Article
Cell Biology
Jie-Ning Li, Hui-Lung Sun, Ming-Yang Wang, Pai-Sheng Chen
Summary: MicroRNAs (miRNAs) are small non-coding RNAs that play a role in regulating pathophysiological processes by suppressing target gene expression post-transcriptionally. The function of miRNAs is regulated by a conserved biogenesis machinery involving processing steps and the formation of a miRNA-induced silencing complex (miRISC). This study discovered a novel regulatory mechanism involving the post-translational modification of AGO2 by E-cadherin, leading to enhanced miRISC activity on gene silencing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Cuili Liang, Yitong Yan, Yingchao Tan, Xue Yang, Jie Cao, Chaorong Tang, Kaiye Liu
Summary: This study identified miRNAs and their targets in two dandelion species and revealed their potential role in regulating macromolecular biosynthesis and metabolism for natural rubber production in latex.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tianxiao Huang, Yan Li, Wei Wang, Le Xu, Jingrui Li, Yijun Qi
Summary: This study reannotated lmiRNAs and their targets in rice, revealing that most lmiRNAs are derived from MITEs and suggesting co-evolution between lmiRNAs and their targets through MITE amplification. The study also found dynamic changes of lmiRNAs under stress conditions and enrichment of stress-responsive genes among lmiRNA targets, indicating the widespread involvement of lmiRNAs in plant stress responses. Furthermore, the study constructed the evolutionary histories of lmiRNAs and their targets, highlighting the importance of MITEs in the origin of lmiRNAs and suggesting a role for the evolution of lmiRNA-target regulatory modules in rice adaptation to environmental changes.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jesus Garcia-Castillo, Francisca Alcaraz-Perez, Elena Martinez-Balsalobre, Diana Garcia-Moreno, Marlies P. Rossmann, Miriam Fernandez-Lajarin, Manuel Bernabe-Garcia, Ana B. Perez-Oliva, Virginia C. Rodriguez-Cortez, Clara Bueno, Isaac Adatto, Suneet Agarwal, Pablo Menendez, Leonard Zon, Victoriano Mulero, Maria L. Cayuela
Summary: Dyskeratosis congenita (DC) is a rare genetic syndrome caused by mutations in telomerase or telomeric proteins. Research shows that zebrafish terc plays a crucial role in regulating the expression of master myeloid genes, while mutations found in DC patients affect this process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Qian Li, Nadil Shah, Xueqing Zhou, Huiying Wang, Wenlin Yu, Jiajie Luo, Yajun Liu, Genze Li, Chao Liu, Chunyu Zhang, Peng Chen
Summary: The study revealed that miRNAs play different roles in response to Clubroot disease infection in resistant and susceptible plants, potentially participating in the resistance mechanisms through regulating growth, cell death, and metabolic pathways.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yunchen Luo, Yongyan Wang, Xin Li, Xiaohan Yang, Huiru Bai, Xiaoqin Liao, Xuanling Luo, Fan Zhang, Lei Zhang, Qinglin Liu
Summary: Cold stress affects the growth and development of plants, and this study reveals the important regulatory roles of MYB transcription factors and histone H3K4me3 transferase ATXs in plant resistance to low temperatures. The study demonstrates that DgMYB and DgATX interact with each other and regulate H3K4me3 levels at DgPOD, leading to increased cold tolerance in chrysanthemums.
Article
Plant Sciences
Mohandas Snigdha, Duraisamy Prasath
Summary: This study analyzed the miRNAs and their target genes in ginger and mango ginger in response to R. solanacearum, finding higher enrichment of miRTGs in mango ginger than in ginger. Among the DEmiRTGs, 2105 were common in both ginger and mango ginger, while 2337 were unique to mango ginger, including 62 defense-related and upregulated miRTGs. Several key candidate miRNA/miRTGs pairs were identified in response to R. solanacearum infection in ginger, providing insights into the miRNA regulatory network in ginger and mango ginger in response to bacterial wilt.
