Article
Multidisciplinary Sciences
Salim T. Islam, Nicolas Y. Jolivet, Clemence Cuzin, Akeisha M. Belgrave, Laetitia My, Betty Fleuchot, Laura M. Faure, Utkarsha Mahanta, Ahmad A. Kezzo, Fares Saidi, Gaurav Sharma, Jean -Bernard Fiche, Benjamin P. Bratton, Julien Herrou, Marcelo Nollmann, Joshua W. Shaevitz, Eric Durand, Tam Mignot
Summary: The predatory deltaproteobacterium Myxococcus xanthus utilizes a helically-trafficked motor at bacterial focal-adhesion (bFA) sites for gliding motility. The von Willebrand A domain-containing outer-membrane (OM) lipoprotein CglB is identified as an essential adhesin of the gliding transducer (Glt) machinery at bFAs. The Glt OM platform mediates cell-surface accessibility and retention of CglB, promoting regulated surface exposure of CglB at bFAs and transduction of contractile forces to the substratum.
Article
Nutrition & Dietetics
Paulina Wawrzyniak, Barbara Hubeli, Marcin Wawrzyniak, Nazek Noureddine, Asa Walberg, Sylvie Scharl, Matthias Turina, Michael Scharl, Michael Zaugg, Stefanie D. Kramer, Gerhard Rogler, Martin Hersberger
Summary: This study compares the effects of two commonly used lipid emulsions, Omegaven (n-3-rich) and Intralipid (n-6-rich), on inflammation in human peripheral blood mononuclear cells (PBMCs). The results show that Omegaven has anti-inflammatory effects and increases the number of regulatory T cells, making it suitable for patients with inflammatory conditions. However, it may not be suitable for patients with cancer.
CLINICAL NUTRITION
(2023)
Article
Oncology
Elom K. Aglago, Andre Kim, Yi Lin, Conghui Qu, Marina Evangelou, Yu Ren, John Morrison, Demetrius Albanes, Volker Arndt, Elizabeth L. Barry, James W. Baurley, Sonja Berndt, Stephanie A. Bien, D. Timothy Bishop, Emmanouil Bouras, Hermann Brenner, Daniel D. Buchanan, Ari Budiarto, Robert Carreras-Torres, Graham Casey, Tjeng Wawan Cenggoro, Andrew T. Chen, Jenny Chang-Claude, Xuechen Chen, David Conti, Matthew Devall, Virginia Diez-Obrero, Niki Dimou, David Drew, Jane C. Figueiredo, Steven Gallinger, Graham G. Giles, Stephen B. Gruber, Andrea Gsur, Marc J. Gunter, Heather Hampel, Sophia Harlid, Akihisa Hidaka, Tabitha A. Harrison, Michael Hoffmeister, Jeroen R. Huyghe, Mark A. Jenkins, Kristina Jordahl, Amit D. Joshi, Eric S. Kawaguchi, Temitope O. Keku, Anshul Kundaje, Susanna C. Larsson, Loic Le Marchand, Juan Pablo Lewinger, Li Li, Brigid M. Lynch, Bharuno Mahesworo, Marko Mandic, Mireia Obon-Santacana, Victor Morento, Neil Murphy, Hongmei Men, Rami Nassir, Polly A. Newcomb, Shuji Ogino, Jennifer Ose, Rish K. Pai, Julie R. Palmer, Nikos Papadimitriou, Bens Pardamean, Anita R. Peoples, Elizabeth A. Platz, John D. Potter, Ross L. Prentice, Gad Rennert, Edward Ruiz-Narvaez, Lori C. Sakoda, Peter C. Scacheri, Stephanie L. Schmit, Robert E. Schoen, Anna Shcherbina, Martha L. Slattery, Mariana C. Stern, Yu-Ru Su, Catherine M. Tangen, Stephen N. Thibodeau, Duncan C. Thomas, Yu Tian, Cornelia M. Ulrich, Franzel J. B. van Duijnhoven, Bethany Van Guelpen, Kala Visvanathan, Pavel Vodicka, Jun Wang, Emily White, Alicja Wolk, Michael O. Woods, Anna H. Wu, Natalia Zemlianskaia, Li Hsu, W. James Gauderman, Ulrike Peters, Konstantinos K. Tsilidis, Peter T. Campbell
Summary: Colorectal cancer risk is influenced by genetic, environmental, and lifestyle factors. This study identified a gene-environment interaction between body mass index (BMI) and a specific gene that increases the risk of colorectal cancer.
