Article
Biochemistry & Molecular Biology
Benjamin Ober-Reynolds, Winston R. Becker, Karina Jouravleva, Samson M. Jolly, Phillip D. Zamore, William J. Greenleaf
Summary: Studies have shown that TtAgo uses biophysical adaptations similar to those of eukaryotic Argonautes for rapid association but requires more extensive complementarity to achieve high-affinity target binding. The cleavage rates of TtAgo vary widely based on the DNA guide, suggesting that only a subset of guides cleaves targets on physiologically relevant timescales.
Article
Biochemistry & Molecular Biology
Takeshi Tabuchi, Yohei Yokobayashi
Summary: Cell-free systems without living cells are promising platforms for testing and practical applications. However, biological constraints limit the development of cell-free devices using living cells. This study presents a high-throughput screening method for engineering cell-free riboswitches that respond to small molecules.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder, Alexander van Oudenaarden
Summary: VASA-seq is a method that can detect the total transcriptome in single cells by fragmenting and tailing all RNA molecules after cell lysis. It is compatible with different experimental formats and can be used to analyze cell type markers, in vivo cell cycle, and alternative splicing, among others.
NATURE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ping Han, Maybelle K. Go, Jeng Yeong Chow, Bo Xue, Yan Ping Lim, Michael A. Crone, Marko Storch, Paul S. Freemont, Wen Shan Yew
Summary: This study introduces a semi-automated, kit-free RNA extraction method by adapting a well-established TRIzol-based protocol into a high-throughput format for SARS-CoV-2 detection, demonstrating scalability and cost-effectiveness.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jordan B. Metz, Nicholas J. Hornstein, Sohani Das Sharma, Jeremy Worley, Christian Gonzalez, Peter A. Sims
Summary: Protein synthesis is dysregulated in many diseases, and a systems-level understanding of how signaling molecules and RNA binding proteins interact with the translational machinery is lacking. This study presents a scalable method called riboPLATE-seq, which generates paired libraries of ribosome-associated and total mRNA, and demonstrates its utility in detecting translational alterations induced by specific protein kinase inhibitors.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Yan Zhang, Lei Kang, Ivy H. M. Wong, Weixing Dai, Xiufeng Li, Ronald C. K. Chan, Michael K. Y. Hsin, Terence T. W. Wong
Summary: CHAMP is a promising histological imaging method that enables high-throughput and label-free imaging of tissues with high resolution and processing speed. By unsupervised learning, CHAMP images can be transformed into virtually stained histological images, extracting significant cellular features with high accuracy.
Article
Multidisciplinary Sciences
Mert Boya, Tevhide Ozkaya-Ahmadov, Brandi E. Swain, Chia-Heng Chu, Norh Asmare, Ozgun Civelekoglu, Ruxiu Liu, Dohwan Lee, Sherry Tobia, Shweta Biliya, L. DeEtte McDonald, Bassel Nazha, Omer Kucuk, Martin G. Sanda, Benedict B. Benigno, Carlos S. Moreno, Mehmet A. Bilen, John F. McDonald, A. Fatih Sarioglu
Summary: The authors introduce a new technology, Cluster-Wells, which enables fast and accurate isolation and analysis of CTC clusters in blood, providing a potential tool for further studying the utility of these clusters in cancer management.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Yifu Lu, Zhuohan Yu, Yunhe Wang, Zhiqiang Ma, Ka-Chun Wong, Xiangtao Li
Summary: A novel Graph-based Multiple Hierarchical Consensus Clustering (GMHCC) method is developed in this study to handle clustering of various biomolecular data, showing high effectiveness. Experiments validate the method's efficiency and provide new insights into cell developmental lineages and characterization mechanisms.
Review
Nanoscience & Nanotechnology
Shizheng Zhou, Bingbing Chen, Edgar S. Fu, Hong Yan
Summary: This paper reviews the integration of microfluidic chips and computer vision in life sciences and biology research, specifically in the analysis of cell imaging data. Microfluidic chips generate large amounts of visual data at the single-cell level, while computer vision techniques rapidly process and analyze these data to extract valuable information about cellular health and function. This integrative approach allows for noninvasive and low-damage cellular characterization and provides a highly controlled environment for cell growth and manipulation, enhancing data analysis accuracy. It is expected that the combination of microelectromechanical devices, microfluidic chips, and computer vision will enable label-free, automatic, low-cost, and fast cellular information recognition and high-throughput analysis of cellular responses to different compounds for various applications in drug discovery, diagnostics, and personalized medicine.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Biochemical Research Methods
Qi Liu, Changjun Ding, Xiaoqiang Lang, Ganggang Guo, Jiafei Chen, Xiaohua Su
Summary: sRNAtools is a novel integrated platform for identifying and functionally annotating various sncRNAs, providing multiple modules and flexible research methods, supporting various upload options, including local versions.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Physics, Applied
Chenfei Hu, Mikhail E. Kandel, Young Jae Lee, Gabriel Popescu
Summary: Quantitative phase imaging (QPI) is a valuable label-free modality, but existing methods compromise between spatial resolution and field of view. Synthetic aperture interference light (SAIL) microscopy is a novel solution for high-resolution, wide field of view QPI, offering fast and accurate phase measurement without computational burden.
