Review
Immunology
Qifang Sun, Chengzhi Yuan, Sainan Zhou, Jing Lu, Meiyan Zeng, Xiong Cai, Houpan Song
Summary: Helicobacter pylori is a pathogen that causes various upper gastrointestinal diseases and has different pathogenic mechanisms. Accurate diagnosis and effective treatment are crucial for positive clinical outcomes, but there are challenges such as bacterial resistance and lack of preventive vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jason Tan, Yunhui Fang, Chuan Yang, Joyce Tay, Nathanael Tan, Nithiyaa Bala Krishnan, Boon Lin Chua, Yanli Zhao, Yunbo Chen, James L. Hedrick, Yi Yan Yang
Summary: This study presents the synthesis of new polymers capable of selectively killing Helicobacter pylori in the acidic environment of the stomach, while remaining non-toxic to commensal bacteria in the gut. The polymers demonstrated strong antibacterial activity against H. pylori in simulated gastric conditions, and their assembly behavior was influenced by the pH of the environment. The study provides valuable insights into the design and application of antimicrobial polymers.
Review
Microbiology
Seyedeh Zahra Bakhti, Saeid Latifi-Navid
Summary: The oral microbiota may play a role in the development and progression of gastric cancer, but the causal relationship between oral microbiota and gastric cancer remains controversial. Exploring the interaction and relationship between the oral microbiota and Helicobacter pylori in gastric cancer, as well as the impact of oral microbiota on the mechanisms of carcinogenesis, could offer new insights for the diagnosis and prevention of gastric cancer.
Article
Multidisciplinary Sciences
Jiashen Zhou, Lin Zhang, Liping Zeng, Lu Yu, Yuanyuan Duan, Siqi Shen, Jingyan Hu, Pan Zhang, Wenyan Song, Xiaoxue Ruan, Jing Jiang, Yinan Zhang, Lu Zhou, Jia Jia, Xudong Hang, Changlin Tian, Houwen Lin, Hong-Zhuan Chen, John E. Cronan, Hongkai Bi, Liang Zhang
Summary: The study presents the crystal structures of the essential UFA biosynthesis enzyme FabX in Helicobacter pylori, revealing a unique [4Fe-4S] cluster that potentiates FMN oxidation during dehydrogenase catalysis. This [4Fe-4S] cluster is crucial for unsaturated fatty acid synthesis and contributes to the pathogenic function of H. pylori in the corrosion of gastric mucosa.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Aimen Qaiser, Maria Hassan Kiani, Rashida Parveen, Muhammad Sarfraz, Gul Shahnaz, Abbas Rahdar, Pablo Taboada
Summary: This study successfully designed a hyaluronic acid-based nanocarrier system that improves the residence time of clarithromycin at the site of H. pylori infection, potentially enhancing the treatment efficacy.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Gastroenterology & Hepatology
Lingling Wang, Haobin Yao, Teresa Tong, KamShing Lau, Suet Yi Leung, Joshua W. K. Ho, Wai K. Leung
Summary: The study revealed dynamic alterations in gut microbiota and ARGs induced by various eradication therapies, which may have implications for antibiotic choices in eradication therapy.
Article
Chemistry, Organic
Chia-Chen Chang, Hau-Ming Jan, Chieh-Jen Tseng, Soumik Mondal, Andualem Bahiru Abera, Ming-Yen Hsieh, Tsai-Chen Yang, Sasikala Muthusamy, Sheng-Cih Huang, Chun-Hung Lin, Kwok-Kong Tony Mong
Summary: This study reports the isolation and stereochemical determination of the predominant native cholesteryl 6-O-phosphatidyl alpha- glucoside (CPG) from Helicobacter pylori. The authors used a combination of metabolic isolation and enzymatic degradation to obtain the native lactobacillic acid for stereochemical determination. The absolute stereochemistry of the acid was found to be 11R and 12S. Using this information, the authors achieved the total synthesis of predominant native CPG and other derivatives.
Article
Multidisciplinary Sciences
Afnan I. Shahin, Sumera Zaib, Seyed-Omar Zaraei, Reena A. Kedia, Hanan S. Anbar, Muhammad Tayyab Younas, Taleb H. Al-Tel, Ghalia Khoder, Mohammed I. El-Gamal
Summary: In this study, a new series of sulfonates and sulfamates bearing imidazo[2,1-b]thiazole scaffold were designed as potent inhibitors of urease enzyme. Compound 2c exhibited the highest inhibitory activity against urease, with an IC50 value of 2.94 +/- 0.05 mu M. Molecular modeling studies suggested multiple binding interactions between the most potent inhibitors and amino acid residues in the urease active site. Phenotypic screening showed that compounds 1a, 1d, 1h, 2d, and 2f had high potency against H. pylori.