Article
Biochemistry & Molecular Biology
Morgan A. Gingerich, Jie Zhu, Biaoxin Chai, Michael P. Vincent, Nuli Xie, Vaibhav Sidarala, Nicholas A. Kotov, Debashish Sahu, Daniel J. Klionsky, Santiago Schnell, Scott A. Soleimanpour
Summary: CLEC16A is an E3 ubiquitin ligase that plays a crucial role in regulating mitochondrial quality control through mitophagy and is associated with various human diseases. A recent study discovered an internal intrinsically disordered protein region (IDPR) within CLEC16A that is essential for its function and turnover. This IDPR is targeted by RNF41 to promote CLEC16A degradation and destabilize the CLEC16A-RNF41-USP8 complex. These findings highlight the importance of the IDPR in controlling the regulatory balance between CLEC16A and RNF41, which could be therapeutically targeted to improve mitochondrial health in disease.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Michele F. Caliandro, Felix Schmalbein, Luca Matteo Todesca, Matthias Moergelin, Maryam Rezaei, Juliane Meissner, Isabel Siepe, Julius Grosche, Albrecht Schwab, Johannes A. Eble
Summary: Integrin-mediated cell contacts with the extracellular matrix (ECM) play essential roles in cellular adhesion, force transmission, and migration. The binding activities of integrins and their cellular functions are regulated by various effectors, such as divalent cations and redox-active compounds. This study investigates the interplay between the Ca2+ binding site and redox-regulation within the hinge region of the integrin alpha 7 subunit, revealing their mutual dependence in adhesion strength and cell migration. Furthermore, protein-chemical analyses suggest that the activation of integrin via the alpha hinge is primed by the formation of cysteine pair-based crosslinkage, followed by Ca2+ complexation, resulting in altered cell functions mediated by integrin and ECM ligand binding.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Magda Rybicka, Eloi R. Verrier, Thomas F. Baumert, Krzysztof Piotr Bielawski
Summary: This study included 284 patients with chronic hepatitis B virus infection and found that certain genetic variations in DIO2, PPARG, GADD45A, and ATF3 were associated with liver fibrosis and cirrhosis in this population. Additionally, a variation in DIO2 was associated with hepatocellular carcinoma.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Vanessa Meier-Stephenson, Maulik D. Badmalia, Tyler Mrozowich, Keith C. K. Lau, Sarah K. Schultz, Darren L. Gemmill, Carla Osiowy, Guido van Marle, Carla S. Coffin, Trushar R. Patel
Summary: A unique G-quadruplex structure in the pre-core promoter region of the HBV genome has been discovered in this study, which may lead to the identification of novel anti-HBV cccDNA targets and enhance the understanding of the HBV pre-core region.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Seth Scott, You Li, Oya Bermek, Jack D. Griffith, Stanley M. Lemon, Kyung H. Choi
Summary: Hepatitis C virus replication relies on the interaction between cellular factors miR-122 and PCBP2. These factors compete for binding to specific sites on the RNA genome, regulating the viral replication cycle. MiR-122 disrupts genome circularization and promotes recruitment of the viral polymerase by competing with PCBP2 for binding.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Infectious Diseases
Nazim Hussain, Simona Bungau, Tapan Behl, Aayush Sehgal, Maheen Mahmood, Sadia Manzoor, Muhammad Abdul Qayyum, Muhammad Imran Khan, Lavinia Cristina Moleriu, Muhammad Bilal
Summary: This study investigated 4177 HCV-infected patients presenting to the diagnostic facility of the Centre for Applied Molecular Biology, University of Punjab, Lahore between 2016 and 2019, revealing no significant differences in the prevalence of various genotypes between genders in the adult population. Genotype 3a was found to be the most common, while 28.23% of patients had untypable genomes and 1.22% of samples were non-detectable as viremic.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Jesus Castillo-Martinez, Lixin Fan, Mateusz P. Szewczyk, Yun-Xing Wang, Jose Gallego
Summary: Subdomain 5BSL3.2 of hepatitis C virus RNA plays a crucial role in connecting different regions of the viral genome and regulating viral translation and replication. The structural models of this subdomain and its complex with 3' X region have been determined, revealing their unique shapes and their impact on viral polymerase binding and RNA synthesis. The conservation of similar motifs in flaviviruses suggests a common regulatory mechanism within the Flaviviridae family.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Angelina T. Huynh, Thi-Tina N. Nguyen, Carina A. Villegas, Saira Montemorso, Benjamin Strauss, Richard A. Pearson, Jason G. Graham, Jonathan Oribello, Rohit Suresh, Brooke Lustig, Ningkun Wang
Summary: In this study, a switch-like region was identified in the N-terminal domain of human SIRT1 using a novel computational workflow and biochemical methods. A key residue, M193, essential for allosteric regulation was pinpointed. This method of predicting switch-like regions in allosterically regulated enzymes based on the primary sequence could be an efficient way to identify key residues for therapeutic drug targeting and other applications.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Infectious Diseases
Narges Eslami, Vahdat Poortahmasebi, Javid Sadeghi, Reza Ghotaslou, Bahram Niknafs, Hossein Bannazadeh Baghi, Mahin Ahangar Oskouee
Summary: This study investigated the prevalence of occult hepatitis B virus infection (OBI) in hemodialysis (HD) patients in the East Azerbaijan Province of Iran. The study found a prevalence rate of 4.2% for OBI in HBsAg-negative HD patients in the region. The majority of the samples belonged to genotype D, subtype ayw2, with a few samples showing mutations in the S gene's major hydrophilic region (MHR).
