Review
Biochemistry & Molecular Biology
Hideo Tsubouchi
Summary: Homologous recombination (HR) is crucial for meiosis and is induced during meiotic prophase. The Hop2-Mnd1 complex, originally identified in budding yeast, is conserved across species and plays an essential role in meiosis. Accumulating evidence suggests that Hop2-Mnd1 promotes RecA-like recombinases for homology search and strand exchange. This review summarizes studies on the mechanism of the Hop2-Mnd1 complex in promoting HR and beyond.
Article
Cell Biology
Haibo Xie, Xiaosi Wang, Minjun Jin, Lanqin Li, Junwen Zhu, Yunsi Kang, Zhe Chen, Yonghua Sun, Chengtian Zhao
Summary: Meiosis is crucial for evolution and genetic diversity, and cilia play a critical role in the meiotic recombination process. Our study reveals the involvement of cilia in meiosis and suggests that extracellular signals may regulate meiotic recombination through this organelle.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jingqi Dai, Aurore Sanchez, Celine Adam, Lepakshi Ranjha, Giordano Reginato, Pierre Chervy, Carine Tellier-Lebegue, Jessica Andreani, Raphael Guerois, Virginie Ropars, Marie-Helene Le Du, Laurent Maloisel, Emmanuelle Martini, Pierre Legrand, Aurelien Thureau, Petr Cejka, Valerie Borde, Jean-Baptiste Charbonnier
Summary: The study characterized the crystal structure of Saccharomyces cerevisiae MutL gamma and identified its specificity in mismatch repair and meiotic recombination. The research also revealed clear divergent features between MutL homologs and identified, at the molecular level, their specialization towards MMR or meiotic recombination functions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Xiaoyi Wang, Melissa E. Pepling
Summary: This article discusses the meiotic process of female mammalian oocytes during embryonic development, highlighting the events and mechanisms from meiotic prophase one to metaphase two. It emphasizes the importance of understanding the mechanisms regulating meiotic progression to improve treatments for female infertility, as errors in meiosis can lead to aneuploidy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Genetics & Heredity
Sarah N. Ur, Kevin D. Corbett
Summary: Meiotic cell division is a specialized process preparing for sexual reproduction, involving homologous chromosomes identifying each other and forming physical links through DNA recombination. The SMC family cohesin complexes and meiotic chromosome axis play crucial roles in organizing chromosomes and promoting recombination. The synaptonemal complex (SC) ensures high fidelity in meiotic recombination by assembling between homologs and providing feedback.
ANNUAL REVIEW OF GENETICS, VOL 55
(2021)
Review
Cell Biology
Chengzi Huang, Ting Guo, Yingying Qin
Summary: POI is the early depletion of ovarian function before 40 years of age due to various causes, including genetic factors and unknown reasons. Advances in next-generation sequencing have expanded the genetic spectrum of POI, particularly in identifying genes related to meiosis and DNA repair. Understanding the genetic architecture of POI can help predict risks, protect ovarian function, and intervene early for affected women.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yang Li, Yufan Wu, Ihsan Khan, Jianteng Zhou, Yue Lu, Jingwei Ye, Junyan Liu, Xuefeng Xie, Congyuan Hu, Hanwei Jiang, Suixing Fan, Huan Zhang, Yuanwei Zhang, Xiaohua Jiang, Bo Xu, Hui Ma, Qinghua Shi
Summary: The study reveals that a splicing mutation in M1AP leads to decreased recombination intermediates and crossovers, impairing meiotic progression in males. M1AP acts as a copartner of the ZZS complex to promote crossover formation in males.
Article
Multidisciplinary Sciences
Masaru Ito, Asako Furukohri, Kenichiro Matsuzaki, Yurika Fujita, Atsushi Toyoda, Akira Shinohara
Summary: This study demonstrates that FIGNL1 plays a positive role in homologous recombination by controlling the non-productive assembly of RAD51/DMC1 on single-stranded and double-stranded DNAs, and promoting chromosome synapsis and meiotic DSB repair.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Xiang Li, Mingsen Yu, Pablo Bolanos-Villegas, Jun Zhang, Di'an Ni, Hong Ma, Yingxiang Wang
Summary: The study revealed the crucial role of FANCM in shaping the distribution of meiotic Class-I crossovers in plants, with evolutionary divergent roles observed between Arabidopsis and lettuce in meiotic bivalent formation.
