Article
Multidisciplinary Sciences
Aleksandar Radakovic, Saurja DasGupta, Tom H. Wright, Harry R. M. Aitken, Jack W. Szostak
Summary: Research shows that aminoacylated RNAs can assemble into chimeric amino acid-RNA polymers with active ribozyme function under template guidance, potentially introducing new functionalities in ribozyme catalysis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
David Smith, Geetha Kannan, Isabelle Coppens, Fengrong Wang, Hoa Mai Nguyen, Aude Cerutti, Einar B. Olafsson, Patrick A. Rimple, Tracey L. Schultz, Nayanna M. Mercado Soto, Manlio Di Cristina, Sebastien Besteiro, Vern B. Carruthers
Summary: This study indicates that genetic ablation of TgATG9 substantially reduces canonical autophagy and compromises the viability of T. gondii bradyzoites. Abnormal mitochondrial networks were observed in TgATG9-deficient bradyzoites, which led to compromised fitness both in vitro and in mice. Overall, TgATG9, and autophagy as a whole, play a critical role in maintaining cellular homeostasis in bradyzoites and are necessary for long-term persistence within the cyst of this coccidian parasite.
Article
Biology
Belen Garcia Pascual, Jan M. Nordbotten, Iain G. Johnston
Summary: Mitochondria and plastids rely on nuclear-encoded genes, but retain small subsets of oDNA. The energetic demands imposed by an organism's changing environment may influence the number of oDNA genes it retains. A mathematical model coupling cell processes and environmental dynamics predicts that species in dynamic environments retain more organelle genes, while those in less dynamic environments retain fewer. This is supported by oDNA data across eukaryotic taxa.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Review
Plant Sciences
Pengwei Wang, Patrick Duckney, Erlin Gao, Patrick J. J. Hussey, Verena Kriechbaumer, Chengyang Li, Jingze Zang, Tong Zhang
Summary: Functional regulation and structural maintenance of organelles in plants are crucial for plant development, reproduction, and stress responses. Recent studies have identified proteins that regulate membrane connections in plants, providing insights into the mechanism and function of these connections. The endoplasmic reticulum plays a key role in linking different subcellular compartments in plants, and its membrane contact sites (MCS) and ER-plasma membrane contact sites (EPCS) have been extensively studied. However, plant MCS are different from those in other eukaryotic systems. In this article, we summarize the recent advances in understanding these essential links in plants and discuss their mechanisms and biological relevance.
Article
Environmental Sciences
Lin-Lin Hu, Hong-Ge Li, Bi-Yun Liao, Yi Xu, Shao-Chen Sun, Jun-Li Wang
Summary: In this study, the effects of NP exposure on oocyte quality were investigated using a mouse in vivo model. The results showed that NP exposure caused changes in organelle distribution and function, leading to decreased oocyte development ability.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Melinda R. Grosser, Samantha K. Sites, Mayara M. Murata, Yolanda Lopez, Karen C. Chamusco, Kyra Love Harriage, Jude W. Grosser, James H. Graham, Fred G. Gmitter, Christine D. Chase
Summary: Plant genomes contain nuclear, plastid, and mitochondrial components which have different inheritance and evolutionary patterns. Genetic markers from these three genomes can be used to study inheritance, genetic relationships, and phenotypic contributions. Developing mitochondrial markers is challenging due to their high variability in size, gene order, and non-coding sequences, as well as their conservation in protein-coding sequences. PCR amplification of introns using conserved flanking exons is an effective method for developing polymorphic nuclear genome markers.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Materials Science, Biomaterials
Naoto Yoshinaga, Keiji Numata
Summary: Mitochondria are crucial for cellular metabolism and energy production, and mutations in mitochondrial DNA can lead to inherited diseases. Strategies targeting mitochondria for gene therapy have been developed, but further research and optimization are needed.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Review
Cell Biology
T. Bradley Willingham, Peter T. Ajayi, Brian Glancy
Summary: Mitochondria exhibit heterogeneity in form and function across different cell types and even within single cells. Distinct subpopulations of mitochondria have been identified in skeletal muscle cells, but the mechanisms by which this specialization contributes to energy homeostasis in working muscles remain unclear. Advances in cellular imaging technologies have opened up new potential avenues for further investigation into mitochondrial heterogeneity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
The Son Le, Mari Takahashi, Noriyoshi Isozumi, Akio Miyazato, Yuichi Hiratsuka, Kazuaki Matsumura, Tomohiko Taguchi, Shinya Maenosono
Summary: The efficient isolation of intact lysosomes is crucial for studying their functions and metabolites. A nanoparticle-based magnetic separation method was developed to target lysosomes using magnetic-plasmonic hybrid nanoparticles conjugated with amino dextran. The intracellular transport process of these nanoparticles was analyzed to determine the optimal conditions for magnetic isolation. The study found that the intactness of lysosomes can be quickly impaired, and optimal settings for high purity isolation were identified.