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Junyan Tang, Xianbo Yang, Jose A. Hejase, Mahesh Bohra, Yanyan Zhang, Xiaomin Duan, Dierk Kaller, Wiren D. Becker, Daniel M. Dreps
Summary: This study demonstrates that controlling the length of pin area wiring and other printed circuit board design properties can achieve crosstalk cancellation. Additionally, it shows that crosstalk cancellation or mitigation can be achieved by super positioning crosstalk from different sections. Electromagnetic modelling and signal integrity analysis are used to illustrate these findings.
IEEE 30TH CONFERENCE ON ELECTRICAL PERFORMANCE OF ELECTRONIC PACKAGING AND SYSTEMS (EPEPS 2021)
(2021)
Article
Chemistry, Medicinal
Alfredo Berzal-Herranz, Cristina Romero-Lopez
Review
Biochemistry & Molecular Biology
Sara Ramos-Lorente, Cristina Romero-Lopez, Alfredo Berzal-Herranz
Summary: The genus Flavivirus utilizes RNA-RNA interactions to encode functional information and regulate the production of sfRNAs for maintaining viral fitness. These structural elements play essential regulatory roles in the viral cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Virology
Carlos Garcia-Crespo, Isabel Gallego, Maria Eugenia Soria, Ana Isabel de Avila, Brenda Martinez-Gonzalez, Lucia Vazquez-Sirvent, Rebeca Lobo-Vega, Elena Moreno, Jordi Gomez, Carlos Briones, Josep Gregori, Josep Quer, Esteban Domingo, Celia Perales
Summary: The replication of RNA viruses involves exploring sequence space to adapt to changing environments. Research on hepatitis C virus (HCV) has shown that despite prolonged replication, viral clones continue to expand in sequence space. The study suggests that HCV populations exhibit a permanent state of disequilibrium, potentially facilitating the finding of alternative mutational pathways for antiviral resistance.
Article
Multidisciplinary Sciences
Victor M. Rivilla, Izaskun Jimenez-Serra, Jesus Martin-Pintado, Carlos Briones, Lucas F. Rodriguez-Almeida, Fernando Rico-Villas, Belen Tercero, Shaoshan Zeng, Laura Colzi, Pablo de Vicente, Sergio Martin, Miguel A. Requena-Torres
Summary: Cell membranes, crucial for life, are composed of phospholipids. The presence of ethanolamine in space suggests its potential role in the assembly of primitive membranes on early Earth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Cristina Aira, Jose Ignacio Klett-Mingo, Tamara Ruiz, Ana Garcia-Sacristan, Gerard Eduard Martin-Valls, Enric Mateu, Jaime Gomez-Laguna, Paloma Rueda, Victor Manuel Gonzalez, Maria Jose Rodriguez, Lissette Lopez
Summary: Influenza viruses, classified into different types based on conserved proteins, are significant pathogens for both mammalian and avian species. Swine influenza virus (SIV) causes endemic infections in pigs, leading to economic losses. Current diagnosis methods rely on complex and expensive technologies, but the implementation of aptamers shows promise for improving detection efficiency.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Maria Vergara-Barberan, Maria Jesus Lerma-Garcia, Ernesto F. Simo-Alfonso, Marta Garcia-Hernandez, M. Elena Martin, Ana Garcia-Sacristan, Victor M. Gonzalez, Jose Manuel Herrero-Martinez
Summary: In this study, DNA aptamers with high binding affinity to the major allergen of olive pollen, Ole e 1, were selected using SELEX method. The binding of the aptamers was confirmed by ELONA and aptaprecipitation assays. Aptamer-modified capillary chromatography demonstrated the selective recognition of Ole e 1 against non-target proteins. The stability and reproducibility of the aptamer-modified monolithic column were suitable for practical applications.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Cristina Romero-Lopez, Alfredo Berzal-Herranz, Jose Luis Martinez-Guitarte, Mercedes de la Fuente
Summary: The telomeric transcriptome of Chironomus riparius is involved in thermal stress response, with the CriTER-A variant highly overexpressed during heat shock compared to the less affected CriTER-B variant. The study suggests that CriTER-A folds into two different conformers under temperature conditions, while CriTER-B variant exhibits a single dominant conformer. This temperature-dependent conformational equilibrium of CriTER-A may indicate a potential functional role of the telomeric transcriptome in response to thermal stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Beatriz Torres-Vazquez, Ana Maria de Lucas, Carlos Garcia-Crespo, Juan Antonio Garcia Martin, Adrian Fragoso, Maria Fernandez-Algar, Celia Perales, Esteban Domingo, Miguel Moreno, Carlos Briones, Ana Maria de Lucas, Juan Antonio Garcia-Martin