Article
Environmental Sciences
Yi Hong, Xinyi Mu, Xingduo Ji, Xuemei Chen, Yanqing Geng, Yan Zhang, Qiqi Liu, Fangfang Li, Yingxiong Wang, Junlin He
Summary: This study revealed the adverse effects of HT-2 toxin on early oogenesis in fetal ovaries, including defects in meiotic prophase and increased oxidative stress. Pre-treatment with a natural compound with antioxidant activities partially reversed these effects.
ENVIRONMENTAL POLLUTION
(2021)
Article
Cell Biology
Alexandra Pyatnitskaya, Jessica Andreani, Raphael Guerois, Arnaud De Muyt, Valerie Borde
Summary: This study uncovers the functional link between crossover formation and synaptonemal complex (SC) assembly during meiotic recombination and identifies the molecular mechanism involved.
GENES & DEVELOPMENT
(2022)
Review
Genetics & Heredity
Lea Lingg, Sven Rottenberg, Paola Francica
Summary: Tumor cells often have genetic alterations that affect tumor progression and DNA repair. Reactivation of germ cell-specific genes in tumors influences DNA repair and response to treatment. Targeting aberrantly expressed meiotic genes may provide a unique opportunity to kill cancer cells specifically.
FRONTIERS IN GENETICS
(2022)
Editorial Material
Medicine, Research & Experimental
Jonas Schwickert, Franziska M. Zickgraf, Martin R. Sprick
Summary: Ovarian cancer, especially high-grade serous ovarian cancer (HGSOC), has the worst prognosis among all gynecological cancers. RAD51 expression is proposed as a biomarker of platinum resistance in HGSOC patients, which can help in selecting effective therapies for therapy-resistant patients.
EMBO MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Jacob Paiano, Nicholas Zolnerowich, Wei Wu, Raphael Pavani, Chen Wang, Hongzhi Li, Li Zheng, Binghui Shen, Barry P. Sleckman, Bo-Ruei Chen, Andre Nussenzweig
Summary: The study reveals that Pol alpha reduces resection at DSBs in the presence of 53BP1, but is unable to counteract excessive resection in the absence of 53BP1. This suggests that 53BP1 likely protects DNA ends by inhibiting resection.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Chao Liu, Wei Xu, Liying Wang, Zhuo Yang, Kuan Li, Jun Hu, Yinghong Chen, Ruidan Zhang, Sai Xiao, Wenwen Liu, Huafang Wei, Jia-Yu Chen, Qianwen Sun, Wei Li
Summary: During yeast meiosis, the frequency and distribution of R-loops, a type of chromatin structure, changed significantly. We found that multiple de novo R-loops were formed during the pachytene stage and were associated with meiotic recombination. The collisions between transcription and replication could promote the formation of R-loops during meiotic DNA replication, and these R-loops were associated with Spo11. Additionally, reversing the direction of transcription or replication could eliminate meiotic recombination hotspots, while reversing both directions could reconstitute the hotspots.