Article
Chemistry, Multidisciplinary
Xiaopeng Zou, Jing Hu, Ming Zhao, Chunjun Qin, Yuntao Zhu, Guangzong Tian, Juntao Cai, Peter H. Seeberger, Jian Yin
Summary: Helicobacter pylori is a bacterium that colonizes the gastric mucosa of more than half of the world's population, causing chronic gastritis, peptic ulcers, and gastric cancer. Researchers have made significant progress in synthesizing the core undecasaccharide of H. pylori lipopolysaccharide, which is a promising candidate for developing glycoconjugate vaccines against multiple serotypes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Nadia A. Mohamed, Nahed A. Abd El-Ghany, Marwa M. Abdel-Aziz
Summary: Four new hydrogels were synthesized using chitosan and a cross-linker, with one showing potent inhibition against COX-2 and H. pylori. This hydrogel exhibited preferential inhibition towards COX-2 and could potentially serve as an anti-inflammatory and anti-H. pylori agent.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Jessica Rodrigues Pereira de Oliveira Borlot, Luna Schlittler dos Santos, Guilherme Jose Schwarzt Sampaio, Augusto Santos Borges, Ricardo Pereira Rodrigues, Rita de Cassia Ribeiro Goncalves, Reginaldo Bezerra dos Santos, Rodrigo Rezende Kitagawa
Summary: In this study, we synthesized and evaluated a series of 14 chalcones for their anti-H. pylori, NO inhibition, and cytotoxic effects. Compounds 3b and 3h showed good antibacterial activity and demonstrated structure-activity relationships. These compounds also inhibited the production of NO in LPS-stimulated macrophages and showed potential for interaction with the active site of the iNOS enzyme. Additionally, compounds 3b and 3h exhibited high selectivity towards AGS cell lines.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Eleonora Altman, Vandana Chandan, Blair A. Harrison, Melissa Schur, Marie-France Goneau, Jianjun Li, Michel Gilbert
Summary: This study investigated the use of glycoconjugates based on a recombinant alpha-1,6-glucosyltransferase from Helicobacter pylori to synthesize a linear alpha-1,6-glucan chain. The conjugates showed immunogenicity in rabbits and mice, inducing strong IgG responses against purified LPS from H. pylori strains. The post-immune sera from rabbits also exhibited bactericidal activity against selected clinical isolates of H. pylori.
Article
Biochemistry & Molecular Biology
Sandeepchowdary Bala, Kalisha Vali Yellamanda, Anilkumar Kadari, Venkata S. U. Ravinuthala, Bhavita Kattula, Om Singh, Rambabu Gundla, Anthony Addlagatta
Summary: The study analyzed MetAP sequences, identified a pattern of variation in the S1 pocket, and designed 17 selective inhibitors. These inhibitors selectively inhibited H. pylori MetAP compared to the human counterpart, with structural studies providing the molecular basis for the selectivity.
BIOORGANIC CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Minha Naseer, Rana Ramadan, Jianmin Xing, Nadia A. Samak
Summary: This study successfully synthesized copper oxide nanoparticles and confirmed their antibacterial activity against antibiotic-resistant strains, particularly Klebsiella pneumonia and Helicobacter pylori. The nanoparticles derived from Cassia fistula showed stronger inhibition of biofilm formation compared to those from Melia azedarach.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Chemistry, Multidisciplinary
Mark Reihill, Viviane Fourniere, Aisling Ni Cheallaigh, Johan Olofsson Edlund, Gavin John Miller, Thomas Boren, Martina Lahmann, Stefan Oscarson
Summary: In this study, the binding properties and preferences of BabA from different strains were evaluated by synthesizing B-antigen hexasaccharide and B-Lewis b heptasaccharide glycoconjugates with Bovine Serum Albumin. The results showed that the presence of the B determinant in the Bleb7-conjugates reduced the binding to high affinity H. pylori strains, and the absence of the Lewis fucose residue in the B6-conjugates further reduced the binding.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Microbiology
Andree F. Maheux, Dominique K. Boudreau, Jehane Y. Abed, Eve Berube, Stephanie Brodeur, Kathryn A. Bernard, Ameena Hashimi, Eloise Ducrey, Emilie F. Guay, Frederic Raymond, Jacques Corbeil, Marc-Christian Domingo, Paul H. Roy, Maurice Boissinot, Elitza Tocheva, Rabeea F. Omar
Summary: A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis. Based on phylogenetic analysis, chemotaxonomy, and other phenotypic properties, strain CCRI-22567(T) represents a new genus and species within the family Peptostreptococcaceae, named Criibacterium bergeronii gen. nov., sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Moira B. Dion, Pier-Luc Plante, Edwige Zufferey, Shiraz A. Shah, Jacques Corbeil, Sylvain Moineau