Summary: Hepatitis C virus (HCV) core protein is a multifunctional protein that can be used for HCV detection and inhibition. In vitro selection of DNA and RNA aptamers against different variants of HCV core protein resulted in the identification of aptamers with high affinity and potential therapeutic applications.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Carlos Cordon-Obras, Claudia Gomez-Linan, Sara Torres-Rusillo, Isabel Vidal-Cobo, Diana Lopez-Farfan, Alicia Barroso-del Jesus, Domingo Rojas-Barros, Mark Carrington, Miguel Navarro
Summary: This study reveals the transcription initiation mechanism of genes in trypanosomes, identifying sequence-specific promoters that regulate the transcription of protein-coding genes, and demonstrates the conservation of this promoter-mediated regulation in eukaryotes.
Review
Chemistry, Medicinal
Cristina Romero-Lopez, Sara Esther Ramos-Lorente, Alfredo Berzal-Herranz
Summary: RNA viruses encode essential information in their genomes using conserved structural elements, establish complex networks of RNA interactions, and targeting these elements has become a promising strategy for antiviral tool development. Understanding viral structure is crucial for identifying potential target sites.
Article
Biochemistry & Molecular Biology
Alfredo Berzal-Herranz, Beatriz Berzal-Herranz, Sara Esther Ramos-Lorente, Cristina Romero-Lopez
Summary: This study provides evidence that West Nile virus promotes a cap-independent translation initiation mechanism in mammalian and insect cells, and suggests that its 3' UTR is involved in fine-tuned translation regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cristina Romero-Lopez, Margarita Roda-Herreros, Beatriz Berzal-Herranz, Sara Esther Ramos-Lorente, Alfredo Berzal-Herranz
Summary: RNA viruses rely on RNA-RNA interactions involving genomic structural elements for viral replication and translation. In this study, intra- and intermolecular RNA-RNA interactions in the 3' UTR of the West Nile virus genome are observed, with intermolecular interactions forming molecular dimers. Functional analysis suggests a relationship between 3' UTR dimerization and viral translation efficiency. These findings imply the existence of a network of RNA-RNA interactions involving 3' UTR structural elements that regulate viral translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ascension Ariza-Mateos, Carlos Briones, Celia Perales, Maria Teresa Bayo-Jimenez, Esteban Domingo, Jordi Gomez
Summary: The entry of a virus into the host cell alters intracellular molecular relationships, leading to the recovery of ancient cellular activities. Viruses can be used as archaeological tools to identify unexpressed activities in non-infected cells, opening new perspectives for molecular biology.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Microbiology
Brenda Martinez-Gonzalez, Isabel Gallego, Josep Gregori, Maria Eugenia Soria, Pilar Somovilla, Ana Isabel de Avila, Carlos Garcia-Crespo, Antoni Duran-Pastor, Carlos Briones, Jordi Gomez, Josep Quer, Esteban Domingo, Celia Perales
Summary: In this study, we report the mild mutagenic activity of sofosbuvir (SOF) for hepatitis C virus (HCV). Serial passages of HCV in the presence of SOF led to increased C & RARR; U transitions in the mutant spectra, indicating its potential as a mild mutagen. This activity was dependent on the fitness of the HCV population and may contribute to its antiviral efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Carlos Garcia-Crespo, Maria Eugenia Soria, Brenda Martinez-Gonzalez, Lucia Vazquez-Sirvent, Rebeca Lobo-Vega, Ana Isabel de Avila, Isabel Gallego, Qian Chen, Damir Garcia-Cehic, Meritxell Llorens-Revull, Carlos Briones, Jordi Gomez, Cristina Ferrer-Orta, Nuria Verdaguer, Josep Gregori, Francisco Rodriguez-Frias, Maria Buti, Juan Ignacio Esteban, Esteban Domingo, Josep Quer, Celia Perales
JOURNAL OF HEPATOLOGY
(2021)