期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 8, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq1280
关键词
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资金
- Japan Society for the Promotion of Science (JSPS)
- Kazusa DNA Research Institute
We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
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