期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 9, 页码 3939-3948出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq1347
关键词
-
资金
- 973 Project [2011CB936004]
- NSFC [20831003, 90813001, 20833006, 90913007]
- Chinese Academy of Sciences
As a promising strategy for artificially control of gene expression, reversible assembly of nanomaterials and DNA nanomachine, DNA triplex formation has received much attention. Carbon nanotubes as gene and drug delivery vector or as 'building blocks' in nano/microelectronic devices have been successfully explored. Therefore, studies on triplex DNA-based carbon nanotube hybrid materials are important for development of smart nanomaterials and for gene therapy. In this report, a small molecule directed single-walled carbon nanotubes (SWNTs) self-assembly assay has been developed by disproportionation of SWNTs-dT(22)circle dot dA(22) duplex into triplex dT(22)circle dot dA(22)circle dot dT(22) and dA(22) by a triplex formation inducer, coralyne. This has been studied by circular dichroism, light scattering (LS) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), electrophoretic mobility shift assay and supported by using DNA random sequence. In contrast, SWNTs do not aggregate under the same experimental conditions when the small molecules used can not induce dT(22)circle dot dA(22)circle dot dT(22) triplex formation. Therefore, this novel small molecule-directed SWNTs self-assembly assay has also been used for screening of triplex inducers in our studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据