期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 10, 页码 6616-6629出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku287
关键词
-
资金
- La Foundation pour la Recherche Medicale (Equipes FRM)
- Agence National dela Recherche [ANR-13-BSV8-0001-01]
- Fondation ARC pour la Research sur le Cancer post-doctoral fellowship
- FRM PhD fellowship
- Agence Nationale de la Recherche (ANR) [ANR-13-BSV8-0001] Funding Source: Agence Nationale de la Recherche (ANR)
Post-transcriptional pseudouridylation and 2'-Omethylation of splicesomal small nuclear ribonucleic acids (snRNAs) is mediated by box H/ACA and box C/D small Cajal body (CB)-specific ribonucleoproteins (scaRNPs), respectively. The WD-repeat protein 79 (WDR79) has been proposed to interact with both classes of modification scaRNPs and target them into the CB. The box H/ACA scaRNAs carry the common CAB box motif (consensus, ugAG) that is required for both WDR79 binding and CB-specific accumulation. Thus far, no cis-acting CB-localization element has been reported for vertebrate box C/D scaRNAs. In this study, systematic mutational analysis of the human U90 and another newly identified box C/D scaRNA, mgU2-47, demonstrated that the CB-specific accumulation of vertebrate intron-encoded box C/D scaRNAs relies on GU- or UG-dominated dinucleotide repeat sequences which are predicted to form the terminal stem-loop of the RNA apical hairpin. While the loop nucleotides are unimportant, the adjacent terminal helix that is composed mostly of consecutive G.U and U.G wobble base-pairs is essential for CB-specific localization of box C/D scaRNAs. Co-immunoprecipitation experiments confirmed that the newly identified CB localization element, called the G.U/U.G wobble stem, is crucial for in vivo association of box C/D scaRNPs with WDR79.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据