Article
Immunology
Ibrahim A. Akkouh, Timothy Hughes, Vidar M. Steen, Joel C. Glover, Ole A. Andreassen, Srdjan Djurovic, Attila Szabo
Summary: Despite the high heritability of schizophrenia, the detailed pathophysiology and etiology are still unknown. Recent findings suggest that abnormal inflammatory regulation and miRNAs are involved. Comparative analysis of miRNome in SCZ astrocytes revealed four differentially expressed miRNAs with lower baseline expression compared to healthy controls. Peripheral blood analysis also showed significantly lower expression of specific target genes in SCZ patients, further enhancing the understanding of SCZ pathobiology.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Endocrinology & Metabolism
Jose M. Murrieta-Coxca, Emanuel Barth, Paulina Fuentes-Zacarias, Ruby N. Gutierrez-Samudio, Tanja Groten, Alexandra Gellhaus, Angela Koeninger, Manja Marz, Udo R. Markert, Diana M. Morales-Prieto
Summary: Placenta accreta spectrum (PAS) is a group of pathological conditions characterized by abnormal attachment of the placenta to the uterus wall, and it is a major cause of maternal morbidity and mortality worldwide. In this study, altered miRNA expression, particularly in placenta-specific miRNA clusters, was identified in placenta accreta tissue compared to normal placental tissues. Dysregulated pathways associated with cell cycle regulation, inflammation, and invasion were also observed. The study provides miRNA:mRNA associations that could contribute to our understanding of PAS development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Vrantika Chaudhary, Sumit Jangra, Neelam R. Yadav
Summary: Cluster bean, a drought-tolerant crop with high protein and gum content, was found to have multiple miRNAs and their targets associated with metabolism, biological processes, and stress responses. This study provides valuable information for the identification of miRNAs in cluster bean.
FRONTIERS IN GENETICS
(2022)
Article
Biology
Jenifer Mallavarpu Ambrose, Daniel Alex Anand, Malathi Kullappan, Sardar Hussain, Kavin Mozhi James, Radhika Nalinakumari Sreekandan, Sumetha Suga Deiva Suga, Devakumar Kamaraj, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan
Summary: Understanding the genetic differences between humans and chimpanzees is important for biomedical research and the development of new treatments and diagnostic tools. This study compared and annotated miRNAs and gene regulatory mechanisms in the intronic regions of the PHEX gene on the human X chromosome in both species. The results identified common miRNAs and predicted their regulation of cancer-related genes. Further experimental validation is needed before these genes can be used as diagnostic markers and drug targets.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yanbo Chen, Bingbing Liao, Xiaohui Lin, Qishan Luo, Xuanyan Huang, Xiaojing Wang, Qinli Shan, Yaqin Wang
Summary: MiRNAs play a role in regulating the growth and development of gerbera, particularly in ray floret growth and anthocyanin accumulation. Small RNA sequencing provides valuable insights into the mechanisms involved in these processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carlos Lopez-Ortiz, Yadira Pena-Garcia, Menuka Bhandari, Venkata Lakshmi Abburi, Purushothaman Natarajan, John Stommel, Padma Nimmakayala, Umesh K. Reddy
Summary: This study analyzed miRNA contents in cultivated and wild pepper species, revealing differentially expressed miRNAs across species and tissues. Key pathways regulated by miRNAs during pepper fruit ripening include amino acids, sugar and nucleotide metabolism, and starch and sucrose metabolism. Predicted miRNA-target gene interactions provide insights into the mechanisms regulating flowering time and fruit development in Capsicum species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anil Kumar Vijjamarri, Neha Gupta, Chisom Onu, Xiao Niu, Fan Zhang, Rakesh Kumar, Zhenguo Lin, Miriam L. Greenberg, Alan G. Hinnebusch
Summary: We investigated the roles of Pat1 and Dhh1 in regulating mRNA translation and abundance in yeast cells under nutrient-replete conditions. Our findings suggest that Pat1 and Dhh1 cooperatively repress the expression of numerous non-ESR transcripts by reducing decapping and transcription in wild-type cells. Furthermore, we observed impaired mRNA turnover in mutants lacking Dhh1 or Pat1, leading to derepression of these transcripts. Pat1 and Dhh1 also contribute to translational repression and decreased protein production for multiple pathways involved in cell adhesion, nitrogen metabolism, respiration, and autophagy. These findings highlight the post-transcriptional regulatory functions of Pat1 and Dhh1 during nutrient limitation.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Bianca Enaru, Georgiana Dretcanu, Teodora Daria Pop, Andreea Stanila, Zorita Diaconeasa
Summary: Anthocyanins are natural pigments with important functions in nature, as well as potential health benefits such as antioxidant, anticancer, anti-inflammatory and antimicrobial properties. However, their low stability is a major drawback, influenced by various factors such as pH, light, temperature, co-pigmentation, sulfites, ascorbic acid, oxygen and enzymes. Understanding the impact of these factors on anthocyanin stability is crucial to maximize their health benefits and minimize negative effects.