Summary: A large number of new phages have been discovered through viral metagenomics, with their host prediction based on CRISPR spacers which represent past phage-bacteria interactions. A set of tools has been developed for predicting hosts of uncharacterized phages, utilizing a database of over 11 million spacers and a program for large viral datasets. Performance evaluations show promise for gut-virome characterization using this host prediction method.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Diana Ascencio, Guillaume Diss, Isabelle Gagnon-Arsenault, Alexandre K. Dube, Alexander DeLuna, Christian R. Landry
Summary: Most protein complex subunits have small to nondetectable fitness effects when duplicated, with few exceptions. Very few gene duplications affect both fitness and protein-protein interactions. Large complexes like the 26S proteasome are protected from gene duplication by regulating protein abundance.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Anne D. Jungblut, Frederic Raymond, Moira B. Dion, Sylvain Moineau, Vani Mohit, Guillaume Quang Nguyen, Maxime Deraspe, Elina Francovic-Fontaine, Connie Lovejoy, Alexander I. Culley, Jacques Corbeil, Warwick F. Vincent
Summary: This study conducted the first genomic analysis of the Nostoc commune morphotype in the Arctic, revealing higher diversity of secondary metabolites and diverse interactions with viruses in polar Nostoc.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Etienne Racine, Guy Boivin, Yves Longtin, Deirdre McCormack, Helene Decaluwe, Patrice Savard, Matthew P. Cheng, Marie-Eve Hamelin, Julie Carbonneau, Fazia Tadount, Kelsey Adams, Benoite Bourdin, Sabryna Nantel, Vladimir Gilca, Jacques Corbeil, Gaston De Serres, Caroline Quach-Thanh
Summary: Among unvaccinated healthcare workers, reinfection with SARS-CoV-2 following a primary infection remained rare. The duration of seropositivity was significantly longer in symptomatic individuals compared to asymptomatic ones.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2022)
Article
Immunology
Olus Uyar, Juan Manuel Dominguez, Maude Bordeleau, Lina Lapeyre, Fernando Gonzalez Ibanez, Luc Vallieres, Marie-Eve Tremblay, Jacques Corbeil, Guy Boivin
Summary: A new microglia-like state with specific gene expressions associated with neutrophils has been identified in herpes simplex encephalitis (HSE), suggesting an exacerbated CNS inflammation due to infected microglia/microglia-like cells producing increased IL-1β through NLRP3-inflammasome-mediated pathway.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Multidisciplinary Sciences
Andre J. Faure, Julia Domingo, Jorn M. Schmiedel, Cristina Hidalgo-Carcedo, Guillaume Diss, Ben Lehner
Summary: This paper presents a method for globally mapping protein allostery using deep mutational scanning. The approach is applied to two common protein interaction domains in humans, revealing that mutations closer to binding interfaces, at specific residues, and at residues connecting to an opposite surface within the domain are more likely to be allosteric.
Article
Multidisciplinary Sciences
Maxime Deraspe, Sebastien Boisvert, Francois Laviolette, Paul H. Roy, Jacques Corbeil
Summary: Protein identification is a computationally intensive step in genomics studies. This study introduces kAAmer, a protein database engine that efficiently identifies proteins and allows for flexible protein annotations. The database can also be used as a microservice, hosted and queried remotely.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Emilie Gobeil, Ina Maltais-Payette, Nele Taba, Francis Briere, Nooshin Ghodsian, Erik Abner, Jerome Bourgault, Eloi Gagnon, Hasanga D. Manikpurage, Christian Couture, Patricia L. Mitchell, Patrick Mathieu, Francois Julien, Jacques Corbeil, Marie-Claude Vohl, Sebastien Theriault, Tonu Esko, Andre Tchernof, Benoit J. Arsenault
Summary: This study found a potential causal effect of NAFLD on blood tyrosine levels, suggesting it may serve as a new biomarker for NAFLD.
Article
Biochemistry & Molecular Biology
Ina Maltais-Payette, Jinchu Vijay, Marie-Michelle Simon, Jacques Corbeil, Francis Briere, Elin Grundberg, Andre Tchernof
Summary: This study found an association between circulating glutamate levels and abdominal obesity, which is related to dysregulated GLUL expression in visceral adipose tissue.