Review
Chemistry, Multidisciplinary
Si Si Liew, Xiaofei Qin, Jia Zhou, Lin Li, Wei Huang, Shao Q. Yao
Summary: This review summarizes recent developments and strategies in the smart design of nanocarriers for mitochondria-targeted therapeutics, including subcellular targeting, stimuli-responsive nanocarriers, and synergistic therapy, while also discussing existing challenges and unexplored therapeutic opportunities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Kira Muentjes, Senthil Kumar Devan, Andreas S. Reichert, Michael Feldbruegge
Summary: This article discusses the fundamental mechanisms of protein localization in eukaryotic cells and the importance of mRNA translation associated with endosomes for protein complex formation and accurate subcellular targeting, particularly for mitochondrial proteins. The novel link between endosomes and mitochondria adds a new level of complexity to organelle trafficking and biology, highlighting the significance of endosome-coupled translation in cellular processes.
Article
Chemistry, Multidisciplinary
Rashmi Yadav, Subrata Munan, Mudassar Ali, Koyeli Mapa, Animesh Samanta
Summary: A PET-based fluorescent probe, PM-C2, was developed for monitoring pH dynamics during mitochondrial dysfunctions. Compared to the other derivatives, PM-C2 showed higher fluorescence intensity and stronger pH sensitivity, making it suitable for tracking mitochondrial damage-induced pH alterations. The probe demonstrated biocompatibility, photostability, and mitochondria-specific targeting. The research was rated 8 out of 10.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Han Wang, Guiqian Fang, Huimin Chen, Maomao Hu, Yajuan Cui, Boyang Wang, Yudong Su, Yu Liu, Bo Dong, Xintian Shao
Summary: The study developed a new lysosome biosensor CNN for tracking lysosomes in living cells under both physiological and pathological conditions. High glucose conditions induced stress in lysosomes and altered the morphology of mitochondria.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Alexander G. Bury, Angela Pyle, Fabio Marcuccio, Doug M. Turnbull, Amy E. Vincent, Gavin Hudson, Paolo Actis
Summary: Intracellular heterogeneity is important for cellular physiology and diseases. This study developed a subcellular biopsy technology to isolate organelles, such as mitochondria, from human tissue. The technology was shown to be able to isolate organelles beyond the operational limit of laser capture microdissection (LCM) in human tissue.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Seo-Eun Lee, Young Cheol Kang, Yujin Kim, Soomin Kim, Shin-Hye Yu, Jong Hyeok Park, In-Hyeon Kim, Hyeon-Young Kim, Kyuboem Han, Hong Kyu Lee, Sung-Hwan Kim, Chun-Hyung Kim
Summary: Mitochondria play a vital role in cellular survival and their dysfunction contributes to various human diseases. This study reveals that exogenous mitochondria preferentially traffic to cells and tissues with damaged mitochondria, which has implications for therapeutic delivery to injured sites or diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ewa Toton, Aleksandra Romaniuk, Natalia Konieczna, Johann Hofmann, Jan Barciszewski, Maria Rybczynska
Article
Chemistry, Medicinal
Agnieszka Szymanska-Michalak, Dariusz Wawrzyniak, Grzegorz Framski, Jacek Stawinski, Jan Barciszewski, Adam Kraszewski
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Jan Barciszewski, Maria A. Ciemerych, Tomasz Twardowski
Article
Biochemistry & Molecular Biology
Blake A. Sweeney, Anton I. Petrov, Boris Burkov, Robert D. Finn, Alex Bateman, Maciej Szymanski, Wojciech M. Karlowski, Jan Gorodkin, Stefan E. Seemann, Jamie J. Cannone, Robin R. Gutell, Petra Fey, Siddhartha Basu, Simon Kay, Guy Cochrane, Kostantinos Billis, David Emmert, Steven J. Marygold, Rachael P. Huntley, Ruth C. Lovering, Adam Frankish, Patricia P. Chan, Todd M. Lowe, Elspeth Bruford, Ruth Seal, Jo Vandesompele, Pieter-Jan Volders, Maria Paraskevopoulou, Lina Ma, Zhang Zhang, Sam Griffiths-Jones, Janusz M. Bujnicki, Pietro Boccaletto, Judith A. Blake, Carol J. Bult, Runsheng Chen, Yi Zhao, Valerie Wood, Kim Rutherford, Elena Rivas, James Cole, Stanley J. F. Laulederkind, Mary Shimoyama, Marc E. Gillespie, Marija Orlic-Milacic, Ioanna Kalvari, Eric Nawrocki, Stacia R. Engel, J. Michael Cherry, Tanya Z. Berardini, Artemis Hatzigeorgiou, Dimitra Karagkouni, Kevin Howe, Paul Davis, Marcel Dinger, Shunmin He, Maki Yoshihama, Naoya Kenmochi, Peter F. Stadler, Kelly P. Williams