Article
Biochemistry & Molecular Biology
Maninjay K. Atianand, Wenqian Hu, Ansuman T. Satpathy, Ying Shen, Emiliano P. Ricci, Juan R. Alvarez-Dominguez, Ankit Bhatta, Stefan A. Schattgen, Jason D. McGowan, Juliana Blin, Joerg E. Braun, Pallavi Gandhi, Melissa J. Moore, Howard Y. Chang, Harvey F. Lodish, Daniel R. Caffrey, Katherine A. Fitzgerald
Article
Biology
Joerg E. Braun, Larry J. Friedman, Jeff Gelles, Melissa J. Moore
Article
Biochemistry & Molecular Biology
Ying Chen, Andreas Boland, Duygu Kuzuoglu-Ozturk, Praveen Bawankar, Belinda Loh, Chung-Te Chang, Oliver Weichenrieder, Elisa Izaurralde
Article
Biochemistry & Molecular Biology
Stefanie Jonas, Mary Christie, Daniel Peter, Dipankar Bhandari, Belinda Loh, Eric Huntzinger, Oliver Weichenrieder, Elisa Izaurralde
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Chung-Te Chang, Natalia Bercovich, Belinda Loh, Stefanie Jonas, Elisa Izaurralde
NUCLEIC ACIDS RESEARCH
(2014)
Article
Oncology
Bastian J. Schmied, Fabian Riegg, Latifa Zekri, Ludger Grosse-Hovest, Hans-Joerg Buehring, Gundram Jung, Helmut R. Salih
Article
Oncology
Bastian J. Schmied, Martina S. Lutz, Fabian Riegg, Latifa Zekri, Jonas S. Heitmann, Hans-Joerg Buehring, Gundram Jung, Helmut R. Salih
Article
Oncology
Joseph Kauer, Sebastian Hoerner, Lukas Osburg, Stefanie Mueller, Melanie Maerklin, Jonas S. Heitmann, Latifa Zekri, Hans-Georg Rammensee, Helmut R. Salih, Gundram Jung
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Article
Medicine, Research & Experimental
Latifa Zekri, Fabian Vogt, Lukas Osburg, Stefanie Mueller, Joseph Kauer, Timo Manz, Martin Pfluegler, Andreas Maurer, Jonas S. Heitmann, Ilona Hagelstein, Melanie Maerklin, Sebastian Hoerner, Tilmann Todenhoefer, Carsten Calaminus, Arnulf Stenzl, Bernd Pichler, Christian laFougere, Marc A. Schneider, Hans-Georg Rammensee, Lars Zender, Bence Sipos, Helmut R. Salih, Gundram Jung
Summary: The PSMA antibody 10B3 exhibits superior dual reactivity on prostate carcinoma and squamous cell carcinoma tissues, and is used to construct T-cell recruiting bispecific antibodies IgGsc, which show better efficacy in eliminating tumors. Treatment with IgGsc in three patients with metastasized prostate carcinoma resulted in significant T-cell activation and rapid reduction of elevated PSA levels.
