期刊
NUCLEIC ACIDS RESEARCH
卷 41, 期 10, 页码 5251-5262出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt218
关键词
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资金
- Deutsche Forschungsgemeinschaft [SFB960]
- Bayerische Genomforschungsnetzwerk [BayGene]
- Elite Network of Bavaria
- German Research Foundation (DFG)
The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5's AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure.
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