NUCLEIC ACIDS RESEARCH
(2019)
Review
Chemistry, Medicinal
Mohamed T. M. Nemr, Mahmoud N. M. Yousif, Jan Barciszewski
ARCHIV DER PHARMAZIE
(2019)
Article
Cell Biology
Adnan Khan Niazi, Etienne Delannoy, Rana Khalid Iqbal, Dania Mileshina, Romain Val, Marta Gabryelska, Eliza Wyszko, Ludivine Soubigou-Taconnat, Maciej Szymanski, Jan Barciszewski, Frederique Weber-Lotfi, Jose Manuel Gualberto, Andre Dietrich
Article
Biochemistry & Molecular Biology
Marek Kazimierczyk, Agata Jedroszkowiak, Dorota Kowalczykiewicz, Maciej Szymanski, Barbara Imiolczyk, Jerzy Ciesiolka, Jan Wrzesinski
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Marta Orlicka-Plocka, Dorota Gurda-Wozna, Agnieszka Fedoruk-Wyszomirska, Eliza Wyszko
Article
Biochemistry & Molecular Biology
Eliza Wyszko, Mariusz Popenda, Dorota Gudanis, Agnieszka Belter, Patrick Perrigue, Katarzyna Rolle, Jan Barciszewski, Joanna Sarzynska, Pawel Skowronek
Summary: Researchers have found that replacing RNA's D-ribose backbone with L-ribose isomers can increase its resistance to nucleases and improve its chances of survival in cells. They investigated the activity of left-handed hammerhead ribozyme and demonstrated the ability of L-ribose to reverse the chirality of RNA.
BIOSCIENCE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Belter, Mariusz Popenda, Marcin Sajek, Tomasz Wozniak, Miroslawa Z. Naskret-Barciszewska, Marta Szachniuk, Stefan Jurga, Jan Barciszewski
Summary: A new mechanism of RNA circularization driven by specific binding of miRNAs is described. MiR-7 RNAs bind to CDR1as RNA through the CUUCC pentanucleotide motifs, bringing both ends of CDR1as RNA together and stimulating phosphodiester bond formation. The proposed mechanism explains the specific RNA circularization and its function as a miRNAs sponge. Wet experimental data supports this observation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Forestry
Beata P. Plitta-Michalak, Miroslawa Z. Naskret-Barciszewska, Jan Barciszewski, Pawel Chmielarz, Marcin Michalak
Summary: The study found a significant increase in m(5)C level in pear tree seeds under conventional storage conditions, while a decrease was observed under cryogenic storage conditions, indicating that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings.
Article
Biochemistry & Molecular Biology
Blake A. Sweeney, Anton Petrov, Carlos E. Ribas, Robert D. Finn, Alex Bateman, Maciej Szymanski, Wojciech M. Karlowski, Stefan E. Seemann, Jan Gorodkin, Jamie J. Cannone, Robin R. Gutell, Simon Kay, Steven Marygold, Gil dos Santos, Adam Frankish, Jonathan M. Mudge, Ruth Barshir, Simon Fishilevich, Patricia P. Chan, Todd M. Lowe, Ruth Seal, Elspeth Bruford, Simona Panni, Pablo Porras, Dimitra Karagkouni, Artemis G. Hatzigeorgiou, Lina Ma, Zhang Zhang, Pieter-Jan Volders, Pieter Mestdagh, Sam Griffiths-Jones, Bastian Fromm, Kevin J. Peterson, Ioanna Kalvari, Eric P. Nawrocki, Anton S. Petrov, Shuai Weng, Philia Bouchard-Bourelle, Michelle Scott, Lauren M. Lui, David Hoksza, Ruth C. Lovering, Barbara Kramarz, Prita Mani, Sridhar Ramachandran, Zasha Weinberg
Summary: RNAcentral is a comprehensive database of non-coding RNA sequences, providing a single access point to over 18 million ncRNA sequences from diverse organisms and RNA types, as well as information on the secondary structure for over 13 million sequences. The database also includes a fast sequence similarity search tool with filtering options, making it a central data resource for the RNA community.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Joanna Kwiczak-Yigitbasi, Jean-Luc Pirat, David Virieux, Jean-Noel Volle, Agnieszka Janiak, Marcin Hoffmann, Jakub Mrzyglod, Dariusz Wawrzyniak, Jan Barciszewski, Donata Pluskota-Karwatka
Review
Biochemistry & Molecular Biology
Malgorzata Grabowska, Dariusz Wawrzyniak, Katarzyna Rolle, Piotr Chomczynski, Stefan Oziewicz, Stefan Jurga, Jan Barciszewski