EMBO MOLECULAR MEDICINE
(2021)
Article
Oncology
Joseph Kauer, Fabian Vogt, Ilona Hagelstein, Sebastian Hoerner, Melanie Maerklin, Stefanie Maurer, Helmut R. Salih, Gundram Jung, Latifa Zekri
Summary: Bispecific antibodies are effective in activating T cells against cancer, but can also cause cytokine release syndrome. Blocking the adhesion molecule CD18 can reduce unwanted T cell activation without affecting the anti-tumor effect. This opens up the possibility of preventing off-target T cell activation and allowing for effective doses of bispecific antibodies.
Article
Oncology
Martina Svenja Lutz, Boris Klimovich, Stefanie Maurer, Jonas S. Heitmann, Melanie Maerklin, Latifa Zekri, Gundram Jung, Helmut R. Salih, Clemens Hinterleitner
Summary: This study found that platelet activation reduces the efficacy of T cell-recruiting antibodies, and the effect can be restored by blocking the transforming growth factor beta (TGF-beta) axis.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Naveen K. Mehta, Martin Pfluegler, Kristan Meetze, Bochong Li, Isabelle Sindel, Fabian Vogt, Melanie Marklin, Jonas S. Heitmann, Joseph Kauer, Lukas Osburg, Latifa Zekri, Hans-Joerg Buehring, Stefanie Mueller, Sebastian Hoerner, Patrick A. Baeuerle, Jennifer S. Michaelson, Gundram Jung, Helmut R. Salih
Summary: Immunotherapeutic strategies using CAR-T cells and bsAbs have achieved remarkable clinical success in lymphoid malignancies but not in acute myeloid leukemia (AML). This study developed a bsAb, CLN-049, targeting FLT3 and demonstrated its favorable efficacy and safety profile in preclinical models, indicating its potential as a treatment option for AML.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Immunology
Ilona Hagelstein, Monika Engel, Clemens Hinterleitner, Timo Manz, Melanie Maerklin, Gundram Jung, Helmut R. Salih, Latifa Zekri
Summary: This study found that B7-H3 is highly expressed in bone and soft-tissue sarcomas, and proposed the use of a B7-H3 monoclonal antibody with a variant Fc part to enhance NK cell cytotoxicity. The results demonstrate the potential of this antibody to effectively kill various types of sarcoma cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Sebastian Horner, Moustafa Moustafa-Oglou, Karin Teppert, Ilona Hagelstein, Joseph Kauer, Martin Pfluegler, Kristina Neumann, Hans-Georg Rammensee, Thomas Metz, Andreas Herrmann, Helmut R. Salih, Gundram Jung, Latifa Zekri
Summary: This article presents a novel approach to selectively induce apoptosis in lymphoma cells and autoreactive B cells using bispecific antibodies. The developed IgG-based bispecific antibodies show efficient depletion of malignant and autoreactive B cells in vitro and in vivo.
Article
Biotechnology & Applied Microbiology
Latifa Zekri, Martina Lutz, Nisha Prakash, Timo Manz, Boris Klimovich, Stefanie Mueller, Sebastian Hoerner, Ilona Hagelstein, Monika Engel, Anna Chashchina, Martin P. fl Uegler, Jonas S. Heitmann, Gundram Jung, Helmut R. Salih
Summary: T cell-based immunotherapy has been revolutionary in oncological treatment, but its effectiveness in gastrointestinal cancers is limited. The overexpression of B7-H3 in colorectal cancer (CRC) provides a potential target for therapeutic treatment. A panel of bispecific antibodies targeting B7-H3 and CD3 was generated, and a lead compound (CC-3) showed promising results in vitro and in vivo, demonstrating strong antitumor activity. CC-3 is currently undergoing production to enable evaluation in a clinical study for CRC treatment.