CELL AND BIOSCIENCE
(2023)
Article
Oncology
Nidal E. Muvarak, Khadiza Chowdhury, Limin Xia, Carine Robert, Eun Yong Choi, Yi Cai, Marina Bellani, Ying Zou, Zeba N. Singh, Vu H. Duong, Tyler Rutherford, Pratik Nagaria, Soren M. Bentzen, Michael M. Seidman, Maria R. Baer, Rena G. Lapidus, Stephen B. Baylin, Feyruz V. Rassoo
Meeting Abstract
Oncology
Feyruz V. Rassool, Nidal Muvarak, Khadiza Chowdury, Carine Robert, Limin Xia, Eun Yong Choi, Yi Cai, Marina Bellani, Ying Zou, Michael Seidman, Soren Bentzen, Maria Baer, Rena Lapidus, Stephen B. Baylin
Article
Multidisciplinary Sciences
Jing Huang, Himabindu Gali, Julia Gichimu, Marina A. Bellani, Durga Pokharel, Manikandan Paramasivam, Michael M. Seidman
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Biochemistry & Molecular Biology
Sanjay Kumar Bharti, Joshua A. Sommers, Sanket Awatet, Marina A. Bellani, Irfan Khan, Lynda Bradley, Graeme A. King, Yeonee Seol, Venkatasubramanian Vidhyasagar, Yuliang Wu, Takuye Abe, Koji Kobayashi, Kazuo Shin-ya, Hiroyuki Kitao, Marc S. Wold, Dana Branzei, Keir C. Neuman, Robert M. Brosh
NUCLEIC ACIDS RESEARCH
(2018)
Article
Genetics & Heredity
Marina A. Bellani, Jing Huang, Manikandan Paramasivam, Durga Pokharel, Julia Gichimu, Jing Zhang, Michael M. Seidman
Article
Cell Biology
Jing Huang, Jing Zhang, Marina A. Bellani, Durga Pokharel, Julia Gichimu, Ryan C. James, Himabindu Gali, Chen Ling, Zhijiang Yan, Dongyi Xu, Junjie Chen, Amom Ruhikanta Meetei, Lei Li, Weidong Wang, Michael M. Seidman
Article
Multidisciplinary Sciences
Jing Zhang, Marina A. Bellani, Ryan C. James, Durga Pokharel, Yongqing Zhang, John J. Reynolds, Gavin S. McNee, Andrew P. Jackson, Grant S. Stewart, Michael M. Seidman
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Jing Zhang, Jing Huang, Ryan C. James, Julia Gichimu, Manikandan Paramasivam, Durga Pokharel, Himabindu Gali, Marina A. Bellani, Michael M. Seidman
Summary: Considerable insight has been gained into cellular responses to double strand breaks (DSBs) caused by nucleases, radiation, and other DNA breakers. The study describes a strategy for tracking the cellular response to replication fork collisions with challenging adducts, such as DNA interstrand crosslinks (ICLs), using a steroid antigen linked to psoralen. By visualizing ICLs through immunofluorescence and utilizing the Proximity Ligation Assay (PLA), proteins closely associated with ICLs can be distinguished, allowing for the identification of replisome proteins retained or lost after encounters with ICLs. This approach can be applied to any immunologically detectable structure or DNA adduct.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Jing Zhang, Marina A. Bellani, Jing Huang, Ryan C. James, Durga Pokharel, Julia Gichimu, Himabindu Gali, Grant Stewart, Michael M. Seidman
Summary: During DNA replication, replisomes have different replication schedules in different chromatin environments, and their replication process can be influenced by replication stress. Currently, there is limited understanding of the determinants of replisome stalling vs. continued progression, but it has been discovered that replisomes with different activities exist in different stages and chromatin environments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Meeting Abstract
Environmental Sciences
S. Awate, S. K. Bharti, J. A. Sommers, M. Bellani, I Khan, L. Bradley, G. A. King, Y. Seol, V. Vidhyasagar, Y. Wu, T. Abe, K. Kobayashi, K. Shin-ya, H. Kitao, M. S. Wold, D. Branzei, K. C. Neuman, R. M. Brosh
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2018)
Meeting Abstract
Chemistry, Multidisciplinary
Manikandan Paramasivam, Marina Bellani, Julia Gichimu, Himabindu Gali, Michael Seidman
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Meeting Abstract
Oncology
Nidal Muvarak, Khadiza Chowdhury, Carine Robert, Xia Limin, Eun Yong Choi, Yi Cai, Marina Bellani, Michael Seidman, Maria R. Baer, Rena Lapidus, Stephen B. Baylin, Feyruz V. Rassool
Article
Biochemistry & Molecular Biology
Ji Jiang, Marina Bellani, Lin Li, Pengcheng Wang, Michael M. Seidman, Yinsheng Wang
ACS CHEMICAL BIOLOGY
(2017)
Article
Oncology
Limin Xia, Wenjie Huang, Marina Bellani, Michael M. Seidman, Kaichun Wu, Daiming Fan, Yongzhan Nie, Yi Cai, Yang W. Zhang, Li-Rong Yu, Huili Li, Cynthia A. Zahnow, Wenbing Xie, Ray-Whay Chiu Yen, Feyruz V. Rassool, Stephen B. Baylin
Review
Cell Biology
Robert M. Brosh, Marina Bellani, Yie Liu, Michael M. Seidman
AGEING RESEARCH REVIEWS
(2017)