APPLIED PHYSICS LETTERS
(2021)
Article
Oncology
Sixian You, Eric J. Chaney, Haohua Tu, Yi Sun, Saurabh Sinha, Stephen A. Boppart
Summary: The computational pipeline allows for quantitative analysis of the tumor microenvironment, revealing extensive tissue reorganization and formation of a patterned cell-EV neighborhood in the cancerous tissue. This opens possibilities for better characterization and utilization of the evolving cancer landscape.
Article
Chemistry, Analytical
Fan Pu, Andrew J. Radosevich, James W. Sawicki, David Chang-Yen, Nari N. Talaty, Sujatha M. Gopalakrishnan, Jon D. Williams, Nathaniel L. Elsen
Summary: The study introduces a high-throughput mass spectrometry system based on infrared matrix-assisted desorption electrospray ionization (IR-MALDESI) for biochemical assays and compound screening. This system allows for fast data acquisition and reliable analysis without the need for chromatography, demonstrating its potential for high-throughput lead discovery assays.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Manish Tiwari, Baljinder Singh, Manisha Yadav, Vimal Pandey, Sabhyata Bhatia
Summary: Through sequencing and analyzing multiple miRNA and PARE libraries, the regulatory mechanism of miRNAs in chickpea root nodule formation was studied, revealing both conserved and novel miRNAs. Further phylogenetic analysis unveiled the ancestral relationships of these miRNAs. Studying the targeted genes of miRNAs and the changes in nodule numbers laid the foundation for further understanding the nodulation process in chickpea and other leguminous crops.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Materials Science, Multidisciplinary
Qiao Wu, Jun Liang, Jun-Dong Yi, Peng-Chao Shi, Yuan-Biao Huang, Rong Cao
SCIENCE CHINA-MATERIALS
(2019)
Article
Chemistry, Multidisciplinary
Qi Yin, Jian Lu, Hong-Fang Li, Tian-Fu Liu, Rong Cao
CRYSTAL GROWTH & DESIGN
(2019)
Article
Engineering, Environmental
Baohui Ren, Yanning Chen, Yanqiang Li, Weijin Li, Shuiying Gao, Hongfang Li, Rong Cao
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Multidisciplinary Sciences
Wanfeng Xiong, Hongfang Li, Hanhui You, Minna Cao, Rong Cao
NATIONAL SCIENCE REVIEW
(2020)
Article
Chemistry, Multidisciplinary
Ge Huang, Li Yang, Qi Yin, Zhi-Bin Fang, Xiao-Jing Hu, An-An Zhang, Jun Jiang, Tian-Fu Liu, Rong Cao
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Physical
Jingjun Li, Yanning Chen, Xue Yang, Shuiying Gao, Rong Cao
JOURNAL OF CATALYSIS
(2020)
Article
Chemistry, Physical
Qiao Wu, Rui-Kuan Xie, Min-Jie Mao, Guoliang Chai, Jun-Dong Yi, Shao-Shuai Zhao, Yuan-Biao Huang, Rong Cao
ACS ENERGY LETTERS
(2020)
Article
Chemistry, Inorganic & Nuclear
Wanfeng Xiong, Hongfang Li, Rong Cao
INORGANIC CHEMISTRY COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Yanning Chen, Baohui Ren, Shuiying Gao, Rong Cao
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Chemistry, Physical
Ying Hou, Yu-Lin Liang, Peng-Chao Shi, Yuan-Biao Huang, Rong Cao
APPLIED CATALYSIS B-ENVIRONMENTAL
(2020)
Article
Chemistry, Physical
Xue Yang, Suyuan Zhang, Huilin Tao, Xuyan Yan, Yanxi Tan, Yongfan Zhang, Shuiying Gao, Rong Cao
JOURNAL OF MATERIALS CHEMISTRY A
(2020)
Article
Chemistry, Physical
Hai-Bo Huang, Zhi-Bin Fang, Kai Yu, Jian Lu, Rong Cao
JOURNAL OF MATERIALS CHEMISTRY A
(2020)
Article
Chemistry, Multidisciplinary
Dongshuang Wu, Minna Cao, Hanhui You, Chuan Zhao, Rong Cao
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Inorganic & Nuclear
Jing-Xiang Lin, Yu-Xi Chen, Dan Zhao, Yu Chen, Xiu-Qiang Lu, Jian Lu, Rong Cao
INORGANIC CHEMISTRY FRONTIERS
(2019)
Article
Chemistry, Physical
Jun-Dong Yi, Rui Xu, Guo-Liang Chai, Teng Zhang, Ketao Zang, Bing Nan, Hua Lin, Yu-Lin Liang, Jiangquan Lv, Jun Luo, Rui Si, Yuan-Biao Huang, Rong Cao
JOURNAL OF MATERIALS CHEMISTRY A
(2019)