The analysis of turtle rDNA sequences revealed a specific insertion of NOR-5S rRNA gene with antisense orientation. This insertion exhibited specific expression in oocytes, playing a potential role in oocyte-specific translation. The NOR-5S rRNA predominance in ribosomes during vitellogenic oocytes suggests a distinct regulation of ribosomal RNA expression in this process.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Artyom A. Egorov, Alexander Alexandrov, Valery N. Urakov, Desislava S. Makeeva, Roman O. Edakin, Artem S. Kushchenko, Vadim N. Gladyshev, Ivan Kulakovskiy, Sergey E. Dmitriev
Summary: The use of the standard G418-resistance cassette for producing knockout mutants in Saccharomyces cerevisiae has potential side effects on neighboring gene expression, affecting transcription efficiency and mRNA translation. Knockout can lead to changes in the transcription start site or alternative polyadenylation signals of neighboring genes, influencing their 5' and 3' UTRs. These events may explain the phenomenon of false genetic interactions due to neighboring gene effects.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Inna A. Nikonorova, Juan Wang, Alexander L. Cope, Peter E. Tilton, Kaiden M. Power, Jonathon D. Walsh, Jyothi S. Akella, Amber R. Krauchunas, Premal Shah, Maureen M. Barr
Summary: Extracellular vesicles (EVs) can mediate intercellular communication by carrying protein and RNA cargo. C. elegans is a tractable animal system for studying and identifying EV cargo. The study found that EVs in C. elegans also carry RNA, suggesting a potential role for EVs in extracellular RNA-based communication.
Article
Virology
Anna Shishova, Ilya Dyugay, Ksenia Fominykh, Victoria Baryshnikova, Alena Dereventsova, Yuriy Turchenko, Anna A. Slavokhotova, Yury Ivin, Sergey E. Dmitriev, Anatoly Gmyl
Summary: The study demonstrates that enteroviruses actively modulate the host defensive pathway by inducing phosphorylation and proteolytic cleavage of the ER stress sensor Ire1, as well as down-regulating its splicing activity. Inactivation of Ire1 could be a novel mode of the UPR manipulation employed by viruses to modify the ER stress response in infected cells.
Article
Cell Biology
Desislava L. Makeeva, Claire V. Riggs, Anton A. Burakov, Pavel S. Ivanov, Artem A. Kushchenko, Dmitri I. Bykov, Vladimir S. Popenko, Vladimir V. Prassolov, Pavel V. E. Ivanov, Sergey Dmitriev
Summary: Upon oxidative stress, mammalian cells reprogram their translation and form stress granules (SGs). Arsenite-induced stress increases stop-codon readthrough rate and translation reinitiation levels on uORF-containing and bicistronic mRNAs. Translation termination factors, ribosome recycling, and translation reinitiation factors are recruited to SGs during arsenite treatment. This spatial redistribution of translation factors may contribute to mRNA translation resumption and suggest the presence of post-termination, recycling, or reinitiation complexes in SGs.
Article
Biochemistry & Molecular Biology
Aleksandra S. Anisimova, Natalia M. Kolyupanova, Nadezhda E. Makarova, Artyom A. Egorov, Ivan V. Kulakovskiy, Sergey E. Dmitriev
Summary: Through analyzing transcriptomic data, we found abundance differences of translation factors and aminoacyl-tRNA synthetases encoding genes in different human tissues. We identified several novel tissue-specific translation-related genes and confirmed their protein products. We also observed widespread tissue-specific variance in the composition of translation initiation, elongation, and termination complexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Bohan Zhang, David E. Lee, Alexandre Trapp, Alexander Tyshkovskiy, Ake T. Lu, Akshay Bareja, Csaba Kerepesi, Lauren K. McKay, Anastasia V. Shindyapina, Sergey E. Dmitriev, Gurpreet S. Baht, Steve Horvath, Vadim N. Gladyshev, James P. White
Summary: Heterochronic parabiosis (HPB) is a technique that has rejuvenation effects on mouse tissues, reducing biological age and improving long-term health. In this study, mice exposed to young circulation through HPB showed reduced epigenetic aging, and this effect lasted even after the removal of youthful circulation. The transcriptomic and epigenomic profiles of these mice indicated a global rejuvenation effect. Furthermore, gene expression changes in the old HPB mice resembled those seen in interventions that extend lifespan. Overall, HPB results in lasting changes in epigenetic and transcriptomic profiles, leading to an extension of lifespan and health span.
Article
Cell Biology
Rui Ribeiro, Joana C. Macedo, Madalena Costa, Vladimir Ustiyan, Anastasia Shindyapina, Alexander Tyshkovskiy, Rita N. Gomes, Jose Pedro Castro, Tanya Kalin, Francisco Vasques-Novoa, Diana S. Nascimento, Sergey E. Dmitriev, Vadim N. Gladyshev, Vladimir V. Kalinichenko, Elsa Logarinho
Summary: The FOXM1 gene plays a crucial role in maintaining cellular homeostasis and has the potential to delay cellular and organismal aging, thereby mitigating aging-related pathologies.
Meeting Abstract
Biochemistry & Molecular Biology
D. O. Vladimirov, K. A. Lashkevich, S. E. Dmitriev
Meeting Abstract
Biochemistry & Molecular Biology
A. Anisimova, N. Kolyupanova, N. Makarova, A. Egorov, I. Kulakovskiy, S. Dmitriev