Article
Infectious Diseases
Paul B. L. George, Florent Rossi, Magali-Wen St-Germain, Pierre Amato, Thierry Badard, Michel G. Bergeron, Maurice Boissinot, Steve J. Charette, Brenda L. Coleman, Jacques Corbeil, Alexander Culley, Marie-Lou Gaucher, Matthieu Girard, Stephane Godbout, Shelley P. Kirychuk, Andre Marette, Allison McGeer, Patrick T. O'Shaughnessy, E. Jane Parmley, Serge Simard, Richard J. Reid-Smith, Edward Topp, Luc Trudel, Maosheng Yao, Patrick Brassard, Anne-Marie Delort, Araceli D. Larios, Valerie Letourneau, Valerie E. Paquet, Marie-Helene Pedneau, Emilie Pic, Brooke Thompson, Marc Veillette, Mary Thaler, Ilaria Scapino, Maria Lebeuf, Mahsa Baghdadi, Alejandra Castillo Toro, Amelia Belanger Cayouette, Marie-Julie Dubois, Alicia F. Durocher, Sarah B. Girard, Andrea Katherin Carranza Diaz, Asmaa Khalloufi, Samantha Leclerc, Joanie Lemieux, Manuel Perez Maldonado, Genevieve Pilon, Colleen P. Murphy, Charly A. Notling, Daniel Ofori-Darko, Juliette Provencher, Annabelle Richer-Fortin, Nathalie Turgeon, Caroline Duchaine
Summary: Antimicrobial resistance (AMR) is a growing global problem that affects public health, agriculture, and the environment. The mechanism of aerial transport of antimicrobial-resistant bacteria via bioaerosols is not well understood. This ongoing project in Canada aims to assess the presence and fate of AMR in bioaerosols, including the emissions of antibiotic resistance genes, their long-distance transport, and dissemination models. A multidisciplinary approach is necessary to understand the role of bioaerosols in the spread of AMR.
Article
Immunology
Sabryna Nantel, Benoite Bourdin, Kelsey Adams, Julie Carbonneau, Henintsoa Rabezanahary, Marie-Eve Hamelin, Deirdre McCormack, Patrice Savard, Yves Longtin, Matthew P. Cheng, Gaston De Serres, Jacques Corbeil, Vladimir Gilca, Mariana Baz, Guy Boivin, Caroline Quach, Helene Decaluwe
Summary: Public health vaccination recommendations for COVID-19 primary series and boosters in previously infected individuals differ worldwide. However, this study provides detailed immunological evidence that one- or two-dose primary vaccination series is appropriate for individuals with previous infection. These findings highlight the importance of administering the complete two-dose primary regimen and following boosters of mRNA vaccines to individuals who experienced asymptomatic SARS-CoV-2 infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Jehane Y. Abed, Thibaud Godon, Fadwa Mehdaoui, Pier-Luc Plante, Maurice Boissinot, Michel G. Bergeron, Richard E. Belanger, Gina Muckle, Natalia Poliakova, Pierre Ayotte, Jacques Corbeil, Elsa Rousseau
Summary: This study used comparative metagenomics to investigate the unique gut microbiome of Nunavik Inuit, revealing a higher diversity and distinctive taxonomic and functional characteristics compared to other populations. The findings indicate a potentially important role of gut microbiome in the health of Nunavik Inuit.
COMMUNICATIONS BIOLOGY
(2022)
Article
Infectious Diseases
Eliel Brochu, Ann Huletsky, Dominique K. Boudreau, Frederic Raymond, Eve Berube, Amin Ahmed Ouameur, Johanne Frenette, Maurice Boissinot, Jacques Corbeil, Michel G. Bergeron
Summary: This study characterized vancomycin-resistance vanD gene clusters and potential vanD-carrying bacteria in the intestinal microbiota of healthy volunteers exposed or not exposed to beta-lactam antibiotics. The culture-enriched metagenomic sequencing allowed detection of vanD genes, which were not detected by direct metagenomics. The study also revealed that commensal anaerobic bacteria could be the reservoir of vanD genes carried by enterococci.
JAC-ANTIMICROBIAL RESISTANCE
(2023)
Article
Genetics & Heredity
Yuan Zeng, Yuqin Song, Lanqing Cui, Qi Wu, Chao Wang, Adriano Cappellazzo Coelho, Gang Zhang, Dawei Wei, Chao Li, Jingren Zhang, Jacques Corbeil, Yun Li, Jie Feng
Summary: This study investigated the evolutionary trajectories of multidrug-resistant Streptococcus pneumoniae CC271 in China. The results identified two globally distributed clones, ST271-A and ST271-B, with the latter being the most prevalent clone in China. The study also observed rapid expansion and international transmission of different clones, and the influence of vaccination on clone prevalence.
